Control of Penicillium expansum and Botrytis cinerea on Apples and Pears with the Combination of Candida sake and Pantoea agglomerans

2002 ◽  
Vol 65 (1) ◽  
pp. 178-184 ◽  
Author(s):  
CARLA NUNES ◽  
JOSEP USALL ◽  
NEUS TEIXIDÓ ◽  
ROSARIO TORRES ◽  
IMMACULADA VIÑAS
Keyword(s):  
Botrytis Cinerea ◽  
Room Temperature ◽  
Population Level ◽  
Lesion Size ◽  
Gray Mold ◽  
Pantoea Agglomerans ◽  
Penicillium Expansum ◽  
Blue Mold ◽  
Candida Sake ◽  
Stable Community

The effectiveness of Candida sake (CPA-1) in combination with Pantoea agglomerans (CPA-2) for controlling Penicillium expansum and Botrytis cinerea on pears and apples was determined. The concentrations tested were 2 × 106 and 2 × 107 CFU/ml for C. sake and 2 × 107 and 8 × 107 CFU/ml for P. agglomerans. At room temperature, the two antagonists were combined in proportions of 0 to 100% in 25% increments. At the proportion of 50:50, no rot development was observed in pears, and the greatest control of blue mold in apples was observed at this proportion for all the tested concentrations. Under cold temperature on pears, the highest effectiveness of the mixture was observed when C. sake at 2 × 107 CFU/ml was combined with P. agglomerans at 2 × 107 or at 8 × 107 CFU/ml at the proportion 50:50. Under these conditions, no rot development of blue mold was reported, and gray mold lesion size was reduced by more than 95%. On apples, the mixture of C. sake at 2 × 107 CFU/ml and P. agglomerans at 8 × 107 CFU/ml at the proportion 50:50 reduced blue and gray mold incidence by 90%. Populations of the two antagonists had the same growth pattern at 20°C when they were applied individually or in combination, but the population level was always higher when they grew alone. In contrast, at 1°C, the population of both antagonists in combination formed a stable community with the same levels as individual application during the first 30 days; after that, C. sake dominated, and P. agglomerans decreased on apples and pears. At both temperatures, the maximum population level of C. sake was observed in apples, and the maximum population level of P. agglomerans was observed in pears.

scholarly journals Improved Control of Postharvest Decay of Pears by the Combination of Candida sake (CPA-1) and Ammonium Molybdate

2002 ◽  
Vol 92 (3) ◽  
pp. 281-287 ◽  
Author(s):  
Carla Nunes ◽  
Josep Usall ◽  
Neus Teixidó ◽  
Maribel Abadias ◽  
Immaculada Viñas
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Ammonium Molybdate ◽  
Penicillium Expansum ◽  
Low Oxygen ◽  
Blue Mold ◽  
Postharvest Decay ◽  
Oxygen Conditions ◽  
Candida Sake

The potential enhancement of Candida sake (CPA-1) by ammonium molybdate to control blue and gray mold caused by Penicillium expansum and Botrytis cinerea, respectively, on Blanquilla pears was investigated. In laboratory trials, improved control of blue and gray molds was obtained with the application of ammonium molybdate (1, 5, 10, and 15 mM) alone or in combination with C. sake at 2 × 106 or 2 × 107 CFU ml-1 on Blanquilla pears stored at 20°C. In semicommercial trials at 1°C for 5 months, the efficacy of C. sake at 2 × 106 CFU ml-1 on reducing P. expansum and B. cinerea decay was enhanced more than 88% with the addition of ammonium molybdate 5 mM in the 1999-2000 season. In two seasons, the performance C. sake at 2 × 106 CFU ml-1 plus ammonium molybdate was similar to or greater than that of C. sake at 2 × 107 CFU ml-1. Similar control of blue mold was obtained on pears stored under low oxygen conditions. The preharvest application of ammonium molybdate did not reduce postharvest blue mold decay. The population of C. sake on pear wounds significantly decreased in the presence of ammonium molybdate 1 and 5 mM at 20 and 1°C.

scholarly journals Control of Blue Mold of Apples by Preharvest Application of Candida sake Grown in Media with Different Water Activity

1998 ◽  
Vol 88 (9) ◽  
pp. 960-964 ◽  
Author(s):  
N. Teixidó ◽  
I. Viñas ◽  
J. Usall ◽  
N. Magan
Keyword(s):  
Population Size ◽  
Water Activity ◽  
Cold Storage ◽  
Storage Conditions ◽  
Lesion Size ◽  
Penicillium Expansum ◽  
Laboratory Studies ◽  
Blue Mold ◽  
Infected Wounds ◽  
Candida Sake

Unmodified and low water activity (aw)-tolerant cells of Candida sake CPA-1 applied before harvest were compared for ability to control blue mold of apples (‘Golden Delicious’) caused by Penicillium expansum under commercial storage conditions. The population dynamics of strain CPA-1 on apples were studied in the orchard and during storage following application of 3 × 106 CFU/ml of each treatment 2 days prior to harvest. In the field, the population size of the unmodified treatment remained relatively unchanged, while the population size of the low-aw-modified CPA-1 cells increased. During cold storage, the populations in both treatments increased from 103 to 105 CFU/g of apple after 30 days, and then declined to about 2.5 × 104 CFU/g of apple. In laboratory studies, the low-aw-tolerant cells provided significantly better disease control as compared with the unmodified cells and reduced the number of infected wounds and lesion size by 75 and 90%, respectively, as compared with the non-treated controls. After 4 months in cold storage, both unmodified and low-aw-tolerant cells of C. sake were equally effective against P. expansum on apple (>50% reduction in size of infected wounds).

scholarly journals Disease Incidence—Inoculum Dose Relationships for Botrytis cinerea and Penicillium expansum and Decay of Pear Fruit Using Dry, Airborne Conidia

Plant Disease ◽  
2001 ◽  
Vol 85 (7) ◽  
pp. 755-759 ◽  
Author(s):  
Robert A. Spotts ◽  
Louis A. Cervantes
Keyword(s):  
Botrytis Cinerea ◽  
Disease Incidence ◽  
Gray Mold ◽  
Fruit Rot ◽  
Penicillium Expansum ◽  
Pear Fruit ◽  
Blue Mold ◽  
Inoculum Dose ◽  
Airborne Concentration ◽  
Fruit Decay

The objective of this research was to determine quantitative relationships between incidence of pear fruit decay and inoculum dose of Botrytis cinerea and Penicillium expansum using dry conidia applied to wet or dry pears in a settling tower. On wet fruit, incidence of gray mold fruit rot increased from 0.1 to 83.1% as the airborne concentration of B. cinerea conidia increased from 0 to 8.6 spores per liter of air. Significantly less decay occurred in fruit inoculated dry compared to wet, particularly in fruit wounded after inoculation. Incidence of blue mold increased from 1 to 100% as the airborne concentration of P. expansum conidia increased from 0.1 to 803.5 spores per liter of air. Blue mold incidence was not affected by fruit wetness or time of wounding relative to inoculation. All regressions of decay incidence versus airborne and surface conidial concentrations were highly significant (P = 0.01).

scholarly journals Methodology for Determining Relationships Between Inoculum Concentration of Botrytis cinerea and Penicillium expansum and Stem End Decay of Pear Fruit

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 451-455 ◽  
Author(s):  
Robert A. Spotts ◽  
Kelly M. Wallis ◽  
Maryna Serdani ◽  
Daniel T. O'Gorman ◽  
Peter L. Sholberg
Keyword(s):  
Linear Regression ◽  
Real Time ◽  
Botrytis Cinerea ◽  
Gray Mold ◽  
Penicillium Expansum ◽  
Pear Fruit ◽  
Blue Mold ◽  
Inoculum Concentration ◽  
Inoculum Dose ◽  
Fruit Surface

The objective of this research was to determine quantitative relationships between incidence of stem end decay of pear fruit and inoculum concentration of Botrytis cinerea and Penicillium expansum using dry conidia applied to pear fruit in a settling tower. Five concentrations of conidia were applied to pear fruit, fruit were stored at –1°C for 8 months, and stem end decay was evaluated. In addition, conidia were washed from the surface of inoculated fruit, and DNA was extracted and quantified with real-time polymerase chain reaction (PCR). The linear regression relationships between percent stem end gray mold and B. cinerea conidia per liter of air or per square centimeter of fruit surface were significant (P = 0.01). At the highest inoculum dose introduced into the settling tower, conidia per liter of air, conidia per square centimeter, and percent stem end gray mold at 8 months after inoculation were 12, 31, and 39, respectively for 2000 and 6, 33, and 67, respectively for 2001. Similarly, the linear regression relationships between percent stem end blue mold and P. expansum conidia per liter of air or per square centimeter of fruit surface were significant (P = 0.01 and 0.05, respectively). At the highest inoculum dose introduced into the settling tower, conidia per square centimeter and percent stem end blue mold at 8 months after inoculation were 39 and 26, respectively for 2000 and 66 and 23, respectively for 2003. Real-time PCR provided a rapid, quantitative measure of B. cinerea and P. expansum DNA on pear fruit surfaces. Because of possible year-to-year shifts in susceptibility of fruit to decay, disease incidence:inoculum dose relationships may be of most value compared within years rather than across years. This would facilitate comparison of decay risk among orchards in order to determine which fruit is most suitable for long-term storage.

scholarly journals Preharvest Application of a Boscalid and Pyraclostrobin Mixture to Control Postharvest Gray Mold and Blue Mold in Apples

Plant Disease ◽  
2009 ◽  
Vol 93 (2) ◽  
pp. 185-189 ◽  
Author(s):  
C. L. Xiao ◽  
R. J. Boal
Keyword(s):  
Botrytis Cinerea ◽  
Cold Storage ◽  
Malus Domestica ◽  
Gray Mold ◽  
Alternative Strategy ◽  
Fruit Rot ◽  
Effective Alternative ◽  
Penicillium Expansum ◽  
Blue Mold

After harvest, apples (Malus × domestica) may be kept in cold storage for up to 12 months prior to packing. Gray mold caused by Botrytis cinerea and blue mold caused by Penicillium expansum are common postharvest fruit rot diseases affecting apples and are controlled commonly by applications of fungicides after harvest. To search for an alternative strategy, Pristine (a premixed formulation of boscalid and pyraclostrobin) as a preharvest treatment was evaluated for control of postharvest gray mold and blue mold in cultivars Fuji and Red Delicious apples during 2004 to 2006. Pristine (0.36 g per liter of water) was applied 1, 7, or 14 days before harvest. For comparison, thiram (2.04 g per liter of water) was applied 7 days before harvest and ziram (2.4 g per liter of water) was applied 14 days before harvest, to Fuji and Red Delicious, respectively. Fruit were harvested at commercial maturity, wounded with a finishing nail head, inoculated with conidial suspensions of either B. cinerea or P. expansum, stored in air at 0°C, and evaluated for decay after 8 or 12 weeks. In 2004 and 2005, Pristine was equally effective when applied to Fuji 1 or 7 days before harvest, reducing gray mold incidence by 93 to 99% and blue mold incidence by 78 to 94% compared with the nontreated control. Thiram reduced gray mold incidence by 38 to 85%. Thiram reduced blue mold incidence by 22% in 2004 but not in 2005. On Red Delicious, Pristine was equally effective when applied 7 or 14 days before harvest and reduced gray mold incidence by 69 to 85% and blue mold incidence by 41 to 70%. Ziram applied 2 weeks before harvest reduced gray mold incidence by 97 and 94% in 2005 and 2006, respectively, but it did not reduce blue mold incidence. The results indicate that Pristine applied within 2 weeks before harvest may be an effective alternative to postharvest fungicides for control of postharvest gray mold and blue mold in Fuji and Red Delicious apples.

scholarly journals Fumigation of Stored Pome Fruit with Hexanal Reduces Blue and Gray Mold Decay

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 611-616 ◽  
Author(s):  
Peter L. Sholberg ◽  
Paul Randall
Keyword(s):  
Titratable Acidity ◽  
Polynomial Equation ◽  
Gray Mold ◽  
Soluble Solids ◽  
Penicillium Expansum ◽  
Effective Control ◽  
Blue Mold ◽  
Pome Fruit ◽  
Third Order ◽  
Apple Slices

Stored apples and pears are subject to blue and gray mold decay incited by Penicillium expansum and Botrytis cinerea respectively. Hexanal, a C6 carbon aldehyde, used as a vapor provided effective control of both blue and gray molds in laboratory experiments on apple slices. A preliminary trial with ‘Anjou’ pears in bins showed that hexanal was not corrosive and could reduce gray mold in pears stored for 7 months. However details on the correct procedure for fumigating pome fruit were lacking, and further studies were needed to develop a reliable fumigation strategy. In trials with inoculated fruit, hexanal inactivated conidia of B. cinerea contaminating the pear surface when used at a rate of 2 mg·L−1 for 24 hours or 4 mg·L−1 for 18 hours. It was less effective on ‘Gala’ apples inoculated with conidia of P. expansum, but reduced blue mold decay to low levels at 15 ºC. On the other hand, hexanal increased gray and blue molds when used after wounds were made in inoculated fruit. The use of a preharvest treatment with cyprodinil (0.62 g·L−1) reduced both blue and gray molds in wounds with or without hexanal fumigation. Thus a strategy for controlling postharvest decay was developed by which fruit were treated 2 weeks before harvest with cyprodinil, followed by fumigation with hexanal immediately after harvest. The use of this strategy on ‘Anjou’ pears produced the highest number of mold-free fruit in 2003 and the least amount of gray and blue mold decay in 2003 and 2004 on pears stored for 4 months. Wounded apples only developed 1% rot compared with 10% in the control, indicating that hexanal fumigation of stored apples reduced contamination. Monitoring hexanal during fumigation showed that hexanal concentration declined slowly over a 24-hour period and could accurately be described by a third-order polynomial equation. Hexanal fumigation at low rates (2–3 mg·L−1) was not phytotoxic and improved aroma in ‘Anjou’ pears and ‘Gala’ apples with no harmful effects on apple or pear firmness, pH, titratable acidity, or soluble solids.

scholarly journals The occurrence of postharvest diseases on apples resistant to scab

2012 ◽  
Vol 58 (2) ◽  
pp. 205-212 ◽  
Author(s):  
Hanna Bryk ◽  
Dorota Kruczyńska
Keyword(s):  
Brown Rot ◽  
Gray Mold ◽  
Penicillium Expansum ◽  
Postharvest Diseases ◽  
Blue Mold ◽  
Monilinia Fructigena ◽  
Storage Diseases ◽  
Bitter Pit ◽  
Apple Cultivars ◽  
Bull's Eye

The occurrence of storage diseases on fruit of seven scab resistant apple cultivars (Freedom, Rajka, Topaz, Rubinola, Enterprise, Goldstar, GoldRush) grafted on M.9 was investigated in 2001-2005. The trees were planted in 1995. It was found that after storage (4 and 6 months at 2<sup>°</sup>C, 85-90% RH) the most severe appeared to be bull's eye rot (<i>Pezicula</i> spp.). The most sensitive cultivars to this disease were: Topaz, Freedom, Goldstar, the least sensitive were Rubinola, Enterprise, Rajka. Other postharvest diseases like gray mold (<i>Botrytis cinerea</i>), blue mold (<i>Penicillium expansum</i>) and brown rot (<i>Monilinia fructigena</i>) were not common. 'Rajka' and 'Goldstar' were susceptible to bitter pit, and 'Freedom' to superficial scald.

scholarly journals Biocontrol Efficacy of Antagonist Yeasts to Gray Mold and Blue Mold on Apples and Pears in Controlled Atmospheres

Plant Disease ◽  
2002 ◽  
Vol 86 (8) ◽  
pp. 848-853 ◽  
Author(s):  
Shiping Tian ◽  
Qing Fan ◽  
Yong Xu ◽  
Haibo Liu
Keyword(s):  
Pathogenic Fungi ◽  
Storage Conditions ◽  
Gray Mold ◽  
Penicillium Expansum ◽  
Cryptococcus Albidus ◽  
Blue Mold ◽  
Controlled Atmospheres ◽  
Biocontrol Efficacy ◽  
Significant Difference ◽  
Apple Fruits

Biocontrol capability of the yeasts Trichosporon sp. and Cryptococcus albidus against Botrytis cinerea and Penicillium expansum was evaluated in apple (cv. Golden Delicious) and pear (cv. Jingbai) fruits at 1°C in air and under controlled atmospheres (CA) with 3% O2 + 3% CO2 or 3% O2 + 8% CO2. Trichosporon sp. controlled gray mold and blue mold of apple fruits more effectively than C. albidus (P < 0.05). Apple fruits treated with Trichosporon sp. and C. albidus had a lower incidence of gray mold rot than blue mold rot in the same storage conditions. Biocontrol efficacy of the yeasts for controlling gray mold and blue mold was better in apples than in pears. Populations of the yeasts in drop-inoculated wounds in fruits increased rapidly after 20 days at 1°C both in air and in CA conditions. There was no significant difference in colony diameters of the two pathogens cultured in 0 to 15% CO2 concentrations after 7 days at 20°C, but the colony diameter of both B. cinerea and P. expansum at 20% CO2 was significantly less than in other treatments (P < 0.05). CA with 3% O2 + 8% CO2 inhibited the pathogenic fungi more than CA with 3% O2 + 3% CO2.

scholarly journals Improved Control of Apple and Citrus Fruit Decay with a Combination of Candida saitoana and 2-Deoxy-D-Glucose

Plant Disease ◽  
2000 ◽  
Vol 84 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Ahmed El-Ghaouth ◽  
Joseph L. Smilanick ◽  
Michael Wisniewski ◽  
Charles L. Wilson
Keyword(s):  
Botrytis Cinerea ◽  
Citrus Fruit ◽  
Penicillium Expansum ◽  
Disease Development ◽  
Blue Mold ◽  
Green Mold ◽  
Fruit Decay ◽  
Orange Fruit ◽  
Water Treated Control

A combination of Candida saitoana with 0.2% 2-deoxy-D-glucose to control decay of apple, lemon, and orange fruit was evaluated. Growth of C. saitoana in vitro was reduced by 2-deoxy-D-glucose; however, in apple wounds, the yeast grew as well in the presence of 2-deoxy-D-glucose as in its absence. When applied to fruit wounds before inoculation, the combination of C. saitoana with 0.2% 2-deoxy-D-glucose was more effective in controlling decay of apple, orange, and lemon caused by Botrytis cinerea, Penicillium expansum, and P. digitatum than either C. saitoana or the application of a 0.2% solution of 2-deoxy-D-glucose alone. Increasing the concentration of 2-deoxy-D-glucose from 0.2 to 0.5% did not improve control significantly. The combination of C. saitoana with 0.2% 2-deoxy-D-glucose was also effective against infections established up to 24 h before treatment. When applied within 24 h after inoculation, the combination of C. saitoana with 0.2% 2-deoxy-D-glucose was very effective in controlling blue mold of apple and green mold of orange and lemon. The level of control of green mold was equivalent to imazalil treatment. When either C. saitoana or 0.2% 2-deoxy-D-glucose was applied within 24 h after inoculation, neither had an effect on disease development on apple, orange, or lemon, and the incidence of decay was similar to the water-treated control.

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