Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

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Hypolipidemic effect of prepared Polyherbal formulations in Wistar albino rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00749 ◽

2021 ◽

pp. 4314-4320

Author(s):

Ranjan Kumar Giri ◽

Sunil Kumar Kanungo ◽

Saroj Kumar Patro ◽

Minaketan Sahoo ◽

Dibya Sundar Panda

Keyword(s):

Elevated Serum ◽

Density Lipoprotein ◽

Lipid Lowering ◽

Control Group ◽

Albino Rats ◽

Histopathological Study ◽

Oral Glucose ◽

Hypolipidemic Effect ◽

Standard Drug ◽

Wistar Albino Rats

Lipid lowering effect of polyherbal formulations using eight different plants was evaluated in triton and diet induced hyperlipidemic models of wistar albino rats. Formulations such as Tablet, Syrup and Suspension inhibited the elevation in serum cholesterol and triglyceride levels on Triton WR 1339 administration rats. The formulations at the same dose level significantly attenuated the elevated serum total cholesterol and triglycerides with an increase in high-density lipoprotein cholesterol in high-fat diet-induced hyperlipidemic rats. The standard drug Niacin showed slightly better effects. The treatment with herbal formulations produced 30-35 percentage improvement in oral glucose tolerance. Similarly all the formulations also reduced the elevated C-reactive protein which is a marker of Hyperlipidemia. In histopathological study it was found that treatment of polyherbal formulation significantly reduced the plaque size in aorta compared with HFD treated control group. The outcome of the study reveals the lipid lowering activity of polyherbal formulations in dyslipidaemic conditions by interfering with the biosynthesis of cholesterol and utilization of lipids.

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Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

Human & Experimental Toxicology ◽

10.1177/0960327111417907 ◽

2011 ◽

Vol 31 (6) ◽

pp. 565-573 ◽

Cited By ~ 13

Author(s):

M Tutanc ◽

V Arica ◽

N Yılmaz ◽

A Nacar ◽

I Zararsiz ◽

...

Keyword(s):

Oxidative Stress ◽

Cyclosporin A ◽

Protective Role ◽

Control Group ◽

Albino Rats ◽

Protective Mechanism ◽

Antioxidant Parameters ◽

Group 2 ◽

Wistar Albino Rats

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

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Evaluation of antidiabetic activity of fruit of Coriandrum sativum. Linn methanolic extract in Streptozocin induced diabetic wistar Albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20175686 ◽

2017 ◽

Vol 7 (1) ◽

pp. 121

Author(s):

Ahmed S. K. ◽

Chakrapani Cheekavolu ◽

Sampath D. ◽

Sunil M.

Keyword(s):

Research Study ◽

Diabetes Management ◽

Methanolic Extract ◽

Coriandrum Sativum ◽

Antidiabetic Activity ◽

Control Group ◽

Albino Rats ◽

Citrate Buffer ◽

Standard Drug ◽

Wistar Albino Rats

Background: Diabetes prevalence is estimated to increase annually. Numerous people use traditional medicine, such as India also considered as the diabetic capital in the world. Diabetes is a metabolic disorder characterized by disturbances in lipid, carbohydrate and protein metabolism. The present study to evaluate the antidiabetic potential of coriandrum sativum. linn fruits methanolic extract in streptozocin induced diabetic wistar albino rats model.Methods: Diabetes induction in wistar albino rats by administration of streptozocin (50mg/kg, i.p.) in citrate buffer. 30 wistar albino rats were divided into 5 groups (A, B, C, D, E). Group A: served as normal control, whereas Group B: diabetic control, Group C, D methanolic coriandrum sativum Linn. fruits extract (CSFME) at a dose of 100, 200mg/kg orally, Group E was given standard drug Glibenclamide (0.5mg/kg) orally. All groups are administered for the period of 14 consecutive days and blood sugar levels was measured at regular intervals up to end of the study.Results: This present research study confirms that the test drug compound CSFME has sustained oral hypoglycaemic activity and statistically significant (p ≤0.05) and which is comparable with standard drug Glibenclamide.Conclusions: This research study confirms that the CSFME has antidiabetic activity against streptozocin induced wistar diabetic albino rats. It could be a novel antidiabetic agent and also a dietary adjunct in the type 2 diabetes management and its complication. Further studies are necessary required to confirm the antidiabetic activity of individual phytochemical compounds of Coriandrum sativum.

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Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

10.21203/rs.3.rs-17918/v1 ◽

2020 ◽

Author(s):

Cecilia Gabriela Meléndez-Salcido ◽

Katya Vargas-Ortiz ◽

Oscar Gerardo Silva-Gaona ◽

María Cristina León-García ◽

Luz Arcelia Ortega-Hernández ◽

...

Keyword(s):

Gene Expression ◽

Fatty Acids ◽

Beneficial Effect ◽

Therapeutic Potential ◽

Cardiometabolic Disease ◽

Control Group ◽

Standard Diet ◽

High Fructose Diet ◽

High Fructose ◽

Fatty Acids Metabolism

Abstract Background: It has been proposed that curcumin modulates the gene expression of different signaling pathways, improve the fatty acids metabolism and exerts a potential beneficial effect on cardiometabolic disease, but this has not been thoroughly demonstrated. In the present study, we evaluated the effect of curcumin upon the expression of PPARα, CPT1, MCAD, VLCAD and ACAA2 in heart of mice fed a high-fructose diet (HFD). Methods: Twenty-four mice C57BL/6 were divided into four groups (n=6) and treated for 15 weeks. Control group (C) received standard diet (SD), Curcumin group (C+Cur), Fructose group (F) and Fructose with Curcumin group (F+Cur). The groups were treated with 0.75% w/w curcumin mixed in the SD and 30% w/v fructose in water, respectively. Heart proteins expression were analyzed by Western Blot. Results: Curcumin supplementation increased PPARα and ACAA2 expression and decreased CPT1 and MCAD expression in heart of mice fed a HFD. However, it did not modify the VLCAD expression. Conclusions: Curcumin regulated PPARα, CPT1 and MCAD expression and increased ACAA2 expression; suggesting a therapeutic potential in the prevention of alterations in mitochondrial fatty acids metabolism in heart of mice fed a HFD.

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Effect of Curcuma longa (Turmeric) on serum creatine kinase-MB and troponin I in isoproterenol induced myocardial infarction in wistar albino rats

Journal of Bangladesh Society of Physiologist ◽

10.3329/jbsp.v13i2.39477 ◽

2018 ◽

Vol 13 (2) ◽

pp. 47-53

Author(s):

Sharmin Nahar ◽

Qazi Shamima Akhter

Keyword(s):

Myocardial Infarction ◽

Body Weight ◽

Troponin I ◽

Curcuma Longa ◽

Heart Weight ◽

Control Group ◽

Albino Rats ◽

Creatine Kinase Mb ◽

The Mean ◽

Wistar Albino Rats

Background: The prevalence of myocardial infarction (MI) is increasing day by day in Bangladesh due to socioeconomic transition. Spices and herbs are important source of remedy for various diseases in human. Curcuma longa suggested to be used as an indigenous medicine for the prevention and treatment of cardiovascular disease. Objective: To observe the effect of Curcuma longa in isoproterenol induced myocardial infarction in Wistar albino rats. Methods: This experimental study was carried out in the Department of Physiology, Dhaka Medical College, Dhaka during 2015. Twenty one Wistar albino male rats, weighing 100 to 150 g (initial body weight); aged 85 to 100 days were selected for the study. After acclimatization for 14 days, the rats were divided into BC (Baseline control group), ISP-TC (Isoproterenol treated control group) and CLP-ISPT (Curcuma longa pretreated and isoproterenol treated group). Each group consisted of 7 rats. After experiment, on the 10th day, final body weight was taken, rats were sacrificed and blood samples were collected from the heart. The heart was removed and weighed. Serum creatine kinase-MB (CK-MB) level was estimated by ELISA method and Troponin I (cTnI) level by AxSYM method. The statistical analysis was done by one way ANOVA and Bonferroni test as applicable. Results: In this study, the mean percent (%) change of body weight (p<0.01), mean serum CK-MB (p<0.001) and cTnI (p<0.001) levels were significantly higher but mean heart weight was non significantly higher in ISP-TC in comparison to those of BC. Again, the mean percent (%) change of body weight (p<0.01), mean heart weight (p<0.01), mean serum CK-MB (p<0.01) and cTnI (p<0.001) levels were significantly lower in CLP-ISPT than those of ISP-TC group. Conclusion: From the results, it can be concluded that Curcuma longa may have cardioprotective effect. J Bangladesh Soc Physiol. 2018, December; 13(2): 47-53

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Dietary Supplementation with Chestnut (Castanea sativa) Reduces Abdominal Adiposity in FVB/n Mice: A Preliminary Study

Biomedicines ◽

10.3390/biomedicines8040075 ◽

2020 ◽

Vol 8 (4) ◽

pp. 75 ◽

Cited By ~ 1

Author(s):

Pedro Rodrigues ◽

Tiago Ferreira ◽

Elisabete Nascimento-Gonçalves ◽

Fernanda Seixas ◽

Rui Miguel Gil da Costa ◽

...

Keyword(s):

Adipose Tissue ◽

Castanea Sativa ◽

Control Group ◽

Standard Diet ◽

Physiological Variables ◽

Lower Serum Cholesterol ◽

Preliminary Study ◽

Castanea Sativa Miller ◽

Old Mice ◽

And Oxidative Stress

The production of chestnut (Castanea sativa Miller) is mostly concentrated in Europe. Chestnut is recognized by its high content of antioxidants and phytosterols. This work aimed to evaluate the effects of dietary chestnut consumption over physiological variables of FVB/n mice. Eighteen FVB/n male 7-month-old mice were randomly divided into three experimental groups (n = 6): 1 (control group) fed a standard diet; 2 fed a diet supplemented with 0.55% (w/w) chestnut; and 3 supplemented with 1.1% (w/w) chestnut. Body weight, water, and food intake were recorded weekly. Following 35 days of supplementation, the mice were sacrificed for the collection of biological samples. Chestnut supplementation at 1.1% reduced abdominal adipose tissue. Lower serum cholesterol was also observed in animals supplemented with chestnut. There were no significant differences concerning the incidence of histological lesions nor in biochemical markers of hepatic damage and oxidative stress. These results suggest that chestnut supplementation may contribute to regulate adipose tissue deposition.

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Can N-Acetylcysteine Preserve Peritoneal Function and Morphology in Encapsulating Peritoneal Sclerosis?

Peritoneal Dialysis International ◽

10.1177/089686080902902s41 ◽

2009 ◽

Vol 29 (2_suppl) ◽

pp. 202-205 ◽

Cited By ~ 8

Author(s):

Devrim Bozkurt ◽

Ender Hur ◽

Burcu Ulkuden ◽

Murat Sezak ◽

Hasim Nar ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Cell Count ◽

Degradation Products ◽

Isotonic Saline ◽

Control Group ◽

Albino Rats ◽

Peritoneal Fibrosis ◽

Beneficial Effects ◽

Group 2 ◽

Wistar Albino Rats

Long-term use of the peritoneum as a dialysis membrane results in progressive irreversible dysfunction, described as peritoneal fibrosis. Oxidative stress during peritoneal dialysis has been established in many studies. Generation of reactive oxygen species (ROS) by conventional peritoneal dialysis solutions, regardless of whether produced by high glucose, angiotensin II, or glucose degradation products may be responsible for progressive membrane dysfunction. The well-known antioxidant molecule N-acetylcysteine (NAC) is capable of direct scavenging of ROS. The aim of the present study was to investigate the effect of NAC therapy on both progression and regression of encapsulating peritoneal sclerosis (EPS). We divided 49 nonuremic Wistar albino rats into four groups: Control group—2 mL isotonic saline intraperitoneally (IP) daily for 3 weeks; CG group—2 mL/200 g 0.1% chlorhexidine gluconate (CG) and 15% ethanol dissolved in saline injected IP daily for a total of 3 weeks; Resting group—CG (weeks 1 – 3), plus peritoneal resting (weeks 4 – 6); NAC-R group—CG (weeks 1 – 3), plus 2 g/L NAC (weeks 4 – 6). At the end of the experiment, all rats underwent a 1-hour peritoneal equilibration test with 25 mL 3.86% PD solution. Dialysate-to-plasma ratio (D/P) urea, dialysate white blood cell count (per cubic milliliter), ultrafiltration (UF) volume, and morphology changes of parietal peritoneum were examined. The CG group progressed to encapsulating peritoneal sclerosis, characterized by loss of UF, increased peritoneal thickness, inflammation, and ultimately, development of fibrosis. Resting produced advantages only in dialysate cell count; with regard to vascularity and dialysate cell count, NAC was more effective than was peritoneal rest. Interestingly, we observed no beneficial effects of NAC on fibrosis. That finding may be a result of our experimental severe peritoneal injury model. However, decreased inflammation and vascularity with NAC therapy were promising results in regard to membrane protection.

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Release of Non-Esterified Fatty Acids from Adipose Tissue in Normal and Diabetic Rats

Nature ◽

10.1038/1841147a0 ◽

1959 ◽

Vol 184 (4693) ◽

pp. 1147-1147 ◽

Cited By ~ 83

Author(s):

J. WENKEOVÁ ◽

J. PÁV

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Diabetic Rats ◽

Esterified Fatty Acids

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Тhe Impact Of Positive Acceleration (+Gz) on Antioxidant Capacity and Histopathological Alterations in Different Organs and Tissues in Rats

Serbian Journal of Experimental and Clinical Research ◽

10.1515/sjecr-2016-0066 ◽

2017 ◽

Vol 18 (3) ◽

pp. 203-212

Author(s):

Jelena Ristic ◽

Tamara Nikolic ◽

Jovana Jeremic ◽

Isidora Stojic ◽

Snezana Janicijevic-Hudomal ◽

...

Keyword(s):

Gastric Mucosa ◽

Antioxidant Capacity ◽

Total Content ◽

Control Group ◽

Albino Rats ◽

Histopathological Alterations ◽

Antioxidant Enzyme System ◽

Biochemical Systems ◽

Wistar Albino Rats ◽

The Impact

Abstract Since the early 1940s, a significant amount of research has been conducted to describe the impact of the high-G acceleration on the cardiovascular system. The objective of the present study was to examine the role of the antioxidant enzyme system under biodynamic stress in the liver, heart and gastric mucosa in response to high-magnitude +Gz exposure in a rat model. Twenty adult male Wistar albino rats (10 rats per group; 9-11 weeks old, 200-250 g b.w.) were divided into the following two groups: control and G (exposed to a biodynamic stress model under positive (+7 Gz) acceleration for 40 s). The influence of acute biodynamic stress on pro-oxidative parameters in the rat liver (xanthine oxidase (XOD), catalase (CAT), peroxidase (Px), glutathione peroxidase (GSH-Px), total content of glutathione (GSH), lipid peroxidation (LPx)) and on histopathological alterations in the liver, cardiac muscle and gastric mucosa was examined. Biodynamic stress resulting from positive (+7 Gz) acceleration resulted in a highly statistically signifi cant increase of CAT GSH-Px activity compared to the control group. The LPx levels were significantly decreased, but the GSH contents and the activities of other enzymes were not significantly changed. Significant microscopic changes in the liver, heart and gastric mucosa were observed in the G group. These results clearly indicate that +Gz acceleration alters biochemical systems. These alterations in cellular processes may be mediated by influences of hypoxia or ischaemia via changes in the antioxidant capacity.

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Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats

Journal of Pharmaceutics ◽

10.1155/2016/9249040 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Hemant Kumar Nagar ◽

Amit Kumar Srivastava ◽

Rajnish Srivastava ◽

Madan Lal Kurmi ◽

Harinarayan Singh Chandel ◽

...

Keyword(s):

Wound Healing ◽

Ethanolic Extract ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Albino Rats ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect ◽

Wistar Albino Rats

Objectives. The present study was aimed at investigating the wound healing effect of ethanolic extract of Cestrum nocturnum (L.) leaves (EECN) using excision and incision wound model. Methods. Wistar albino rats were divided into five groups each consisting of six animals; group I (left untreated) considered as control, group II (ointment base treated) considered as negative control, group III treated with 5% (w/w) povidone iodine ointment (Intadine USP), which served as standard, group IV treated with EECN 2% (w/w) ointment, and group V treated with EECN 5% (w/w) ointment were considered as test groups. All the treatments were given once daily. The wound healing effect was assessed by percentage wound contraction, epithelialization period, and histoarchitecture studies in excision wound model while breaking strength and hydroxyproline content in the incision wound model. Result. Different concentration of EECN (2% and 5% w/w) ointment promoted the wound healing activity significantly in both the models studied. The high rate of wound contraction (P<0.001), decrease in the period for epithelialization (P<0.01), high skin breaking strength (P<0.001), and elevated hydroxyproline content were observed in animal treated with EECN ointments when compared to the control and negative control group of animals. Histopathological studies of the EECN ointments treated groups also revealed the effectiveness in improved wound healing. Conclusions. Ethanolic extract of Cestrum nocturnum (EECN) leaves possesses a concentration dependent wound healing effect.

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