Antibacterial effect of leaf extracts of Bauhimia reticulate, Azadirachta indica and Cassia saemia on bacterial isolates from bovine mastitis

Author(s):  
FE Tata ◽  
AA Chukwuedo ◽  
MI Agba ◽  
AO Olabode
Author(s):  
D. A. Awomukwu ◽  
C. K. Anumudu ◽  
A. J. Ogbolosingha

The research was undertaken to investigate the comparative phytochemical and in-vitro antibacterial activity of the single and combined strengths of the leaf extracts of Ocimum gratissimum Linn and Gongronema latifolium Benth. on some enteric bacterial isolates. The sensitivity test and minimum inhibitory concentration (MIC) were carried out using a modified agar-well diffusion method.  The enteric bacterial isolates tested included Escherichia coli, Shigella dysenteriae, Salmonella Typhi and Enterobacter aerogenes.  Standard methods were applied to obtain the ethanol and aqueous leaf extracts. The ethanol extracts of O. gratissimum and G. latifolium produced highly significant inhibitory activity against all the enteric bacterial isolates tested.  Comparatively, the ethanol plant extracts were more potent than the commercially available drug, Ciprofloxacin and the aqueous plant extracts. The isolates were sensitive at a minimum inhibitory concentration of 1.25 mg/ml for the ethanol extract but varied from 2.5 mg/ml - 5.0mg/ml in the aqueous extract.  It was also observed that the synergistic antibacterial effect of the medicinal plant extracts was greater than the singular antibacterial effect of the individual plant extracts in both the ethanol and aqueous extracts. The potency of the individual extracts and the combined effect may be due to the presence of flavonoids, alkaloids, phenols, tannins and saponins in the leaves of the plants.  This study partly validates the use of the plant extracts in the treatment of disease caused by the enteric bacterial isolates by multiple traditional medicine practitioners in Nigeria, however, strict adherence to dosage is recommended.  The leaf extract is a potential source of the new drug if the components are purified and enhanced for treating infections caused by these enteric pathogens.


2017 ◽  
Vol 5 (1) ◽  
pp. 12987-12987 ◽  
Author(s):  
Leila Maleki ◽  
Tahereh Sadeghian-Rizi ◽  
Mostafa Ghannadian ◽  
Mohammad Hossein Sanati ◽  
Shahin Shafizadegan ◽  
...  

2012 ◽  
Vol 1 (2) ◽  
pp. 229 ◽  
Author(s):  
P. K. Baidoo ◽  
J. I. Adam

<p>The effects of ethanolic extract of neem, <em>Azadirachta indica</em> (Meliaceae), seeds and petroleum ether extract of <em>Lantana camara</em> leaves (Verbenaceae) on the populations of three cabbage pests, <em>Plutella xylostella</em>, <em>Brevicoryne brassicae</em> and <em>Hellula undalis</em> were studied. The study was conducted between January and April 2008. Extracts of the two plants were sprayed on cabbage plants to control these pests. A standard synthetic chemical insecticide (Mektin) was used as reference product. The experiment was conducted in a randomized complete block design and each treatment was replicated four times. The effects of plant extracts on the population dynamics of the pests’ species, the level of infestation and yield were assessed. Significantly more of the pests infested the control plants than the treated plants (<em>P</em>&lt; 0.01). The mean weight of cabbage heads on the sprayed plots was significantly heavier than that of the control unsprayed plots. The use of <em>A. indica</em> seeds and <em>L. camara</em> leaf extracts increased yield by 37.05% and 25.80%, respectively. Spraying the cabbage plants with the plant extracts significantly reduced the numbers of pests compared with the control plants. The use of these plant extracts can be incorporated into an overall control programme of these pests.</p>


2016 ◽  
Vol 42 (1) ◽  
pp. 41-48
Author(s):  
Trisha Saha ◽  
Shamim Shamsi

Anthracnose and blight were recorded on Houttuynia cordata Thunb. during April 2013 to December 2013. The isolated fungi from the symptomatic plants were identified as Alterneria alternata (Fr.) Keissler and Colletotrichum gloeosporoides (Penz.) Sacc. Ethanol leaf extracts of five plants viz.,Azadirachta indica L., Citrus limon L., Datura metel L., Sennaalata L. and Tagetes erecta L.were evaluated against the pathogenic fungi A. alternata and C. gloeosporoides at 5%, 10% and 20% concentrations in vitro. A. indica recorded as good inhibitor against the test fungi followed by C. limon, S. alata, D. metel and T.erecta. In vivo treatment also showed that A.indica is the most effective in controlling diseases at 10% concentration. The plants treated with A. indica were fresh and healthy up to one month of observation.J. Asiat. Soc. Bangladesh, Sci. 42(1): 41-48, June 2016


2019 ◽  
Vol 9 (2) ◽  
pp. 66-71
Author(s):  
Arfan ◽  
Ratnawati ◽  
Hasmari Noer ◽  
Lisa Indriani ◽  
Asli ◽  
...  

The purposes of this research the examine the effectiveness various concentrations neem leaf ekstract on the intensity of attack Liriomyza spp on shallot Lembah Palu variety . Field research was carried out in Guntarano Village, Tanantovea Subdistrict, Donggala Regency, Central Sulawesi.  This research conducted from February to April 2016 .The experiment was done using Randomized Complete Block  Design with three replications, consists of six  treatments, i.e. P0 : control (without aplication neem leaf ekstract); P1: neem leaf ekstract 10 gr /litre;  P2 : neem leaf ekstract 20 gr / litre; P3 : neem leaf ekstract 30 gr / litre; P4 : neem leaf ekstract 40 gr / litre; P5 : neem leaf ekstract 50 gr / litre. To find out the effect of threatment, the analysis of variance, and the real influence is continued with the standard  ά = 0.05 BNT test.  The results showed that use of seed and leaf extracts of neem plant can control Liriomyza spp attack, only 3,04 percent of attack rate and shallot Lembah Palu variety productivity is 3,02/area.


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