Prevalence of Listeria spp. in Poultry Meat at the Supermarket and Slaughterhouse Level

1989 ◽  
Vol 52 (9) ◽  
pp. 618-624 ◽  
Author(s):  
CONSTANTIN A. GENIGEORGIS ◽  
DAN DUTULESCU ◽  
J. FERNANDEZ GARAYZABAL
Keyword(s):  
Large Intestine ◽  
Poultry Meat ◽  
National Brands ◽  
Drip Water ◽  
Poultry Products ◽  
High Prevalence ◽  
Processing Line ◽  
Meat Samples ◽  
Water Overflow ◽  
Tank Water

The prevalence of Listeria spp. in the skins of poultry legs (drumsticks), wings, and whole livers, representing 160 packages, three national brands, and purchased from three supermarkets in Davis, California was investigated first. Overall, Listeria spp., L. monocytogenes, L. innocua, and L. welshimeri were present in 40.6, 13.1, 26.3, and 1.3% of the chicken parts. For L. monocytogenes spp. the overall prevalence in the skins of wings, drumsticks, and livers was 10, 15, and 14% and varied extensively with sampling day. Next, we evaluated the presence of Listeria spp. in 12 locations and products collected from a slaughterhouse during four visits. A total of 188 samples were analyzed. No Listeria spp. were isolated from feathers (5 g composite sample from 10 birds), scalding tank water overflow (25 ml), chiller incoming water (25 ml), neck skin (5–9 g), whole liver after chilling, and cecum and large intestine content (1 g) samples. Listeria spp. were present in 18.8% of feather picker drip water (25 ml), 12.5% of chiller water overflow (25 ml), 37.5% of recycling water for cleaning gutters (25 ml), 25% of hearts, and 31.3% of mechanically deboned meat samples (25 g). The prevalence of L. monocytogenes in packaged livers and skins of drumsticks and wings at the end of the processing line was 33.3, 36.7, and 70.0%, respectively. After 4 d storage of the same packages at 4°C, L. monocytogenes was recovered from 40, 52, and 72% of the respective products. The prevalence of L. monocytogenes on the hands and gloves of the persons hanging birds after chilling, cutting carcasses, and packaging parts was 20, 45.5, and 59%. respectively. Overall, the study demonstrated the high prevalence of Listeria spp. and L. monocytogenes in poultry products and, that through certain improvements and innovations at the slaughterhouse level, contamination with Listeria can be minimized.

Prevalence of Listeria spp. in Turkey Meat at the Supermarket and Slaughterhouse Level

1990 ◽  
Vol 53 (4) ◽  
pp. 282-288 ◽  
Author(s):  
CONSTANTIN A. GENIGEORGIS ◽  
PETRU OANCA ◽  
DAN DUTULESCU
Keyword(s):  
Large Intestine ◽  
National Brands ◽  
Drip Water ◽  
Turkey Meat ◽  
High Prevalence ◽  
Water Overflow ◽  
Tank Water ◽  
Composite Samples

The prevalence of Listeria spp. in the skins of turkey wings, legs (drumsticks), and tails was studied first. During three trips to local supermarkets we purchased and analyzed 180 packages representing two national brands. Overall, total Listeria spp., L. monocytogenes, L. welshimeri, and L. innocua were present in 32.2, 15.0, 15.6, and 1.7% of the packages. Listeria spp. were present in 23.3 and 41.1% of the products of Company A and B, respectively. The corresponding figures for L. monocytogenes were 12.2 and 17.8% and for L. welshimeri, 11.1 and 20.0%. The overall prevalence of Listeria spp. on wings, legs, and tails was 45.0, 28.5, and 23.3%, respectively. The corresponding numbers for L. monocytogenes were 20.0, 13.3, 11.7%. Next, we determined the presence of Listeria spp. in 10 locations and products collected from a slaughterhouse of Company A during three visits. A total of 225 samples were analyzed. No Listeria spp. were isolated from 30 feather samples (5g composite samples from 10 birds), 15 scalding tank water overflow samples (25 ml), 30 samples of neck skin (149 to 183 cm2), 30 whole liver and 30 heart samples after chilling, and 30 samples of cecum and large intestine content (1g). Listeria spp. were present in 13.4, 6.7, 33.3, and 26.7% of 15 feather picker drip water (25 ml), 15 chiller water overflow (25 ml), 15 recycling water for cleaning gutters (25 ml), and in 15 of mechanically deboned meat (25 g) samples, respectively. The prevalence of Listeria spp. in wings, legs, and tails increased through processing and distribution. The prevalence immediately after chilling, after packaging and at the retail level was 4.4, 13.3, and 23.3%. No Listeria spp. was isolated from 30 livers after chilling and 30 liver packages ready to go to the market. The prevalence of Listeria spp. on the hands and gloves of the persons hanging birds after chilling, cutting carcasses, and packaging parts was 16.7, 33.3, and 40.0%, respectively. Overall, the study demonstrated the high prevalence of Listeria spp. and specifically L. monocytogenes in turkey products. Improvements and innovations at the slaughterhouse level may effectively reduce final product contamination with Listeria.

β-Resorcylic Acid, a Phytophenolic Compound, Reduces Campylobacter jejuni in Postharvest Poultry

2017 ◽  
Vol 80 (8) ◽  
pp. 1243-1251 ◽  
Author(s):  
B. R. Wagle ◽  
K. Arsi ◽  
A. Upadhyay ◽  
S. Shrestha ◽  
K. Venkitanarayanan ◽  
...  
Keyword(s):  
Breast Meat ◽  
Stress Response Genes ◽  
Poultry Products ◽  
Significant Difference ◽  
High Prevalence ◽  
Regulated Expression ◽  
Chicken Skin ◽  
Wild Strains ◽  
Poultry Processing ◽  
Meat Samples

ABSTRACTHuman Campylobacter infections, a leading foodborne illness globally, has been linked with the high prevalence of this bacterium on raw retail chicken products. Reduction of Campylobacter counts on poultry products would greatly reduce the risk of subsequent infections in humans. To this end, this study investigated the potential of the phytophenolic compound β-resorcylic acid (BR) to reduce Campylobacter counts on postharvest poultry (chicken skin or meat). Four trials in total, two each on thigh skin or breast meat, were conducted in which chicken skin or meat samples (2 ± 0.1 g; 10 samples per treatment) were inoculated with 50 μL (∼106 CFU per sample) of a cocktail of four wild strains of C. jejuni. After 30 min of attachment, inoculated samples were dipped in a 0, 0.5, 1, or 2% BR solution for 30 s immediately followed by vigorously vortexing the samples in Butterfield's phosphate diluent and plating the supernatant for Campylobacter enumeration. In addition, the effect of BR on the color of skin and meat samples was studied. Moreover, the change in the expression of survival and virulence genes of C. jejuni exposed to BR was evaluated. Data were analyzed by the PROC MIXED procedure of SAS (P < 0.05; SAS Institute Inc., Cary, NC). All BR treatments significantly reduced Campylobacter populations on both chicken or meat samples by 1 to 3 log CFU/g compared with non–BR-treated washed controls. No significant difference in the lightness, redness, and yellowness of skin and meat samples was observed on exposure to BR wash (P > 0.05). Real-time PCR results revealed that BR treatment down-regulated expression of select genes coding for motility (motA, motB) and attachment (cadF, ciaB) in the majority of C. jejuni strains. Stress response genes (sodB, katA) were upregulated in C. jejuni S-8 (P < 0.05). Overall, our results suggest that BR could be effectively used as antimicrobial dip treatment during poultry processing for reducing Campylobacter on chicken carcasses.

scholarly journals Broad Spectrum Detection of Antibiotic Residues in Poultry Meat by a Multi-Plate Assay

2019 ◽  
Vol 63 (3) ◽  
pp. 9-17 ◽  
Author(s):  
H. S. Watkins ◽  
I. Kožárová
Keyword(s):  
Broad Spectrum ◽  
Low Cost ◽  
Positive Sample ◽  
Poultry Meat ◽  
Antibiotic Residues ◽  
Poultry Products ◽  
Negative Results ◽  
Spectrum Detection ◽  
The Matrix ◽  
Meat Samples

Abstract The objective of this study was to use the “Screening test for antibiotic residues” (STAR) as a broad-spectrum detection method for antibiotic residues in poultry meat. The STAR method is a microbiological inhibition assay (a five plate test) where the development of inhibition zones (IZs) indicates the presence of antibiotic residues in meat samples. By using the STAR method, in a total of 13 poultry products providing 18 meat samples (14 muscle and 4 skin) and 18 corresponding juice samples, 11 out of the 18 samples were positive for containing antibiotic residues. Based on muscle alone (which is the matrix validated for use in the STAR method), 6 of the 14 muscle samples were positive for antibiotic residues. The STAR method as a screening technique proves advantageous as it is relatively easy to perform and of a low cost. Furthermore, the STAR method not only indicates the presence or absence of antibacterial substances, but simultaneously, a positive sample gives an indication of the antibiotic family present due to the use of five different bacterial test organisms. Families of antibiotics pre-identified due to positive samples in the results of this study include aminoglycosides (one out of 18), beta-lactams and sulphonamides (6 out of 18), and macrolides (5 out of 18). Such pre-identification of the antimicrobial families allows for a targeted confirmatory analysis. However, one could argue that the STAR method is laborious and time consuming. Overall, given the potential for false positive/negative results, further confirmatory method analysis of the samples must be performed to ensure that the results and conclusions drawn here are true.

Atmospheric Cold Plasma and Peracetic Acid–Based Hurdle Intervention To Reduce Salmonella on Raw Poultry Meat

2019 ◽  
Vol 82 (5) ◽  
pp. 878-888 ◽  
Author(s):  
SHREYAK CHAPLOT ◽  
BARUN YADAV ◽  
BYEONGHWA JEON ◽  
M. S. ROOPESH
Keyword(s):  
Salmonella Typhimurium ◽  
Peracetic Acid ◽  
Cold Plasma ◽  
Large Scale ◽  
Antimicrobial Agents ◽  
Synergetic Effect ◽  
Poultry Meat ◽  
Poultry Products ◽  
Meat Samples ◽  
Raw Poultry

ABSTRACT In Canada, Salmonella-related foodborne illness accounts for more than 88,000 cases annually. Poultry products represent one of the major vectors for the zoonotic transmission of Salmonella. The majority of the current disinfection strategies that are applied in the poultry industry involve the use of diverse chemical antimicrobial agents; however, knowledge about the efficacy of novel antimicrobial technologies such as atmospheric cold plasma (ACP) and the potential of hurdle interventions is very limited. The objective of this study was to evaluate the synergetic effect of ACP and peracetic acid (PAA) as a hurdle antimicrobial intervention to reduce Salmonella enterica Typhimurium on raw poultry meat. Raw poultry meat samples were inoculated with Salmonella Typhimurium followed by the application of different treatments consisting of ACP and PAA (100 and 200 ppm) alone as well as in combination. Different hurdle interventions using PAA and ACP treatments resulted in significant (P ≤ 0.05) reductions in Salmonella Typhimurium, ranging from 2.3 to 5.3 log CFU/cm2, in comparison to PAA treatments alone with 100 or 200 ppm or ACP treatment alone, resulting in the reduction of Salmonella populations by 0.6, 1.3, and 2.3 CFU/cm2, respectively. Treatments involving application of PAA followed immediately by ACP and ACP followed by PAA resulted in the highest (P ≤ 0.05) reduction in Salmonella by 4.7 and 5.3 log CFU/cm2, respectively. Transmission electron microscopy images indicated that combined treatments resulted in destruction of Salmonella cells with visible cellular deformation and loss of cellular integrity. Color and moisture content of poultry meat samples were affected; thus, for large-scale application, further research needs to be done for optimizing this hurdle intervention. In conclusion, this study demonstrates the synergistic effect of ACP and PAA and its potential application for the safety of poultry products. HIGHLIGHTS

scholarly journals Poultry: A receptacle for non-typhoidal Salmonellae and antimicrobial resistance

2019 ◽  
Author(s):  
Sohan Rodney Bangera ◽  
Shashikiran Umakanth ◽  
Goutam Chowdhury ◽  
Rudra Narayan Saha ◽  
Asish K. Mukhopadhyay ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Virulence Gene ◽  
Cross Sectional Study ◽  
Poultry Meat ◽  
Cross Sectional ◽  
Inva Gene ◽  
Medical College ◽  
Poultry Products ◽  
Hygienic Measures ◽  
Meat Samples

Background and Objectives: Non-typhoidal Salmonellosis, a zoonotic infection associated with acute gastroenteritis is caused by non-typhoidal salmonellae (NTS). The study was carried out to determine the prevalence of NTS serovars and their antimicrobial resistance along with the presence of the virulence gene (invA gene)  in poultry samples. Materials and Methods: This is a prospective cross-sectional study carried out at the Enteric Diseases Division, Kasturba Medical College, Manipal, South India from January 2016– December 2017. Poultry samples were collected randomly from two local poultry farms in Udupi district and processed following CDC standard protocol. Results: From the 396 poultry meat samples, intestinal contents and faecal samples collected, 58 NTS serovars were isolated showing a prevalence of 14.64%. Salmonella Infantis, 43.1%, 25/58 was the commonest serovar. Resistance to ciprofloxacin 72.41%, ampicillin 32.8%, gentamicin 17.24%, cotrimoxazole 29.31% and amoxicillin-clavulanic acid 6.9% was observed. The invA gene was detected in 43 NTS isolates (74.13%). Conclusion: Poultry sources are recognized as a significant cause for non-typhoidal salmonellosis. Therefore, hygienic measures should be initiated to reduce the contamination of meat and poultry products with virulent strains of Salmonella that are of public health significance.

scholarly journals Bacteriological assessments of foodborne pathogens in poultry meat at different super shops in Dhaka, Bangladesh

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Jalal Uddin ◽  
Khaled Hossain ◽  
Saddam Hossain ◽  
Karabi Saha ◽  
Fatema Tuz Jubyda ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Culture Method ◽  
Poultry Meat ◽  
Salmonella Spp ◽  
E Coli ◽  
Poultry Products ◽  
Enteropathogenic Bacteria ◽  
Conventional Culture ◽  
Shigella Spp ◽  
Meat Samples

Poultry is now considered as a major fast-growing source of meat in the world. The consumers demand safe and hygienic products without contamination with pathogenic microorganisms when the production and consumption of poultry meat is gradually increasing. The present study was conducted to assess the bacterial contamination of dressed chicken collected from different supershops in Dhaka, Bangladesh. The chicken samples from S1, S2, M1, M2 and A supershops were analyzed to determine the enteropathogenic bacteria in poultry meat. Three genera of bacteria were isolated from all of the chicken meat samples. These enteropathogens from various organs of dressing chickens were also enumerated. The isolates were presumptively identified as E. coli, Salmonella spp., and Shigella spp. by conventional culture method. The three enteropathogens were subjected to PCR assay for their confirmation as virulent enteropathogens. Only E. coli isolates were confirmed as pathogenic E. coli (Enterotoxigenic), other isolates were not confirmed as virulent Salmonella spp., Shigella spp.. Results of this study demonstrated that more cautions are recommended for personnel hygiene in processing and handling of poultry and poultry products to prevent occurrence of enterotoxigenic E. coli in dressed poultry meat sold by the supershops in Bangladesh.

scholarly journals Enrichment Free qPCR for Rapid Identification and Quantification of Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis in Chicken Meat Samples by a New Couple of Primers

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2341
Author(s):  
Priya Vizzini ◽  
Jasmina Vidic ◽  
Marisa Manzano
Keyword(s):  
Campylobacter Jejuni ◽  
Limit Of Detection ◽  
Rapid Identification ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Rrna Genes ◽  
23S Rrna ◽  
Poultry Products ◽  
Pure Cultures ◽  
Meat Samples

Campylobacter is the main cause of bacterial foodborne disease and poultry meat is the principal source of human infections. Rapid methods for Campylobacter detection are urgently needed to decrease high bacterial prevalence in poultry products. In this study, we developed new primers, CampyPFw and CampyPRv, that target the 16S-23S rRNA genes of Campylobacter jejuni, C. coli, C. lari and C. upsaliensis. The primers were tested on positive and negative reference strains in pure cultures and in inoculated poultry meat samples before their application in real-time PCR (qPCR) protocol for analyzing chicken meat samples. In parallel, the samples were tested by using the ISO 10272-1:2006 method. The qPCR protocol based on CampyPFw and CampyPRv showed good sensitivity, with the limit of detection of 4.6 × 102 cells/mL in chicken samples without enrichment steps.

Identification of antibiotics in meat. Flow cytometry capabilities

Vsyo o myase ◽  
2020 ◽  
pp. 56-60
Author(s):  
Zayko E.V. ◽  
◽  
Kuznetsova O.A. ◽  
Bataeva D.S. ◽  
Grudistova M.A. ◽  
...  
Keyword(s):  
Raw Materials ◽  
Animal Husbandry ◽  
Flow Cytometer ◽  
Poultry Meat ◽  
Meat Processing ◽  
Beta Lactams ◽  
Antimicrobial Substances ◽  
Chemotherapy Drugs ◽  
Processing Plants ◽  
Meat Samples

The problem of the uncontrolled use of antibiotics currently remains unresolved. Step-by-step monitoring of meat using modern methods will reduce the risk of using contaminated meat raw materials for food production. Qualitative monitoring will identify samples containing residual antimicrobial substances. The use of methods for identifying groups of antibiotics will help narrow the search for antibiotics by expensive chromatographic methods. A study was carried out of beef, pork and poultry meat, which is used in meat processing plants in the production of raw smoked sausages, using two methods. At the first stage, using a qualitative microbiological method, the raw meat was evaluated for the presence of antimicrobial substances, then their group was determined using a NovoCyte flow cytometer. According to the results of a study on a flow cytometer, it was found that out of 10 groups of antibiotics that can be determined by the tested method, the group of lincosamides was not found in all meat samples. The most common groups of chemotherapy drugs in pork were sulfonamides – 29.6 %, tetracycline group – 18.5 % and beta-lactams – 14.8 %, and in beef aminoglycosides – 36.7 %, phenicols – 30 % and beta-lactams – 13.3 %. In poultry meat samples, the most common were sulfonamides – 23.2 %, fenicols – 23.2 %, and beta-lactams – 16 %. Five groups of antibiotics were found in all studied types of meat: fenicols, β-lactams, macrolides, polypeptide antibiotics, and quinolones. This indicates their widespread use in animal husbandry and poultry farming.

scholarly journals High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil

2007 ◽  
Vol 79 (1) ◽  
pp. 111-114 ◽  
Author(s):  
RN Belfort ◽  
Veronique Nussenblatt ◽  
Luiz Rizzo ◽  
Cristina Muccioli ◽  
Claudio Silveira ◽  
...  
Keyword(s):  
Restriction Analysis ◽  
Rflp Analysis ◽  
Type I ◽  
Type Ii ◽  
Molecular Biological Technique ◽  
High Prevalence ◽  
Biological Technique ◽  
Infectious Uveitis ◽  
Toxoplasma Gondii Infection ◽  
Meat Samples

Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.

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