In Vitro Studies on Phytochemical Screening of Different Leaf Extracts and Their Antifungal Activity against Seed Borne Pathogen Aspergillus niger

2015 ◽  
Vol 6 (11) ◽  
Author(s):  
Mohammad Danish ◽  
Hisamuddin ◽  
Merajul Islam Robab
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
In Vitro Studies ◽  
Phytochemical Screening ◽  
Leaf Extracts

Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

2017 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Michael Russelle Alvarez ◽  
Paolo Robert Bueno ◽  
Raymond Oliver Cruz ◽  
Richard Macapulay ◽  
Francis Jayson Vallesfin ◽  
...  
Keyword(s):  
Crude Extract ◽  
Carica Papaya ◽  
Uv Light ◽  
Digestive Enzyme ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Salivary Amylase ◽  
Garcinia Mangostana ◽  
Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. ­Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested ­positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

IN VITRO ANTIMICROBIAL ACTIVITY OF CYNODON DACTYLON (L.) PERS. LEAF EXTRACTS. ANTIMICROBIAL ACTIVITY BY ZONE OF INHIBITION ESTIMATION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 37-41
Author(s):  
S. M Pawaskar ◽  
◽  
K. C. Sasangan
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cynodon Dactylon ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
In Vitro Antimicrobial Activity

The present study was undertaken to evaluate in vitro antimicrobial activity of the successive leaf extracts of Cynodon dactylon in petroleum ether, ethyl acetate, acetone, ethanol, methanol and water, against various gram positive & gram negative bacterial strains using zone of inhibition. Both Agar well diffusion method & Agar disc diffusion method were used to evaluate the antibacterial efficacy. The microorganisms used in the test were – Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella flexneri, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Bacillus subtilis, Streptococcus pyogenes, Vibrio cholerac and Enterobacter aerogenes. Two fungal strains - Candida albicans and Saccharomyces cerevisiae were also tested to evaluate the antifungal potential of the said plant extract. The reference antibiotics chloramphenicol & ampicillin (antibacterial); nystatin & clotrimazole and (antifungal) were also tested against these standard microorganisms used in the assay and the results were compared with that of the plant extracts.The results of the study revealed that all the seven successive extracts of the leaf powder of Cynodon dactylon ( L.) Pers. exhibited prominent antimicrobial and antifungal activity against all microorganisms used in the study. The nonpolar extracts i.e. petroleum ether, ethyl acetate and acetone showed the most significant antibacterial and antifungal activity against all tested organisms. The petroleum ether and ethyl acetate extracts showing maximum inhibition in the range of 8 mm - 15 mm. This was closely followed by, acetone extract, which showed the inhibition in the range of 9 mm - 13 mm.

scholarly journals In vitro antifungal activity of Dorstenia mannii leaf extracts (Moraceae)

2016 ◽  
Vol 15 (3) ◽  
pp. 62-67
Author(s):  
Frederick Agem Kechia ◽  
Ngo Teke Gerald ◽  
Bathelemy Ngameni ◽  
Charles Fokunang ◽  
Jean Paul Dzoyem ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Leaf Extracts ◽  
In Vitro Antifungal Activity

scholarly journals Antioxidants and Aspergillus niger Antifungal Activity in vitro of Flavonoids Extract from Red-Top Leaves of Cratoxylum prunifolium

2019 ◽  
Vol 31 (7) ◽  
pp. 1441-1446
Author(s):  
Tran Dinh Manh ◽  
Le Thi Them ◽  
Duy Chinh Nguyen ◽  
Hieu Vu-Quang ◽  
Le Si Ngoc
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger

scholarly journals Phytochemical screening and antifungal potency of Vernonia amygdalina (bitter leaf) extract against post harvest mycodeterioration of tomato (Lycopersicum esculentum)

2020 ◽  
Vol 7 (17) ◽  
pp. 327-337
Author(s):  
U. N. Emiri ◽  
E. B. Enaregha
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Diffusion Method ◽  
Lycopersicum Esculentum ◽  
Phytochemical Screening ◽  
Rhizopus Stolonifer ◽  
Vernonia Amygdalina ◽  
Tomato Fruits ◽  
Post Harvest ◽  
Ethanolic Extracts

The study investigated fungi associated with post harvest tomato fruits sold in the open market in Port Harcourt Metropolis. The antifungal activity (bitter leaf) extracts against spoilage was studied using well-in-agar diffusion method. Results showed that mean percentage incidence of fungi isolated from tomato fruits were Rhizopus stolonifer (56%), Aspergillus niger (62%) and Altermaria altermata (35%). Aqueous and ethanolic extracts of Vernonia amygdalina at different concentrations (20%, 40%, and 60%) (w/v) were used against fungi isolates, while water and ketoconzole (0.5 mg/mL) served as negative and positive control, respectively. Aqueous and ethanolic extracts of V. amygdalina inhibited the growth of all three test fungi. There were significant differences (p > 0.05) in the mean inhibitory effects of plant extracts and control. Antifungal activity measured as diameter of zone of inhibition revealed that V. amygdalina acqueous extract at 60% was very active against Aspergillus niger (16.50 mm) and Alternaria altermata (16.00 mm), while being moderately active against Rhizopus stolonifer (13.00 mm). However, 60% ethanolic extract of V. amygdalina was very active against A. niger (19.00 mm), A. altermata (17.00 mm) and Rhizopus stolonifer (15.80 mm). Phytochemical screening of V. amygdalina revealed the presence of tannins, oxalate, saponnins, flavanoid, cynogenic glucoside, phytate and Alkaloids V. amygdalina could serve as a potentially viable alternative to chemical fungicides in the preservation of post harvest tomato fruits (Lycopersicum esculentum).

scholarly journals IN-VITROSTUDIES ON ANTIFUNGALACTIVITY OF SOMEMISTLETOE SPECIES

2021 ◽  
Vol 9 (06) ◽  
pp. 420-427
Author(s):  
R. Neelima ◽  
◽  
B. Sujatha ◽  
N.V. Ramana ◽  
B.V. Rao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Phytophthora Infestans ◽  
Inhibitory Activity ◽  
Sclerotium Rolfsii ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Leaf Extracts

In vitro evaluation of antifungal activity by disc diffusion method was carried out on leaf extracts of D.falcata, D. falcata var pubescens, V. monoicum and V. orientale and stem extracts of D. falcata, D. falcata var pubescens, V. articulatum and V.orientale using three different solvents viz., methanol, n-hexane and ethyl acetate. The antifungal activity was tested on three fungal strains include Fusarium oxysporum, Phytophthora infestans, Sclerotium rolfsii. Overall leaf extracts exerted better inhibitory activity than stem counterparts. Among all, only V. orientale was effective against the three fungal species.Compared to stem extracts, leaf extracts of D. falcata var pubescens have shown higher antifungal activity.

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