scholarly journals Comparative Assessment of Bacteriological Quality of Drinking Water using Membrane Filtration and Multiple Tube Fermentation Methods

2020 ◽  
Vol 24 (5) ◽  
pp. 853-856
Author(s):  
O.O. Adebisi ◽  
A.A. Adetomiwa ◽  
I.D. Gbala
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Water Sources ◽  
Comparative Assessment ◽  
Plate Count ◽  
Water Industry ◽  
Who Guidelines ◽  
Faecal Coliforms

Comparative assessment of 13 different drinking water sources (sachet, bottled, pipe borne and borehole) was carried using the conventional direct coliform plate count (CPC), multiple tube fermentation (MTF) and membrane filtration (MF) techniques. Physicochemical analyses indicated that the water samples meet WHO guidelines for pH, temperature and turbidity. However, the bacteriological analyses showed that none of the drinkingwater source is potable according to WHO guidelines. The confirmatory and completed tests revealed the presence of faecal coliforms in some of the samples. Findings of this study demonstrate that CPC is a poor method to assess water potability while MTF and MF techniques compared relatively well. There is need to intensify effort at the monitoring of activities in the rapidly expanding packaged water industry with a view to improving the standards. Keywords: drinking water, membrane filtration, multiple tube fermentation, coliform

scholarly journals Bacteriological and Physicochemical Quality of Drinking Water in Adis Kidame Town, Northwest Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Baye Sitotaw ◽  
Mulu Geremew
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Physicochemical Parameters ◽  
Coliform Bacteria ◽  
Residual Chlorine ◽  
Waterborne Diseases ◽  
Who Guidelines ◽  
Faecal Coliforms ◽  
Potential Health

Drinking water pollution and the resulting waterborne diseases have been among the major public health burden in low-income countries such as Ethiopia. A high prevalence of waterborne diseases, up to 65%, has been reported in Adis Kidame Town (Ethiopia). Moreover, there have been poor waste disposal practices in this town. Thus, this study aimed to assess bacteriological and physicochemical drinking water quality in Adis Kidame Town to get insight into any potential health risks due to waterborne diseases. A total of 90 water samples were analyzed for enumeration of coliform bacteria and basic physicochemical parameters. In this study, total and faecal coliform counts (CFU/100 ml) ranged from 0 to 23 and 0 to 18, respectively. In all cases, 89 and 77% of the water samples in terms of total and faecal coliforms, respectively, did not comply with WHO guidelines. The highest level of coliforms was found in drinking water samples from households’ containers and taps, where 100% and 90% of samples were tested positive for total and faecal coliforms, respectively. Among the physicochemical parameters recorded, only temperature and residual chlorine did not comply with WHO guidelines. Temperature values in all samples ranged from 20 to 28°C, while the values of residual chlorine were below the recommended range in the 56.7% of water samples from the reservoir and almost in all (96.7%) water samples from the taps and households’ containers. High coliform count in the water system demands proper chlorination, regular inspection of the distribution line, and good hygienic practices to improve the microbiological quality of drinking water in Adis Kidame Town.

scholarly journals Domestic drinking water management: Quality assessment in Oforikrom municipality, Ghana

2021 ◽  
Vol 104 (3) ◽  
pp. 003685042110359
Author(s):  
Eugene Appiah-Effah ◽  
Emmanuel Nketiah Ahenkorah ◽  
Godwin Armstrong Duku ◽  
Kwabena Biritwum Nyarko
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Water Quality Management ◽  
Water Sources ◽  
Quality Analysis ◽  
Water Quality Analysis ◽  
Microbial Water Quality ◽  
Drinking Water Sources

Drinking water in Ghana is estimated at 79%, but this only represents the proportion of the population with access to improved drinking water sources without regard to the quality of water consumed. This study investigated the quality of household drinking water sources in the Oforikrom municipality where potable water requirements are on the rise due to an ever-increasing population. Both quantitative and qualitative methods were employed in this study. One Hundred households were randomly selected and interviewed on the available options for drinking water and household water treatment and safe storage. A total of 52 points of collection (POC) and 97 points of use (POU) water samples from households were collected for physicochemical and microbial water quality analysis. Amongst the available drinking water options, sachet water (46%) was mostly consumed by households. Water quality analysis revealed that the physicochemical parameters of all sampled drinking water sources were within the Ghana Standards Authority (GSA) recommended values expected for pH (ranging from 4.50 to 7.50). For the drinking water sources, bottled (100%, n = 2) and sachet water (91%, n = 41) showed relatively good microbial water quality. Generally, POC water samples showed an improved microbial water quality in comparison to POU water samples. About 38% ( n = 8) of the households practicing water quality management, were still exposed to unsafe drinking water sources. Households should practice good water quality management at the domestic level to ensure access to safe drinking water. This may include the use of chlorine-based disinfectants to frequently disinfect boreholes, wells and storage facilities at homes.

scholarly journals Bacteriological Quality of Drinking Water Sources and Reservoirs Supplying Dharan Municipality of Nepal

2016 ◽  
Vol 2 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Narayan Dutt Pant ◽  
Nimesh Poudyal ◽  
Shyamal Kumar Bhattacharya
Keyword(s):  
Drinking Water ◽  
Membrane Filter ◽  
Water Sources ◽  
Plate Count ◽  
Indicator Organisms ◽  
Filter Technique ◽  
Coliform Count ◽  
Drinking Water Sources ◽  
Bacteriological Quality

BACKGROUND: Nepal is one of the least developed countries and in most of the areas the water supplied directly from natural water sources (like streams, rivers etc.) is used for drinking purpose, without any prior treatment. So every year there are many water related outbreaks mainly in rainy season. Very little data is available about the bacteriological quality of the water from drinking water sources, in the country. This report presents a scenario of the bacteriological quality of the water from drinking water sources and reservoirs, supplying Dharan municipality of Nepal.METHODS: Bacteriological analysis of the water samples was performed by membrane filter technique and spread plate technique. Total coliform count (TCC), fecal coliform count (FCC) and fecal streptococcal count (FSC) were performed by using membrane filter technique and total plate count (TPC) was performed by spread plate method.RESULTS: Most of the sources and reservoir tanks were found to be heavily contaminated with heterotrophic bacteria, total coliforms, fecal coliforms and fecal streptococci. Contamination with more than one types of indicator organisms was common.CONCLUSIONS: Most of the sources and reservoirs were found to be heavily contaminated with indicator organisms suggesting the alarming situation of water pollution in the area. Abrupt action is needed to be taken to improve the bacteriological quality of the water sources and reservoirs, supplying drinking water to Dharan municipality of Nepal. 

Assessment of microbiological quality of drinking water from household tanks in Bermuda

2008 ◽  
Vol 54 (6) ◽  
pp. 495-500 ◽  
Author(s):  
B. Lévesque ◽  
D. Pereg ◽  
E. Watkinson ◽  
J.S. Maguire ◽  
L. Bissonnette ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Microbiological Quality ◽  
General Information ◽  
Total Coliforms ◽  
E Coli ◽  
Electoral Register ◽  
Highly Correlated

Bermuda residents collect rainwater from rooftops to fulfil their freshwater needs. The objective of this study was to assess the microbiological quality of drinking water in household tanks throughout Bermuda. The tanks surveyed were selected randomly from the electoral register. Governmental officers visited the selected household (n = 102) to collect water samples and administer a short questionnaire about the tank characteristics, the residents’ habits in terms of water use, and general information on the water collecting system and its maintenance. At the same time, water samples were collected for analysis and total coliforms and Escherichia coli were determined by 2 methods (membrane filtration and culture on chromogenic media, Colilert kit). Results from the 2 methods were highly correlated and showed that approximately 90% of the samples analysed were contaminated with total coliforms in concentrations exceeding 10 CFU/100 mL, and approximately 66% of samples showed contamination with E. coli. Tank cleaning in the year prior to sampling seems to protect against water contamination. If rainwater collection from roofs is the most efficient mean for providing freshwater to Bermudians, it must not be considered a source of high quality drinking water because of the high levels of microbial contamination.

scholarly journals Quality and Safety of Rural Community Drinking Water Sources in Guto Gida District, Oromia, Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Motuma Tessema Abegaz ◽  
Mulissa Jida Midekssa
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Rural Community ◽  
Water Samples ◽  
Water Source ◽  
Total Coliform ◽  
Water Sources ◽  
Iron Manganese ◽  
Dug Well

The quality of drinking water has always been a major public health concern, especially in developing countries where access to improved water supply and sanitation is very low. This study aimed to assess the bacteriological and physicochemical quality of rural community drinking water sources in the Guto Gida district. A cross-sectional study was conducted in selected rural areas of the district from January to June 2016. Water samples were collected from four types of sources (protected dug well, open dug well, protected spring, and open spring) found in 8 locations of the study area. The membrane filtration technique was employed to determine the total coliform and faecal coliform load of the samples. The physicochemical characteristics such as total dissolved solid (TDS), pH, electrical conductivity (EC), turbidity, temperature, color, iron, manganese, lead, fluoride, zinc, sulphate, nitrate, and phosphate were analyzed following the American Public Health Association and WHO standard protocols. Our results revealed that 90.6% and 87.5% of water samples were positive for total coliform and faecal coliform, respectively. Thus, the majority of the studied water sources could be classified as polluted with respect to coliform load. Our results also have shown that most of the water sources showed marginally tolerable quality with respect to color, EC, TDS, turbidity, nitrate, sulphate, and phosphate. However, the protected sources had poor quality in zinc, lead, iron, manganese, and pH with values above the permissible levels. Thus, the drinking water source quality of the study areas requires appropriate interventions such as improving the existing water source infrastructure and access to sanitation services.

scholarly journals A Study on the Microbiological Status of Mineral Drinking Water

2017 ◽  
Vol 11 (1) ◽  
pp. 31-44 ◽  
Author(s):  
Faria Y. Aditi ◽  
Shafkat S. Rahman ◽  
Md. M. Hossain
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Mineral Water ◽  
Total Coliform ◽  
Plate Count ◽  
Dhaka City ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Susceptibility Tests ◽  
Susceptibility Tests

Introduction:Water-borne diseases constitute a major health burden in Bangladesh. The objective of this study was to assess the overall quality of mineral water samples that obtained from different shops of Dhaka city.Material and Methods:To achieve the above-mentioned objective, methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Moreover, isolated colony from mineral water samples were characterized by using biochemical and antimicrobial susceptibility tests.Results:Different water samples showed different HPC ranged from 1.0×10 to 8.00×102. Antimicrobial sensitivity test of some selected bacteria vizS. intermedius, S. aureus, S. felisandS. Saccharolyticuswere performed. It was observed thatStaphylococcusspp. isolates were susceptible to erythromycin, tetracycline, norfloxacin and ciprofloxacin. Furthermore, a fewStaphylococcusspp. isolates were intermediate resistant to penicillin and oxacillin. However, most of theStaphylococcusspp. isolates were resistant to cefixime.Conclusion:The results indicate that mineral water serves as a reservoir of various bacteria and that people in Dhaka city, who are the consumers of these water, might get diseases. This study emphasizes the need for elaborated microbiological examinations of mineral drinking water commonly used in Dhaka city.

scholarly journals Microbial assessment of bottled drinking water of Kathmandu valley

2013 ◽  
Vol 1 (2) ◽  
pp. 84-86 ◽  
Author(s):  
M Timilshina ◽  
I Dahal ◽  
B Thapa
Keyword(s):  
Drinking Water ◽  
Membrane Filtration ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Bottled Water ◽  
Kathmandu Valley ◽  
Who Guidelines ◽  
Cross Sectional

INTRODUCTION: Due to increased demand and consumption of bottled water in Kathmandu valley, there has been a growing concern about the microbiological quality of this product. The objective of this study was to assess the microbial contamination of heterotrophs and total coliform. MATERIALS AND METHODS: This cross sectional study was done in randomly selected 30 different commercial brand of bottled water available in commercial market of Kathmandu valley, Nepal. Total coliform and heterotrophic bacteria were enumerated by the membrane filtration and spread plate technique, respectively. RESULTS: Nineteen samples (63.3%) showed the heterotrophic bacterial count above the acceptable range (i.e. <50 cfu/ml) and 56.6% (n=17) samples showed the total coliforms count crossed the WHO guidelines (i.e. 0 cfu/ml). 83.3% (n=25) brands of bottled water were not safe for drinking as they exceed the heterotrophic and/or total coliform count above the WHO guidelines. CONCLUSIONS: The presence of heterotrophs and the coliform in the bottled water is a serious concern for public health. Concerned authority should prioritize this issue and continuously monitor the quality of mushrooming bottled water manufacturers in Kathmandu valley to provide safe drinking water to the population. DOI: http://dx.doi.org/10.3126/ijim.v1i2.7399 Int J Infect Microbiol 2012;1(1):84-86

Scope of potential bacterial agents of diarrhoea and microbial assessment of quality of river water sources in rural Venda communities in South Africa

2003 ◽  
Vol 47 (3) ◽  
pp. 59-64 ◽  
Author(s):  
C.L. Obi ◽  
N. Potgieter ◽  
P.O. Bessong ◽  
G. Matsaung
Keyword(s):  
South Africa ◽  
Heterotrophic Bacteria ◽  
Well Being ◽  
Water Sources ◽  
Plate Count ◽  
Human Consumption ◽  
Faecal Coliforms ◽  
Domestic Water ◽  
Total Coliforms

The microbial quality of several, usually untreated, surface domestic water sources, used by rural communities in the Venda Region of South Africa, was assessed to gauge their fitness for human consumption and to highlight the possible impact of waterborne diseases. The water sources studied were six points on the Levubu River and the rivers Mutale, Ngwedi, Tshinane, Makonde, Mutshindudi and Mudaswali. Total and faecal coliform, heterotrophic bacteria, enterococci and coliphage counts were used as indicators/surrogates to estimate the degree of bacterial and viral contamination respectively by standard methods. The presence of potential bacterial agents of diarrhoea such as Salmonella, Shigella, Campylobacter, Plesiomonas, Aeromonas and Vibrio was also determined. Results showed that the ranges of counts with regard to all the water sources investigated were 2.9 × 102 - 6.3 × 104 CFU/100 mL for faecal coliforms, 6.0 × 102 - 3.7 × 104 CFU/100 mL for total coliforms, 1.8 × 102 - 1.3 × 106 CFU/mL for heterotrophic plate count, 1.0 × 101 - 3.7 × 104 CFU/100 mL for enterococci and 0-13 PFU/100 mL for coliphages. These values are far higher than the acceptable maximum limits prescribed for South Africa by the Dept of Water & Forestry and the Water Research Commission - 0 CFU/100 mL, 5 CFU/100 mL, 1.0 × 102 CFU/mL, 0 CFU/100 mL and 1 PFU/100 mL for faecal coliforms, total coliforms, heterotrophic bacteria, enterococci and coliphages respectively. Salmonella, Shigella, Vibrio cholerae, Campylobacter, Aeromonas and Plesiomonas were isolated from several of the water sources investigated. The use of these water sources for drinking and domestic purposes poses a serious threat to the health and well being of the users and calls for urgent government intervention.

Bacteriological quality of drinking water at point of use and hand hygiene of primary food preparers: implications for household food safety

2016 ◽  
Vol 6 (2) ◽  
pp. 224-230
Author(s):  
S. G. D. N. Lakshmi Reddi ◽  
R. Naveen Kumar ◽  
G. M. SubbaRao ◽  
M. Vishnu Vardhana Rao ◽  
R. V. Sudershan
Keyword(s):  
Drinking Water ◽  
Food Safety ◽  
Hand Hygiene ◽  
Low Income ◽  
Water Samples ◽  
Faecal Coliforms ◽  
E Coli ◽  
Point Of Use ◽  
Drinking Water Samples

Quality of water for consumption and food processing activities is universally accepted as an essential component to ensure food safety at household (HH) level. Along with safe water, hand hygiene is also an important factor for reducing diarrheal illnesses. This was a cross-sectional study conducted in rural and urban HHs to detect hygiene indicators in drinking water samples at point of use (PoU) (n = 150) and their association with the hand hygiene of primary food preparers (n = 150). Overall, 24.7% and 9.3% of drinking water samples (PoU), 48% and 20% of hand rinse samples were contaminated by faecal coliforms and E. coli, respectively. Both drinking water (PoU) and hand rinse samples collected from rural HHs showed higher contamination, followed by those from urban slums and low income HHs. Significant association (p &lt; 0.05) and probable risk with faecal coliforms (OR. 2.5; 95% CI: 1.1–5.4) and E. coli (OR. 14.5; 95% CI: 4.1–50.7) was found between hand rinses and drinking water samples that had bacteriological contamination. These results suggest that there was an extensive cross contamination at HH level. So, targeted education is essential on safe food/water handling practices in HHs to prevent food safety risks.

scholarly journals Bacteriological Quality of Drinking Water and Associated Factors at the Internally Displaced People Sites, Gedeo Zone, Southern Ethiopia: A Cross-sectional Study

2021 ◽  
Vol 15 ◽  
pp. 117863022110264
Author(s):  
Zemachu Ashuro ◽  
Mekonnen Birhanie Aregu ◽  
Girum Gebremeskel Kanno ◽  
Belay Negassa ◽  
Negasa Eshete Soboksa ◽  
...  
Keyword(s):  
Drinking Water ◽  
Cross Sectional Study ◽  
Water Sources ◽  
Faecal Coliforms ◽  
Cross Sectional ◽  
Internally Displaced ◽  
Faecal Contamination ◽  
Bacteriological Quality ◽  
Sources Of Pollution

Background: Providing safe and adequate Water, Sanitation and Hygiene (WASH) services in response to emergencies is a major problem. Globally, few studies have investigated the bacteriological quality of drinking water at the IDP sites. Therefore, the objective of this study was to evaluate the bacteriological quality of drinking water and associated factors at the IDP sites of Gedeo zone. Methods: A cross-sectional study was conducted on 213 water samples collected from November to December 2018. The membrane filter technique was used to retain bacteria and lauryl sulphate broth media was used to detect faecal coliforms. After incubation of 14 hours at 44°C, faecal coliforms with yellow colonies were counted and expressed in terms of CFU/100 ml of water. The data were entered into Epi data version 3.1 and exported to STATA version 14 for analysis. Binary logistic regression analysis with 95%CI and P ⩽ .05 was used to identify factors associated with an outcome variable. Results: Out of 213 water samples collected, 107 (50.2%) samples were tested positive for faecal coliforms. The presence of latrines in uphill (AOR: 6.7, 95%CI: 1.0-42.9), other sources of pollution (AOR: 5.0, 95%CI: 1.1-22.3), inadequate fencing (AOR: 7.1, 95%CI: 1.3-40.2) and lack of diversion ditch (AOR: 6.3, 95%CI: 1.0-37.6) were factors significantly associated with faecal contamination of springs. Dug wells that had a latrine within 10 m (AOR: 11.4, 95%CI: 1.8-72.1), other pollution sources within 10 m (AOR: 7.9, 95%CI: 1.9-32.4), inadequate fencing (AOR: 2.8, 95%CI: 1.0-7.9), drawing water using a bucket with rope (AOR: 7.3, 95%CI: 1.6-33.4) and unsanitary well cover (AOR: 3.4, 95%CI: 1.1-10.4) were factors significantly associated with faecal contamination of wells. Conclusions: The majority of the water sources in internally displaced people sites were tested positive for faecal coliforms. The presence of latrine in uphill, other sources of pollution, inadequate fencing, lack of diversion ditch, drawing water using a bucket with rope and unsanitary well cover were factors associated with the presence of faecal coliforms. Therefore, adequate fencing, proper diversion ditch construction and hygiene promotion should be done to protect water sources from faecal contamination. Furthermore, latrines and other point sources of contamination should be located at least 10 m away or at a lower elevation from water sources.

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