Hyperbaric Oxygen Treatment in a Diabetic Rat Model Is Associated with a Decrease in Blood Glucose, Regression of Organ Damage and Improvement in Wound Healing

Background: Type 2 Diabetes Mellitus (T2DM) is a world-wide metabolic disease with no cure from drugs and treatment. In China, The Traditional Chinese Medicine (TCM) herbal formulations have been used to treat T2DM for centuries. Methods: In this study, we proposed a formula called ShenQi Compound (SQC), which has been used in clinical therapeutics in China for several years. We evaluated the effect of SQC in a spontaneous diabetic rat model (GK rats) by detecting a series of blood indicators and performing histological observations. Meanwhile, the gene microarray and RT-qPCR experiments were used to explore the molecular mechanism of SQC treatment. In addition, western medicine, sitagliptin was employed as a comparison. Results: The results indicated that SQC and sitagliptin could effectively improve the serum lipid (blood Total Cholesterol (TC) and blood Triglycerides (TG)), hormone levels (serum insulin (INS), Glucagon (GC) and Glucagon-Like Peptide-1 (GLP-1)), alleviated the inflammatory response (hypersensitive C-Reactive Protein (hsCRP)), blood glucose fluctuation (Mean Blood Glucose (MBG), standard deviation of blood glucose (SDBG) and Largest Amplitude of plasma Glucose Excursions (LAGE)), pancreatic tissue damage and vascular injury for T2DM. Compared with sitagliptin, SQC achieved a better effect on blood glucose fluctuation (p<0.01). Meanwhile, the gene microarray and RT-qPCR experiments indicated that SQC and sitagliptin may improve the T2DM through affecting the biological functions related to apoptosis and circadian rhythm. Moreover, SQC might be able to influence the mTOR signaling pathway by regulating Pik3r1, Ddit4 expression. Conclusion: All these results indicate that SQC is an effective therapeutic drug on T2DM. Notably, SQC presents an obvious blood glucose fluctuation-preventing ability, which might be derived from the regulation of the mTOR signaling pathway.

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Hyperbaric oxygen treatment reverses radiation induced pro-fibrotic and oxidative stress responses in a rat model

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2016.12.036 ◽

2017 ◽

Vol 103 ◽

pp. 248-255 ◽

Cited By ~ 12

Author(s):

N. Oscarsson ◽

L. Ny ◽

J. Mölne ◽

F. Lind ◽

S.-E. Ricksten ◽

...

Keyword(s):

Oxidative Stress ◽

Hyperbaric Oxygen ◽

Rat Model ◽

Stress Responses ◽

Hyperbaric Oxygen Treatment ◽

Oxygen Treatment ◽

Radiation Induced ◽

Oxidative Stress Responses ◽

And Oxidative Stress

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Effect ofCostus spiralis(Jacq.)RoscoeLeaves, Methanolic Extract and Guaijaverin on Blood Glucose and Lipid Levels in a Type II Diabetic Rat Model

Chemistry & Biodiversity ◽

10.1002/cbdv.201800365 ◽

2019 ◽

Vol 16 (1) ◽

pp. e1800365

Author(s):

Regiane C. Duarte ◽

Silvia H. Taleb-Contini ◽

Paulo S. Pereira ◽

Camila F. Oliveira ◽

Carlos Eduardo S. Miranda ◽

...

Keyword(s):

Blood Glucose ◽

Rat Model ◽

Methanolic Extract ◽

Diabetic Rat ◽

Type Ii ◽

Lipid Levels ◽

Diabetic Rat Model

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Patient reported experience of blood glucose management when undergoing hyperbaric oxygen treatment

Collegian Journal of the Royal College of Nursing Australia ◽

10.1016/j.colegn.2018.11.004 ◽

2019 ◽

Vol 26 (4) ◽

pp. 428-434

Author(s):

Carol Baines ◽

Geraldine O’Rourke ◽

Charne Miller ◽

Karen Ford ◽

William McGuiness

Keyword(s):

Blood Glucose ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Treatment ◽

Oxygen Treatment ◽

Patient Reported ◽

Glucose Management

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Shenqi Jiangtang Granule Ameliorates Kidney Function by Inhibiting Apoptosis in a Diabetic Rat Model

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/3240618 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Qian Zhang ◽

Xinhua Xiao ◽

Jia Zheng ◽

Ming Li ◽

Miao Yu ◽

...

Keyword(s):

Blood Glucose ◽

Serum Creatinine ◽

Kidney Function ◽

Rat Model ◽

Urinary Albumin ◽

Diabetic Group ◽

Diabetic Rat ◽

Apoptosis Pathway ◽

A Genome ◽

Diabetic Rat Model

Diabetic nephropathy (DN) is a major microvascular complication of diabetes. In addition to moderating hyperglycemia, Shenqi Jiangtang Granule (SJG) had a beneficial effect on kidney function in a clinical trial. However, the mechanism involved remains unclear. This study was conducted to identify the underlying molecular mechanisms. A diabetic rat model was generated by using a high-fat diet and streptozotocin (STZ) injection. Then, rats were given SJG at dosages of 400 mg/kg/d or 800 mg/kg/d by gavage for 8 weeks. After 8 weeks of treatment, blood glucose, serum creatinine, blood urea nitrogen (BUN), and 24-h urinary albumin were measured. Histochemical staining and TdT-mediated dUTP nick-end labeling (TUNEL) assays were performed in kidney. Kidney genomic expression in the SJG-treated group and diabetic group was detected by using a genome expression microarray. We found that SJG treatment reduced blood glucose, serum creatinine, BUN, and 24-h urinary albumin and affected kidney histology. The gene array revealed that the expression of 99 genes increased and the expression of 91 genes decreased in the HSJG group, compared with those of in the diabetic group. Pathway and gene ontology analysis of the differentially expressed genes showed an enrichment of the apoptosis pathway. SJG treatment reduced TUNEL- and caspase-3-positive cells in diabetic kidneys. SJG upregulated Bcl-2 and regucalcin expressions and reduced casp3 and Apaf1 expressions in diabetic rats. Our results suggest that SJG exerts a renal protective effect through the inhibition of cell apoptosis in a diabetic rodent model.

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Composite sponges from sheep decellularized small intestinal submucosa for treatment of diabetic wounds

Journal of Biomaterials Applications ◽

10.1177/0885328220963897 ◽

2020 ◽

pp. 088532822096389

Author(s):

Gamze Kara Magden ◽

Cigdem Vural ◽

Busra Yaprak Bayrak ◽

Candan Yilmaz Ozdogan ◽

Halime Kenar

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Rat Model ◽

Small Intestinal Submucosa ◽

Diabetic Rat ◽

Control Group ◽

Significant Difference ◽

Small Intestinal ◽

Intestinal Submucosa ◽

Diabetic Rat Model

Despite the fast development of technology in the world, diabetic foot wounds cause deaths and massive economical losses. Diabetes comes first among the reasons of non traumatic foot amputations. To reduce the healing time of these fast progressing wounds, effective wound dressings are in high demand. In our study, sheep small intestinal submucosa (SIS) based biocompatible sponges were prepared after SIS decellularization and their wound healing potential was investigated on full thickness skin defects in a diabetic rat model. The decellularized SIS membranes had no cytotoxic effects on human fibroblasts and supported capillary formation by HUVECs in a fibroblast-HUVEC co-culture. Glutaraldehyde crosslinked sponges of three different compositions were prepared to test in a diabetic rat model: gelatin (GS), gelatin: hyaluronic acid (GS:HA) and gelatin: hyaluronic acid: SIS (GS:HA:SIS). The GS:HA:SIS sponges underwent a 24.8 ± 5.4% weight loss in a 7-day in vitro erosion test. All sponges had a similar Young’s modulus under compression but GS:HA:SIS had the highest (5.00 ± 0.04 kPa). Statistical analyses of histopathological results of a 12-day in vivo experiment revealed no significant difference among the control, GS, GS:HA, and GS:HA:SIS transplanted groups in terms of granulation tissue thickness, collagen deposition, capillary vessel formation, and foreign body reaction (P > 0.05). On the other hand, in the GS:HA:SIS transplanted group 80% of the animals had a complete epidermal regeneration and this was significantly different than the control group (30%, P < 0.05). Preclinical studies revealed that the ECM of sheep small intestinal submucosa can be used as an effective biomaterial in diabetic wound healing.

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