Exploration of the Effect and Mechanism of ShenQi Compound in a Spontaneous Diabetic Rat Model

2019 ◽  
Vol 19 (5) ◽  
pp. 622-631 ◽  
Author(s):  
Ya Liu ◽  
Jian Kang ◽  
Hong Gao ◽  
Xiyu Zhang ◽  
Jun Chao ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Signaling Pathway ◽  
Rat Model ◽  
Mtor Signaling ◽  
Diabetic Rat ◽  
Gene Microarray ◽  
Mtor Signaling Pathway ◽  
Glucose Fluctuation ◽  
Blood Glucose Fluctuation ◽  
Diabetic Rat Model

Background: Type 2 Diabetes Mellitus (T2DM) is a world-wide metabolic disease with no cure from drugs and treatment. In China, The Traditional Chinese Medicine (TCM) herbal formulations have been used to treat T2DM for centuries. Methods: In this study, we proposed a formula called ShenQi Compound (SQC), which has been used in clinical therapeutics in China for several years. We evaluated the effect of SQC in a spontaneous diabetic rat model (GK rats) by detecting a series of blood indicators and performing histological observations. Meanwhile, the gene microarray and RT-qPCR experiments were used to explore the molecular mechanism of SQC treatment. In addition, western medicine, sitagliptin was employed as a comparison. Results: The results indicated that SQC and sitagliptin could effectively improve the serum lipid (blood Total Cholesterol (TC) and blood Triglycerides (TG)), hormone levels (serum insulin (INS), Glucagon (GC) and Glucagon-Like Peptide-1 (GLP-1)), alleviated the inflammatory response (hypersensitive C-Reactive Protein (hsCRP)), blood glucose fluctuation (Mean Blood Glucose (MBG), standard deviation of blood glucose (SDBG) and Largest Amplitude of plasma Glucose Excursions (LAGE)), pancreatic tissue damage and vascular injury for T2DM. Compared with sitagliptin, SQC achieved a better effect on blood glucose fluctuation (p<0.01). Meanwhile, the gene microarray and RT-qPCR experiments indicated that SQC and sitagliptin may improve the T2DM through affecting the biological functions related to apoptosis and circadian rhythm. Moreover, SQC might be able to influence the mTOR signaling pathway by regulating Pik3r1, Ddit4 expression. Conclusion: All these results indicate that SQC is an effective therapeutic drug on T2DM. Notably, SQC presents an obvious blood glucose fluctuation-preventing ability, which might be derived from the regulation of the mTOR signaling pathway.

Effect ofCostus spiralis(Jacq.)RoscoeLeaves, Methanolic Extract and Guaijaverin on Blood Glucose and Lipid Levels in a Type II Diabetic Rat Model

2019 ◽  
Vol 16 (1) ◽  
pp. e1800365
Author(s):  
Regiane C. Duarte ◽  
Silvia H. Taleb-Contini ◽  
Paulo S. Pereira ◽  
Camila F. Oliveira ◽  
Carlos Eduardo S. Miranda ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Rat Model ◽  
Methanolic Extract ◽  
Diabetic Rat ◽  
Type Ii ◽  
Lipid Levels ◽  
Diabetic Rat Model

scholarly journals Dihydromyricetin promotes autophagy and attenuates renal interstitial fibrosis by regulating miR-155-5p/PTEN signaling in diabetic nephropathy

2019 ◽  
Author(s):  
Liming Guo ◽  
Kuibi Tan ◽  
Qun Luo ◽  
Xu Bai
Keyword(s):  
Diabetic Nephropathy ◽  
Signaling Pathway ◽  
Rat Model ◽  
Interstitial Fibrosis ◽  
Mtor Signaling ◽  
Luciferase Assay ◽  
Luciferase Reporter ◽  
Mtor Signaling Pathway ◽  
Renal Interstitial Fibrosis ◽  
Gene Assay

Diabetic nephropathy (DN) is the most common complication of diabetes and is prone to kidney failure. Dihydromyricetin (DHM) has been reported to have a variety of pharmacological activities. This study aims to explore the effect of DHM on DN and the underlying molecular mechanism. An in vivo DN rat model was established. The degree of renal interstitial fibrosis (RIF) was detected by hematoxylin-eosin (HE) staining, Masson's trichrome staining, and immunohistochemistry (IHC). In vitro, NRK-52E cells were divided into four groups: normal glucose (NG), high glucose (HG), HG+DHM, and HG+rapamycin (autophagy inhibitor). The levels of autophagy- and fibrosis-related proteins were analyzed by western blotting. The expression of miR-155-5p and phosphatase and tensin homolog deleted on chromosome ten (PTEN) and their relationship were assessed by quantitative reverse transcription (qRT)-PCR and dual luciferase reporter gene assay. Our results showed that RIF was increased in DN rat model and in HG-induced NRK-52E cells. DHM treatment attenuated the increased RIF and also increased autophagy. MiR-155-5p expression was increased, while PTEN expression was decreased in DN rat and cell model, and DHM reversed both effects. Dual luciferase assay showed that PTEN was the target gene of miR-155-5p. DHM inhibited HG-induced fibrosis and promoted autophagy by inhibiting miR-155-5p expression in NRK-52E cells. In addition, DHM promoted autophagy by inhibiting the PI3K/AKT/mTOR signaling pathway. In conclusion, DHM promotes autophagy and attenuates RIF by regulating the miR-155-5p/PTEN signaling and PI3K/AKT/mTOR signaling pathway in DN.

scholarly journals Shenqi Jiangtang Granule Ameliorates Kidney Function by Inhibiting Apoptosis in a Diabetic Rat Model

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Qian Zhang ◽  
Xinhua Xiao ◽  
Jia Zheng ◽  
Ming Li ◽  
Miao Yu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Serum Creatinine ◽  
Kidney Function ◽  
Rat Model ◽  
Urinary Albumin ◽  
Diabetic Group ◽  
Diabetic Rat ◽  
Apoptosis Pathway ◽  
A Genome ◽  
Diabetic Rat Model

Diabetic nephropathy (DN) is a major microvascular complication of diabetes. In addition to moderating hyperglycemia, Shenqi Jiangtang Granule (SJG) had a beneficial effect on kidney function in a clinical trial. However, the mechanism involved remains unclear. This study was conducted to identify the underlying molecular mechanisms. A diabetic rat model was generated by using a high-fat diet and streptozotocin (STZ) injection. Then, rats were given SJG at dosages of 400 mg/kg/d or 800 mg/kg/d by gavage for 8 weeks. After 8 weeks of treatment, blood glucose, serum creatinine, blood urea nitrogen (BUN), and 24-h urinary albumin were measured. Histochemical staining and TdT-mediated dUTP nick-end labeling (TUNEL) assays were performed in kidney. Kidney genomic expression in the SJG-treated group and diabetic group was detected by using a genome expression microarray. We found that SJG treatment reduced blood glucose, serum creatinine, BUN, and 24-h urinary albumin and affected kidney histology. The gene array revealed that the expression of 99 genes increased and the expression of 91 genes decreased in the HSJG group, compared with those of in the diabetic group. Pathway and gene ontology analysis of the differentially expressed genes showed an enrichment of the apoptosis pathway. SJG treatment reduced TUNEL- and caspase-3-positive cells in diabetic kidneys. SJG upregulated Bcl-2 and regucalcin expressions and reduced casp3 and Apaf1 expressions in diabetic rats. Our results suggest that SJG exerts a renal protective effect through the inhibition of cell apoptosis in a diabetic rodent model.

scholarly journals Involvement of the NF-κB signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model

2016 ◽  
Vol 4 (5) ◽  
pp. 628-634 ◽  
Author(s):  
SHUJUAN QIU ◽  
GUILING SUN ◽  
YUNXIA ZHANG ◽  
XIANGLING LI ◽  
RONG WANG
Keyword(s):  
Signaling Pathway ◽  
Rat Model ◽  
Diabetic Rat ◽  
Diabetic Rat Model ◽  
Type 2 Diabetic

Effect of Curcumin on the Level of Blood Lipid and Blood Glucose in Diabetic Rat Model

2014 ◽  
Vol 1051 ◽  
pp. 419-422
Author(s):  
Ming San Miao ◽  
Lin Guo ◽  
Shuo Tian ◽  
Tan Wang
Keyword(s):  
Blood Glucose ◽  
Rat Model ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Lipoprotein Cholesterol ◽  
Diabetic Rat ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetic Rat Model

Objective: To investigate the effects of curcumin on blood lipid and blood glucose levels and its mechanism in diabetic rat model. Method: After streptozotocin (STZ) induced diabetic rat model, large, medium and small doses of curcumin group were partly given curcumin solution 400,200,100mg·kg-1, administered once a day, continuously 30 days. In 30 th day, determine blood glucose (BG) value, after the last injection , determine the serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) levels. Results: Compared with the model group rats, each dose of curcumin group rats’ BG, TC, TG and LDL-C levels were significantly decreased, HDL-C levels was increased significantly. Conclusion: Curcumin has a certain impact on blood lipids and blood glucose in diabetic rat model.

Blood glucose fluctuation accelerates renal injury involved to inhibit the AKT signaling pathway in diabetic rats

Endocrine ◽  
2016 ◽  
Vol 53 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Changjiang Ying ◽  
Xiaoyan Zhou ◽  
Zhenzhen Chang ◽  
Hongwei Ling ◽  
Xingbo Cheng ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Signaling Pathway ◽  
Renal Injury ◽  
Akt Signaling ◽  
Diabetic Rats ◽  
Akt Signaling Pathway ◽  
Glucose Fluctuation ◽  
Blood Glucose Fluctuation

scholarly journals Investigation of the Effects and Mechanisms of Mai Tong Formula on Lower Limb Macroangiopathy in a Spontaneous Diabetic Rat Model

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Guangming Gong ◽  
Haipo Yuan ◽  
Ya Liu ◽  
Luguang Qi
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Signaling Pathway ◽  
Rat Model ◽  
Lower Limb ◽  
Smooth Muscle Contraction ◽  
Diabetic Rat ◽  
Ampk Signaling ◽  
Vascular Smooth Muscle Contraction ◽  
Diabetic Rat Model

A new Chinese herbal formula called Mai Tong Formulae (MTF) has recently been used to treat lower limb macroangiopathy in type 2 diabetes mellitus (T2DM) patients. In this study, we investigated the effect of MTF on lower limb macroangiopathy in a spontaneous diabetic rat model (GK rats). We found that MTF treatment significantly reduced serum fasting blood glucose (FBG), triglycerides (TG), total cholesterol (TC), IL6, and VEGF and increased serum insulin in this model. Histological and ultrastructural observations showed that MTF treatment significantly reduced vascular endothelial cell shedding and improved endothelium injuries. We further detect proteome alteration following MTF treatment. 25 differential proteins (DPs) abnormally expressed in GK rats were normalized by MTF treatment. These DPs significantly are enriched in biological processes and pathways that regulate muscle contraction and cGMP-PKG signaling pathway and so on. Additional protein-protein interaction (PPI) network analyses of the DPs showed that Fasn and Prkar2a are involved in the AMPK signaling pathway, and Gnas, Myh11, and Myh6 are involved in vascular smooth muscle contraction; these 5 DPs were validated by Western blotting. These results indicate that MTF treatment effectively treats lower limb macroangiopathy by regulating key proteins involved in AMPK signaling pathway and vascular smooth muscle contraction.

Astaxanthin mediated regulation of VEGF through HIF1α and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model

2021 ◽  
pp. 108555
Author(s):  
Rajasekar Janani ◽  
Rani Elavarasan Anitha ◽  
Madan Kumar Perumal ◽  
Peethambaran Divya ◽  
Vallikannan Baskaran
Keyword(s):  
Signaling Pathway ◽  
Rat Model ◽  
Diabetic Rat ◽  
Diabetic Rat Model
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