Growth and Attachment of Listeria monocytogenes to Cast Iron

1991 ◽  
Vol 54 (12) ◽  
pp. 925-929 ◽  
Author(s):  
ALEXANDRA T. SPURLOCK ◽  
E. A. ZOTTOLA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Stainless Steel ◽  
Cast Iron ◽  
Listeria Monocytogenes ◽  
Growth Period ◽  
Iron Surface ◽  
Free Standing ◽  
Steel Surfaces ◽  
Scanning Electron

The growth and potential attachment of Listeria monocytogenes to cast iron in drains was investigated in this study. L. monocytogenes was grown in rich and dilute nutritive media in free-standing cast iron drains. The pH in the drain was adjusted over the growth period to pH 4.5, 7.0, and 9.0. L. monocytogenes was found to survive pH adjustments in drains for 28 d. Scanning electron microscopy (SEM) was used to investigate the attachment of L. monocytogenes to cast iron chips. SEM observation showed L. monocytogenes cells apparently attached to the iron surface of the chip. Listeria does not appear to attach as readily to cast iron as to stainless steel surfaces.

scholarly journals Scanning electron microscopy of Listeria monocytogenes biofilms on stainless steel surfaces

2009 ◽  
Vol 59 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Milanov Dubravka ◽  
Asanin Ruzica ◽  
Vidic Branka ◽  
Krnjaic D. ◽  
Petrovic Jelena ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Steel Surfaces ◽  
Scanning Electron

Formation of Biofilms by Listeria monocytogenes under Various Growth Conditions

2005 ◽  
Vol 68 (1) ◽  
pp. 92-97 ◽  
Author(s):  
ANDREW G. MOLTZ ◽  
SCOTT E. MARTIN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Cell Density ◽  
Polyvinyl Chloride ◽  
Growth Medium ◽  
Biofilm Formation ◽  
Growth Conditions ◽  
Scanning Electron

Eight strains of Listeria monocytogenes (7644, 19112, 15313, Scott A, LCDC, 10403S, SLCC, and 1370) produce biofilms when grown on polyvinyl chloride microtiter well plates. The growth medium (tryptic soy broth [TSB] or modified Welshimer's broth [MWB] at 32°C) influenced the amount of biofilm formed; maximum biofilms were formed in MWB by six strains and in TSB by the remaining two strains. This result suggests that the growth medium is critical in development of L. monocytogenes biofilm. This organism also produced biofilms on stainless steel chips. Biofilm formation on these chips was observed following growth in TSB at 4, 20, and 37°C. After 20 h of incubation at 20 or 37°C, the cell density was approximately 106 CFU per chip, and after 4 days incubation at 4°C, the cell density was 105 CFU per chip. L. monocytogenes strain Scott A biofilm formation on stainless steel chips was visualized using scanning electron microscopy, which revealed dense aggregates of cells held together by meshlike webbing.

Analyse of Indium Loss in Preparation of CuInSe2 Flims for Solar Cells

2011 ◽  
Vol 183-185 ◽  
pp. 1837-1841
Author(s):  
Lei Sha ◽  
Yan Lai Wang ◽  
Shi Liang Ban
Keyword(s):  
Electron Microscopy ◽  
Thin Films ◽  
Scanning Electron Microscopy ◽  
Stainless Steel ◽  
Solar Cells ◽  
Surface Morphology ◽  
Energy Dispersive Spectroscopy ◽  
Titanium Substrates ◽  
Selenization Process ◽  
Scanning Electron

CuInSe2 thin films were obtained by selenization of the Cu-In precursors in the atmosphere of Se vapour, which were prepared on stainless steel and titanium substrates by electrodeposition. The films were characterized by XRD, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The respective influences of composition, phases and surface morphology of Cu-In precursors on indium loss were investigated. The results indicate that the indium loss occurs in selenization process because of volatile In2Se arising. The indium loss is less in selenization process of Cu-In precursors contained CuIn, Cu2In and In phases.

Research on the Adhesion of Stainless Steel Film on Gd by Magnetron Sputtering

2011 ◽  
Vol 189-193 ◽  
pp. 129-136
Author(s):  
Xiao Qiu Zheng ◽  
Shi Kun Xie ◽  
Rong Xi Yi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Stainless Steel ◽  
Magnetron Sputtering ◽  
Protective Coating ◽  
Tension Test ◽  
Dc Magnetron Sputtering ◽  
Thin Fiber ◽  
Scanning Electron ◽  
Strength Of Adhesion

In order to research the adhesion of sputtering protective coating of Gd. Gd substrates was coated with 1Cr18Ni9Ti by means of DC magnetron sputtering technology. The characteristics of the film were investigated by scanning electron microscopy (SEM), EDS, SPM and the adhesions of film was tested by tension test. The results show that the films of 1Cr18Ni9Ti are distributed by means of islands when the sputtering was initiated and the grains are like thin fiber. After a few minutes, the films are smooth and perfect, the interferences between 1Cr18Ni9Ti and Gd join together strongly, and the largest strength of adhesion is 24.7MPa when the sputtering density is 966 w/cm2 and the sputtering time is 8 minutes.

Scanning Electron Microscopy of Microbial Attachment to Milk Contact Surfaces1

1981 ◽  
Vol 44 (3) ◽  
pp. 204-208 ◽  
Author(s):  
P. T. ZOLTAI ◽  
E. A. ZOTTOLA ◽  
L. L. MCKAY
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Stainless Steel ◽  
Fibrous Material ◽  
Microbial Colonization ◽  
Lactobacillus Bulgaricus ◽  
Stainless Steel Surface ◽  
Pseudomonas Fragi ◽  
Scanning Electron ◽  
Microbial Attachment

Milk contact surfaces were observed by scanning electron microscopy (SEM) techniques for possible microbial attachment. Cultures of Pseudomonas fragi 4973, Staphylococcus aureus JAL, Streptococcus lactis C2, Streptococcus cremoris and Lactobacillus bulgaricus RR inoculated onto glass coverslips or stainless steel chips were examined. Stainless steel surfaces displayed many possible harborages for microbial colonization. SEM examination of P. fragi 4973 showed development of fibrous material, with numerous stick-like projections extending from the cell to the glass or stainless steel surface. These apparent attachment appendages became more pronounced as contact time increased. S. aureus, S. lactis, S. cremoris and L. bulgaricus did not display such fibrous material.

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