scholarly journals EVALUATION OF METHODS FOR DETECTION OF COLIFORM BACTERIA IN RAW MILK1

1972 ◽  
Vol 35 (2) ◽  
pp. 67-70
Author(s):  
Jose de A. Moura Fé ◽  
F. Eugene Nelson
Keyword(s):  
Escherichia Coli ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Lauryl Sulfate ◽  
E Coli ◽  
Total Coliform Bacteria ◽  
Evaluation Of Methods

Estimates of populations of “total” coliform bacteria in raw mi1k were quantitatively essentially the same when Violet Red Bile agar incubated at 32 C and Lauryl Sulfate Tryptose Broth incubated at 37 C were used. Qualitatively, LST tended to favor recovery of a greater proportion of Aerobaoter aerogenes and IMViC-irregular types. Of the 46 isolates that produced ropiness, 35 were recovered from LST. Escherichia coli populations, as determined on EC medium incubated at 44.5 C, showed no constant relationship to total coliform population. Summer samples contained not only greater numbers of E. coli but also these organisms constituted a greater proportion of the coliform population.

ColiComplete® Substrate-Supporting Disc Method for Confirmed Detection of Total Coliforms and Escherichia coli in All Foods: Comparative Study

1994 ◽  
Vol 77 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Philip T Feldsine ◽  
Maria T Falbo-Nelson ◽  
David L Hustead
Keyword(s):  
Escherichia Coli ◽  
Comparative Study ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Total Coliforms ◽  
E Coli ◽  
Total Coliform Bacteria ◽  
Better Than

Abstract The ColiComplete® substrate-supporting disc (SSD) method for simultaneous confirmed total coliform count and Escherichia coli determination in all foods was compared with the AOAC most probable number (MPN) methods 966.23 and 966.24. In this comparative study, 20 water and food types were analyzed; 7 of these foods were naturally contaminated with coliform bacteria, 6 food types were naturally contaminated with E. coli, and the remaining foods were inoculated with coliform bacteria and/or E. coli. Data were analyzed separately for total coliform bacteria and for E. coli. Mean log MPN counts were determined by the SSD method and the appropriate AOAC MPN procedure. Results were then analyzed for mean log MPN differences and variance, according to methods described by AOAC INTERNATIONAL Results for both total conforms and E. coli indicate that the SSD method is equivalent to or better than AOAC MPN methods 966.23 and 966.24.

Automated Dead-End Ultrafiltration for Concentration and Recovery of Total Coliform Bacteria and Laboratory-Spiked Escherichia coli O157:H7 from 50-Liter Produce Washes To Enhance Detection by an Electrochemiluminescence Immunoassay

2013 ◽  
Vol 76 (7) ◽  
pp. 1152-1160 ◽  
Author(s):  
SONIA MAGAÑA ◽  
SARAH M. SCHLEMMER ◽  
STEPHANEY D. LESKINEN ◽  
ELIZABETH A. KEARNS ◽  
DANIEL V. LIM
Keyword(s):  
Escherichia Coli ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Dead End ◽  
Electrochemiluminescence Immunoassay ◽  
Low Levels ◽  
Total Coliform Bacteria

An automated concentration system (ACS) based on dead-end ultrafiltration was used in this study to concentrate bacteria, including Escherichia coli O157:H7, from 50-liter produce washes (PWs, sieved produce wash). Cells trapped in the filters were recovered in approximately 400 ml of buffer to create PW retentates (PWRs). Extent of concentration was determined by analyzing PWs and PWRs for total coliform bacteria and E. coli O157:H7 using standard methods. In addition, an electrochemiluminescence immunoassay was evaluated for detection of E. coli O157:H7 in spiked PWs and PWRs to demonstrate usefulness of the ACS for same-day detection. The levels of total coliform bacteria and E. coli O157:H7 in PWRs were higher than those in PWs by 1.85± 0.41 log most probable number per 100 ml and 1.82 ± 0.24 log CFU/ml, respectively. Electrochemiluminescence detection of E. coli O157:H7 was accomplished within 2 h using ACS concentration of lettuce and spinach wash water artificially spiked with the pathogen at levels as low as 0.36 log CFU/ml and 1.39 log CFU/ml, respectively. Detection of E. coli O157:H7 at −0.93 ± 0.15 log CFU/ml in lettuce wash occurred within approximately 6 h when a 4-h enrichment step was added to the procedure. Use of dead-end ultrafiltration increased bacterial concentrations in PWR and allowed same-day detection of low levels of E. coli O157:H7 in PW. This concentration system could be useful to improve the sensitivity of current rapid methods for detection of low levels of foodborne pathogens in PW water.

scholarly journals Assessment of microbial contamination of drinking water with total coliform bacteria and Escherichia coli in the Bitola region

2019 ◽  
Vol 65 (2) ◽  
pp. 23-32
Author(s):  
Marta Nedelkova ◽  
Angela Delova ◽  
Tanja Petreska Ivanovska ◽  
Zoran Zhivikj ◽  
Lidija Petrushevska-Tozi
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Supply ◽  
Rural Areas ◽  
Membrane Filtration ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Quality And Safety ◽  
E Coli ◽  
Total Coliform Bacteria

The aim of this paper is to analyze the presence of Escherichia coli (E. coli) and total coliform bacteria (CB) in the drinking water of urban and rural areas of the Bitola region, as indicators for water quality and safety. All water in urban area is chlorinated, while at the same time the water in rural areas is non-chlorinated. The samples were analyzed according to the international standard method of membrane filtration MKC EN ISO 9308-1:2015. In all examined samples of drinking water in urban area, presence of E. coli and CB was not detected as a result of the disinfection of the water. On contrary, in all tested samples of the water from the rural water supply, presence of E. coli and CB was confirmed. Significant increase in coliform bacterial counts probably weather-related was found in the period from April to September. In addition, in the third quarter including July, August, and September, in many measurements, E. coli as an indicator of faecal contamination was identified in drinking water. In accordance with these findings and in order to provide safe drinking water, it is necessary to modernize the water supply for the population in rural areas, to disinfect permanently the drinking water and to apply regular laboratory controls which are a basic pre-condition. Otherwise, inappropriate management of the water systems can cause serious decrease in the quality and safety of the drinking water associated with an increased risk of appearance of the infectious diseases in people and hydric epidemic. Key words: drinking water, Escherichia coli, coliform bacteria, membrane filtration

CONSUMPTION OF RAW WATER – THE HEALTH RISKS RELATED TO THE PRESENCE OF HEAVY METALS AND „ESCHERICHIA COLI“

2021 ◽  
Author(s):  
Mirjana Grujović ◽  
◽  
Katarina Mladenović ◽  
Zoran Simić ◽  
Simona Đuretanović
Keyword(s):  
Escherichia Coli ◽  
Trace Elements ◽  
Health Risks ◽  
Water Samples ◽  
Major And Trace Elements ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Mountain Stream ◽  
E Coli ◽  
Colorimetric Test

This research aimed to investigate the quality of drinking water from the rural area of village Pajsijević (Šumadija, central Serbia). The water is consumed as raw since it is not purified or chlorinated before consumption. The water was collected at three sampling points – in the spring of Kotlenik Mountain stream (W1 sample), in the local reservoir (W2 sample), and from the tap (W3 sample). Also, the sediment samples (soil and sand) were analyzed, too. The health risks related to the presence and concentration of some major and trace elements (Ca, Cr, Cu, Fe, Mg, Mn, Ni, Zn) and N, NO, NN3, NH4, P, P2O5, and PO4 were evaluated. Additionally, the presence and the number of total coliform bacteria and Escherichia coli (as an indicator of fecal contamination) were evaluated. The concentrations of analyzed major and trace elements in all water samples were below those at which toxic effects may occur. The exception was the concentrations of Fe (2.02 – 2012 mg/L), which were higher than is allowed. The origin of Fe in water is from sediment (soil and sand), which also showed high content of Fe (3006.0 mg/g and 2229.9 mg/g, respectively). The results of the Colorimetric test indicated the presence of coliform bacteria as well as the presence of E. coli in all water samples. Further research needs to include characterization of isolated coliform bacteria and serological investigation of E. coli strains in order to evaluate the risks of consumption related to waterborne illness.

scholarly journals A study in the experimental conditions of pesticide action on microorganisms characterizing sanitary-epidemiological safety of reservoirs

2019 ◽  
Vol 95 (8) ◽  
pp. 785-789 ◽  
Author(s):  
V. V. Aleshnya ◽  
Peter V. Zhuravlev ◽  
O. P. Panasovets
Keyword(s):  
River Water ◽  
Strain Differences ◽  
Water Source ◽  
Total Coliform ◽  
The Body ◽  
Coliform Bacteria ◽  
Experimental Conditions ◽  
E Coli ◽  
Pesticide Pollution ◽  
Total Coliform Bacteria

The paper presents experimental data on the pesticides action (molinate (OrdramA), Dinitroortocresolum and chlorophos) at concentrations of 0.01 mg /l to 10 mg/l on sanitary-indicative (TBC, total coliform bacteria, E. coli, E. faecalis), potentially pathogenic ( Ps. aeruginosa) and pathogenic (S. Derby) microflora of the river water. As the test cultures there were used isolated from the reservoir and museum strains of microorganisms. Due to the fact that the rate of decomposition ofpesticides in water increases in direct proportion to the extent of its biological contamination, the experiments were performed with the river water: native and sterilized by autoclaving. There were identified species and strain differences in the microbial response to the action of pesticides. The selectivity of OrdramA action at a concentration of 0.1 to 10 mg/l on Salmonella and of chlorophos at a concentration of 10 mg/l for Salmonella and E. coli is manifested in the stimulation of the reproduction of these bacteria. Dinitroortocresolum in concentrations of 10 mg/l and 1 mg/l gives rise in the reproduction of Pseudomonas aeruginosa and inhibits the growth of total coliform bacteria and E. coli, in a concentration of 0.1 mg/l the preparation stimulates the viability of all the studied microorganisms. When entering the body of water, pesticides were found to cause changes of biocenosis in the river water and disturb bacterial self-cleaning processes. In conditions of pesticide pollution total coliform bacteria, E. coli and E. faecalis unable to maintain their indicative value and therefore the use of only them for the evaluation of the sanitary-epidemiological status of the water source appears to be insufficient. Due to the fact that the epidemic potential of water is directly dependent on quantitative content ofpathogenic and opportunistic pathogenic microorganisms in it, the reproduction Salmonella and Pseudomonas bacteria is a negative moment from the sanitary and epidemiological point of view.

scholarly journals Evaluation of X-GAL-MUG Test for the Detection of Total Coliform Bacteria and Escherichia coli in Drinking Water.

1996 ◽  
Vol 13 (2) ◽  
pp. 63-67
Author(s):  
Hiroshi NAKAYAMA ◽  
Kazumi HORIKAWA ◽  
Takeshi USHIO ◽  
Tastuoki MASTUNAGA ◽  
Masatoshi YAIRI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Total Coliform Bacteria

scholarly journals Impacts of Intermittent Water Supply on Water Quality in Two Palestinian Refugee Camps

Water ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 670 ◽  
Author(s):  
Shatha Alazzeh ◽  
Stephanie Galaitsi ◽  
Amahl Bishara ◽  
Nidal Al-Azraq ◽  
John L. Durant
Keyword(s):  
Water Quality ◽  
Water Supply ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Residual Chlorine ◽  
Refugee Camps ◽  
E Coli ◽  
Palestinian Refugee ◽  
Treated Drinking Water ◽  
Total Coliform Bacteria

We investigated the quality of treated drinking water in Aida Camp and Alazzah Camp, two Palestinian refugee camps in the West Bank. Water supply in the two camps is intermittent: Water deliveries are first stored in a centrally located community tank before being pumped through distribution networks to users, who then store the water in rooftop tanks. Between March 2016 and June 2017, we examined water quality within the distribution system in the camps, testing 520 samples in Aida Camp and 198 samples in Alazzah Camp for total coliforms, Escherichia coli (E. coli) bacteria, and residual chlorine. We observed deterioration in water quality in both camps, but the deterioration was more pronounced in Aida Camp where 2.5% of community-tank samples, 5.0% of network samples, and 10% of rooftop-tank samples contained one or more colonies (per 100 mL) of total coliform bacteria. In Alazzah Camp, 3.7% of community-tank samples, 2.5% of network samples, and 5% rooftop-tank samples contained one or more colonies (per 100 mL) of total coliform bacteria. E. coli was detected in two samples from Aida Camp and one sample from Alazzah Camp. In both camps, average residual chlorine concentrations were higher in the community tanks than in rooftop tanks. The main factor influencing water quality deterioration in rooftop tanks in Aida Camp appears to be air temperature. We recommend more systematic water testing in the camps and greater transparency in reporting test results to camp residents.

scholarly journals Early detection of E. coli and total coliform using an automated, colorimetric and fluorometric fiber optics-based device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (17) ◽  
pp. 2925-2935 ◽  
Author(s):  
Sabiha Tok ◽  
Kevin de Haan ◽  
Derek Tseng ◽  
Can Firat Usanmaz ◽  
Hatice Ceylan Koydemir ◽  
...  
Keyword(s):  
Early Detection ◽  
Fiber Optics ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
E Coli ◽  
Total Coliform Bacteria

We report early detection of E. coli and total coliform bacteria using an automated, colorimetric and fluorometric fiber optics-based device.

Microbial Quality of Bagged Baby Spinach and Romaine Lettuce: Effects of Top versus Bottom Sampling

2012 ◽  
Vol 75 (1) ◽  
pp. 132-136 ◽  
Author(s):  
JULIE A. KASE ◽  
STACEY BORENSTEIN ◽  
ROBERT J. BLODGETT ◽  
PETER C. H. FENG
Keyword(s):  
Escherichia Coli ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Microbial Quality ◽  
Romaine Lettuce ◽  
Probable Number ◽  
E Coli ◽  
Bottom Sampling

Contamination with Escherichia coli O157:H7 and Salmonella have called into question the safety and microbial quality of bagged ready-to-eat leafy greens. This study expands on previous findings that these goods have high total bacteria counts (TBC) and coliform counts, variation in counts among different lots, that Escherichia coli is present, and disparities in counts when bags are top or bottom sampled. Nearly 100 bags of baby spinach and hearts of romaine lettuce from a single brand were subjected to both top and bottom sampling. Product was blended, and a portion serially diluted and plated to obtain TBC. Total coliform and E. coli levels were estimated by the most-probable-number (MPN) technique with ColiComplete discs. Top-sampled TBC from bags of baby spinach (48 bags, 13 different lots) ranged from 3.9 to 8.1 log CFU/g and bottom-sampled TBC ranged from 4.0 to 8.2 log CFU/g, with 52% of the bags (or 39% of the lots) producing TBC higher in bottom samples. For hearts of romaine (47 bags from 19 different lots), top-sampled bags had TBC ranging from 2.4 to 7.0 log, and bottom-sampled bags had TBC from 3.3 to 7.3 log, with 64% of the bags (or 63% of the lots) showing higher TBC in bottom samples. However, we are unable to reject the hypothesis that the top and bottom samples from either commodity contain the same TBC (P ≥ 0.08). No E. coli was detected and total coliform bacteria counts were, with few exceptions, ≥210 MPN/g, irrespective of TBC. In general, lots with the most number of days before the printed “use-by” date had lower TBC. However, the R2 values for either baby spinach (0.4085) or hearts of romaine (0.2946) suggest that age might not be a very good predictor of higher TBC. TBC varied widely between lots and even more so within same-lot samples, as indicated by the sum of squares results. This finding, along with higher TBC in bottom samples, suggests further consideration when a microbiological sampling scheme of bagged produce is designed.

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