scholarly journals Preliminary Evaluation of the Acute and Sub-Acute Anti-Inflammatory Activities of Aqueous and Butanol Leaf Fractions of Olax subscorpioidea Oliv. (Olacaceae).

2021 ◽  
Vol 19 (2) ◽  
pp. 398-409
Author(s):  
Odoma Saidi ◽  
Zezi A. Umar ◽  
Danjuma N. Muhammed ◽  
Ahmed Abubakar ◽  
M. Garba Magaji ◽  
...  
Keyword(s):  
Acetylsalicylic Acid ◽  
Acute Inflammation ◽  
Preliminary Evaluation ◽  
Distilled Water ◽  
Paw Edema ◽  
Experimental Animals ◽  
Inflammatory Conditions ◽  
Traditional Remedy ◽  
Cotton Pellet ◽  
Anti Inflammatory

Olax subscorpioidea (Oliv.) leaf is widely used as a traditional remedy for pain, reduction of small tumors, edema, painful swellings, and other inflammatory conditions. Its anti-inflammatory potential in experimental animals has been documented. Furthermore, an anti-inflammatory assay guided fractionation showed that the aqueous and butanol leaf fractions were the most active. This study is aimed at investigating further, the acute and sub-acute anti-inflammatory potentials of the aqueous and butanol leaf fractions in mice and rats.Carrageenan induced paw edema in rats, xylene induced ear edema in mice, and cotton pellet induced granuloma in rats were the models employed for the studies. For each of the methods, four randomly-selected groups of animals (n=5/6) were orally administered with distilled water (1 ml/kg), aqueous or butanol fraction (1,000 mg/kg) and standard drugs (acetylsalicylic acid [ASA] 300 mg/kg or dexamethasone, 1 mg/kg).The aqueous and butanol fractions each showed significant (p < 0.01) inhibition of ear swelling, the fractions also significantly (p < 0.05, p < 0.01) decreased the paw edema, and significantly (p < 0.01) inhibited the granuloma formation.The result of the study suggests that aqueous and butanol leaf fractions of Olax subscorpioidea are effective against acute and sub-acute inflammation.

scholarly journals Antinociceptive, anti-inflammatory and antipyretic properties of Melastoma malabathricum leaves aqueous extract in experimental animals

2006 ◽  
Vol 84 (12) ◽  
pp. 1291-1299 ◽  
Author(s):  
Z.A. Zakaria ◽  
R.N.S. Raden Mohd. Nor ◽  
G. Hanan Kumar ◽  
Z.D.F. Abdul Ghani ◽  
M.R. Sulaiman ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Distilled Water ◽  
Paw Edema ◽  
Hot Plate ◽  
Independent Manner ◽  
Experimental Animals ◽  
Traditional Uses ◽  
Melastoma Malabathricum ◽  
Anti Inflammatory ◽  
Abdominal Constriction

The present study was carried out to establish the antinociceptive, anti-inflammatory, and antipyretic properties of the aqueous extract of Melastoma malabathricum leaves in experimental animals. The antinociceptive activity was measured using abdominal constriction, hot-plate, and formalin tests, whereas the anti-inflammatory and antipyretic activities were measured using carrageenan-induced paw edema and brewer’s yeast-induced pyrexia tests, respectively. The extract, which was obtained after soaking the air-dried leaves in distilled water for 72 h and then preparing in concentrations of 10%, 50%, and 100% (v/v), was administered subcutaneously 30 min prior to subjection to the above mentioned assays. At all concentrations tested, the extract was found to exhibit significant (P < 0.05) antinociceptive, anti-inflammatory, and antipyretic activities in a concentration-independent manner. Our findings that the aqueous extract of M. malabathricum possesses antinociceptive, anti-inflammatory, and antipyretic activities supports previous claims on its traditional uses to treat various ailments.

ACUTE AND SUBACUTE ANTI-INFLAMMATORY ACTIVITIES OF DICHLOROMETHANE EXTRACT OF CASSIA ALATA (LINN.) LEAVES IN WISTAR RATS

2017 ◽  
Vol 15 (1) ◽  
pp. 174
Author(s):  
F. L Da ◽  
A. B Keugni ◽  
G. R Belemtougri ◽  
T. L. A Fotio ◽  
T. Dimo
Keyword(s):  
Traditional Medicine ◽  
Wistar Rats ◽  
Acute Inflammation ◽  
Control Group ◽  
Paw Edema ◽  
Strong Inhibition ◽  
Air Pouch ◽  
Cassia Alata ◽  
Cotton Pellet ◽  
Anti Inflammatory

Background: In Burkina Faso, leaves of Cassia alata are used in the treatment of several diseases, including inflammation. This study evaluated the anti-inflammatory effects of the dichloromethane extract of Cassia alata leaves (CF-AECal) on different models of inflammation in wistar rats in order to enhance its use in traditional medicine. Materials and Methods: Acute inflammation was induced among rats with 0.1 ml of carrageenan, serotonin, histamine and dextrane 1%. The effects of the CF-AECal 50 mg/kg and 100 mg/kg were compared to the effects of diclofenal 5 mg/kg, prednisone 5 mg/kg, promethazine 1 mg/kg, cyproheptadin 2 mg/kg. Cotton pellet and air pouch - induced granuloma permitted to study CF-AECal effects on the subacute inflammation. The ways of administrations were intra peritoneal for the substances of inductions and oral for the molecules of references and CF-AECal. Result: Carrageenan-induced paw edema has been inhibited to 52.39% by CF-AECal 50 mg/kg and 50.17% by CF-AECal 100 mg/kg. Wet granulomas formation has been inhibited by CF-AECal 50 and 100 mg/kg to 20.94 and 57.82%. The dry granulomas were inhibited at 24.32 and 59.04% by CF-AECal 50 and 100 mg/kg. Air pouch fluid was significantly reduced by CF-AECal in comparison to the control group. There was a massive recruitment of leukocytes in the pocket granuloma of the control group. CF-AECal caused strong inhibition of this infiltration of leukocytes (p< 0.001). Conclusion: Dichloromethane extract of Cassia alata showed anti-inflammatory effects.

Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

2019 ◽  
pp. 1-9
Author(s):  
Samira Salem ◽  
Essaid Leghouchi ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Time Course ◽  
Acute Inflammation ◽  
Lichen Species ◽  
Paw Edema ◽  
Sod Activity ◽  
Edema Formation ◽  
Lobaria Pulmonaria ◽  
Anti Inflammatory ◽  
Endogenous Antioxidant ◽  
Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

Effect of Nectaroscordum koelzi Methanolic Extract on Acute and Chronic Inflammation in Male Mice

2020 ◽  
Vol 20 ◽  
Author(s):  
Karam Hossein Hasanvand ◽  
Mojtaba Khaksarian ◽  
Maryam Alipour ◽  
Hormoz Mahmoudvand ◽  
Massumeh Naizi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Chronic Inflammation ◽  
Acute Inflammation ◽  
Methanol Extract ◽  
Significant Activity ◽  
Paw Edema ◽  
Ear Edema ◽  
Edema Volume ◽  
Cotton Pellet ◽  
Acute And Chronic Inflammation

Introduction: The present study deals with the effect of Nectaroscordum koelzi fruit extract on acute and chronic inflammation. Methods: Totally, 84 NMRI mice were used in this study. The extract effect on acute inflammation was analyzed by increasing vascular permeability via acetic acid and xylene induced ear edema among mice. The extract was evaluated in terms of effects on chronic inflammation by means of the cotton pellet test among mice. For the assessment of inflammation degree, the mice paw edema volume was measured by the plethysmometric test. Results: The findings showed that the extract was effective on acute inflammation induced by acetic acid in mice. In the xylene ear edema, N. koelzi extract indicated the significant activity in mice. In the cotton pellet method, the methanol extract produced a significant reduction in comparison with the control and dexamethasone. Mice paw edema volume decreased with the extract. Conclusion: In general, the data from the experiments indicated that the methanol extract of N. koelzi has an anti-inflammatory effect on acute and chronic inflammation. However, the exact contributing mechanisms have not been investigated for the pharmacological effects.

scholarly journals Adverse Effects of Subchronic Dose of Aspirin on Reproductive Profile of Male Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Archana Vyas ◽  
Heera Ram ◽  
Ashok Purohit ◽  
Rameshwar Jatwa
Keyword(s):  
Adverse Effects ◽  
Acetylsalicylic Acid ◽  
Serum Biochemistry ◽  
Lipid Profiles ◽  
Male Rats ◽  
Experimental Animals ◽  
Renal Functions ◽  
Sperm Density ◽  
Anti Inflammatory

Aspirin (acetylsalicylic acid) is widely used for cardiovascular prophylaxis and as anti-inflammatory pharmaceutical. An investigation was carried out to evaluate the influence of subchronic dose of aspirin on reproductive profile of male rats, if any. Experimental animals were divided into three groups: control and aspirin subchronic dose of 12.5 mg/kg for 30 days and 60 days, respectively, while alterations in sperm dynamics, testicular histopathological and planimetric investigations, body and organs weights, lipid profiles, and hematology were performed as per aimed objectives. Subchronic dose of aspirin reduced sperm density, count, and mobility in cauda epididymis and testis; histopathology and developing primary spermatogonial cells (primary spermatogonia, secondary spermatogonia, and mature spermatocyte) count were also significantly decreased in rats. Hematological investigations revealed hemopoietic abnormalities in 60-day-treated animals along with dysfunctions in hepatic and renal functions. The findings of the present study revealed that administration with subchronic dose of aspirin to male rats resulted in altered reproductive profiles and serum biochemistry.

scholarly journals Methanolic Extract of Leathery Murdah, Terminalia coriacea (Roxb.) Wight and Arn. Leaves Exhibits Anti-Inflammatory Activity in Acute and Chronic Models

2018 ◽  
Vol 27 (3) ◽  
pp. 267-271 ◽  
Author(s):  
Mohammed Safwan Ali Khan ◽  
Nishath Khatoon ◽  
Mohammad M. Al-Sanea ◽  
Mohamed Gamal Mahmoud ◽  
Hidayat Ur Rahman
Keyword(s):  
Methanolic Extract ◽  
Test Dose ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Inflammatory Models ◽  
Cotton Pellet ◽  
Wet Weight ◽  
Granulomatous Tissue ◽  
Anti Inflammatory

Objective: The aim of the present study was to evaluate anti-inflammatory activity of methanolic extract of Terminalia coriacea. Materials and Methods: A methanolic extract of T. coriacea leaves was studied in albino Wistar rats with carrageenan-induced paw edema, an acute model, and cotton pellet-induced granuloma, a chronic model, at 3 oral test doses (125, 250, and 500 mg/kg). Aspirin 100 mg/kg was used as a positive control. Paw volume and wet and dry weights of cotton pellets were determined. The data were analyzed by one-way ANOVA followed by Dunnett’s multiple comparison test. Results: The test extract at doses of 125 and 250 mg/kg decreased paw volume and wet and dry weights of cotton pellets. The highest test dose (500 mg/kg) displayed a response comparable to that of the standard drug (p < 0.01) on paw volume. The extract produced similar (p < 0.05) decrease in wet weight of the cotton pellet at 125 and 250 mg/kg, whereas the effect of 500 mg/kg of the extract was comparable to that of aspirin 100 mg/kg (p < 0.01). The extract of T. coriacea at 500 mg/kg induced the most significant (p < 0.01) effect on wet weight of granulomatous tissue. Conclusion: The methanolic extract of T. coriacea leaves successfully decreased paw edema as well as dry and wet weights of granulomatous tissue in both acute and chronic inflammatory models thus confirming the anti- edematogenic, antitransudative, and antiproliferative properties of T. coriacea.

scholarly journals Preliminary Analysis of the Anti-Inflammatory Activity of Essential Oils of Zingiber zerumbet

2010 ◽  
Vol 13 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Z. A. Zakaria ◽  
A. S. Mohamad ◽  
M. S. Ahmad ◽  
A. F. Mokhtar ◽  
D. A. Israf ◽  
...  
Keyword(s):  
Side Effects ◽  
Essential Oil ◽  
Preliminary Analysis ◽  
Formalin Test ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Zingiber Zerumbet ◽  
Cotton Pellet ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Nonsteroidal anti-inflammatory drugs (NSAIDs) have been widely used for the treatment of inflammation. However, despite their effectiveness, most NSAIDs cause various side effects that negatively affect the management of inflammation and, in part, pain. Thus, there is a need to search for new anti-inflammatory agents with few, or no, side effects. Natural products of plant, animal, or microorganism origin have been good sources of new bioactive compounds. The present study was carried out to evaluate the acute and chronic anti-inflammatory activities of the essential oil of the rhizomes of Zingiber zerumbet (Zingiberaceae) using the carrageenan-induced paw edema and cotton pellet-induced granuloma tests, respectively. The effect of the essential oil on inflammatory- and noninflammatory-mediated pain was also assessed using the formalin test. Essential oil of Z. zerumbet, at doses of 30, 100, and 300 mg/kg, was administered intraperitoneally to rats. The substance exhibited significant anti-inflammatory activity both in acute and chronic animal models. The essential oil also inhibited inflammatory- and noninflammatory-mediated pain when assessed using the formalin test. In conclusion, the essential oil of Z. zerumbet possessed anti-inflammatory activity, in addition to its antinociceptive activity, which may explain its traditional uses to treat inflammatory-related ailments.

Study of the anti-inflammatory and immunomodulatory activity of bovine placental extract

2019 ◽  
Vol 28 (03) ◽  
pp. 15-25
Author(s):  
Lkhagvasuren N ◽  
Bayar-Enkh B ◽  
Sainbileg G ◽  
Uyen E ◽  
Tsevgedorj Ch ◽  
...  
Keyword(s):  
Ammonium Salt ◽  
Mtt Assay ◽  
Stimulation Index ◽  
Distilled Water ◽  
Paw Edema ◽  
Rat Paw Edema ◽  
Dpph Method ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Placental Extract

Many scientists study structure and charagterization of placenta and experimenting on their biological activity, due to develop new drugs and biopreparation for human and animal health. The present study aimed to isolate bovine placental lactogen from placenta by semi purification method and determine antioxidant, anti-inflamatory activity and effect on immune responses was also investigation. Semi purified protein was separated  by gel-chromatographic method using 3 methods of tissue homogenizations of placental tissue using distilled water, phosphate buffer saline and Tris-HCl, by use of 4 methods of protein extraction and precipitation with distilled water, sulfate ammonium salt and organic solvents, such as ethanol and acetone.Antioxidant activity of semi purified  protein was measured by DPPH method, effect on immunity was investigated by using MTT assay kit, and anti-inflammatory activity was measured by using 1 % carrageenan-induced rat paw edema models. Measurements of total proteins which were homogenized in PBS, followed by acetone precipitation is 6217.5 µg/ml is the highest and homogenized in PBS, followed by sulfate ammonium salt precipitation is 1477.4 µg/ml is the lowest concentration. Bovine placental extract DPPH radical scavenging activity was 55.1±1.2% and rutin was 67.1±1.2% but results of immunity experiment demonstrated splenocyte division in placental extract added medium for stimulation index 1.45 or splenocyte culture increased by 1.5 times than as compared to those in medium not added placental extract, as well as placental extract added in the medium stimulation index increased 1.08 or splenocyte division by 0.8 times or almost the same than those wells with culture treated with mitogen only. Semi-purification placental protein was inhibition on 1% carrageenan induced rat paw edema and it means bovine placental extract effectively reduce acute inflammation. Үнээний эхэсийн хандны үрэвслийн эсрэг идэвх болон дархлаанд үзүүлэх нөлөөг судласан дүн Эхэс гэх эмийн түүхий эдийг дэлхийн олон эрдэмтэд бүтэц, физиологи, найрлага, биологийн идэвхт пептид ялган авч тэдгээрийн эмчилгээний идэвхийг судлаж улмаар хүн, мал, амьтны эрүүл мэнд, гоо сайханд хэрэглэх эм, биобэлдмэл гарган авах чиглэлээр олон талаас нь судалгаа, туршилтын ажлыг хийж байна. Энэхүү судалгааны зорилго нь үнээний эхэсээс хагас цэвэршүүлсэн уураг ялган антиоксидант идэвх, дархлаанд үзүүлэх нөлөөг in vitro орчинд судлах, үрэвслийн эсрэг идэвхийг туршилтын амьтны эмгэг загварт туршихад оршино. Эхэсийн ханд бэлтгэхдээ фосфатын буфер, ТрисHCl, нэрмэл усаар жигдрүүлж (гомогенизаци), цаашид тус бүр нэрмэл ус, ацетон, этанол, сульфат аммоны давс ашиглан 4 аргаар тундасжуулж хандлан гель хроматорафийн аргаар хагас цэвэршүүлсэн уураг ялган авав. Хагас цэвэршүүлсэн уургийн антиоксидант идэвхийг DPPH чөлөөт радикал саармагжуулах урвалаар, дархлаанд үзүүлэх нөлөөг MTT assay kit ашиглан, үрэвслийн эсрэг идэвхийг 1%-ийн Каррагининаар өдөөсөн хархны сарвуунд шүүдэст үрэсвлийн эмгэг загвар үүсгэн тус тус судлав. Хагас цэвэршүүлсэн уургийн концентрацийг тодорхойлоход фосфатын буферээр жигдрүүлж, ацетоноор тундасжуулсан хувилбарт уургийн хэмжээ хамгийн их 6217.5 мкг/мл, фосфатын буферээр жигдрүүлж, сульфат аммоны давсаар тундасжуулахад уургийн хэмжээ хамгийн бага 1477.4 мкг/мл байв. Үнээний эхэсийн хандны DPPH чөлөөт радикал саармагжуулах идэвх 55.1±1.2%, стандарт бодис Рутин 67.1±1.2% байсан бол дархлаанд үзүүлэх нөлөө үнээний эхэсийн ханд нэмсэн бүлгийг хяналтын бүлэгтэй харьцуулхад эс хуваагдлын индекс 1.45 буюу 1.5 дахин их, митоген нэмсэн бүлэгтэй харьцуулахад эс хуваагдлын индекс 1.08 буюу 0.8 дахин их буюу бараг адил хэмжээнд байлаа. 1%-ийн Каррагининаар үүсгэсэн хархын сарвууны шүүдэст үрэвсэлд үнээний эхэсээс ялгасан хагас цэвэршүүлсэн уураг нь үрэвслийн хаванг бууруулах нөлөөтэй ба үнээний эхэсийн ханд нь үрэвслийн эсрэг идэвхтэй байгаа нь тогтоогдов. Түлхүүр үг: Уураг, каррагинин, антиоксидантидэвх,шүүдэст үрэвсэл, хроматограф

scholarly journals Effects of Flavonoids from French Marigold (Florets ofTagetes patulaL.) on Acute Inflammation Model

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Ken Yasukawa ◽  
Yoshimasa Kasahara
Keyword(s):  
Arachidonic Acid ◽  
Oral Administration ◽  
Topical Application ◽  
Acute Inflammation ◽  
Topical Administration ◽  
Tagetes Patula ◽  
Paw Edema ◽  
Ear Edema ◽  
Inflammation Model ◽  
Anti Inflammatory

The major components patuletin and patulitrin were isolated from French marigold (florets ofTagetes patula). Patuletin and patulitrin were found to inhibit acute inflammation in mice. Oral administration of patuletin and patulitrin significantly suppressed hind-paw edema induced by carrageenin and histamine, while topical application of patuletin and patulitrin significantly inhibited ear edema induced by 12-O-tetradecanoylphorbol-13-acetate and arachidonic acid. Thus, oral and topical administration of patuletin and patulitrin inhibited acute inflammation in mice. These results suggest the anti-inflammatory efficacy of French marigold.

scholarly journals Evaluation of Anti-Inflammatory and Analgesic Activities of Methanolic Extract of Aitchisonia Rosea in Albino Mice

2020 ◽  
Vol 8 (1) ◽  
pp. 31-36
Author(s):  
Shahid Rasool ◽  
Ambreen Malik Uttra ◽  
Mehreen Malik Uttra ◽  
Faheem Ahmed ◽  
Khizar Abbas
Keyword(s):  
Acetic Acid ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Paw Edema ◽  
Egg Albumin ◽  
Inflammatory Conditions ◽  
Albino Mice ◽  
Anti Inflammatory

Objective:  Current analysis aims at in-vivo investigation of analgesic and anti-inflammatory activities of Aitchisonia rosea methanolic extract. Methods: Albino mice (20-30 g) were used in the study. Extract at 200, 400 and 800 mg/kg doses was tested against egg albumin and carrageenan induced mice paw edema along with formalin persuaded paw licking and acetic acid prompted writhing for analgesic activity. Results: Results depicted dose reliant and significant (p<0.001) inhibition of carrageenan as well as egg albumin brought paw edema, abdominal constrictions/ hind limb stretching tempted by acetic acid and formalin instigated paw licking. Capability of crude extract to halt inflammatory and analgesic response was akin to that of ibuprofen in all models. Conclusion: The current findings support the use of Aitchisonia rosea for inflammatory conditions, which might be attributed to its previously proven high alkaloid, flavonoids, phenol, tannins content and free radical scavenging activity.

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