scholarly journals Enumeration of total and Feacal coliform bacteria of some borehole water in Gombe, Gombe State, Nigeria

2020 ◽  
Vol 12 (1) ◽  
pp. 221-226
Author(s):  
Nuru Adamu Garkuwa ◽  
Sa’adatu Said Mustapha ◽  
Sani Aliyu Ibrahim ◽  
Muhammad Yusha’u ◽  
Mansur Abdulrasheed ◽  
...  

Inadequate provision of potable drinking water in relation to teaming population is forcing most of the urban communities to drink untreated water obtained from traditional boreholes. Hence, the need to assess the portability of drinking water. In this research, boreholes water samples collected within Gombe city were investigated bacteriologically using multiple tube fermentation and pour plate methods to examine feacal coliform and coliform bacterial counts respectively; bacterial isolation was conducted using standard culture methods.  Identification of the bacterial Isolates were carried out by macroscopic, microscopic and biochemical examinations. Total coliform bacteria ranges from <3 to 11 MPN/100 ml and 1 to 3 MPN/100ml for faecal coliform. The borehole water samples from studied areas had high counts of faecal coliforms (Escherichia coli), and other pathogens like Entrobacter and Salmonella. The findings showed that all the borehole water analysed were not suitable for human consumption and may pose a serious threat to the health of consumers and therefore, there is need for treatment of these borehole waters by the borehole proprietors and also by simple treatment methods such as boiling, filtration before drinking and agitation by the consumers.    Keywords: Borehole water, Quality, Isolation, Identification, Coliform bacteria

2012 ◽  
Vol 9 (2) ◽  
pp. 608-614 ◽  
Author(s):  
Emrah Caylak ◽  
Mustafa Tokar

Safe and good quality drinking water is very important for human health. Water provides some heavy metals, such as As, B, Cd, Cn, Cr, Cu, F, Cl, Hg, Ni, Pb, Se, and Sn. These are undesirable substances dangerous to human health and cause chronic diseases and various cancers. Coliform bacteria are a commonly used bacterial indicator of sanitary quality of water.E. coli, total coliform and enterococci have been used as indicators of possible sewage contamination might pose a health risk. In this study, water samples were taken from thirty different waterworks in Cankiri, Turkey and metallic and microbial contaminants were investigated. In the drinking tap water samples have been found at concentrations exceeding the Turkish regulations (As and free Cl) and the WHO guidelines (As and B). Our results also indicate that significant microbiological contaminations were found above the guidelines for drinking waters given by the WHO, because of the fact that in the distribution of water is not appropriate.


2014 ◽  
Vol 13 (2) ◽  
pp. 340-352 ◽  
Author(s):  
Andrée F. Maheux ◽  
Vanessa Dion-Dupont ◽  
Sébastien Bouchard ◽  
Marc-Antoine Bisson ◽  
Michel G. Bergeron ◽  
...  

The MI agar, Colilert®, Chromocult coliform® agar, and DC with BCIG agar chromogenic culture-based methods used to assess microbiological quality of drinking water were compared in terms of their ubiquity, sensitivity, ease of use, growth of atypical colonies and affordability. For ubiquity, 129 total coliform (representing 76 species) and 19 Escherichia coli strains were tested. Then, 635 1-L well water samples were divided into 100 mL subsamples for testing by all four methods. Test results showed that 70.5, 52.7, 36.4, and 23.3% of the non-E. coli total coliform strains and 94.7, 94.7, 89.5, and 89.5% of the 19 E. coli strains yielded a positive signal with the four methods, respectively. They also yielded a total coliform positive signal for 66.5, 51.7, 64.9, and 55.0% and an E. coli positive signal for 16.1, 14.8, 17.3, and 13.4% of the 635 well water samples tested, respectively. Results showed that Colilert® is the most expensive method tested in terms of reactants, yet it is the easiest to use. Large numbers of atypical colonies were also often observed on Chromocult coliform® and DC with BCIG, thereby challenging the target microorganism count. Thus, the MI agar method seems to be the best option for the assessment of drinking water quality.


2018 ◽  
Vol 5 ◽  
pp. 83-88 ◽  
Author(s):  
Sujan Maharjan ◽  
Tista Prasai Joshi ◽  
Sujen Man Shrestha

Objectives: In order to evaluate the quality assurance of drinking water in Kathmandu valley, this study analyzed selected physiochemical and microbial parameters of treated water samples and compared with Nepal Drinking Water Quality Standards (NDWQS). Methods: Treated water samples were collected from all over the Kathmandu valley and analyzed in terms of physicochemical and microbiological parameters over the period of one year from July 2017 to July 2018. The physio-chemical parameters of water samples were performed according to standard methods for the examination of water and wastewater. The total coliforms were enumerated by standard membrane filtration technique. Results: We report that microbiological aspect of treated water was the major problem as 66% of the water samples crossed the guideline value for total coliform count. Above 92% of jar water samples, 77% of tanker water samples and 69% of filtered water samples had the total coliform count exceeding the NDWQS. Moreover, 20% of bottled water was contaminated by coliform bacteria. Iron and ammonia content were found to be higher than the guideline values in 16% and 21% of the total treated water samples respectively. Analyzing the types of treated water samples showed that 35% and 15% of tanker water samples had higher ammonia and iron content respectively, and the same parameters were higher in 23% and 19% in the filtered water samples respectively than the standard criteria recommended by NDWQS. Conclusion: The treated water samples exceed the standard values set by NDWQS and hence had poor quality. The presence of faecal pollution indicating coliform bacteria was the key problem for treated drinking water of Kathmandu valley. Therefore, monitoring and proper treatment of water should be conducted to prevent dissemination of waterborne diseases.


2015 ◽  
Vol 15 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Neel Kamal Koju ◽  
Tista Prasai ◽  
Sujen Man Shrestha ◽  
Prakash Raut

This research was conducted to analyze drinking water quality of Kathmandu valley. Total 969 water samples (392 from dug wells, 287 from deep boring, 218 from treated water, 46 from tap and 26 from other water sources) received from different places of Kathmandu, Lalitpur and Bhaktapur districts during March 2012 to March 2013. These samples were analyzed for the determination of physical (pH, temperature, conductivity, turbidity), chemical (hardness, chloride, iron, arsenic, ammonia, nitrate) and microbiological (total Coliform) parameters. It was found that the temperature and nitrate were within the WHO standards while pH, conductivity, turbidity, chloride, iron, arsenic, ammonia, and Coliform bacteria exceeded the WHO standard guideline. In ground water ( well and Boring), pH, conductivity, hardness, turbidity, iron, arsenic, chloride, ammonia and total Coliform count crossed WHO standards as 5%, 2%, 0.8%, 36%, 51%, 0.1%, 2%, 11% and 86 % respectively. Hardness was within the standard in both  reated and tap water samples. Compared to treated water, pH, arsenic and chloride were within the standard in tap water. The common problematic parameters of different sources of drinking were turbidity, iron, ammonia and conductivity. Coliform bacteria were found in 36% samples of treated water whereas 80% tap water samples were contaminated from Coliform bacteria.DOI: http://dx.doi.org/10.3126/njst.v15i1.12027Nepal Journal of Science and TechnologyVol. 15, No.1 (2014) 115-120


2018 ◽  
Vol 1 (4) ◽  
pp. 241-247
Author(s):  
M U Iduh ◽  
T I Spencer ◽  
K Mohammed ◽  
K M Garba ◽  
F O Ashcroft ◽  
...  

A total of 50 water samples comprising; 20 well water, 15 sachet water, 10 borehole water and 5 river water samples, were obtained from Wamakko, Dange-shuni and Wurno local government areas in Sokoto Central, Sokoto West and Sokoto East, Nigeria respectively between March and May 2015. They were analyzed using membrane filtration and pour plate techniques for faecal coliforms count and total coliform count respectively. The total coliform count for all the sources ranged between 0 and 3.28 × 102 with a total mean of 1.11 × 10,2 that of faecal coliform count was found to range between 0 and 92 with a mean total count of 12.58. Among the bacteria isolated and identified, Escherichia coli had the highest total prevalence of 233 (40.31%) and occurring in all the water samples. Klebsiella spp had 99 (17.13%) occurring in all the sources, Salmonella spp 43 (7.44%) occurring in all the sources except for borehole, Pseudomonas species 88 (15.22%) occurring in all the sources, Staphylococcus aureus with 115 (19.90%) occurring in all the sources except for borehole. However, the pHs for all the samples were found to be within the range of 6.51 to 8.0 which are within WHO acceptable range of 6.0 to 8.5. From this study, it is obvious that the water sources from the areas under study are bacteriologically unfit for drinking purposes. These samples with high total coliform count are of concern and calls for urgent and stringent measures aimed at ensuring a safer drinking water for the populace.


Author(s):  
Priscilla Alexander ◽  
I. B. Bwatanglang ◽  
Juth Daniel

Physicochemical and Bacteriological Parameters of Borehole and Hand dug well water of Michika town in Michika Local Government Area of Adamawa State, Nigeria and environs were assessed to determine their suitability or otherwise for drinking and domestic purposes. Ten (10) water samples, five each from boreholes and hand-dug wells, from five selected areas in Michika town, were collected during the months of January and February 2018. The water samples which are extensively used for drinking and other domestic purposes, were randomly collected and the results were compared with WHO and NAFDAC standards guidelines for drinking water. These samples were analyzed for their physicochemical characteristics (pH, temperature, electrical conductivity, TDS, Turbidity, hardness), heavy metals, Escherichia coli (E. coli) and coliform counts. The results of the investigation revealed that the physicochemical and bacteriological parameters falls within the maximum permissible limits of NAFDAC and WHO guidelines for drinking water. The physicochemical concentrations were higher in borehole water than in hand dug well water; Lead and Cadmium were not detected in all the samples. All the water samples were free from feacal contamination except in Barikin Dlaka hand dug well which contained 0.05 ± 0.001 MPN/100 ml total coliform count which is below the WHO/NAFDAC maximum permissible levels. The suitability of water for domestic and drinking purposes indicated that the water samples were within the standards prescribed for potable water. However, there is need for routine checks to ascertain the suitability or otherwise of these water sources so as to forestall outbreak of water born diseases.


Author(s):  
Suntorn Sudsandee ◽  
Krailak Fakkaew ◽  
Vivat Keawdounglek ◽  
Pussadee Laor ◽  
Suwalee Worakhunpiset ◽  
...  

Hill tribes are a group of people who live in remote areas in northern Thailand. They typically use untreated water for drinking, that can lead several health problems. The six main hill tribes—Akha, Hmong, Karen, Lahu, Lisu, and Yao—were selected for the study. A validated questionnaire was used for data collection. Water samples were collected from the selected villages and tested for the quality at Mae Fah Luang University, Thailand. Results: the major sources of drinking water were mountain water supplies (74.3%), and commercial bottled water (21.4%). Easy access, sufficiency for the whole year, and food-drug administration sign labeled were the criteria used for selecting sources of drinking water. Colorless and safety were also used as a selection criteria for their drinking water in some tribes. Lisu, Karen, and Hmong treated their drinking water by boiling, while Akha and Lahu stored the water in certain containers to allow particle settling before drinking water without treatment. 42.0% of the water samples had a turbidity values <5 NTU, and total coliform and fecal coliform bacteria were detected in 100.0% of the samples. To prevent water-borne diseases among the hill tribe people, appropriate water treatments such as boiling, filtration, and disinfection are recommended.


2015 ◽  
Vol 16 (2) ◽  
pp. 378-387 ◽  
Author(s):  
Elizabeth O. Oloruntoba ◽  
Toyin F. Babalola ◽  
Oyewale M. Morakinyo ◽  
Adejumo Mumuni

This study assessed the effectiveness of improved storage containers on household drinking water quality in four low-income urban communities in Ibadan, Nigeria. Three hundred randomly selected respondents were interviewed, while 44 households were selected and randomly assigned to four improved container treatment groups: Covered Buckets with Taps (CBT), Covered Buckets without Taps (CB), Covered Kegs with Taps (CKT) and Covered Kegs without Taps (CK). Water samples from springs, regular storage containers (RSC), and improved containers were analysed for total coliform (TC), total viable bacteria (TVB) and Escherichia coli for 2 weeks. About 96% reported using the same containers for cooking and drinking water, while only 23.3% used a form of water treatment. TC count for RSC and CB exceeded the recommended limit. Only 3 (6.8%) of the samples from RSC contained E. coli. A statistically significant difference was observed between the mean TC counts of samples from the improved containers. Percentage reduction in TC count from RSC, and the improved containers (CB, CBT, CK and CKT) were 25.4%, 37.3%, 45.0%, 56.8% and 53.8% respectively. Bacillus, Staphylococcus, Klebsiella, Proteus and Pseudomonas were isolated from the water samples. CK produced the best result. Hygiene education on use of appropriate storage containers for drinking water is recommended at the household level.


Author(s):  
Kanij Fatema Nishan ◽  
Nilufa Yeasmin ◽  
Urmi Rani Devi ◽  
Sumiya Akter ◽  
Md. Abu Bakar ◽  
...  

Chattogram is the second most populated city in Bangladesh. This port city faces a serious threat mainly due to the lack of safe drinking water. This study was conducted for determining drinking water quality of groundwater sources in Chattogram city. The study was performed in the BCSIR laboratory, Chattogram. It was carried out for a period of six months from 1st July, 2018 to 31th December, 2018. Total six water samples were collected from three different locations (Baluchora, C&B colony and Khulshi area). Each sampling location consists of two separate sampling points. Physicochemical parameters of the collected samples like Temperature, pH, Electrical Conductivity (EC), Total dissolved solid (TDS), Hardness, Turbidity and concentration of Cl, As, Mn, Fe, Pb, Cr and Cd were examined. Microbial parameters like Total Coliform (TC) were also measured. All the analyzed parameters compared with BSTI and WHO drinking water quality standards to understand the overall ground water quality status of the study area. The results reveal that water samples in almost all locations were contaminated with microbial contamination and that the range of physico-chemical parameters was not adequate for consumption. Preliminary treatments like boiling, filtering etc are required before using groundwater for drinking and the necessary measures must be taken for a safe alternative source of drinking water.


Author(s):  
H. O. Stanley ◽  
C. N. Eze

A study about the bacteriological and physicochemical quality of borehole water at the University of Port Harcourt was carried out. Eight water samples was pooled from NUH Block B (Under graduate Hostel), Nelson Mandela Block B (Undergraduate Hostel), Intercontinental Hostel (Post Graduate Hostel), Donald Ekong Block C (Post graduate Hostel), Clinical Hostel, Staff quarters (Opposite Uniport Bottling plant), Dan Etete (Undergraduate Hostel) and Gambiama Staff quarters designated as Sample 1 to sample 8 respectively. Physical examination was conducted to as the sanitary and hygiene practices within the collection area. It was observed that the undergraduate hostels had the least sanitary practice. Total counts of heterotrophic bacteria count showed that the highest bacterial count was recorded from Sample 2 with bacterial count of 2.3x104 CFU/ml while the least bacterial count was recorded from sample 8 with bacterial count of 3.0x102 CFU/ml. The isolated bacterial species from the water sources were identified as Bacillus sp., Micrococcus sp., E. coli., Serratia sp., Staphylococcus sp., Enterobacter sp., Citrobacter sp. The presence of coliform bacteria in the sampled water source does not comply with the World Health Organization (WHO) standard for coliform bacteria of zero total coliform per 100 ml of water. The borehole water samples collected had pH values within 5.9-6.85 which does not comply with the WHO recommended range for drinking water standards which should fall between ≥7 to ≤9.2. Nitrate concentration as observed amongst the eight water samples was below the WHO standard of 50mg/l. The Total dissolved solutes was below 0.01 in all the tested waters samples. This study has revealed that borehole water from sampled sources within the University of Port Harcourt is not fit for human consumption without adequate treatment.


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