Immunochemical Detection of Molds: A Review

2000 ◽  
Vol 63 (2) ◽  
pp. 281-291 ◽  
Author(s):  
SUZHEN LI ◽  
R. R. MARQUARDT ◽  
D. ABRAMSON
Keyword(s):  
Polyclonal Antibodies ◽  
Fusarium Species ◽  
Animal Health ◽  
Water Soluble ◽  
Heat Stable ◽  
Regulatory Strategies ◽  
Highly Sensitive ◽  
Wide Range ◽  
Immunochemical Detection ◽  
Food And Feed

Molds are widely distributed in nature and cause deterioration of foods and feeds. Their mycotoxins can adversely affect human and animal health. Suitable assays for molds, therefore, are required to implement control and regulatory strategies and to develop appropriate feeding regimens for mold-infested feeds. Many different types of mold assays have been used, most of which are not reproducible or accurate. However, the immunoassays, particularly enzyme-linked immunosorbent assays (ELISAs), can be especially useful. Among these, assays that detect the water-soluble extracellular secretions of fungi, the exoantigens, are generally able to detect fungi at the genus or species level, whereas the heat-stable polysaccharides tend to be specific for one or more genus of fungi. Several species and genus (genera)–specific ELISAs have been developed using monoclonal or polyclonal antibodies against exoantigens and heat-stable polysaccharides from a wide range of fungi, including Aspergillus, Penicillium, and Fusarium species. Other assays have been developed that nonspecifically detect mold in food or feed, some using antibodies against a mixture of antigens from different fungi. These assays are highly sensitive, are easy to perform, and provide an index of the amount of mold present in the sample. Further refinement of these assays should facilitate their widespread use by food and feed processors, regulatory agencies, taxonomists, and research scientists.

scholarly journals Isolation, Molecular Identification and Mycotoxin Profile of Fusarium Species Isolated from Maize Kernels in Iran

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 297 ◽  
Author(s):  
Maryam Fallahi ◽  
Hossein Saremi ◽  
Mohammad Javan-Nikkhah ◽  
Stefania Somma ◽  
Miriam Haidukowski ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Fusarium Species ◽  
Animal Health ◽  
Elongation Factor ◽  
Ear Rot ◽  
Translation Elongation ◽  
Wide Range ◽  
Severe Reduction ◽  
Maize Kernels ◽  
High Possibility

Fusarium species are among the most important fungal pathogens of maize, where they cause severe reduction of yield and accumulation of a wide range of harmful mycotoxins in the kernels. In order to identify the Fusarium species and their mycotoxin profiles associated to maize ear rot and kernel contamination in Iran, a wide sampling was carried out from field in ten major maize-producing provinces in Iran, during 2015 and 2016. From 182 samples of maize kernels, 551 strains were isolated and identified as belonging to Fusarium genus. Among the 234 representative strains identified at species level by translation elongation factor (EF-1α) sequences, the main Fusarium species were F. verticillioides and F. proliferatum, together representing 90% of the Iranian Fusarium population, and, to a lesser extent, F. incarnatum equiseti species complex (FIESC), F. thapsinum and F. redolens. Fumonisin (FBs) production by F. verticillioides and F. proliferatum representative strains was analysed, showing that all strains produced FB1. None of F. verticillioides strains produced FB2 nor FB3, while both FB2 and FB3 were produced only by F. proliferatum. Total mean of FBs production by F. verticillioides was higher than F. proliferatum. The occurrence of different Fusarium species on Iranian maize is reason of great concern because of the toxigenic risk associated to these species. Moreover, the diversity of the species identified increases the toxigenic risk associated to Fusarium contaminated maize kernels, because of the high possibility that a multi-toxin contamination can occur with harmful consequences on human and animal health.

What about the 'other' Fusarium mycotoxins?

2009 ◽  
Vol 2 (2) ◽  
pp. 181-192 ◽  
Author(s):  
M. Jestoi ◽  
M. Kokkonen ◽  
S. Uhlig
Keyword(s):  
Current Knowledge ◽  
Fusarium Species ◽  
Animal Health ◽  
Natural Occurrence ◽  
Fusarium Mycotoxins ◽  
Fusarium Spp ◽  
Natural Toxins ◽  
Toxic Metabolites ◽  
Food And Feed ◽  
Very High

Most Fusarium species are capable of producing mycotoxins that may cause adverse effects on human or animal health. The most commonly studied Fusarium mycotoxins include trichothecenes, zearalenone and fumonisins. However, it seems that nearly all of the most prevalent Fusarium species infecting grains are also capable of producing other toxic metabolites. The existing studies, although exiguous, have clearly demonstrated that other toxic metabolites of Fusarium spp. are also present in our foods and feeds, occasionally at very high levels. It is apparent that since mycotoxins, including these 'other' metabolites, are natural toxins, they cannot be completely eliminated from food and feed chains. However, scientific studies are needed to determine their true significance. Thus, the mechanism and level of toxicity as well as presence and concentration levels will have to be fully clarified. In this paper, we briefly review the prevalence of the dominant Fusarium species contaminating maize and small-grain cereals worldwide, and the current knowledge on the biological activity as well as the natural occurrence of their selected less-known toxic metabolites. Additionally, the significance of these 'other' Fusarium mycotoxins is discussed.

scholarly journals Preliminary Study on the Use of Chitosan as an Eco-Friendly Alternative to Control Fusarium Growth and Mycotoxin Production on Maize and Wheat

Pathogens ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 29 ◽  
Author(s):  
Vanessa Zachetti ◽  
Eugenia Cendoya ◽  
María Nichea ◽  
Sofía Chulze ◽  
María Ramirez
Keyword(s):  
Growth Rates ◽  
Fusarium Species ◽  
Animal Health ◽  
Low Molecular Weight ◽  
Head Blight ◽  
Mycotoxin Production ◽  
Low Molecular Weight Chitosan ◽  
Food And Feed ◽  
Preliminary Study

The objectives of the present study were to determine the combined effects of chitosan and water activity (aW) on growth and mycotoxin production in situ on the two most important Fusarium species (F. proliferatum and F. verticillioides) present on maize, and on F. graminearum, the main pathogen causing Fusarium head blight on wheat. Results showed that low-molecular-weight chitosan with more than 70% deacetylation at the lowest dose used (0.5 mg/g) was able to reduce deoxynivalenol (DON) and fumonisin (FBs) production on irradiated maize and wheat grains. Growth rates of F. graminearum also decreased at the lowest chitosan dose used (0.5 mg/g), while F. verticillioides and F. proliferatum growth rates were reduced at 0.98 aW at the highest chitosan dose used (2 mg/g). Since mycotoxins are unavoidable contaminants in food and feed chains, their presence needs to be reduced in order to minimize their effects on human and animal health and to diminish the annual market loss through rejected maize and wheat; in this scenario, pre- and post-harvest use of chitosan could be an important alternative.

Zearalenone and its metabolites: occurrence, detection, toxicity and guidelines

2008 ◽  
Vol 1 (2) ◽  
pp. 209-220 ◽  
Author(s):  
K. Gromadzka ◽  
A. Waskiewicz ◽  
J. Chelkowski ◽  
P. Golinski
Keyword(s):  
Analytical Methods ◽  
Fusarium Species ◽  
Animal Health ◽  
Global Perspective ◽  
Fusarium Mycotoxins ◽  
Food And Feed ◽  
Comparison Of Analytical Methods ◽  
Identification And Quantification

Zearalenone is a mycotoxin produced by some Fusarium species in food and feed. From a global perspective, Fusarium mycotoxins may be considered as metabolites of particular importance to animal health and productivity. The aim of this review is to collect and summarise information concerning the properties of zearalenone, its derivatives and their biotransformation. Data on the occurrence and toxicity of zearalenone and a comparison of analytical methods used in zearalenone identification and quantification will also be discussed. As our awareness and understanding of the risks associated with zearalenone exposure increase, some countries set official or recommended limits in certain commodities.

scholarly journals Microcantilever Resonators for Ochratoxin A Detection in Food and Drinks

2017 ◽  
Author(s):  
Karin Santoro ◽  
Ambra Prelle ◽  
Davide Spadaro ◽  
Maria Lodovica Gullino ◽  
Carlo Ricciardi
Keyword(s):  
Ochratoxin A ◽  
Limit Of Detection ◽  
Animal Health ◽  
Food Contamination ◽  
Grape Juice ◽  
Calibration Plot ◽  
Highly Sensitive ◽  
Wide Range ◽  
Time In Literature ◽  
First Time

Mycotoxins food contamination represents a serious risk for consumers health. They are secondary metabolites of fungi that can be present in a wide range of foodstuffs. Ochratoxin A (OTA) is one of the most toxic compound and it is classified as a possible carcinogenic molecule. The harmful effects of OTA on human and animal health lead to a big boost to develop and optimize highly sensitive and accurate methods for OTA detection. An innovative and rapid detection method based on microcantilever resonators for ochratoxin A identification in food matrix has been developed. This work demonstrates the possibility to apply microcantilever technology in food safety field, showing for the first time in literature the successful detection of one of the most dangerous mycotoxin in different food matrixes both solids and liquids, such as green coffee, grape juice and wine. Sensing performances are discussed in terms of calibration plot and limit of detection.

scholarly journals Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii

2010 ◽  
Vol 27 (Special Issue 2) ◽  
pp. 66-74 ◽  
Author(s):  
I. Hochel ◽  
J. Škvor
Keyword(s):  
Enzyme Immunoassay ◽  
Type Strain ◽  
Polyclonal Antibodies ◽  
Detection Limits ◽  
Enterobacter Sakazakii ◽  
Heat Stable ◽  
Heat Labile ◽  
Heat Stable Antigen ◽  
Immunochemical Detection ◽  
Specific Igg

An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739<sup>T</sup> were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6&ndash;14.4 &times; 10<sup>5</sup> cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.

Microsponges: An Overview

2017 ◽  
Vol 4 (4) ◽  
Author(s):  
Hamid Hussain ◽  
Divya Juyal ◽  
Archana Dhyani
Keyword(s):  
Controlled Release ◽  
Delivery System ◽  
Oral Delivery ◽  
Active Agent ◽  
Topical Delivery ◽  
Water Soluble ◽  
Wide Range ◽  
Porous Beads

Microsponge and Nanosponge delivery System was originally developed for topical delivery of drugs can also be used for controlled oral delivery of drugs using water soluble and bioerodible polymers. Microsponge delivery system (MDS) can entrap wide range of drugs and then release them onto the skin over a time by difussion mechanism to the skin. It is a unique technology for the controlled release of topical agents and consists of nano or micro porous beads loaded with active agent and also use for oral delivery of drugs using bioerodible polymers.

The content of decenoic acids in drone brood preparations and combined preparations based on it

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 389-393
Author(s):  
D.V. Mitrofanov ◽  
N.V. Budnikova
Keyword(s):  
Royal Jelly ◽  
Storage Conditions ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Strict Adherence ◽  
Drone Brood ◽  
Wide Range ◽  
Active Substances ◽  
Melanin Complex

The drone brood contains a large number of substances with antioxidant activity. These substances require stabilization and strict adherence to storage conditions. Among these substances are unique decenoic acids, the content of which is an indicator of the quality of drone brood and products based on it. The ability of drone brood to reduce the manifestations of oxidative stress is shown. There are dietary supplements for food and drugs based on drone brood, which are used for a wide range of diseases. Together with drone brood, chitosan-containing products, propolis, royal jelly can be used. They enrich the composition with their own biologically active substances and affect the preservation of the biologically active substances of the drone brood. Promising are the products containing, in addition to the drone brood, a chitin-chitosan-melanin complex from bees, propolis, royal jelly. The chitin-chitosan-melanin complex in the amount of 5% in the composition of the adsorbent practically does not affect the preservation of decenic acids, while in the amount of 2% and 10% it somewhat worsens. The acid-soluble and water-soluble chitosan of marine crustaceans significantly worsens the preservation of decenoic acids in the product. Drone brood with royal jelly demonstrates a rather high content of decenoic acids. When propolis is introduced into the composition of the product, the content of decenoic acids increases according to the content of propolis.

scholarly journals Probe-Based Real-Time qPCR Assays for a Reliable Differentiation of Capripox Virus Species

2021 ◽  
Vol 9 (4) ◽  
pp. 765
Author(s):  
Janika Wolff ◽  
Martin Beer ◽  
Bernd Hoffmann
Keyword(s):  
Real Time ◽  
Animal Health ◽  
Diagnostic Methods ◽  
Virus Species ◽  
Time Saving ◽  
Robust Detection ◽  
Highly Sensitive ◽  
Reliable Differentiation ◽  
World Organization ◽  
Real Time Qpcr

Outbreaks of the three capripox virus species, namely lumpy skin disease virus, sheeppox virus, and goatpox virus, severely affect animal health and both national and international economies. Therefore, the World Organization for Animal Health (OIE) classified them as notifiable diseases. Until now, discrimination of capripox virus species was possible by using different conventional PCR protocols. However, more sophisticated probe-based real-time qPCR systems addressing this issue are, to our knowledge, still missing. In the present study, we developed several duplex qPCR assays consisting of different types of fluorescence-labelled probes that are highly sensitive and show a high analytical specificity. Finally, our assays were combined with already published diagnostic methods to a diagnostic workflow that enables time-saving, reliable, and robust detection, differentiation, and characterization of capripox virus isolates.

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