scholarly journals Antimicrobial activities of extracts from stem bark of Tagetes minuta

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2089-2094
Author(s):  
M.K. Pillai ◽  
L.I. Santi ◽  
S.B. Mekbib
Keyword(s):  
Escherichia Coli ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bark Extract ◽  
Bacterial Isolates ◽  
Tagetes Minuta ◽  
Food And Beverage ◽  
Stem Bark Extract

Tagetes minuta hexane stem bark extract (TMHESB), chloroform stem bark extract (TMCHSB), ethyl acetate stem bark extract (TMEASB) and methanolic stem bark extract (TMMESB) were evaluated for their antimicrobial activities using hole-plate diffusion method. Six bacterial isolates viz. Staphylococcus aureus, Listeria monocytogenes, Escherichia coli (wild), Escherichia coli (O157:H7), Pseudomonas aeruginosa and Serratia marcescens and two fungal isolates viz. Candida albicans and Penicillium digitatum were used for this study. The inhibition zones were found to be in the ranges of 10.0±1.6 to 15.5±1.9 mm against bacterial isolates and 11.3±2.1 to 13.4±1.2 mm against P. digitatum. However, these extracts did not exhibit any visible inhibition zone against C. albicans. Additionally, the minimum inhibitory concentrations (MICs) of these extracts were also determined and was found to be in the range of <31.25 to 1000 µg/mL. From this study, we concluded that extracts of the stem bark of T. minuta showed a moderate to significant antimicrobial activities. T. minuta has been used in food and beverage industries as preservative, coloring and flavoring agents. T. minuta also finds therapeutic applications in the traditional medicine.

scholarly journals Anticandidal Potential of Stem Bark Extract from Schima superba and the Identification of Its Major Anticandidal Compound

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1587 ◽  
Author(s):  
Chun Wu ◽  
Hong-Tan Wu ◽  
Qing Wang ◽  
Guey-Horng Wang ◽  
Xue Yi ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Stem Bark ◽  
New Drugs ◽  
Diffusion Method ◽  
Bark Extract ◽  
Ergosterol Biosynthesis ◽  
Membrane Composition ◽  
Schima Superba ◽  
Stem Bark Extract ◽  
Promising Source

Plant-derived extracts are a promising source of new drugs. Schima superba is traditionally used in China for heat clearing, detoxification, and treatment of furuncles. In this study, the anticandidal properties and mechanism of action of S. superba (SSE) were explored using a stem bark extract. SSE possessed high polyphenol and saponin contents of 256.6 ± 5.1 and 357.8 ± 31.5 µg/mg, respectively. A clear inhibition zone was observed for C. albicans growth through the disc diffusion method and the 50% inhibition of C. albicans by SSE was 415.2 µg/mL. Transcriptomic analysis in C. albicans treated with different doses of SSE was conducted through RNA-seq. Average values of 6068 genes and 20,842,500 clean reads were identified from each sample. Among these samples, 1680 and 1956 genes were differentially expressed genes (DEGs) from the SSE treatments of 0.2 and 0.4 mg/mL, respectively. C. albicans growth was inhibited by the changes in gene expression associated with the cell wall and membrane composition including the regulation of chitin degradation and ergosterol biosynthesis. This result could be reflected in the irregularly wrinkled morphology of the ruptured cell as revealed through SEM analysis. ESI-MS and NMR analyses revealed that the major compound purified from SSE was sasanquasaponin III and the 50% inhibition of C. albicans was 93.1 µg/mL. In summary, the traditional Chinese medicine S. superba can be applied as an anticandidal agent in complementary and alternative medicine.

scholarly journals Aktivitas Antibakteri Ekstrak Metanol dari Kulit Batang Kelor (Moringa oleifera Lam.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Nurul Cholifah ◽  
Ahmad Ridhay ◽  
Pasjan Satrimafitrah ◽  
Ruslan ◽  
Hardi Ys
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Moringa Oleifera Lam

Antibacterial activity of Moringa oleifera Lam. stem bark has been tested against Staphylococcus aureus and Escherichia coli. The extraction of Moringa oleifera Lam. stem bark was used maceration method with methanol solvent and has obtained extract yield of 6.1%. The antibacterial activity test of Moringa oleifera stem bark extracts used a well diffusion method. The concentration of Moringa oleifera stem bark extract was varied to four concentrations of 1% 2% 3% 4% (w/v). The inhibition zone of methanol extract of Moringa oleifera stem bark against Staphylococcus aureus at extract concentrations of 1%, 2%, 3%, and 4% was 10.08 mm, 11.8 mm, 15.00 mm, and 17.02 mm, respectively. The methanol extract of Moringa oleifera stem bark at concentrations of 1%, 2%, 3%, and 4% could also inhibition of Escherichia coli growth with inhibition zone of 14.01 mm, 16.50 mm, 17.09 mm, and 17.10 mm, respectively. Keywords: Moringa Oleifera Lam., Antibacterial, Staphylococcus aureus, Escherichia coli

scholarly journals Antimicrobial activities of extracts from Urtica urens

Food Research ◽  
2020 ◽  
Vol 4 (5) ◽  
pp. 1487-1492
Author(s):  
M.K. Pillai ◽  
R.P. Matamane ◽  
S.B. Mekbib
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Root Extract ◽  
Fungal Isolates ◽  
Stem Bark Extract ◽  
Urtica Urens

The antibacterial and antifungal activities of various extracts from Urtica urens were evaluated against selected microorganisms using agar hole-plate diffusion method. A total of ten extracts namely U. urens hexane leaf extract (HLE), chloroform leaf extract (CLE), ethyl acetate leaf extract (ELE), methanolic leaf extract (MLE), hexane stem-bark extract (HSB), chloroform stem-bark extract (CSB), ethyl acetate stem-bark extract (ESB), methanolic stem-bark extract (MSB), chloroform root extract (CRT) and methanolic root extract (MRT) were prepared. These extracts were evaluated against two Gram-positive bacteria viz. Listeria monocytogenes and Staphylococcus aureus, four Gram-negative bacteria viz. Serratia marcescens, Pseudomonas aeruginosa, Escherichia coli (wild strain) and Escherichia coli (O157:H7) and two fungal isolates viz. Candida albicans and Penicillium digitatum. The inhibition zones of these extracts were found to be in the range of 9.0±0.0 to 40.5±0.7 mm against bacterial isolates and 16.0±4.2 to 17.5±4.9 mm against C. albicans. However, all these extracts exhibited no visible zone of inhibition against P. digitatum. Additionally, the minimum inhibitory concentrations (MICs) of these extracts were also determined and were found to be in the range of <31.25 to >1000 µg/mL against both bacterial and fungal isolates. From this study, we concluded that ELS, MLE, CRT and MRT showed promising antimicrobial activities.

scholarly journals Comparative Study of Ethanolic Wild African Nutmeg (Pycnanthus angolensis (Welw.) Stem Bark Extract Potentials and Selected Conventional Toothpaste against Hidden Resident Mouth Cavity Microfora

2020 ◽  
pp. 10-36
Author(s):  
Mouth cavity Microfora. ◽  
Teniola Temitayo Mary
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Frequency Distribution ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Percentage Frequency ◽  
Mouth Cavity ◽  
Stem Bark Extract ◽  
Streptococcus Ratti

The aim of the study is to evaluate and compare the antibacterial activity of ethanolic stem extract of (Wild African nutmeg) Pycnanthus angolensis (Welw.) and some commercially available toothpaste against bacteria isolated from the hidden resident mouth cavity microfora. Bacteria were isolated from swabs of apparently healthy individuals and were identified using Staining procedure biochemical tests and the use of Bergey’s manual of bacteria identification  The assay for antibacterial activity of Pycnanthus angolensis stem bark extract and the four toothpastes were determined using agar well diffusion method. The Gram positive bacteria isolated were Streptococcus sangus, Streptococcus ratti, Stomatococcus mucilaginous., Peptostreptococcus  sp., and Streptococcus mutans and the Gram negative bacteria were Veillonella atypical, Veillonella parvula, Veillonella dispar and Acidiaminococcus sp. Oral B toothpaste showed maximum efficacy of inhibition with inhibition zone diameter as wide as 20 mm at 100 mg/ml. Percentage frequency distribution of antibacterial activity of conventional toothpaste (Close-up) against hidden resident mouth cavity microfora depicts Acidaminococcus sp.13%, Veillonella parvula (10%), Veillonella dispar (12%), Peptostreptococcus  sp.(12%), Stomatococcus mucilaginous.(9%), Streptococcus ratti (13%), Veillonella atypical (11%), Streptococcus sangus (9%) and Streptococcus mutans (11%), Percentage frequency distribution of antibacterial activity of conventional toothpaste (Oral B toothpaste) against hidden resident mouth cavity microfora reveals Acidaminococcus sp.(11%,) Veillonella dispar (11%), Veillonella parvula (10%), Peptostreptococcus sp. (12%), Stomatococcus mucilaginous.(15%), Streptococcus ratti (11%), Veillonella atypical (8%), Streptococcus sangus (10%),  and Streptococcus mutans (12%), Percentage frequency distribution  of antibacterial activity of conventional toothpaste (MyMy toothpaste) against hidden resident mouth cavity microfora depicts Acidaminococcus sp.(12%), Veillonella dispar (9%), Veillonella parvula (8%), Peptostreptococcus sp.(10%), Stomatococcus mucilaginous.(16%), Streptococcus ratti (9%), Veillonella atypical (15%),Streptococcus sangus (9%) and Streptococcus mutans (12%), Percentage frequency distribution of antibacterial activity of conventional toothpaste (Olive toothpaste) against hidden resident mouth cavity microfora shows Acidaminococcus sp.(9%), Veillonella dispar (10%), Veillonella parvula (10%), Peptostreptococcus sp.(12%), Stomatococcus mucilaginous.(13%), Streptococcus ratti (10%) ,Veillonella atypical (17%), Streptococcus sangus (7%),  and Streptococcus mutans (12%). Pycnanthus Angolensis stem bark extract inhibited the growth of the oral bacterial isolates with of zones of inhibition diameter ranging from 6 mm to 17 mm at a concentration of 100mg/ml. Secondary metabolite (Phytochemical) screening shows the presence of flavonoids, tannins, saponins, alkaloids, reducing sugars, steroid, phenol, terpenoid, pyrrolozidine alkaloid, glycoside and cardiac glycoside with glycoside and terpenoid most present. However, anthraquinones and volatile oil were absent. With menial antibacterial activity, P. angolensis can be use in the formulation of herbal toothpaste. It should be advocated that Pycnanthus angolensis should be added to our convention toothpaste to improve the functional ingredient of the toothpaste and Plant-based traditional knowledge has become a recognized tool in search for new sources of drugs. It is clear that the use of these herbal plants can offer a platform for further research.

Bioactive Compounds and in vitro Antimicrobial Activities of Ethanol Stem Bark Extract of Trilepisium madagascariense DC.

2018 ◽  
Vol 14 (7) ◽  
pp. 901-912 ◽  
Author(s):  
Olufunmiso Olusola Olajuyigbe ◽  
Tolulope Esther Onibudo ◽  
Roger Murugas Coopoosamy ◽  
Anofi Omotayo Tom Ashafa ◽  
Anthony Jide Afolayan
Keyword(s):  
Bioactive Compounds ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Stem Bark Extract

scholarly journals Evaluation of Antimicrobial Activities of Garcinia latissima Miq. Stem Bark Extract

2017 ◽  
Vol 9 (1s) ◽  
pp. s56-s59 ◽  
Author(s):  
Neneng Siti Silfi Ambarwati ◽  
Berna Elya ◽  
Amarila Malik ◽  
Muhamad Hanafi
Keyword(s):  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Stem Bark Extract

scholarly journals BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES USING MANILKARA HEXANDRA (ROXB.) DUBARD STEM BARK EXTRACT AND IT’S PHYSICAL, CHEMICAL CHARACTERIZATION AND PHARMACEUTICAL EVALUATION

2019 ◽  
pp. 79-88
Author(s):  
A ANTONY LAWRENCE ◽  
J THOMAS JOSEPH PRAKASH
Keyword(s):  
Silver Nanoparticles ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Uv Visible

Objective: The present study was to synthesize nanoparticles using Manilkara hexandra stem bark extract its characterization and evaluating it by an antimicrobial and antioxidant assay. Methods: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) was done by mixing silver nitrate (1 mmol) and aqueous stem bark extract and it was analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, Field Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive Spectroscopy (EDAX), Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC). The antibacterial assay was done by a well diffusion method and also examined for antifungal assay was done by disk diffusion method and antioxidant potential Diphenyl-1-picryl hydrazyl (DPPH method) Results: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) is characterized by various techniques such as UV-visible absorption spectrum ranges from 430 nm to 440 nm indicate silver nanoparticles. The Fourier Transform Infrared Spectroscopy consists of biomolecules acts as capping agent to form silver nanoparticles. Field Emission Scanning Electron Microscopy shows particle size ranges from 15 nm to 50 nm. Energy Dispersive Spectroscopy shows the presence of Silver. X-ray Diffraction corresponds to face-centered lattice planes (111), (200), (220) and (311). Dynamic Light Scattering show the range of 68 nm and Zeta potential show the negative value of-17 nm which has high stability. Silver nanoparticles is also examined by Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC) this project the thermal stability of the nanoparticles. The aqueous stem bark is also examined by UV-visible absorption spectrum, Fourier Transform Infrared Spectroscopy (FTIR), and Gas Chromatography-Mass Spectrometry (GCMS). In GCMS 20 compounds were identified. Silver nanoparticles show high zone of inhibition in antimicrobial assays and act as a good antioxidant agent. Conclusion: It is eco-friendly, non-toxic, and it’s easy to synthesis and it shows good result in an antimicrobial and antioxidant assay can be applied in a pharmaceutical application.

scholarly journals ISOLATION OF SECONDARY METABOLITE COMPOUNDS AND ANTIBACTERIAL ACTIVITIES TESTS FROM HEXANE EXTRACT OF STEM BARK MELOCHIA UMBELLATA (HOUTT) STAPF VAR. DEGRABRATA K

2018 ◽  
Vol 11 (8) ◽  
pp. 457 ◽  
Author(s):  
Usman Usman ◽  
Muh Amir M ◽  
Nunuk Hariani Soekamto ◽  
Ahyar Ahmad ◽  
Maulidiyah Maulidiyah
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Bark Extract ◽  
Separation And Purification ◽  
Inhibitory Zone

Objectives: This research aims to determine the content of secondary metabolite compounds and antibacterial activity of stem bark extract Melochia umbellata (Houtt) Stapf var. degrabrata.Methods: M. umbellata stem bark was extracted by maceration using methanol solvent. Separation and purification were done by partitioning, fractionation with chromatography, and recrystallization. Antibacterial activity test of hexane extract and third isolate from the bark of M. umbellata was done by agar diffusion method against bacterium Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.Results: Phytochemical test showed that the hexane extracts of bark M. umbellata containing alkaloids and triterpenoids. Isolate D is a triterpenoid group compound, while FKa and FKb are steroid group compound. The hexane extract had the highest antibacterial activity against B. subtilis bacteria with inhibitory zone diameter 12.0 mm. Isolate D has a weak inhibitory effect on all test bacteria. The highest diameters inhibition zone of isolated FKa compound against B. subtilis and S. aureus bacteria was 18.0 mm and 13.0 mm, respectively, whereas, the highest diameter inhibition of zone FKb compound against B. subtilis bacteria with inhibitory zone was 12.0 mm.Conclusion: The FKa compound from the bark of M. umbellata has the potential to be antibacterial because the compound is able to inhibit bacterial growth with ˃14 mm obstacle zone, especially against B. subtilis bacteria.

scholarly journals Phytochemical Screening and Antimicrobial Activities of Lannea acida (a. rich) Stem Bark Extract

2020 ◽  
pp. 21-26
Author(s):  
O. A. Olatunji ◽  
C. I. Ihediuche ◽  
O. W. Bolaji ◽  
A. O. Akala ◽  
S. E. Edet ◽  
...  
Keyword(s):  
Cardiac Glycosides ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Klebsiella Pneumonia ◽  
Continuous Extraction ◽  
Antimicrobial Drugs ◽  
Stem Bark Extract ◽  
Plant Stem ◽  
Micro Organisms

The persistent resistance of micro-organisms necessitates the need to intensify studies on the use of possible strategies for proper control of pathogens using extracts from natural products. The stem bark of Lannea acida was extracted by two methods of extraction: Cold and Soxhlet (continuous) extraction. The extract of the stem bark was screened to confirm the presence of alkaloids, flavonoids, saponins, tannins and cardiac glycosides in all the samples of ethanol and hexane/acetone/methanol mixture extracts. The phytochemicals examined varied among the stem bark extracts of other solvents (hexane, chloroform, acetone, and methanol). The result of the antimicrobial test shows that the stem bark extracts exhibited efficacy against some microbes (Bacillus cereus, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa Staphylococcus aureus and Streptococcus pyogenes) that were employed for the study by exhibiting clear inhibition/clearance zones against these microbes which ranges from 06 mm -21 mm. The plant stem bark could therefore be seen as a potential natural source for useful antimicrobial drugs.

scholarly journals Uji efek antibakteri ekstrak batang akar kuning (Arcangelisia flava Merr.) terhadap bakteri Staphylococcus aureus dan Escherichia coli

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Angga Dehes Kaharap ◽  
Christy Mambo ◽  
Edward Nangoy
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Bark Extract ◽  
Root Extract ◽  
Root Bark ◽  
Modified Technique

Abstract: Indonesia has a high diversity of flora and fauna in the world. Many types of plants can be used as a medicinal plant; one of them is yellow root (Arcengelisia flava Merr.) which has been known empirically by the Dayak community in Central Kalimantan as natural herbs to treat jaundice. This study aimed to determine the antibacterial effect of the yellow root bark extract against Staphylococcus aureus and Escherichia coli. This study was conducted in the Laboratory of Pharmacology and the Laboratory of Microbiology Faculty of Medicine University of Sam Ratulangi. This was an experimental laboratory study using the Kirby-Bauer modified technique. Yellow root bark extract was obtained from maceration process using ethanol 96%. The antibacterial effect was tested by using the well diffusion method. The results showed that distilled water, the negative control, did not generate inhibition zone around the wells. Ciprofloxacin, the positive control, had the greatest diameter of inhibitory zone. The average diameter of inhibition zone generated by ciprofloxacin was 39.23 mm against Staphylococcus aureus and 40.95 mm against Escherichia coli. Meanwhile, yellow root extract generated an average diameter inhibition zone of 12.27 mm against Staphylococcus aureus and 14.44 mm against Escherichia coli. Conclusion: Yellow root bark extract potentially had antibacterial effect against Staphylococcus aureus and Escherichia coli. Keywords: antibacterial, yellow root bark, Staphylococcus aureus, Escherichia coli. Abstrak: Indonesia memiliki keberagaman flora dan fauna yang tinggi di dunia. Banyak jenis tanaman yang dapat dijadikan sebagai tanaman obat, salah satunya akar kuning (Arcangelisia flava Merr.) yang telah lama dikenal secara empiris oleh masyarakat dayak di Kalimantan Tengah sebagai tanaman herbal alami untuk mengobati penyakit kuning. Penelitian ini bertujuan untuk mengetahui efek antibakteri dari ekstrak batang akar kuning terhadap bakteri Staphylococcus aureus dan Escherichia coli dan dilakukan di Laboratorium Farmakologi, dan Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Sam Ratulangi. Jenis penelitian ini eksperimental laboratorium dengan metode modifikasi Kirby-Bauer. Uji efek antibakteri menggunakan metode sumuran. Esktrak batang akar kuning diperoleh dari proses maserasi dengan etanol 96%. Hasil penelitian memperlihatkan akuades sebagai kontrol negatif tidak menimbulkan zona hambat di sekitar sumur. Siprofloksasin sebagai kontrol positif memiliki diameter zona hambat yang paling besar. Rerata diameter zona hambat yang dihasilkan oleh siprofloksasin 39,23 mm terhadap Staphylococcus aureus dan 40,95 mm terhadap Escherichia coli. Ekstrak akar kuning menghasilkan rerata diameter zona hambat sebesar 12,27 mm terhadap Staphylococcus aureus dan 14,44 mm terhadap Escherichia coli. Simpulan: Ekstrak batang akar kuning berpotensi memiliki efek antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci: antibakteri, ekstrak akar kuning, staphylococcus aureus, escherichia coli

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