Phenotypic and Genotypic Characterization of Escherichia coli Verotoxin-Producing Isolates from Humans and Pigs

2001 ◽  
Vol 64 (12) ◽  
pp. 1904-1911 ◽  
Author(s):  
ANNIE DesROSIERS ◽  
JOHN M. FAIRBROTHER ◽  
ROGER P. JOHNSON ◽  
CLARISSE DESAUTELS ◽  
ANN LETELLIER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Hemorrhagic Colitis ◽  
Testing Technique ◽  
Fecal Samples ◽  
The Individual ◽  
Phenotypic And Genotypic Characterization

The aim of this study was to characterize verotoxin-producing Escherichia coli (VTEC) isolates obtained from humans and pigs in the same geographic areas and during the same period of time in order to determine whether porcine VTEC isolates could be related to human cases of diarrhea and also to detect the presence of virulence factors in these isolates. From 1,352 human and 620 porcine fecal samples, 11 human and 18 porcine verotoxin-positive isolates were obtained by the VT immunoblot or the individual colony testing technique. In addition, 52 porcine VTEC strains isolated from diseased pigs at the Faculté de médecine vétérinaire during the same period or from fecal samples collected previously isolated at slaughterhouses were characterized in this study. Antimicrobial resistance profiles were different between human and porcine isolates. In general, the serotypes observed in the two groups were different. No porcine isolate was of serotype O157:H7; however, one isolate was O91:NM, a serotype that has been associated with hemorrhagic colitis in humans. Also, one serotype (O8:H19) was found in isolates from both species; however, the O8:H19 isolates of the two groups were of different pathotypes. The pathotypes observed in the human and porcine isolates were different, with the exception of VT2vx-positive isolates; the serotypes of these isolates from the two groups were nevertheless different. Pulsed-field gel electrophoresis analysis indicated no relatedness between the human and porcine isolates. In conclusion, these results suggest that the porcine and human isolates of the present study were not genetically related. Most porcine VTEC isolates did not possess known virulence factors required to infect humans. However, certain non-O157:H7 porcine VTECs may potentially infect humans.

scholarly journals Longitudinal Study of Shiga Toxin-Producing Escherichia coli Shedding in Sheep Feces: Persistence of Specific Clones in Sheep Flocks

2009 ◽  
Vol 75 (6) ◽  
pp. 1769-1773 ◽  
Author(s):  
Sergio Sánchez ◽  
Remigio Martínez ◽  
Alfredo García ◽  
Jorge Blanco ◽  
Jesús E. Blanco ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Longitudinal Study ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Individual Animal ◽  
Pulsed Field ◽  
The Individual ◽  
Sheep Feces

ABSTRACT To provide information on the persistence and maintenance of colonization with Shiga toxin-producing Escherichia coli (STEC) in sheep, pulsed-field gel electrophoresis analysis of STEC isolates (n = 145) belonging to serogroups O5, O91, and O146 from 39 healthy animals was performed in a 12-month longitudinal study carried out with four sheep flocks. At the flock level as well as the individual-animal level, the same clones were obtained on sampling occasions separated by as much as 11 months.

Characterization of the Risk to Human Health of Pathogenic Escherichia coli Isolates from Chicken Carcasses

1999 ◽  
Vol 62 (7) ◽  
pp. 741-746 ◽  
Author(s):  
FRANÇOIS CAYA ◽  
JOHN M. FAIRBROTHER ◽  
LOUISE LESSARD ◽  
SYLVAIN QUESSY
Keyword(s):  
Escherichia Coli ◽  
Human Health ◽  
Gel Electrophoresis ◽  
Geographical Area ◽  
Pulsed Field Gel Electrophoresis ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Uremic Syndrome ◽  
Tract Infections

The purpose of this study was to evaluate the risk for human health associated with pathogenic Escherichia coli isolated from airsacculitis and cellulitis in chickens, by comparing the genotypic and phenotypic profiles of avian E. coli isolates and E. coli strains isolated from sick humans during the same period and in the same geographical area as the avian isolates. A total of 96 isolates and 46 isolates from lesions of cellulitis and airsacculitis, respectively, were obtained. Isolates from the backs of some of the affected and healthy birds and 91 intestinal and extraintestinal isolates from humans with diarrhea, urinary tract infections, or septicemia were examined. The frequency of antimicrobial resistance was in general higher in the avian than in the human isolates. VT1-VT2-Eae and VT2-Eae, pathotypes associated with hemolytic and uremic syndrome and bloody diarrhea in humans, were the most frequently encountered pathotypes in human intestinal isolates but were not recovered from the avian isolates. Aero-Pap-TSH and Aero-TSH were the most frequently encountered pathotypes in avian isolates but were rarely observed in human isolates. No avian isolate was of serogroup O157, whereas many human isolates belonged to this O group. O78 and O2 were the most frequently observed O groups in avian isolates but were rarely found in human isolates. Only two avian isolates demonstrated possible relatedness to human isolates based on pulsed-field gel electrophoresis profiles, but they belonged to different pathotypes. Our results suggest that avian isolates recovered from cellulitis and air sacullitis possess very few of the attributes required to cause diseases in humans. It is also concluded that isolates from cellulitis and airsacculitis do not represent a greater hazard than isolates from the back of healthy birds.

Prevalence and Characterization of Escherichia coli O157:H7 on Pork Carcasses and in Swine Colon Contents from Provincially Licensed Abattoirs in Alberta, Canada

2020 ◽  
Vol 83 (11) ◽  
pp. 1909-1917
Author(s):  
SAIDA ESSENDOUBI ◽  
XIANQIN YANG ◽  
ROBIN KING ◽  
JULIA KEENLISIDE ◽  
JAVIER BAHAMON ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Mean Value ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Pulsed Field ◽  
E Coli ◽  
Swine Farms

ABSTRACT The objective of this study was to determine the prevalence of Shiga toxin–producing Escherichia coli (STEC) O157:H7 in colon contents and on carcasses from pigs slaughtered at provincially licensed abattoirs (PLAs) in Alberta, Canada. In 2017, carcass sponge samples and colon content samples were collected from 504 healthy market hogs at 39 PLAs and analyzed for E. coli O157:H7. Carcass samples were also analyzed for E. coli and aerobic colony count (ACC). Nine (1.8%) of 504 carcass samples were confirmed positive for E. coli O157:H7. Seven (1.4%) of 504 colon content samples were confirmed positive for E. coli O157:H7. These positives were found in 5 (12.8%) of 39 PLAs from hogs originating from eight farms. The E. coli O157:H7 isolates recovered from the positive samples (n = 1 isolate per sample) were clonal, as determined by pulsed-field gel electrophoresis. Six E. coli O157:H7 isolates obtained over 8 months from one PLA that only processed hogs and sourced hogs from one farm had indistinguishable pulsed-field gel electrophoresis patterns. All 16 E. coli O157:H7 isolates harbored eae and ehxA and were of stx2a subtype, suggesting that swine can carry E. coli O157:H7 of importance to human health. All carcass sponge swabs (100%) were positive for ACC. E. coli was present in 72% of carcass swabs. Carcasses from PLAs slaughtering both beef and hogs had a numerically higher ACC mean value but not statistically different compared with the carcasses from PLAs slaughtering only swine (2,799 and 610 CFU/cm2, respectively). E. coli showed a similar trend with a mean value of 0.88 CFU/cm2 in PLAs slaughtering both species and 0.26 CFU/cm2 in PLAs slaughtering only swine (P ≤ 0.05). This study provides evidence that healthy market hogs from different producers and farms in Alberta can carry E. coli O157:H7, and some strains of the organism may be able to establish persistence on some swine farms. HIGHLIGHTS

scholarly journals Monitoring and Characterization of Extended-Spectrum β-Lactamases in Escherichia coli Strains from Healthy and Sick Animals in Spain in 2003

2005 ◽  
Vol 49 (3) ◽  
pp. 1262-1264 ◽  
Author(s):  
Laura Briñas ◽  
Miguel Angel Moreno ◽  
Tirushet Teshager ◽  
Yolanda Sáenz ◽  
María Concepción Porrero ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Extended Spectrum ◽  
Genes Encoding ◽  
M 14

ABSTRACT Genes encoding CTX-M-14, CTX-M-9, CTX-M-1, CTX-M-32, SHV-12, TEM-52, or CMY-2 β-lactamases were detected in 21 Escherichia coli strains recovered during 2003 from sick animals (11 of 459 [2.4%] strains) and healthy animals (10 of 158 [6.3%] strains) in Spain. Twelve of these strains harbored bla CTX-M genes and showed unrelated pulsed-field gel electrophoresis patterns.

scholarly journals Comparison of Rectoanal Mucosal Swab Cultures and Fecal Cultures for Determining Prevalence of Escherichia coli O157:H7 in Feedlot Cattle

2005 ◽  
Vol 71 (10) ◽  
pp. 6431-6433 ◽  
Author(s):  
M. A. Greenquist ◽  
J. S. Drouillard ◽  
J. M. Sargeant ◽  
B. E. Depenbusch ◽  
Xiaorong Shi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Feedlot Cattle ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Fecal Culture ◽  
Fecal Samples ◽  
Pulsed Field ◽  
E Coli ◽  
Fecal Sampling

ABSTRACT We compared fecal samples with samples collected with rectoanal mucosa swabs (RAMS) to determine the prevalence of Escherichia coli O157 in feedlot cattle (n = 747). Escherichia coli O157 was detected in 9.5% of samples collected with RAMS and 4.7% of samples tested by fecal culture. Pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal junction were the same as those from the feces. Mucosal swab sampling was more sensitive than fecal sampling for determining the prevalence of E. coli O157 in feedlot cattle.

scholarly journals Carriage of Escherichia coli Producing CTX-M-Type Extended-Spectrum β-Lactamase in Healthy Vietnamese Individuals

2015 ◽  
Vol 59 (10) ◽  
pp. 6611-6614 ◽  
Author(s):  
Thi Mai Huong Bui ◽  
Itaru Hirai ◽  
Shuhei Ueda ◽  
Thi Kim Ngan Bui ◽  
Kouta Hamamoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Fecal Samples ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTHealthy carriage of CTX-M-type extended-spectrum β-lactamase (ESBL)-producingEscherichia coliwas examined by thrice collecting fecal samples from the same 199 healthy Vietnamese subjects every 6 months. Using pulsed-field gel electrophoresis (PFGE), identical PFGE patterns throughout the three samplings were not observed, although prevalence ofE. coliin the subjects was around 50% in the three samplings. Our results suggested a short carriage period of the CTX-M-type ESBL-producingE. coliin healthy Vietnamese subjects.

scholarly journals Characterization of Shiga Toxin-Producing Escherichia coli Isolates Associated with Two Multistate Food-Borne Outbreaks That Occurred in 2006

2007 ◽  
Vol 74 (4) ◽  
pp. 1268-1272 ◽  
Author(s):  
G. A. Uhlich ◽  
J. R. Sinclair ◽  
N. G. Warren ◽  
W. A. Chmielecki ◽  
P. Fratamico
Keyword(s):  
Escherichia Coli ◽  
Stress Responses ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Biofilm Formation ◽  
Pulsed Field Gel Electrophoresis ◽  
E Coli ◽  
Food Borne ◽  
Variant Genes

ABSTRACT Shiga toxin-producing Escherichia coli isolates from two 2006 outbreaks were compared to other O157:H7 isolates for virulence genotype, biofilm formation, and stress responses. Spinach- and lettuce-related-outbreak strains had similar pulsed-field gel electrophoresis patterns, and all carried both stx 2 and stx 2c variant genes. Cooperative biofilm formation involving an E. coli O157:H7 strain and a non-O157:H7 strain was also demonstrated.

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