scholarly journals Evaluation of Pathogenic Bacteria Associated with Fresh Produce obtained from Selected Markets in Abeokuta

2013 ◽  
Vol 4 (1) ◽  
pp. 75-81
Author(s):  
OR Afolabi ◽  
AR Oloyede ◽  
TA Ibrahim
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Fresh Produce ◽  
Enterobacter Aerogenes ◽  
Total Coliform ◽  
Shigella Dysenteriae ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Fresh Vegetables ◽  
Plate Counts

The bacterial quality of eight types of fresh produce obtained from selected markets in Abeokuta was determined. Two hundred forty (240) samples of fresh vegetables were examined for aerobic plate counts, coliform counts, and presence of Escherichia coli, toxigenic Staphylococcusaureus, Salmonella spp and Listeria spp. The aerobic plate counts ranged from 2.80 log10 cfu/g to 15.60 log10 cfu/g with the inner parts of cut- water melons having the highest value. Total coliform counts ranged from 0.0 to 11.80 log10 cfu/g. Pathogenic bacteria isolated were Escherichia coli,Staphylococcus aureus, Salmonella spp, Listeria spp, Shigella dysenteriae, Klebsiella pneumoniae, Enterobacter aerogenes, Bacillus spp, Pseudomonas aeruginosa and Streptococcus spp. These pathogens were mostly found at the outer leaves/ parts of the vegetables and render unsafe for human consumption. This study shows that the outer parts/ leaves of fresh produce are heavily contaminated with pathogenic bacteria and the fresh produce should be pre- treated thoroughly, so as to reduce the risk of food- borne outbreaks.Keywords · Pathogenic bacteria · Bacterial quality · Coliform counts

Comparing antibiotic resistance in commensal and pathogenic bacteria isolated from wild-caught South Carolina shrimps vs. farm-raised imported shrimps

2007 ◽  
Vol 53 (7) ◽  
pp. 919-924 ◽  
Author(s):  
Kavitha Boinapally ◽  
Xiuping Jiang
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
South Carolina ◽  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Vibrio Vulnificus ◽  
Resistant Bacteria ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Plate Counts

The objective of this study was to assess and differentiate wild-caught South Carolina (SC) shrimps from imported shrimps on the basis of microbiological analysis. Seven wild-caught SC shrimp and 13 farm-raised imported shrimp samples were analyzed. Total plate counts from wild-caught shrimp samples ranged from 4.3 to 7.0 log10 CFU/g, whereas counts from imported shrimp samples ranged from 3.2 to 5.7 log10 CFU/g. There was no difference (P > 0.05) between total bacterial counts of wild-caught SC shrimp and farm-raised imported shrimp. However, the percentages of bacteria with reduced susceptibility towards ceftriaxone and tetracycline were higher (P < 0.05) for farm-raised shrimp than for wild-caught samples. Salmonella spp. detected only in one farm-raised sample was resistant to ampicillin, ceftriaxone, gentamicin, streptomycin, and trimethoprim. Vibrio vulnificus was detected in both wild-caught and farm-raised shrimp samples; however, only the isolate from farm-raised shrimp was resistant to nalidixic acid and trimethoprim. Escherichia coli detected in one wild-caught sample was resistant to ampicillin. Both Listeria spp. and Salmonella spp. were absent with wild-caught SC samples. Therefore, the presence of more ceftriaxone- and tetracycline-resistant bacteria and the observed antimicrobial resistance phenotypes of isolates from the imported shrimp may reflect the possible use of antibiotics in raising shrimp in those countries.

Microbial Evaluation of Selected Fresh Produce Obtained at Retail Markets

2002 ◽  
Vol 65 (4) ◽  
pp. 677-682 ◽  
Author(s):  
RICHARD L. THUNBERG ◽  
TONY T. TRAN ◽  
REGINALD W. BENNETT ◽  
ROGER N. MATTHEWS ◽  
NEGASH BELAY
Keyword(s):  
Fresh Produce ◽  
Agar Plate ◽  
Total Coliform ◽  
Plate Count ◽  
Most Probable Number ◽  
Macconkey Agar ◽  
Salmonella Spp ◽  
Probable Number ◽  
Plate Counts ◽  
Campylobacter Spp

The microbial quality of five types of fresh produce obtained at the retail level was determined by standard quantitative techniques. These techniques included aerobic plate count (APC), total coliform counts, Escherichia coli counts, and yeast and mold counts. Three different methods were used to determine total coliform counts, which consisted of MacConkey agar plate counts, Colicomplete most probable number counts, and Petrifilm E. coli (EC) plate counts. The mean APCs for sprouts, lettuce, celery, cauliflower, and broccoli were 8.7, 8.6, 7.5, 7.4, and 6.3 log10 CFU/g, respectively. MacConkey agar counts indicated that 89 to 96% of the APCs consisted of gram-negative bacteria. Yeast and mold counts were in a range expected of fresh produce. Fresh produce was also analyzed for human pathogens. Samples were analyzed for Staphylococcus spp., Bacillus spp., Salmonella spp., Listeria spp., and Campylobacter spp. One isolate of Staphylococcus was found to be enterotoxigenic, and one species of Bacillus was also toxigenic. Neither Salmonella spp. nor Campylobacter spp. were detected in any of the produce samples. A variety of Listeria spp., including Listeria monocytogenes, were found in fresh produce.

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

scholarly journals Efficacy of a solar still in destroying virus and indicator bacteria in water for human consumption

2018 ◽  
Vol 13 (4) ◽  
pp. 1
Author(s):  
Felipe Tiago do Nascimento ◽  
Carlos Augusto do Nascimento ◽  
Fernando Rosado Spilki ◽  
Rodrigo Staggemeier ◽  
Cláudio Marcos Lauer Júnior
Keyword(s):  
Escherichia Coli ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Pilot Scale ◽  
Total Coliform ◽  
Water Disinfection ◽  
Most Probable Number ◽  
Human Consumption ◽  
Solar Still ◽  
Probable Number

Natural water distillation can destroy and/or inactivate microorganisms that are sensitive to heat and ultraviolet radiation (UV). This method is currently used to provide fresh water in ships and in the desalination of brackish water. For the development of this research, a pilot-scale solar still was built and installed in the southern region of Brazil, in order to assess its efficiency in water disinfection, which was based on the most probable number (MPN) of total coliforms and  Escherichia coli, in addition to the DNA copy number of human adenovirus type 5 (HAdV-5) in raw, undistilled samples and in treated distilled water. Results showed that the distillation process removed 100% of total coliform and Escherichia coli and 4.5 log (99.997%) of HAdV-5, which meets the microbiological standards for drinking water according to national Brazilian regulations, as well as USEPA and HEALTH CANADA requirements.

scholarly journals Preliminary assessment of small scale bacterial depuration of Crassostrea gigas and Anadara spp., Gulf of Nicoya, Costa Rica

2013 ◽  
Vol 5 ◽  
pp. 107
Author(s):  
Luis A. Vega Corrales ◽  
Carolina Marín Vindas ◽  
Oscar Pacheco Prieto ◽  
Gerardo Zúñiga Calero
Keyword(s):  
Escherichia Coli ◽  
Costa Rica ◽  
Crassostrea Gigas ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Small Scale ◽  
Bivalve Molluscs ◽  
Salmonella Spp ◽  
E Coli ◽  
Gulf Of Nicoya

Bivalve molluscs are sold without any sanitary control in Costa Rica, which represents a public health risk dueto the possible accumulation of pathogenic bacteria. Small scale bacterial depuration treatments were preliminarilyevaluated on Crassostrea gigas and Anadara spp. from the Gulf of Nicoya, Costa Rica, through the useof a recirculating system with UV irradiation. The levels of Escherichia coli in the water were determined usingthe MPN method. The MPN of E. coli and the presence of Salmonella spp., Vibrio parahaemolyticus and V. cholerae were determined in molluscs. Results confirm the effectiveness of the E. coli depuration system designin C. gigas and Anadara spp. The MPN of E. coli in the molluscs was reduced between 79% and 100% after 24h. None of the other types of bacteria were found in the samples analyzed. This is the first C. gigas depurationstudy in Costa Rica and the first report concerning UV depuration for bacterial indicators of fecal contaminationin Anadara spp. This research serves as a basis for the implementation and improvement of mollusc purification conditions in the country. It is recommended to monitor extraction and harvesting areas, as well as to implement the depuration of bivalve molluscs so that traditional producers can offer an innocuous product with added value.Los moluscos bivalvos son comercializados sin ningún control sanitario en Costa Rica; por tanto, representan un riesgo para la salud pública, debido a que pueden acumular bacterias patógenas. Se evaluaron preliminarmente tratamientos para la depuración bacteriana a pequeña escala de Crassostrea gigas y Anadara spp. en el Golfo de Nicoya, Costa Rica, utilizando un sistema recirculado e irradiado con luz UV. Los niveles de Escherichia coli en el agua se determinaron mediante el método del NMP. A los moluscos se les determinó el NMP de E. coli y la presencia de Salmonella spp., Vibrio parahaemolyticus y V. cholerae. Los resultados confirman la efectividad del diseño del sistema para la depuración de E. coli en C. gigas y Anadara spp. A las 24 h, el NMP de E. coli en los moluscos se redujo entre el 79% y el 100%. No se comprobó la presencia de las demás bacterias en las muestras analizadas. Este es el primer estudio sobre depuración de C. gigas en Costa Rica y el primer reporte de depuración con UV de indicadores bacterianos de contaminación fecal en Anadara spp. Este trabajo sirve de base para implementar y mejorar las condiciones de la depuración de moluscos en el país. Se recomienda monitorear las áreas de extracción y cultivo e implementar la depuración de los moluscos bivalvos para que los productores artesanales puedan ofrecer un producto inocuo y con valor agregado.

scholarly journals Public Health Safety in Relation to Microbiological Quality of Freshly Drawn Cow’s Milk in Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 231-236
Author(s):  
R Khaton ◽  
MA Hasnat ◽  
S Rahman ◽  
MM Rahman
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Viable Count ◽  
Microbial Quality ◽  
Total Viable Count ◽  
Coliform Count ◽  
Milk Samples

The aim of the present study was to determine the microbial quality of freshly drawn cow’s milk belonging to the arsenic affected and non-affected areas. In the present work 25 milk sample were collected from five different arsenic affected and non-affected areas. The analysis comprised enumeration of total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC) for the determination of sanitary quality. The highest total viable count, total coliform count and total staphylococcus count were log 5.894 ± 0.221, log 2.832± 0.129 and log 2.898 ± 0.162 respectively. Staphylococcus spp, Escherichia coli, Pseudomonas spp and Bacillus spp were isolated from the milk samples. Among them Escherichia coli were isolated from the maximum milk samples. It is found that the level of microbial quality in terms of TVC, TCC and TSC were high in arsenic affected areas than arsenic non-affected area. It also found that TVC and TCC were positively correlated with each other. This survey indicates that most of the raw milk samples were not satisfactory in course of public health standard as some pathogenic bacteria were detected from these samples and it is necessary to improve the hygienic practices required for the handling and processing of milk.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21297 Bangl. J. Vet. Med. (2014). 12 (2): 231-236 

Microbial Contamination Occurring on Lamb Carcasses Processed in the United States

2001 ◽  
Vol 64 (4) ◽  
pp. 503-508 ◽  
Author(s):  
E. A. DUFFY ◽  
K. E. BELK ◽  
J. N. SOFOS ◽  
S. B. LeVALLEY ◽  
M. L. KAIN ◽  
...  
Keyword(s):  
United States ◽  
Microbial Contamination ◽  
The United States ◽  
Total Coliform ◽  
Regulatory Requirements ◽  
Salmonella Spp ◽  
E Coli ◽  
Total Incidence ◽  
Tissue Surface ◽  
Plate Counts

Lamb carcasses (n = 5,042) were sampled from six major lamb packing facilities in the United States over 3 days during each of two visits (fall or winter, October through February; spring, March through June) in order to develop a microbiological baseline for the incidence (presence or absence) of Salmonella spp. and for populations of Escherichia coli after 24 h of chilling following slaughter. Samples also were analyzed for aerobic plate counts (APC) and total coliform counts (TCC). Additionally, incidence (presence or absence) of Campylobacter jejuni/coli on lamb carcasses (n = 2,226) was determined during the slaughtering process and in the cooler. All samples were obtained by sponge-sampling the muscle-adipose tissue surface of the flank, breast, and leg of lamb carcasses (100 cm2 per site; 300 cm2 total). Incidence of Salmonella spp. in samples collected from chilled carcasses was 1.5% for both seasons combined, with 1.9% and 1.2% of fall or winter and spring samples being positive, respectively. Mean (log CFU/cm2) APC, TCC, and E. coli counts (ECC) on chilled lamb carcasses across both seasons were 4.42, 1.18, and 0.70, respectively. APC were lower (P &lt; 0.05) in samples collected in the spring versus fall or winter, while TCC were higher in samples collected in the spring. There was no difference (P &gt; 0.05) between ECC from samples collected in the spring versus winter. Only 7 out of 2,226 total samples (0.3%) tested positive for C. jejuni/coli, across all sampling sites. These results should be useful to the lamb industry and regulatory authorities as new regulatory requirements for meat inspection become effective.

scholarly journals Detection of Enterobacteriaceae, antimicrobial susceptibility, and virulence genes of Escherichia coli in canaries (Serinus canaria) in northeastern Brazil

2019 ◽  
Vol 39 (3) ◽  
pp. 201-208
Author(s):  
Antonio Jackson F. Beleza ◽  
William C. Maciel ◽  
Arianne S. Carreira ◽  
Windleyanne G.A. Bezerra ◽  
Cecilia C. Carmo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Morphological Characteristics ◽  
Brain Heart Infusion ◽  
Enterobacter Aerogenes ◽  
Northeastern Brazil ◽  
Salmonella Spp ◽  
Pcr Screening ◽  
E Coli

ABSTRACT: This study aimed to verify the presence of members from the Enterobacteriaceae family and determine antimicrobial susceptibility profiles of the isolates in canaries bred in northeastern Brazil; in addition, the presence of diarrheagenic Escherichia coli (DEC) and avian pathogenic Escherichia coli (APEC) was also verified in these birds. Samples were collected during an exhibition organized by the Brazilian Ornithological Federation in July 2015 in Fortaleza, Brazil. A total of 88 fecal samples were collected and submitted to pre-enrichment step using buffered peptone water, followed by enrichment with the following broths: brain-heart infusion, Rappaport-Vassiliadis, and Selenite-Cystine. Subsequently, aliquots were streaked on MacConkey, brilliant green and salmonella-shigella agar plates. Colonies were selected according to morphological characteristics and submitted to biochemical identification and antimicrobial susceptibility tests with disk-diffusion technique. E. coli strains were evaluated for the presence of eight DEC genes and five APEC genes through conventional polymerase chain reaction (PCR) screening. The most frequent species observed were Pantoea agglomerans (25%), Serratia liquefaciens (12.5%), and Enterobacter aerogenes (9.1%). A single rough strain of Salmonella enterica subsp. enterica was identified in one sample (1.1%). High resistance rates to amoxicillin (78.7%) and ampicillin (75.4%) were identified. Polymyxin B (9.8%), gentamycin (6.6%), and enrofloxacin (6.6%) were the most efficient antibiotics. The total number of multidrug-resistant strains (isolates resistant to more than three antimicrobial classes) was 23 (37.7%). Four E. coli strains were tested for the virulence genes, and two were positive for APEC virulence genes: one strain was positive for iutA and the other for hlyF. In conclusion, canaries in northeastern Brazil participating in exhibitions may present Salmonella spp., Escherichia coli and other enterobacteria in the intestinal microbiota with antimicrobial resistance. These results indicate that, although the E. coli strains recovered from canaries in this study have some virulence genes, they still do not fulfill all the requirements to be considered APEC.

Microbial Populations on Hides of Grazing Steers in a Forage-Based Production System in Uruguay

2008 ◽  
Vol 71 (9) ◽  
pp. 1919-1921
Author(s):  
PABLO J. ROVIRA ◽  
JOSÉ I. VELAZCO
Keyword(s):  
Escherichia Coli ◽  
Environmental Conditions ◽  
Production System ◽  
Total Coliform ◽  
Red Meat ◽  
Microbial Populations ◽  
Plate Counts ◽  
Grazing Livestock ◽  
Microbiological Status ◽  
Hygienic Conditions

The objective of the study was to evaluate the microbiological status of hides of grazing steers in a typical forage-based system in Uruguay. The study was conducted on a single farm with samples taken on 3 days during the spring of 2007. Four anatomical hide sites (perineum area, flank, back, and shoulder) of 10 steers were individually swabbed each sampling day at the farm environment (n = 120). Each sample was analyzed by the Laboratorio Tecnológico del Uruguay for aerobic plate counts (APC), total coliform counts (TCC), and Escherichia coli counts (ECC). Mean log values for APC, TCC, and ECC on external animal hide surfaces, across all sampling sites, were 5.52, 1.89, and 1.70 log CFU/cm2, respectively. There were no significant differences among bacterial counts from the four hide surface locations. Mean log values for APC, TCC, and ECC were 1.49, 1.15, and 1.12 log CFU/cm2 lower, respectively, on sampling day 2 than on sampling day 3. Microbial populations on hides of grazing steers are highly variable and dependent on climatic and environmental conditions. To our knowledge this is the first study published evaluating the hygienic conditions of grazing livestock operations in Uruguay and their potential implications on the red meat chain.

Comparison of Forced-air Cooling with Static-air Cooling on the Microbiological Quality of Cooked Blue Crabs1

1992 ◽  
Vol 55 (2) ◽  
pp. 104-107
Author(s):  
DEOGHWAN OH ◽  
DOUGLAS L. MARSHALL ◽  
MICHAEL W. MOODY ◽  
J. DAVID BANKSTON
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Air Cooling ◽  
Plate Counts ◽  
Forced Air ◽  
Thermocouple Temperature ◽  
Microbiological Analyses

Microbiological analyses were made on samples of cooked blue crab taken immediately after debacking and either forced-air cooling or static-air cooling. Forced-air cooling had significantly lower (P&lt;0.05) total coliform and fecal coliform counts, 2.51 and 2.30 log10 MPN/100 g, compared with those of static-air cooling, 2.83 and 2.60 log10 MPN/100 g. All treatments had less than 2.30 log10 MPN/100 g Escherichia coli. Staphylococcus aureus counts in the forced-air cooled crabs were approximately 4-fold lower than counts in static-air cooled crabs. The aerobic plate counts and psychrotrophic plate counts were significantly lower (P&lt;0.01) by 1.04 and 0.81 log10 CFU/g, respectively, by forced-air cooling compared to static-air cooling. Thermocouple temperature readings were used to determine differences in cooling rates between forced-air and static-air cooling. After 1.5 h of cooling, the initial precooled crabmeat temperature of 34°C (93°F) was reduced by forced-air cooling and static-air cooling to 4°C (40°F) and 20°C (67°F), respectively. The rates of cooling using forced-air and static-air were significantly different (P&lt;0.01).

Sign in / Sign up

Export Citation Format

Share Document