scholarly journals Granulation tissue-derived mesenchymal stromal cells: a potential application for burn wound healing in pediatric patients

2018 ◽  
Vol 14 (1) ◽  
pp. 53-58 ◽  
Keyword(s):  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Granulation Tissue ◽  
Stromal Cells ◽  
Potential Application ◽  
Pediatric Patients ◽  
Burn Wound ◽  
Burn Wound Healing

scholarly journals Pengaruh Papain Getah Pepaya Terhadap Pembentukan Jaringan Granulasi pada Penyembuhan Luka Bakar Tikus Percobaan

2014 ◽  
Vol 3 (1) ◽  
Author(s):  
Meishinta Fitria ◽  
Deddy Saputra ◽  
Gusti Revilla
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Control Group ◽  
Tissue Formation ◽  
Burn Wound ◽  
Control Group Design ◽  
Granulation Tissue Formation ◽  
Burn Wounds ◽  
Group Design ◽  
Burn Wound Healing

AbstrakLuka bakar merupakan masalah yang serius dalam kesehatan dunia, khususnya di negara berkembang. Di Indonesia belum ada laporan tertulis mengenai jumlah penderita luka bakar dan jumlah angka kematian yang diakibatkannya. Di RSUP Dr. M. Djamil Padang pada tahun 2010 ditemukan 84 kasus luka bakar dengan penyebab sengatan listrik, siraman air panas, kompor, dan minyak panas. Sejumlah studi menunjukkan bahwa tanaman tradisional potensial sebagai agen penyembuhan luka, salah satunya papain getah pepaya (Carica papaya). Penelitian ini bertujuan untuk mengetahui pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaan. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan posttest only control group design. Subjek penelitian adalah 10 ekor tikus Wistar jantan yang dibagi menjadi dua kelompok, yaitu kelompok kontrol (K) dan kelompok perlakuan (P). Masing-masing kelompok terdiri dari lima (5) ekor tikus. Plat logam (1,5 cm x 1,5 cm) yang dipanaskan digunakan untuk menghasilkan luka bakar full thickness pada bagian dorsal tikus. Papain getah pepaya diberikan pada kelompok P selama 7 hari. Hasil penelitian ini menunjukkan bahwa pemberian papain getah pepaya tidak memberikan pengaruh yang signifikan (p > 0,05) terhadap pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaan. Pada kelompok P didapatkan hasil pembuluh darah 29,26 ± 12,34, fibroblas 26,40 ± 21,94, neutrofil 1,4 ± 0,44, limfosit 1,06 ± 0,13, dan makrofag 1,00 ± 0,00. Kesimpulan penelitian ini adalah papain getah pepaya tidak memberikan pengaruh yang signifikan terhadap pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaanKata kunci: papain, jaringan granulasi, luka bakar, penyembuhan lukaAbstractBurn wounds is the serious problem in world health specifically for developing contries. In Indonesia, there is no written report about burn wounds patient and mortality account yet. In 2010, M. Djamil Padang Government Public Hospital found 84 cases of burn wounds with some causes as burn effect from sting of electric, hot water, stove flame and hot oil. Some researches indicate that traditional plant able to be wound healing agent as papaya sap. The purposed of this studi was to find out the effect of papain from papaya sap to granulation tissue formation on burn wounds healing in rat models. This was experimental research with posttest only control group design. The subjects were ten male Wistar rats divided in to two group (control group K and experimental group P). Every group consist of five rats. Heated metal plat (1,5 cm x 1,5 cm) used to get full thickness burn wound on dorsal rat part. Then, papain of papaya sap was given to group P for seven days. The results showed that papain of papaya sap didn’t have significant effect (p > 0,05) to granulation tissue formation in burn wound healing of rat models. In group P, the research found the vascular 29,26 ± 12,24, fibroblast 26,40 ± 21,94, neutrophil 1,4 ± 0,44, lymphocyte 1,06 ± 0,13, and macrophag 1,00 ± 0,00. The conclusion of this research is that papain of papaya sap didn’t have the significant effect to granulation tissue formation in burn wound healing of rat models.Keywords:papain, granulation tissue, burn, wound healing

Adipose-Derived Stem Cell Condition Medium Enhances Expression of Ephrin-B2 Related to Neovascularization in Burn Wound Tissue

2018 ◽  
Vol 24 (8) ◽  
pp. 6168-6172
Author(s):  
Elvin Clara Angmalisang ◽  
Dewi Sukmawati ◽  
Lia Damayanti ◽  
Astheria Eryani ◽  
Jeanne A Pawitan
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
Granulation Tissue ◽  
Skin Wound ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Wound Tissue

The transmembrane ligand ephrin-B2 is molecular marker for arterial endothelial cells and early blood vessel formation. Neovascularization is one of the important event that support wound healing. Adiposederived stem cells conditioned medium (ADSC-CM) is known to contain cytokines and growth factors as regenerative agent. This study aims to investigate the effect of ADSC-CM on ephrin-B2 expression that represent as neovascularization marker in burn wound granulation tissue. Male Sprague dawley rats were randomly divided into control, conditioned medium (CM) and medium complete (MC) groups. Full-thickness burn wounds were created by placing preheated metal plat on dorsal thorax of the prepared rats. The burn wounds were treated once daily according to their group. Skin wound tissues were collected at day 7, 14, and 21 post wounded for histological analysis. The expression of ephrin-B2 was demonstrated by immunohistochemistry staining. Neovascularization evaluation was done by calculating the number of newly formed capillaries that expressed Ephrin-B2 in wound granulation tissues. Expression of Ephrin-B2 in burn wound tissue was prominently increased in CM compared to control and MC groups on day 7, 14 and 21 post wounded. The CM group also showed higher neovascular number in granulation tissue compared to control and MC group on day 7, 14 and 21 (p < 0.05). Our results showed prominent ephrin-B2 expression in burn wound tissue after treated with ADSC-CM. Topical application of ADSC-CM has shown to promote angiogenesis in full-thickness burn wound in part through the increased of ephrin-B2 expression. Therefore ADSC-CM could act as an alternative strategy to promote burn wound healing.

The Safety and Efficacy of Mupirocin Topical Spray for Burn Wound Healing in A Rat Model

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Sritharadol Rutthapol ◽  
Chunhachaichana Charisopon ◽  
Kumlungmak Sukanjana ◽  
Buatong Wilaiporn ◽  
Dechraksa Janwit ◽  
...  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Immunohistochemical Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Burn Wound ◽  
Safety And Efficacy ◽  
Burn Wound Healing ◽  
Tgf Β1

ABSTRACT This study evaluated the effect of mupirocin topical spray on burn wound healing in a rat model. Fifteen male Sprague Dawley rats were used to create full-thickness burns on the rat dorsum using a cylindrical stainless steel rod. The rats were topically treated with normal saline solution (NSS), mupirocin spray, ointment, and solution. The wound size and morphological evaluation were investigated by photographs and clinical criterions for wound healing. The histology was observed by hematoxylin and eosin (HandE) staining assay. The immunohistochemical study was evaluated by detection of transforming growth factor-beta 1 (TGF-β1), and the ratio of matrix metalloproteinase-9 to the tissue inhibitor of matrix metalloproteinase-1 (MMP-9/TIMP-1) was quantified using the enzyme-linked immunosorbent assay (ELISA) assay. A complete healing was observed at 28 days in all treatments. Mupirocin formulations accelerated the wound healing faster than NSS in size. However, the clinical criteria indicated a desirable skin appearance in the mupirocin spray and ointment treated groups. The histological evaluations showed no differences between the treatments while the immunohistochemical study revealed that all treatments reduced the level of TGF-β1 over time, particularly on day 28 in the mupirocin spray and ointment treated groups. The MMP-9/TIMP-1 ratio was significantly lower in the mupirocin spray and ointment treated groups than in the NSS and mupirocin solution groups. This study shows the safety and efficacy in the use of mupirocin topical spray. The topical mupirocin spray is an alternative suitable for development as a human topical anti-infective and wound protection spray.

scholarly journals Validity of thermography for measuring burn wound healing potential

2019 ◽  
Vol 28 (3) ◽  
pp. 347-354 ◽  
Author(s):  
Michelle E. Carrière ◽  
Louise E. M. Haas ◽  
Anouk Pijpe ◽  
Annebeth Meij‐de Vries ◽  
Kim L. M. Gardien ◽  
...  
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Burn Wound Healing

Evaluation of Potential Application of Wharton’s Jelly-Derived Human Mesenchymal Stromal Cells and its Conditioned Media for Dermal Regeneration using Rat Wound Healing Model

2021 ◽  
pp. 1-14
Author(s):  
Caroline Mathen ◽  
Mrunal Ghag Sawant ◽  
Raghubansh Gupta ◽  
Wilfrid Dsouza ◽  
Shilpa G. Krishna
Keyword(s):  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Therapeutic Potential ◽  
Wound Care ◽  
Free Cell ◽  
Conditioned Media ◽  
Human Umbilical Cord ◽  
Human Mscs ◽  
Wound Model

Mesenchymal stromal cells and the derived conditioned media represent an area of tremendous medical interest and, among other clinical applications, are currently being extensively explored for wound healing. The aim of this study was to comparatively evaluate the wound healing potential of xeno-free human umbilical cord-derived mesenchymal stromal cells (MSCs) and the conditioned media (CM) in a full-thickness excision wound model in rats. The evaluation parameters included rate of wound healing, serum cytokine analyses, collagen content, histopathology, and hyperspectral imaging as an independent qualitative and quantitative tool. Both the cell-based and cell-free approaches scored better in lower inflammation, as evidenced in lower IL-10 and stable IL-6 levels, and improved rate of wound healing (<i>p</i> &#x3c; 0.0001). More importantly, no adverse reaction or rejection was observed although human MSCs and CM were used in a xenogeneic model. The presence of hFGF, hHGF, hGCSF, hIL-1Ra, hVEGF, and hIL-6 in the secretome may elucidate the regenerative potential of the xeno-free cell-based and cell-free approaches which have translational value for advanced wound care. The results revealed the therapeutic potential of both the cell-based and cell-free approaches for wound healing.

556 The Use of a Concentrated Surfactant Based Gel to Promote Burn Wound Healing

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S126-S127
Author(s):  
Rebecca Coffey ◽  
Rachel Penny
Keyword(s):  
Wound Healing ◽  
New Product ◽  
Ease Of Use ◽  
Burn Injuries ◽  
Full Thickness ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Care ◽  
Product Trial ◽  
Burn Wound Healing

Abstract Introduction Strategies to remove necrotic tissue from burn wounds include excisional and non-excisional debridement. Alternative treatments could promote burn wound healing while minimizing patient discomfort and the need for surgery. We evaluated the usage of a concentrated surfactant gel (CSG) to promote burn wound healing in those with indeterminate depth and full thickness burn injuries. Methods An IRB approved retrospective study was conducted during a 10-patient new product trial period with enrollment between September and October 2019. Patients included in this study had indeterminate or full thickness burn wounds and were treated with a concentrated surfactant-based gel. Patients with non-burn diagnoses were excluded. Data collected included demographic information, injury descriptors, and additional burn wound characteristics. Results A total of 10 patients were included in this study as part of a new product trial. The subjects were 80% male with an average TBSA of 7.5%. 40% had indeterminate and 60% had full thickness burn wounds. Prior to initiation of the CSG, the burn wounds had been open for an average of 41 days. There were no infections or complications with usage of the CSG. 90% of patients reported less pain than the standard of care topical agents for burns. Average duration of treatment with the CSG until healing was 28 days. After usage of the CSG, no patients required surgery. Conclusions Our findings support the usage of a concentrated surfactant-based gel in patients with burn wounds. Patients reported decreased pain during dressing changes and ease of use compared to the standard topical agent in burn care. It also prevented surgical debridement in those with indeterminate and full thickness burn injuries.

Burn wound healing: James Ellsworth Laing memorial essay for 1991

Burns ◽  
1992 ◽  
Vol 18 (6) ◽  
pp. 466-478 ◽  
Author(s):  
P. Dziewulski
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Burn Wound Healing
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