Studies on Antioxidant Potential of Ethanol and Chloroform Leaf Extracts of Azadirachta Indica (Neem)

2018 ◽  
Vol 8 (4) ◽  
Keyword(s):  
Azadirachta Indica ◽  
Antioxidant Potential ◽  
Leaf Extracts

scholarly journals The Effects of Extracts of Lantana camara (L.) and Azadirachta indica (A. Juss) on the Population Dynamics of Plutella xylostella, Brevicoryne brassicae and Hellula undalis on Cabbage

2012 ◽  
Vol 1 (2) ◽  
pp. 229 ◽  
Author(s):  
P. K. Baidoo ◽  
J. I. Adam
Keyword(s):  
Population Dynamics ◽  
Plant Extracts ◽  
Azadirachta Indica ◽  
Plutella Xylostella ◽  
Block Design ◽  
Petroleum Ether Extract ◽  
Brevicoryne Brassicae ◽  
Lantana Camara ◽  
Leaf Extracts ◽  
Hellula Undalis

<p>The effects of ethanolic extract of neem, <em>Azadirachta indica</em> (Meliaceae), seeds and petroleum ether extract of <em>Lantana camara</em> leaves (Verbenaceae) on the populations of three cabbage pests, <em>Plutella xylostella</em>, <em>Brevicoryne brassicae</em> and <em>Hellula undalis</em> were studied. The study was conducted between January and April 2008. Extracts of the two plants were sprayed on cabbage plants to control these pests. A standard synthetic chemical insecticide (Mektin) was used as reference product. The experiment was conducted in a randomized complete block design and each treatment was replicated four times. The effects of plant extracts on the population dynamics of the pests’ species, the level of infestation and yield were assessed. Significantly more of the pests infested the control plants than the treated plants (<em>P</em>&lt; 0.01). The mean weight of cabbage heads on the sprayed plots was significantly heavier than that of the control unsprayed plots. The use of <em>A. indica</em> seeds and <em>L. camara</em> leaf extracts increased yield by 37.05% and 25.80%, respectively. Spraying the cabbage plants with the plant extracts significantly reduced the numbers of pests compared with the control plants. The use of these plant extracts can be incorporated into an overall control programme of these pests.</p>

scholarly journals Analysis of bioactive pigments in coloured callus of Azadirachta indica for possible use as functional natural colourants

2019 ◽  
Vol 48 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Sharmilla Ashokhan ◽  
Sujatha Ramasamy ◽  
Saiful Anuar Karsani ◽  
Rashidi Othman ◽  
Jamilah Syafawati Yaacob
Keyword(s):  
Tissue Culture ◽  
Azadirachta Indica ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Content Type ◽  
Bioactive Properties ◽  
Green Callus

Purpose The purpose of this study is to evaluate the content of bioactive pigments in coloured callus of Azadirachta indica and to understand the correlation between the callus colours with their bioactive constituents, antioxidant properties and cytotoxicity. These assessments will yield valuable insight into the use of in vitro-derived pigments for possible use as functional natural colourants. Design/methodology/approach In this study, the authors have successfully developed a protocol to produce leaf-derived callus of various colours with enhanced content of bioactive pigments in A. indica through plant tissue culture. Comparative analysis of the pigments content (chlorophyll, carotenoid, phenolics and anthocyanins) in the coloured callus was conducted, followed by evaluation of its bioactive properties. The antioxidant properties against 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, ferric reducing antioxidant power and cytotox activities of the coloured callus extracts were also reported. Findings Callus of various colours were successfully produced in A. indica through plant tissue culture, and their valuable pigment content and bioactivity were evaluated. The green callus contained the highest amount of anthocyanin, followed by brown and cream callus. The total anthocyanin contents in both the green and brown callus was more than two-fold higher than that in cream callus. Contrasting observation was obtained for total phenolic content (TPC), where the TPC of cream callus was significantly higher than that in brown callus. Nevertheless, the green callus also exhibited the highest TPC. Green callus also contained the highest amount of total chlorophyll and carotenoid, as well as exhibited the highest antioxidant potential, and was found to be the only extract with active cytotox activity against SKOV-3 cells. Correlation analysis revealed that the excellent bioactivity exhibited by the coloured extracts was strongly correlated with the bioactive pigments present in the callus. Research limitations/implications The major bioactive compounds identified in the methanolic extracts of A. indica coloured callus are anthocyanins, phenolics, chlorophylls and carotenoids. Future research work should include improvements in the extraction and identification methods, which may lead to detection and determination of other compounds that could attribute to its bioactivity, to complement the findings of the current study. Practical implications This analysis provides valuable information on the application of plant tissue culture as an alternative source for sustainable production of major pigments with medicinal benefits in A. indica for possible use as functional natural colourants. Originality/value A comparative study on bioactive pigment production in coloured callus from A. indica leaves and its antioxidant potential and cytotoxicity is original. To the best of the authors’ knowledge, this is the first report detailing a comparative evaluation on the production of coloured callus in A. indica and its relative biochemical composition and bioactive properties.

scholarly journals Effects of botanicals against anthracnose and blight diseases of Houttuynia cordata Thunb

2016 ◽  
Vol 42 (1) ◽  
pp. 41-48
Author(s):  
Trisha Saha ◽  
Shamim Shamsi
Keyword(s):  
Azadirachta Indica ◽  
Pathogenic Fungi ◽  
Tagetes Erecta ◽  
Citrus Limon ◽  
Leaf Extracts ◽  
Houttuynia Cordata ◽  
Datura Metel ◽  
Houttuynia Cordata Thunb

Anthracnose and blight were recorded on Houttuynia cordata Thunb. during April 2013 to December 2013. The isolated fungi from the symptomatic plants were identified as Alterneria alternata (Fr.) Keissler and Colletotrichum gloeosporoides (Penz.) Sacc. Ethanol leaf extracts of five plants viz.,Azadirachta indica L., Citrus limon L., Datura metel L., Sennaalata L. and Tagetes erecta L.were evaluated against the pathogenic fungi A. alternata and C. gloeosporoides at 5%, 10% and 20% concentrations in vitro. A. indica recorded as good inhibitor against the test fungi followed by C. limon, S. alata, D. metel and T.erecta. In vivo treatment also showed that A.indica is the most effective in controlling diseases at 10% concentration. The plants treated with A. indica were fresh and healthy up to one month of observation.J. Asiat. Soc. Bangladesh, Sci. 42(1): 41-48, June 2016

scholarly journals EFEKTIVITAS EKSTRAK DAUN NIMBA (Azadirachta indica A. Juss) DALAM MENGATASI SERANGAN LIRIOMYZA SPP PADA TANAMAN BAWANG MERAH LEMBAH PALU

2019 ◽  
Vol 9 (2) ◽  
pp. 66-71
Author(s):  
Arfan ◽  
Ratnawati ◽  
Hasmari Noer ◽  
Lisa Indriani ◽  
Asli ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Azadirachta Indica ◽  
Attack Rate ◽  
Block Design ◽  
Field Research ◽  
Leaf Extracts ◽  
The Real ◽  
Randomized Complete Block Design ◽  
Central Sulawesi

The purposes of this research the examine the effectiveness various concentrations neem leaf ekstract on the intensity of attack Liriomyza spp on shallot Lembah Palu variety . Field research was carried out in Guntarano Village, Tanantovea Subdistrict, Donggala Regency, Central Sulawesi.  This research conducted from February to April 2016 .The experiment was done using Randomized Complete Block  Design with three replications, consists of six  treatments, i.e. P0 : control (without aplication neem leaf ekstract); P1: neem leaf ekstract 10 gr /litre;  P2 : neem leaf ekstract 20 gr / litre; P3 : neem leaf ekstract 30 gr / litre; P4 : neem leaf ekstract 40 gr / litre; P5 : neem leaf ekstract 50 gr / litre. To find out the effect of threatment, the analysis of variance, and the real influence is continued with the standard  ά = 0.05 BNT test.  The results showed that use of seed and leaf extracts of neem plant can control Liriomyza spp attack, only 3,04 percent of attack rate and shallot Lembah Palu variety productivity is 3,02/area.

scholarly journals Bioactivity and Volatile Profiling of Azadirachta indica Leaves for the Management of Maize Weevil, Sitophilus zeamais (Motsch.) Infestations

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
A.G.W.U. Perera ◽  
M.M.S.C. Karunaratne ◽  
S.D.M. Chinthaka
Keyword(s):  
Azadirachta Indica ◽  
Solid Phase ◽  
Biological Activities ◽  
Sitophilus Zeamais ◽  
Gas Chromatography Mass Spectrometry ◽  
Solid Phase Micro Extraction ◽  
Leaf Extracts ◽  
Maize Weevil ◽  
Biological Potential ◽  
Wide Range

Neem (Azadirachta indica A. juss), is known to possess a wide range of pharmacological properties and is thus commercially exploitable. Apart from its medicinal potential, a considerable progress has been achieved regarding biological potential and chemical composition of the leaves which is an ever-increasing interest to the scientific community. During this study, biological phenomena and secondary metabolite composition of A. indica leaves were examined in the management of Sitophilus zeamais on stored maize. Insecticidal and repellent potential of A. indica leaf powders were evaluated in both contact and fumigant forms. Phytochemical screening of 11 phyto constituents was performed following the standard procedures for n-hexane, dichloromethane, ethyl acetate, methanol and aqueous leaf extracts. Volatile profile of A. indica leaves was characterized by employing headspace-solid-phase micro extraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS). Over 60% weevil repellency was recorded at doses above 23.33%, whereas 100% and 67% contact and fumigation mortalities were observed respectively, 9 days after treatment at the dose of 33.33% and the respected LD50 values were 1.56 g and 4.48 g. Thirty two volatile compounds were identified in three distinct chemical classes (Monoterpenoid, sesquiterpenoid and purine nucleosides). γ-Elemene (24.06%), 3,7 (11)-eudesmadiene (6.83%), caryophyllene (6.40%), and 10s,11s-himachala-3(12),4-diene (6.36%) were the major constituents of neem leaf volatiles, followed by other compounds present in less than 4% which might be responsible for varied biological activities observed. Thus the odour impact of the bioassayguided study clearly implies that A. indica leaves can be harnessed against S. zeamais infestations.Keywords: Azadirachta indica, Sitophilus zeamais, headspace-solid-phase micro-extraction, insecticidal activity, repellency

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