scholarly journals Seed Germination of Faidherbia albida (Delile) A. Chev as Influenced by Different Pretreatments

2021 ◽  
Vol 25 (7) ◽  
pp. 1305-1309
Author(s):  
O.A. Iroko ◽  
I.L. Sowunmi ◽  
J.M. Ajekiigbe ◽  
S.O. Rufiai ◽  
W.T. Wahab

Faidherbia albida is an agroforesrty tree that has the potential of promoting agroforestry establishment in Nigeria. The seeds are glossy due to the presence of wax in the seed coat which prevents easy penetration of water. Thus, this study assessed the effect of different pretreatments (biological, mechanical and chemical) on the germination of F. albida seeds. The treatments include; seeds scarified at the helium, soaked in cold water for 24 hours, soaked in hot water for 3 minutes, 5 minutes, 10 minutes, & 15 minutes and soaked in Conc. H2SO4 for 3 minutes, 5 minutes, 10 minutes, and 15 minutes. The result showed that all treatment had uniform germination percentage of 100% but seeds soaked in H2SO4 for 15 min and 10 min had the highest germination value of (65.25) and (65.00) respectively, followed by 15mins soaking in hot water (47.14) while the least germination value was recorded in seeds scarified mechanically (33.31). Analysis of variance revealed that there was no significant difference in the treatments. However, seeds treated with H2SO4 at 15 mins and 10 mins had the best performance in terms of germination value compared with other treatments. Therefore, for optimum and uniform germination, the seed of F. albida seeds should be soak in concentrated H2SO4 for 15 min.

2017 ◽  
Vol 8 ◽  
Author(s):  
Amruta Shivajirao Shinde ◽  
Niranjana Sunil Chavan

<p><em>Ixora coccinea</em> L. is ornamentally and medicinally important coastal plant also associated with mangroves of Sindhudurg and Ratnagiri district. In present study seeds of <em>I. coccinea</em> were treated with cold water, 50°C  hot water, germinator solution and also 100 ppm, 200 ppm, 500 ppm GA. Germination percentage were measured every day until no germination were observed. Data was analysed using analysis of variance (ANOVA) and significant mean difference in pair of treatments was analysed by using t-Test. Seeds of <em>I. coccinea </em>treated with 100 ppm GA showed highest germination percentage i.e. 90% followed by hot water treated seeds and it  showed 85%  germination.<em> </em>Seeds were treated with GA, Hot water, Germinator, soaking in cold water showed significant difference in between treatments ( p&lt;0.05). Overall results suggest that 100 ppm GA and hot water treatment used for enhance the germination of <em>I. coccinea</em> seeds.</p>


2014 ◽  
Vol 2 (3) ◽  
pp. 83 ◽  
Author(s):  
Anita Luksi Indria Sandi ◽  
Indriyanto . ◽  
Duryat .

Nedun tree (Pericopsis mooniana) is one of commercial trees.  The propagation of nedun tree generatively has an obstacle due to it’s dormant character and the variety of seeds weight and the both factors are suspected influence the germanitaion.  This research aimed to determine effect of seed weight for nedun seed germination and the best of seeds weight for nedun seed germination; the effect of soaking in hot water at beginning temperature 80˚C by different soaking period on germination, and the best effect of soaking in hot water at beginning temperature of 80˚C by different soaking period on germination.  This research was compiled in a randomized complete block design, with 5 treatments and 3 groups.  Seeds were catagorized in to three group of weight which were heavy, medium, and light.  The treatments consisted of without scarification (as a control), soaking in hot water at beginning temperature of 80ºC during 12 hours, 24 hours, 36 hours, and 48 hours.  Every experiment unit consisted of 100 seed.  The observed variables were consisted of germination percentage, mean daily germination, germination value, and the ability to germinate.  Data analysis methods were used variance homogenity, variance analysis and least of difference test at 5% significant level.  Based on the analysis of variance showed that seeds weight significantly affected the mean daily germination, but had no effect on germination percentage, germination value, and the ability to germinate.  Seeds weight had a mean daily germination 15.088 days to germinate.  The medium seeds were germinated faster than heavy seeds and light seeds.  Seed scarification gave effect on germination percentage and germination value significantly, but not significant on mean daily germination and ability to germinate. Seeds scarification with hot water at beginning temperature 80°C for 48 hours had germination percentage 28,000%, germination percentage was better than the scarification for 36 hours, 24 hours, 12 hours, and control.


Agrologia ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Marthen Marthen ◽  
Elizabet Kaya ◽  
Herman Rehatta

In developing productive forests, seeds play a crucial role. The seeds of forests tree have physical condition of hard seed coat, especially Leguminoceae family. Silvicultural techniques that can overcome seed coat dormancy are dyeing and submersion of seeds. The knowledge and experience in breaking seed dormancy of Sengon (Paracerianthes falcataria L.) is rare, therefore it is necessary to study how to overcome seed dormancy. The objective of this study was to determine the effect of dyeing and submersion treatments to overcome seed dormancy of Sengon seed to improve its viability. This research used Completely Randomized Design (CRD) with two factors: namely Seed (Factor A), dipped in hot water at 60 º C for (A0 = Control, A1 = 2 minutes, A2 = 4 minutes, A3 = 6 minutes), Factor B, seeds soaked in cold water: (B0 = control, B1 = 6 hours of soaking, B2 = 12 hours, B3 = 18 hours, B4 = 24 hours). The parameters observed were germination percentage, germination rate and vigor index. The results showed that among all parameters observed the interaction of factors A1B2, A2B2 and A3B2 were the best treatment for germination percentage which was equal to 100 percent, the best germination rate of 3.897 days and the best vigor index of 27.02


Author(s):  
J. J. Cheboi ◽  
G. Mungabe

Seed dormancy in wheat (Triticum aestivum L.) is a major problem attributing to yield loss. It is a complex evolutionary trait that temporarily prevents seed germination, thus allowing seedling growth at a favorable season. This experiment was conducted to determine the effect of different pre-treatments on germination. The pre-germination treatments included mechanical scarification, soaking seeds in hot water at 100°C for 5 minutes, cold water for 24 hours and untreated (control). Two hundred seeds were used for each treatment. Seeds treated with cold and hot water commenced germination after 4 days and achieved 84%, 78.5% respectively germination within 10 days which was significantly different (P < 0.05) from other treatments, especially the untreated seeds which had the lowest germination of 30%, and commenced first germination after 10 days. The results showed significant differences (P < 0.05) in germination percentage and germination time. Results obtained in this experiment indicate that the pre-germination treatment of Farasi wheat seeds by using cold and hot water treatments can enhance germination of the seeds by breaking dormancy.


2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I. Lee

Abstract Background Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, propagation with seed germination of V. planifolia is intricate and unstable because the seed coat is extremely hard with strong hydrophobic nature. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla. Results We found that soaking mature seeds in 4% sodium hypochlorite solution from 75 to 90 min significantly increased germination. For the culture of immature seeds, the seed collection at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. Seeds at 60 DAP and subsequent stages germinated poorly. As the seed approached maturity, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope at maturity. On toluidine blue O staining, the wall of outer seed coat stained greenish blue, indicating the presence of phenolic compounds. As well, on Nile red staining, a cuticular substance was detected in the surface wall of the embryo proper and the innermost wall of the inner seed coat. Conclusion We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The window for successful germination of culturing immature seed was short. The quick accumulation of lignin, phenolics and/or phytomelanins in the seed coat may seriously inhibit seed germination after 45 DAP. As seeds matured, the thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, which may play an important role in inducing dormancy. Further studies covering different maturity of green capsules are required to understand the optimal seed maturity and germination of seeds.


2013 ◽  
Vol 5 (3) ◽  
pp. 325-331 ◽  
Author(s):  
Reyhaneh AZIMI ◽  
Hassan FEIZI ◽  
Mohammad KHAJEH HOSSEINI

The goal of this study was to evaluate concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L-1 with bulk TiO2 for possible stimulatory effects on wheatgrass seed germination and early growth stage. After 14 days of seed incubation, germination percentage improved by 9% following exposure to 5 ppm nanosized TiO2 treatment comparing to control. Similar positive effects occurred in terms of germination value and mean daily germination. Application of bulk TiO2 particles in 80 ppm concentration greatly decreased the majority of studied traits. Therefore phytotoxicity effect observed on wheatgrass seedling by application of bulk TiO2 particles in 80 ppm concentration. Exposure of wheatgrass seeds to 5 ppm nanosized TiO2 and bulk and nanosized TiO2 at 60 ppm obtained the lowest mean germination time but higher concentrations did not improve mean germination time. In general, there was a positive response by wheatgrass seed to some concentrations of nanosized TiO2. Usage of nanoparicles in order to improve germination and establishment of range plant in adverse environments similar to rangeland could be possible.


2021 ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I Lee

Abstract Background: Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, sexual propagation with seed germination of V. planifolia is intricate and unstable because of the extremely hard seed coat. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla.Results: We found that soaking mature seeds in 4 % sodium hypochlorite solution from 75 to 90 min significantly increased germination and that immature seeds collected at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. After 60 DAP, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope. These features matches the significant decreases of immature seed germination percentage after 60 DAP. Conclusion: We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, and might play an important role in seed dormancy of V. plantifolia.


2020 ◽  
Vol 16 (2) ◽  
pp. 296
Author(s):  
Neneng Laila Romdyah ◽  
Melya Riniarti ◽  
Ceng Asmarahman ◽  
Slamet Budi Yuwono

Scarification was the destruction of the seed coat to  softening seed coat and  became permeable to water and gas. The purpose of this study was to obtain the best scarification techniques, types of growth regulatory substances and the combination of scarification treatments and addition of growth regulatory substances to accelerate the germination of Pericopsis mooniana seeds. The study was conducted using factorial randomized block design with three replications. The first factor was the scarification technique, with hot water, and broken the seed skin. The second factor  was the addition of growth regulatory substances (coconut water and, bamboo shoots) shoot water and artificial growth regulatory substances  Dates analyzed with variance and continued with the tukey test at 5% level. The results showed that the scarification technique by broken seed skin significant differences in the percentage of sprouts, germination, average days of germination, vigor index, number of leaves, and seedling height. The addition of  growth regulatory substances did not show a significant difference in all observed variables.


Author(s):  
Amit Kumar Tiwari ◽  
Prawal P.S. Verma ◽  
Dipender Kumar ◽  
Sonveer Singh ◽  
Rakesh Kumar ◽  
...  

Background: Sarpagandha is an indigenous medicinal herb of Indian continent. It has many medicinal properties. Due to increasing demand of Sarpagandha, its exploitation is increasing continuously but for lack of cultivation, this plant has been listed in endangered category. Commercially, it is propagated through seeds but the main barrier of seed propagation is its irregular and low germination. Hence, the current study was undertaken to study the effect of growing media and seed treatment methods on seed germination and seedling growth of Sarpagandha to eliminate the inhibitory effect of the hard stony endocarp.Methods: Seed germination and seedling growth experiments of Sarpagandha were carried out with following different seed treatments, T1 (Control), T2 (Water soaking for 24 hours), T3 (water soaking for 48 hours), T4 (Water soaking for 24 hours + cotton cloth wrapping for 24 hours), T5 (GA3 200 ppm), T6 (FYM treatment for 24 hours), T7 (FYM treatment for 48 hours), T8 (Hot water treatment) and T9 (Cow dung treatment for 24 hours). Experiment was laid out in CRD. The data recorded on the various parameters were analyzed at 5% level of significance by using ANOVA.Result: Results indicated highest germination percentage (51), lowest mortality percent (49), minimum days for initiation of germination (23), minimum days taken for germination completion (33), highest speed of germination (3.77), maximum vigour index (1441.44), highest root (11.27 cm) and highest shoot length (17 cm) in T5 (GA3 200 ppm) followed by T3 (water soaking for 48 hours). Indicating, T5 (GA3 200 ppm) to be best seed treatment for higher germination and subsequent seedling growth of Sarpagandha (Rauvolfia serpentine L.).


2021 ◽  
Vol 19 (2) ◽  
pp. 1-9
Author(s):  
Juventine Boaz Odoi ◽  
David Mugeni ◽  
Robert Kiiza ◽  
Betty Apolot ◽  
Samson Gwali

Seed germination and seedling growth performance of Maesopsis eminii and Terminalia catappa under different water soaking treatments were evaluated for 120 days under nursery conditions. A total of 1400 seeds were pre-treated with hot (95oC) and cold water (ambient temperature) by soaking for 12, 24 and 48 hours with a control of no soaking. The seeds were sown directly into polythene pots filled with uniform growth medium (top forest soil, sand and clay soilmixed in a ratio of 5:3:2) to avoid disturbance of the root system after germination. The seeds were sown in a randomized block design with seven treatments and three replicates. Data were analysed using ANOVA in GenStat v18. Results indicated that soaking enhanced seed germination. Soaking of seeds in cold water for 12 hours resulted into higher germination (90% for Terminalia catappa and 85% for Maesopsis eminii) than the control (48%). Soaking period and water temperature significantly influenced seedling vigour (F value = 0.962; p = 0.038). Soaking seeds in cold water for 24 hours enhanced Maesopsis eminii seedling growth by 8.0 cm Terminalia catappa seedlings by 7.4 cm. Seed dormancy, germination percentage and growth performance in hard coated seeds such as Maesopsis eminii and Terminalia catappa can be broken by soaking in cold water for 12-24 hours. Pre-germination treatments significantly influences the germination and seedling growth. Keywords: Maesopsis eminii, Terminalia catappa, seeds, pre-germination, soaking.


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