scholarly journals Phenol-Oxidizing Enzyme Expression in Lentinula edodes by the Addition of Sawdust Extract, Aromatic Compounds, or Copper in Liquid Culture Media

2013 ◽  
Vol 18 (3) ◽  
pp. 143-149
Author(s):  
EIJI TANESAKA ◽  
HIRONORI TAKEDA ◽  
MOTONOBU YOSHIDA
Keyword(s):  
Aromatic Compounds ◽  
Liquid Culture ◽  
Lentinula Edodes ◽  
Culture Media ◽  
Enzyme Expression

scholarly journals Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 20
Author(s):  
Luigi De Grossi ◽  
Davide Santori ◽  
Antonino Barone ◽  
Silvia Abbruzzese ◽  
Matteo Ricchi ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Liquid Culture ◽  
Culture Media ◽  
Small Ruminants ◽  
Culture Method ◽  
Mycobacterium Avium Subsp ◽  
Type I ◽  
Culture Methods ◽  
Specific Pcr ◽  
Mycobacterium Avium Subsp Paratuberculosis

Paratuberculosis is a chronic disease of ruminants caused by Mycobacterium avium subsp. Paratuberculosis (MAP). Since isolation of MAP type I (S) is rarely reported in Italy, our research was aimed at isolating, by an inexpensive liquid culture manual method, this type of MAP isolates. At first, we used an ELISA to point out to serologically positive samples from five flocks. Secondly, we used a fecal direct IS900-qPCR on the ELISA positive samples, in order to detect shedder animals. Feces from IS900-qPCR positive samples were inoculated in solid and liquid culture media. IS900-qPCR was further used to test the growth of MAP isolates in liquid medium, which were further confirmed by f57-qPCR and submitted to typing by specific PCR in order to identify the MAP type. Twenty-eight samples (24 fecal and four tissutal samples) were processed by culture methods, resulting in the isolation of six type I MAP field isolates. Notably, no isolates were recovered by solid media, underlining the utility of this liquid method. Few data about this type of MAP are currently available in Italy, and further analyses should be carried out in order to study the origin and epidemiology of type I strains circulating in Italy.

On the cultivation of spir. pallida on liquid culture media

1926 ◽  
Vol 22 (1) ◽  
pp. 19-22
Author(s):  
V. M. Aristovskiy ◽  
R. R. Gel'ttser
Keyword(s):  
Liquid Culture ◽  
Culture Media ◽  
Nutrient Media ◽  
Reliable Methods

In recent years, great strides have been made on the cultivation of spirochetes, and if relatively simple and completely reliable methods of growing them in vitro on both solid and liquid nutrient media have been developed for a number of pathogenic and non-pathogenic spirochetes, then on the issue of With the cultivation of the pale spirochete, we are moving forward very slowly, and the successes achieved recently in this area must be recognized as very modest.

Production and characterization of biomass and exopolysaccharides obtained in submerged culture under different initial pHs used in the cultivation of Colletotrichum gloeosporioides and Rhizopus stolonifer

2020 ◽  
pp. 628
Author(s):  
Juan Diego Valenzuela Cobos ◽  
René Oscar Rodríguez-Grimón ◽  
Ana Grijalva-Endara ◽  
Raúl Marcillo-Vallejo ◽  
Onay Adonys Mercader-Camejo
Keyword(s):  
Yeast Extract ◽  
Submerged Culture ◽  
Liquid Culture ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Mycelial Biomass ◽  
Rhizopus Stolonifer

Colletotrichum gloeosporioides (GC003) and Rhizopus stolonifer (RS001) were cultivated in two different liquid culture media: LC1 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 2 g L-1) and LC2 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 10 g L-1) for the production of mycelial biomass and exopolysaccharides (EPS). By using the liquid culture (LC2) under pH of 4.5 presented the highest biomass content (15.73 g L-1) in the propagation of Rhizopus stolonifer. The highest production of exopolysaccharides (1.74 g L-1) was obtained by the liquid culture (LC2) under pH of 4.5 in the cultivation of Colletotrichum gloeosporioides. The results presented that the production of biomass and exopolysaccharides (EPS) is directly related with the pHs values and the strain used in the cultivation.

UTILIZATION OF MILK AND ANIMAL FAT BY MICROORGANISMS – POTENTIAL PRODUCERS OF LIPASES AND ESTERASES

2020 ◽  
pp. 44-45
Author(s):  
D. Ivasenko ◽  
P. Bukhtiyarova ◽  
D. Antsiferov ◽  
Y. Frank
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Acid Composition ◽  
Liquid Culture ◽  
Culture Media ◽  
Bacterial Strains ◽  
Animal Fat ◽  
Pure Cultures

Analysis of fatty acid composition in liquid culture media after lipophilic bacterial strains cultivation was carried out. Pure cultures were earlierisolated from fat-containing wastes and cultivated on media with diverse fat sources. It was shown that microorganisms hydrolyze animal and milk fats to free fatty acids.

Invitro Study of Carbon and Nitrogen Metabolism of Principal Fungi Associated with Fomesconnatus in Sugar Maple Acersaccharum

1973 ◽  
Vol 3 (2) ◽  
pp. 319-322 ◽  
Author(s):  
Terry A. Tattar ◽  
A. E. Rich
Keyword(s):  
Cell Walls ◽  
Liquid Culture ◽  
Sugar Maple ◽  
Narrow Band ◽  
Culture Media ◽  
Xylem Sap ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism ◽  
Successful Colonization

Isolates of Phialophoramelinii and Acrostaphylus sp. from discolored tissue, Trichodermaviride and Mortierella sp. from decayed tissue, and Fomesconnatus from a narrow band of discolored tissue at the border of decayed and discolored tissue, of sugar maple (Acersaccharum) were grown in liquid culture media containing sources of carbon and nitrogen found in tissue of sugar maple. These compounds included the carbohydrates of wood and their component monosaccharides and translocation compounds from xylem sap. Growth was measured as oven-dried weight of mycelium. All fungi utilized the carbohydrate and nitrogen sources, except Mortierella sp. which did not utilize cellulose or xylose. Only P. melinii utilized substantially gallic acid. The degradation of cell walls in living trees may occur both in discolored and decayed tissue and may be caused by nonhyme-nomycetous and hymenomycetous fungi. Selective utilization of host components by some of these may enable successful colonization of wounds and initiation of the processes of discoloration and decay.

A Wharton's Jelly Mesenchymal Stromal Cell Derived 3D Osteogenic Niche Allows for Cord Blood Stem Cell Expansion Using Cytokine-Free Culture Media

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4832-4832
Author(s):  
Omar S. Aljitawi ◽  
Peggy Keefe ◽  
Christianna Henderson ◽  
Salem Akel ◽  
Sunil Abhyankar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Liquid Culture ◽  
Conflicts Of Interest ◽  
Culture Media ◽  
Ex Vivo ◽  
Culture Conditions ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Free Media

Abstract Abstract 4832 Introduction: In prior experiments, a 3 dimensional (3D) osteogenic niche was developed using Wharton's jelly mesenchymal stromal cells (WJMSC) that allowed for successful attachment of CD34 + umbilical cord blood (UCB) stem cells. The 3D osteogenic scaffold resulted in 10 times expansion of total nucleated cells (TNCs), however, most of the expanded cells were CD34 negative possibly secondary to their differentiation attributed to the cytokine-rich media. Accordingly, it was proposed to use a cytokine-free media in expansion of CD34+UCB using the same 3D osteogenic structure. Aims: To assess the ex vivo expansion of CD34+UCB stem cells in a 3D osteogenic niche using cytokine-free culture media. The expansion in 3D conditions was compared to 2D and liquid-culture conditions. Methods: CD34+ selected UCB stem cells were expanded in 3D osteogenic scaffold using cytokine-free media for 2 weeks. In 2D conditions, CD34+ UCB stem cells were expanded over a monolayer of osteogenic differentiated WJMSCs using the same cytokine-free media. In liquid culture conditions, the CD34+ UCB stem cells were expanded in culture flask. Pre- and post-expanded TNC was determined and post expansion CD34 expression was assessed using flow cytometry. Colony forming unit (CFU) assays were used to compare the expanded population in the three culture conditions. Results: TNCs were expanded 26X in 3D, 265X in 2D, and only 2X in liquid culture conditions. CD34 expressing cells increased 2.4X in 3D, 10X in 2D, and decreased in liquid cultures. Relative to 3D, CFU expansion in 2D was increased 2.6X. In 3D, CFUs were almost exclusively CFU-GM (>93%), compared to 2D (74%). Absence of CFU-E was noticed in the expanded population that remained attached to the 3D osteogenic scaffold. On the other hand, low CFU-E percentage was noticed in the population of expanded cells that dettached from the 3D osteogenic scaffold. Conclusions: The experimental conditions lead to a modest but significant expansion of CD34+UCB stem cells in 3D as well as 2D conditions. Though the majority of the expanded population did not express CD34, they retained their colonogenic potential. Contrary to the expanded cells that detached from the 3D osteogenic scaffold, the cells that remained attached did not form CFU-E. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Micropropagation of Bacopa monnieri using Cyanobacterial Liquid Medium

1970 ◽  
Vol 20 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Meenakshi Banerjee ◽  
Priyanka Modi
Keyword(s):  
Plant Tissue ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Axillary Node ◽  
Bacopa Monnieri ◽  
Shoot Initiation ◽  
Aulosira Fertilissima

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots.  Rooted plantlets were successfully transferred to the field, after acclimation in the net house.   Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation   D.O.I. 10.3329/ptcb.v20i2.6917   Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)

Sign in / Sign up

Export Citation Format

Share Document