Evaluation of Factors Impacting Agrobacterium-Mediated Indica Rice Transformation of IR58025B - a Public Maintainer Line

At the cellular level, the Salt Overly Sensitive (SOS) signaling pathway comprising SOS3, SOS2, and SOS1 has been proposed to mediate cellular signaling under salt stress to maintain ion (Na+) homeostasis. In this regulatory pathway, both OsSOS1 encoding plasma membrane and OsNHX1 encoding vacuolar Na+/H+ antiporters are regulated by SOS3?SOS2 protein kinase complex. In the present study, the rice variety BRRI dhan28 - which is popular with farmers and high yielding, but salt sensitive, was transformed with the OsSOS1 gene isolated from salt tolerant Pokkali rice and driven by the constitutive promoter, CaMV35S. The construct was transformed through a tissue culture-independent Agrobacteriummediated in planta transformation method that circumvents the problems associated with tissue culture-based indica rice transformation methods. Integration of the foreign genes (OsSOS1) into the genome of transgenic plants was confirmed by gene-specific PCR and Southern blot analysis. The level of transgene expression (SOS1) was also quantified by semi-quantitative RT PCR and real time PCR. Genetic segregation ratio for T1 progenies was calculated and found to follow the Mendelian law of inheritance in case of positive transformants. The transformants were shown to be salt tolerant compared to wild type in molecular analysis as well as physiological screening. Future work will involve transformation of both the OsSOS1 and OsNHX1 genes together; with the expectation for enhancing the tolerance level compared to currently available transgenic rice.Plant Tissue Cult. & Biotech. 25(2): 257-272, 2015 (December)

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Overexpression of Sugarcane Sucrose Transporter 1 (SoSUT1) Gene Increases Rice Yield

ANNALES BOGORIENSES ◽

10.14203/ann.bogor.2020.v24.n1.18-26 ◽

2020 ◽

Vol 24 (1) ◽

pp. 18

Author(s):

Cesha Ananda Putri ◽

Bambang Soegiharto ◽

Parawita Dewanti

Keyword(s):

Rice Yield ◽

Indica Rice ◽

Sucrose Transporter ◽

Pcr Analysis ◽

Panicle Length ◽

Rice Transformation ◽

Rice Plants ◽

Apical Bud ◽

Source To Sink ◽

Spherical Grains

The growth and development of plants are determined by photosynthesis, which ultimately results in sucrose. Sucrose is synthesized in the source then translocated to all parts of the plant (sink). The translocation process of sucrose from source to sink is controlled by sucrose protein called sucrose transporter. SoSUT1 is a gene that encodes a sucrose transporter 1 (SUT1) protein in sugarcane. Rice transformation with the SoSUT1 causes overexpression SUT, which is expected to increase the translocation of sucrose into the seed of rice plants. This research was conducted by introducing SoSUT1 in rice plants Inpari 14 SS. Transformation using Agrobacterium tumefaciens vector in apical bud explant Indica rice cv. Inpari 14 SS results in 26 events positive rice contains genes SoSUT1. This study aims to elucidate the inheritance of transgene in the next generation and to characterize its effect on the morphology and the yields of a subsequent generation. The study is conducted by planting the seeds of T1 and T2 plants in media containing Hygromycin and using PCR analysis for further analysis. As the results, from 26 events on the T1 plant, only 3 events of T3 plants were confirmed on the T3 plant. The overexpression of the SoSUT1 gene could increase the number of tillers, the number of productive tillers, panicle length, and panicle exit length, also increasing the number of spherical grains, reducing the number of empty grains and increasing the weight of 1000 grains.

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A common wild rice-derived BOC1 allele reduces callus browning in indica rice transformation

Nature Communications ◽

10.1038/s41467-019-14265-0 ◽

2020 ◽

Vol 11 (1) ◽

Cited By ~ 4

Author(s):

Kun Zhang ◽

Jingjing Su ◽

Min Xu ◽

Zhihui Zhou ◽

Xiaoyang Zhu ◽

...

Keyword(s):

Wild Rice ◽

Indica Rice ◽

Rice Transformation ◽

Common Wild Rice ◽

Callus Browning

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Genetic analysis and molecular mapping of eui1 gene conferring elongated uppermost internode in the indica rice mutant Xieqingzao eB1

Chinese Journal of Agricultural Biotechnology ◽

10.1079/cjb200418 ◽

2004 ◽

Vol 1 (2) ◽

pp. 103-107 ◽

Cited By ~ 5

Author(s):

Ma Hong-Li ◽

Zhang Shu-Biao ◽

Lu Qin ◽

Fang Xuan-Jun ◽

Yang Shu-Lan ◽

...

Keyword(s):

Genetic Analysis ◽

Molecular Mapping ◽

Indica Rice ◽

Recessive Gene ◽

Mutant Gene ◽

Genetic Distances ◽

Maintainer Line ◽

Γ Radiation ◽

Rice Mutant ◽

Uppermost Internode

AbstractThe indica rice Xieqingzao eB1 (XQZ eB1), a mutant with an elongated uppermost internode, was produced by mutagenesis from elite maintainer line Xieqingzao B (XQZ B) using γ-radiation. Compared with the wild-type XQZ B, plant height and the length of the rice panicle neck, second internode and third internode from the panicle were elongated significantly in XQZ eB1. Genetic analysis indicated that the trait for uppermost internode elongation of XQZ eB1 is controlled by a single recessive gene eui1, which is allelic to that of IR50eui (derived from 76:4512). Further studies were carried out using the bulk segregated analysis approach. An F3 population, derived from the cross XQZ eB1×Aijiaonante, was used to map the mutant gene eui1. Two SSR makers, RM164 and AC9, were identified to link to eui1, their genetic distances to eui1 being 18.4 and 7.9 cM, respectively. The eui1 gene was also mapped to the long arm of chromosome 5 in this research.

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