Identification of β-hydroxybutyrate as a potential biomarker for female papillary thyroid cancer

Abstract Background Papillary thyroid cancer (PTC) is the most common malignancy of all thyroid cancers. LncRNA LINC00460 has been proved to play roles in the oncogenesis and progression of various tumors, including papillary thyroid cancer. However, the potential molecular mechanism of LINC00460 in PTC is poorly investigated. Results LINC00460 was upregulated in PTC tissues and cells. Raf1 was upregulated in PTC tissues, but miR-485-5p was down-regulated. High LINC00460 expression was associated with poor prognosis. LINC00460 knockdown suppressed proliferation, migration, invation and EMT of PTC cells. Bioinformatics prediction revealed that LINC00460 had binding sites with miR-485-5p, which was validated by luciferase reporter assay. In addition, miR-485-5p was confirmed to directly target Raf1 3′-UTR. Moreover, LINC00460 promoted PTC progression by sponging miR-485-5p to elevate the expression of Raf1. Knockdown of LINC00460 restrained tumor growth in vivo. Conclusion LINC00460 induced proliferation, migration, invation and EMT of PTC cells by regulating the LINC00460/miR-485-5p/Raf1 axis, which indicated that LINC00460 may be a potential biomarker and therapeutic target for PTC.

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Down-regulation of DANCR acts as a potential biomarker for papillary thyroid cancer diagnosis

Bioscience Reports ◽

10.1042/bsr20181616 ◽

2019 ◽

Vol 39 (4) ◽

Cited By ~ 4

Author(s):

Ke Zhang ◽

Jing Lv ◽

Xiaowei Peng ◽

Jianqiu Liu ◽

Cuilin Li ◽

...

Keyword(s):

Thyroid Cancer ◽

Papillary Thyroid Cancer ◽

Expression Profiles ◽

Expression Level ◽

Papillary Thyroid ◽

Normal Tissues ◽

Cancer Breast ◽

Tumor Tissues ◽

Potential Biomarker ◽

Reverse Transcription Quantitative Pcr

AbstractLong non-coding RNAs (lncRNAs) have been reported to be dysregulated and play a crucial role in the progression of cancer. LncRNA DANCR has recently been revealed to be involved in tumorigenesis of numerous types of cancer, including osteosarcoma, gastric cancer, breast cancer, hepatocellular carcinoma, and colorectal cancer. However, the expression profiles and biological relevance of DANCR in papillary thyroid cancer (PTC) have not yet been reported. In the present study, the expression level of DANCR in PTC tissues and adjacent normal tissues was detected by reverse transcription-quantitative PCR in PTC patients, and then we analyzed the association with clinical pathological characteristics of patients and DANCR expressions. These results demonstrated that the expression of DANCR was notably decreased in tumor tissues in comparison with adjacent normal tissues (P<0.001). Furthermore, the present study found that DANCR expression level was correlated to T grade (P<0.01) and TNM stage (P=0.017). The present study demonstrated that DANCR was associated with PTC aggressive clinical features and may serve as a diagnostic biomarker for detecting PTC patients.

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DIO3OS as a potential biomarker of papillary thyroid cancer

Pathology - Research and Practice ◽

10.1016/j.prp.2021.153695 ◽

2021 ◽

pp. 153695

Author(s):

Ye Wang ◽

Junfu Wang ◽

Congjun Wang ◽

Yeyang Chen ◽

Junqiang Chen

Keyword(s):

Thyroid Cancer ◽

Papillary Thyroid Cancer ◽

Papillary Thyroid ◽

Potential Biomarker

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Expression of Caveolin-1 Is Associated With Thyroid Function in Patients With Human Papillary Thyroid Carcinoma

Dose-Response ◽

10.1177/1559325820919330 ◽

2020 ◽

Vol 18 (2) ◽

pp. 155932582091933

Author(s):

Jingyi Zhang ◽

Dongxia Yan ◽

Lianping He ◽

Qing Zhang ◽

Shuang Wen ◽

...

Keyword(s):

Thyroid Cancer ◽

Epithelial Cells ◽

Thyroid Nodule ◽

Papillary Thyroid Cancer ◽

Thyroid Function ◽

Follicular Adenoma ◽

Papillary Thyroid ◽

Follicular Thyroid Cancer ◽

Caveolin 1 ◽

Potential Biomarker

Objective: The aim of this study was to evaluate the levels of caveolin-1 in thyroid follicular epithelial cells of papillary thyroid cancer, follicular thyroid cancer, and nonmalignant thyroid nodule benign follicular adenoma, as well as to explore the relationship between the levels of caveolin-1 and thyroid function. Methods: Thirty cases of papillary thyroid cancer, 10 cases of follicular thyroid cancer, 32 cases of nonmalignant thyroid nodule benign follicular adenoma, and 30 controls were enrolled in this study. Caveolin-1 expression in tissue specimens obtained from these cases was evaluated by immunohistochemistry and Western blotting. Results: Caveolin-1 expression in thyroid epithelial cells of patients with papillary thyroid cancer, particularly female patients, was significantly higher than that in patients with follicular thyroid cancer and nonmalignant thyroid nodule benign follicular adenoma ( P < .005). Serum thyroid-stimulating hormone (TSH) levels in the caveolin-1-positive expression group were lower than that in the caveolin-1-negative expression group, and the lowest expression of caveolin-1 was detected in tissues of patients with Graves’ disease. The serum TSH level was associated with caveolin-1 expression in thyroid epithelial cells. Conclusion: Caveolin-1 may participate in regulating thyroid function and is a potential biomarker of follicular thyroid cancer.

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CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

Bioscience Reports ◽

10.1042/bsr20203459 ◽

2020 ◽

Author(s):

Xiao-Jing Qin ◽

Xu Lin ◽

Gang Xue ◽

Hui-Li Fan ◽

Hao-Yu Wang ◽

...

Keyword(s):

Immune Response ◽

Thyroid Cancer ◽

Tumor Microenvironment ◽

Papillary Thyroid Cancer ◽

Critical Role ◽

Functional Enrichment ◽

Detection Methods ◽

Pathway Enrichment Analysis ◽

Papillary Thyroid ◽

Potential Biomarker

Background: In recent years, the annual incidence of thyroid cancer (TC) has increased, with papillary thyroid cancer (PTC) identified as the most common pathological type accounting for about 80% of all thyroid cancer cases. The tumor microenvironment is known to play a vital role in tumor information transmission and immune detection. Methods: In this study, we examined gene expression data from 518 patients with PTC. The ESTIMATE algorithm was used to calculate immune and stromal scores of PTC patients. Based on a protein-protein interaction (PPI) network, functional enrichment and overall survival analyses, C-X-C motif chemokine ligand 10 (CXCL10) was identified as a core gene. We further investigated the roles of core genes of PTC in the tumor immune microenvironment using LinkedOmics, GSEA, and TIMER tools. Results: Immune, stromal and ESTIMATE scores were related to clinicopathological variables of patients with PTC, but not survival outcomes. Eight differentially expressed genes (DEGs) were associated with survival outcome. In addition, immunochemical staining experiments revealed lower expression of CXCL10 in PTC than paracancerous tissues. GSEA pathway enrichment analysis revealed downregulation of CXCL10 in multiple cancer pathways. CXCL10 and related genes were enriched in pathways related to adaptive immune response, cellular defense response and regulation of innate immune response. Conclusion: The tumor microenvironment plays a critical role in development of PTC and CXCL10 may serve as a novel target of precision therapy for this patient population.

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