Genome wide analysis of  NAC transcription factors and their expression pattern during high temperature and drought stress in groundnut

Mungbean (Vigna radiata L.) is a valuable legume crop grown in tropical and subtropical areas of Asia. Drought is one of the major factors hindering its growth globally. APETALA2/ethylene-responsive element factor binding proteins (AP2/ERF) are an important family of plant-specific transcription factors (TFs) involved in drought-stress tolerance. We identified 71 AP2/ERF TFs in the mungbean genome by using bioinformatics tools and classified them into subfamilies: AP2 (16 members), ERF (22), RAV (2), DREB (30) and soloist (other proteins with no domain, 1). Members of DREB play a critical role in drought-stress tolerance. Ten-day-old mungbean plants cv. AZRI-06 were exposed to drought stress by complete withholding of water for 7 days. Root samples were collected from control and drought-stressed plants, and the expression pattern of 30 identified VrDREB genes was determined by qPCR. Most VrDREB genes exhibited differential expression in response to drought. Five genes (VrDREB5, VrDREB12, VrDREB13, VrDREB22, VrDREB30) were highly expressed under drought stress and might be considered excellent candidates for further functional analysis and for improvement of mungbean drought tolerance.

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Peer Review #3 of "Genome-wide analysis of transcription factors related to anthocyanin biosynthesis in carmine radish (Raphanus sativus L.) fleshy roots (v0.1)"

10.7287/peerj.8041v0.1/reviews/3 ◽

2019 ◽

Keyword(s):

Transcription Factors ◽

Peer Review ◽

Raphanus Sativus ◽

Anthocyanin Biosynthesis ◽

Genome Wide Analysis ◽

Genome Wide ◽

Raphanus Sativus L

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Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v4i4.279-290.580 ◽

2016 ◽

Vol 4 (4) ◽

pp. 279 ◽

Cited By ~ 2

Author(s):

Shaiq Sultan ◽

Muhammad Amjid Ali ◽

Rana Muhammad Atif ◽

Farrukh Azeem ◽

Habibullah Nadeem ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factors ◽

Chromosomal Localization ◽

Evolutionary Relationship ◽

Wrky Transcription Factors ◽

Specific Sequence ◽

Multiple Sequence ◽

Genome Wide Analysis ◽

Functional Homology ◽

Genome Wide

WRKY transcription factors are a class of DNA-binding proteins that bind with a specific sequence C/TTGACT/C known as W-Box found in promoters of genes which are regulated by these WRKYs. From previous studies, 43 different stress responsive WRKY transcription factors in Arabidopsis thaliana, identified and then categorized in three groups viz., abiotic, biotic and both of these stresses. A comprehensive genome wide analysis including chromosomal localization, gene structure analysis, multiple sequence alignment, phylogenetic analysis and promoter analysis of these WRKY genes was carried out in this study to determine the functional homology in Arabidopsis. This analysis led to the classification of these WRKY family members into 3 major groups and subgroups and showed evolutionary relationship among these groups on the base of their functional WRKY domain, chromosomal localization and intron/exon structure. The proposed groups of these stress responsive WRKY genes and annotation based on their position on chromosomes can also be explored to determine their functional homology in other plant species in relation to different stresses. The result of the present study provides indispensable genomic information for the stress responsive WRKY transcription factors in Arabidopsis and will pave the way to explain the precise role of various AtWRKYs in plant growth and development under stressed conditions.

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