Study on Anticancer Activity of Boerhaavia Diffusa on Wistar Albino Rats Treated with Diethyl Nitrosamine

Objective: The present study explored the influence of fluoride exposure on gonadotropins and reproductive hormones.Methods: Male Wistar albino rats weighing 100-150 g were administered with 100, 200, and 300 ppm/kg body weight of sodium fluoride daily for 20 and 40 days. The control group was given 1 ml deionized water/kgbw/day for the same period. At the end of the experimental period, half of the animals were sacrificed and blood serum was collected and testis sample was taken concurrently. Remaining half rats were administrated with two doses (250 mg and 500 mg/kg body weight) of leaf extract of Boerhaavia diffusa L. for another 20 days. Serum level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, and intratesticular level of testosterone were evaluated.Results: It was found that fluoride intoxication resulted in significant increase in the level of FSH (p<0.01) and LH (p<0.0001) while significant decrease in the serum (p<0.01) and testis (p<0.0001) level of testosterone. A significant (p<0.0001) positive correlation was found between level of serum fluoride and gonadotropins: FSH (20 days, r=0.708 and 40 days, r=0.795) and LH (20 days, r=0.779 and 40 days, r=0.891). However, there was significant (p<0.0001) negative correlation existed between serum fluoride and testosterone levels in serum (20 days, r=−0.780 and 40 days, r=−0.862) as well as testis (20 days, r=−0.915 and 40 days, r=−0.938).Conclusion: It was found that administration of B. diffusa L. alleviated the adverse effects of fluoride on reproductive endocrine functions.

Download Full-text

Evaluation of in Vivo Diuretic Activity of Methanolic Extracts of Clutia Abyssinica (Euphorbiaceae) Roots in Wistar Albino Rats

The International Annals of Medicine ◽

10.24087/iam.2017.1.8.215 ◽

2017 ◽

Vol 1 (8) ◽

Author(s):

Abebaw Tegegne ◽

Bharat Mishra ◽

Mestayet Geta

Keyword(s):

Albino Rats ◽

Diuretic Activity ◽

Methanolic Extracts ◽

Wistar Albino Rats

Download Full-text

Role of Oxidative Stress, Pro-Inflammatory Cytokines, Leptin and Adiponectin in Organophosphorus-Induced Insulin Resistance in Wistar Albino Rats

Asian journal of biochemical and pharmaceutical research ◽

10.24214/ajbpr/8/2/5866 ◽

2018 ◽

Vol 8 (2) ◽

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Inflammatory Cytokines ◽

Albino Rats ◽

Wistar Albino Rats ◽

Pro Inflammatory Cytokines

Download Full-text

Preclinical evaluation of hypolipidemic effect of hesperidin in high cholesterol diet model in wistar albino rats

MedPulse International Journal of Pharmacology ◽

10.26611/10101012 ◽

2019 ◽

Vol 10 (1) ◽

pp. 05-08

Author(s):

Pradeep Kumar V ◽

◽

Vijai Sundar E ◽

Keyword(s):

Albino Rats ◽

High Cholesterol Diet ◽

Hypolipidemic Effect ◽

Cholesterol Diet ◽

High Cholesterol ◽

Preclinical Evaluation ◽

Wistar Albino Rats ◽

Diet Model

Download Full-text

Hepatoprotective Activity of Calotropis gigantea Root Bark Experimental Liver Damage Induced by D-Galactosamine in Rats

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2008.1.3.12 ◽

1970 ◽

Vol 1 (3) ◽

pp. 281-286

Author(s):

Pradeep Deshmukh ◽

Tanaji Nandgude ◽

Mahendra Singh Rathode ◽

Anil Midha ◽

Nitin Jaiswal

Keyword(s):

Methyl Cellulose ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Root Bark ◽

Significant Activity ◽

Alcoholic Extract ◽

Calotropis Gigantea ◽

Wistar Albino Rats ◽

Experimental Liver ◽

Hepatoprotective Drug

The suspensions of alcoholic extract of root bark of the plant Calotropis gigantea in 0.6% carboxy methyl cellulose (CMC) were evaluated for hepatoprotective activity in Wistar albino rats by inducing hepatic injury with D-galactosamine (400 mg/kg). Alcoholic extract of root bark of the plant Calotropis gigantea at an oral dose of 200 mg/kg and 400 mg/kg exhibited a significant (P<0.001, P<0.01 and P<0.05) protection effect by normalizing the levels of aspartate amino transferase (ASAT/ GOT), alanine amino transferase (ALAT/GPT), alkaline phosphatase (ALP), total bilirubin (TB), lactate dehydrogenase (LDH), which were significantly (P<0.001) increased in rats by treatment with 400 mg/kg i.p. of D-galactosamine. Silymarin (25 mg/kg), a known hepatoprotective drug used for comparison exhibited significant activity (P<0.001).

Download Full-text

Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.19:217-221 ◽

2020 ◽

Vol 19 (2) ◽

pp. 217-221

Author(s):

Maria Jesús Lisbona-González ◽

Candela Reyes-Botella ◽

Esther Muñoz-Soto ◽

Maria Victoria Olmedo-Gaya, ◽

Jorge Moreno-Fernandez ◽

...

Keyword(s):

Fatty Acids ◽

Body Composition ◽

Adipose Tissue ◽

Endocrine Function ◽

Control Group ◽

Albino Rats ◽

Standard Diet ◽

Reduced Body ◽

Esterified Fatty Acids ◽

Wistar Albino Rats

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

Download Full-text

Renal Alterations Induced by Chronic Exposure to Therapeutic Doses of Antihypercholestremic Atorvastatin

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530321666210106105059 ◽

2021 ◽

Vol 21 ◽

Author(s):

Amin Al-Doaiss ◽

Yazun Jarrar ◽

Ali Shati ◽

Mohammad Alfaifi ◽

Mohammed Al-Kahtani ◽

...

Keyword(s):

Albino Rats ◽

Blood Capillary ◽

Renal Cells ◽

Ultrastructural Alterations ◽

Treatment And Prevention ◽

Basement Membrane Thickening ◽

Wistar Albino Rats ◽

Myelin Figure ◽

Therapeutic Doses ◽

Glucose 6 Phosphate Dehydrogenase

Background: Atorvastatin (ATOR) is widely used for the treatment and prevention of hypercholesterolemia and various diseases, such as cardiovascular complication, with little data about the histopathological and ultrastructural renal alterations that might be induced by this drug. Objectives: The present study was undertaken to investigate the potential toxicity of therapeutic doses of atorvastatin on the microanatomy and ultrastructure of renal tissues from Wistar albino rats. Methods: Adult male Wistar albino rats received an oral daily dose of 5 mg/kg body weight for 90 consecutive days. Biopsies from both kidneys of each study rat were taken for histopathological and ultrastructural examination. Results: ATOR-treated rats exhibited glomerular, tubular, and interstitial histological alterations, including degeneration, necrosis, hyaline droplets, edema, cortical hemorrhages, mesangial hypercellularity, and blood capillary dilation and congestion. In addition, ATOR exposure increased the activity of glucose-6-phosphate dehydrogenase and alkaline phosphatase with a concurrent reduction in proteins and neutral mucosubstances content of the glomeruli and renal cells. Moreover, ATOR-treated animals demonstrated glomerular ultrastructural alterations, consisting mainly of capillary tuft dilatation, glomerular basement membrane thickening, and mesangial cell proliferation. The renal cells of the proximal tubules demonstrated damaged mitochondria, degenerative cellular changes, endoplasmic reticulum dilatation, lysosomal and autophagosome activation, nuclear alteration, myelin figure formation, and microvilli disorganization. Conclusion: The findings of the present work may indicate that ATOR can induce renal histological, histochemical, and ultrastructural alterations that may affect kidney and other vital organ function.

Download Full-text