scholarly journals DETECTION OF AEROLYSIN (aerA) GENE IN AEROMONAS HYDROPHILA STRAINS ISOLATED FROM DISEASED CARP

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Vladimir Radosavljević ◽  
Miroslav Ćirković ◽  
Dragana Ljubojević ◽  
Nikolina Novakov ◽  
Đorđe Cvetojević ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Pcr Amplification ◽  
Sudden Increase ◽  
Extracellular Lipase ◽  
Extracellular Proteases ◽  
Hemolytic Toxin ◽  
One Year ◽  
The One ◽  
Motile Aeromonas Septicaemia ◽  
Common Infection

Bacterial septicemia caused by motile aeromonads is common infection in the intensive fish production. Aeromonas (A.) hydrophila is often present in fish populations. Ubiquitous distribution of these bacteria in the aquatic environment, and the stress caused by intensive breeding are predisposing factors for the occurence of the disease. A. hydrophila is considered a major cause of septicaemia caused by motile aeromonads. Several A. hydrophila extracellular products (ECP) are considered as important factors in pathogenesis, primarily aerolysin (aerA), the extracellular lipase, cytolytic enterotoxin, hemolytic toxin and extracellular proteases. PCR detection of aerolysin (aerA) is considered a reliable method of identifying potentially pathogenic Aeromonas strains. In spring 2012, after a sudden increase in water temperature, disease occured in common carp population in one fish farm in Serbia. Five specimens of the one-year-old carp with clinical symptoms of motile aeromonas septicaemia were used for isolation of the bacteria. Identification of A. hydrophila was done on the basis of morphological, physiological, cultural and biochemical characteristics. PCR amplification of DNA from A. hydrophila isolates revealed presence of aerolysin (aerA) gene in all examined A. hydrophila isolates from carp with motile aeromonas septicaemia.

Genetic of Sporadic Hemophagocytic Lymphohistiocytosis

Blood ◽  
2019 ◽  
Vol 134 (Supplement_1) ◽  
pp. 82-82
Author(s):  
Coralie Bloch ◽  
Jean-Philippe Jais ◽  
Marine Gil ◽  
Marouane Boubaya ◽  
Felipe Suarez ◽  
...  
Keyword(s):  
Hemophagocytic Lymphohistiocytosis ◽  
Research Funding ◽  
Clinical Symptoms ◽  
Ferritin Level ◽  
Genetic Component ◽  
Trend Test ◽  
Biological Features ◽  
Associated Diseases ◽  
One Year ◽  
The One

Background: Hemophagocytic lymphohistiocytosis (HLH) syndrome is due to an abnormal and sustained activation of T lymphocytes and macrophages releasing pro-inflammatory cytokines in the microenvironment. HLH is characterized by clinical and biological features that includes fever, hepatomegaly, splenomegaly, high ferritin level, pancytopenia and liver biology abnormalities. HLH is commonly divided into genetic/hereditary cases (HLHg) and non genetic/ sporadic (HLHs) with no hints for an inherited causes. However, similarity of both clinical symptoms and biological abnormalities between HLHg and HLHs raised, however, the hypothesis that HLHs could have a genetic component that may be related to HLHg. However contribution of genetic factors in both, pathophysiology and prognosis of sporadic hemophagocytic lymphohistiocytosis (HLHs) remains unknown. To explore the hypothesis of a genetic susceptibility in HLHs, from 2010 to 2017, we constituted a cohort of 205 HLHs patients (adults and children) and analysed genes involved in genetic HLH (HLHg). Methods: Patients fulfillied modified HLH-2004 diagnostic criteria. Data collected included (i) clinical and biological features, (ii) associated diseases (AID), (iii) infectious events, (iv) treatment, and (v) clinical outcome during one year of follow up. HLHg related genes LYST, PRF1, UNC13-D, STX11, STXBP2, RAB27A, SH2D1A, XIAP were NGS sequenced in 172 patients. Selected variants were (i) unknown but met at least two criteria among MAF<0.05, CADD >10, and/or Polyphen HDIV (>0.485) (n=50), (ii) previously related to HLHg (n=2), PRF1 A755G/N252S (MAF <0.05) and PRF1 C272T/A91V (MAF <0.05, CADD >10 and Polyphen HDIV). Two control cohorts were selected, the first one using the variant files from the European subpopulation of the 1000 Genomes (1000G cohort, n=502) phase 3 project and the second one from the local in-house variants data base (in-house cohort, n=5092). Results: Patients presented with neoplasia (n=62), AID (n=46) in which infection was mainly Herpes virus (EBV) (> 80%), or no etiology (idiopathic) (n=64) with a greater infectious diversity. Fifty-two variants met the selection criteria, they were distributed overall in 115 alleles in 84/172 patients. Variants were globally heterozygous. They were recurrent with occurrence in 2 patients for STX11, RAB27A and LYST genes to 10 patients for the N252S and 18 patients for the p.Ala91Val in PRF1. Overall the frequency of individuals exhibiting at least one variant was significantly in excess (48.8%, n=84) in the HLHs versus in in-house (35.9%, n=1826) (p=0.001, CAST test) or versus in 1000G population patients (38.6%, n=194) (p=0.02, CAST test). Importantly, the distribution of patients carrying either 0, 1, 2, 3 or 4 mutated alleles in HLHs cohort was statistically different from the two control populations (Trend test: HLH vs in-house p<0.0001 and HLH vs 1000G p<0.001; SUM test: HLH vs in-house p<0.001 and HLH vs 1000G p=0.001). We observed an excess of patients carrying one or two mutated in the HLHs cohort vs in-house OR (1 allele vs 0 allele) =1.49 [IC 1.05-2.11]; (2 alleles vs 0 allele) =2.52 [IC 1.54-4.00] and vs 1000G OR 1 allele vs 0 allele =1.27 [IC 0.85-1.88]; 2 alleles vs 0 allele =2.76 [IC 1.50-5.05]. Finally, PRF1/RAB27Aa and PRF1/STXBP2 were found to be positively associated with a highier frequency in HLHs group than expected from a random distribution (p<0.01 and p=0.05, respectively). Importantly, severe patients carried a significantly higher number of variants (1, 2 or 3) than the one with a better outcome (p<0.001, Fisher test exact). A multivariate analysis including sex, age, associated diseases, and infectious status as confounding factors, confirm that the number of HLH genes variants is correlated to the severity of the syndrome independently of these others factors OR(S/NS; 1 variant/0 variant)=3.03 [1.45-6.32] (p=0.003) and OR(S/NS; 2 variants/0 variant) =4.55[1.61-12.84] (p=0.004). Conclusion: The results presented in this study strongly support the hypothesis that HLHs has a genetic component when tested with genes of HLH. Overall, this results established clearly a genotype/phenotype correlation between presence of one or more filtered HLH genes variants and severity of the HLH syndrome in patients. Finally, we proposed a di/tri genic model as an expression of genetic component in sporadic HLH. Disclosures Hermine: Novartis: Research Funding; AB science: Consultancy, Equity Ownership, Honoraria, Research Funding; Celgene: Research Funding.

scholarly journals CARDIOSYNCHRONOUS ELECTROMYOSTIMULATION IN PATIENTS WITH ACUTE HEART FAILURE

2013 ◽  
Vol 12 (6) ◽  
pp. 15-20
Author(s):  
G. G. Ivanov ◽  
M. Yu. Orkvasov ◽  
G. Khalabi
Keyword(s):  
Heart Failure ◽  
Conservative Treatment ◽  
Acute Heart Failure ◽  
Clinical Symptoms ◽  
Impedance Measurement ◽  
Pharmacological Therapy ◽  
Group I ◽  
Group Ii ◽  
One Year ◽  
The One

Aim. To study the effectiveness of the external cardiosynchronous electromyostimulation (ECSEMS) in patients with different variants of acute heart failure (AHF).Material and methods. The study included 62 AHF patients: Group I — those receiving only standard pharmacological therapy; and Group II — those in whom standard pharmacological therapy was ineffective after 12 hours, and who were also administered ECSEMS sessions for the next 7 days. The examination included multi-frequency bio-impedance measurement and dispersion mapping.Results. Clinical symptoms were less severe in Group I. In 64% of the Group II patients, the combination treatment was associated with a positive dynamics of the water balance parameters. The 7-day ECSEMS, as a part of the complex management of AHF patients, significantly reduced the one-month lethality, although did not change the one-year survival.Conclusion. The ECSEMS method could be used in order to increase the effectiveness of the conservative treatment of AHF patients. 

Rapid regression of macroprolactinomas by the new dopamine partial agonist terguride

1986 ◽  
Vol 111 (4) ◽  
pp. 460-466 ◽  
Author(s):  
K.-J. Gräf ◽  
D. Köhler ◽  
R. Horowski ◽  
R. Dorow
Keyword(s):  
Clinical Symptoms ◽  
Partial Agonist ◽  
Imaging Techniques ◽  
Tumour Mass ◽  
Residual Tumour ◽  
Good Tolerance ◽  
Magnetic Resonance Imaging Mri ◽  
One Year ◽  
The One

Abstract. Two patients with macroprolactinomas were treated with the partial dopamine agonist, terguride. The prolactin (Prl) levels were lowered very effectively and in both cases the clinical symptoms improved markedly during the first days of treatment. Computerized tomography (CT) and magnetic resonance imaging (MRI) follow-up studies showed distinct tumour shrinkages which were first documented by MRI within 2 weeks of treatment. Tumour residues were, however, still demonstrable by MRI after more than one year respectively 3 months of therapy. In principal, results from both imaging techniques were comparable with the exception of the one year follow-up study of patient 1. In CT no residual tumour mass was visible whereas MRI showed only little reduction when compared to the 30th week scan. Throughout the treatment terguride was well tolerated without any side effects up to a maximal daily dosage of 3 mg given orally. Presumably the partial agonistic features of terguride contributed to the good tolerance of the treatment as compared to that of full dopamine agonists like bromocriptine or lisuride. Thus, these preliminary results indicate that terguride may be a beneficial alternative in the treatment of prolactinomas and other hyperprolactinaemic states.

CURRENT ASPECTS OF DRUG THERAPY OF CHRONIC HEART FAILURE SYNDROME

2020 ◽  
Vol 4 (2) ◽  
pp. 1085-1096
Author(s):  
T.V. Statkevich ◽  
◽  
N.P. Mitkovskaya ◽  
◽  
Keyword(s):  
Quality Of Life ◽  
Heart Failure ◽  
Drug Therapy ◽  
Chronic Heart Failure ◽  
Clinical Symptoms ◽  
Nyha Class ◽  
Treatment And Prevention ◽  
One Year ◽  
The One

Chronic heart failure (CHF) is an important problem for the country, which has both medical and socio-economic aspects. The presence of the syndrome not only significantly increases the risks of an unfavorable course of diseases underlying its etiological basis, but in itself, through the development of decompensation, causes a high frequency of deaths. Despite all the advances in pharmacotherapy, the prognosis of heart failure remains poor. More than 40% of patients die within 4 years after the diagnosis of heart failure, and the one-year mortality rate for patients with severe CHF (NYHA class IV) exceeds 50%. The foregoing determines the need and importance of using all possible drug and non-drug therapy technologies aimed at reducing mortality, increasing the duration and quality of life of patients with CHF, as well as reducing the number and likelihood of decompensation and related hospitalizations, and makes this direction one of the priorities in medicine. The article describes current approaches to the treatment of patients with CHF syndrome from the perspective of evidence-based medicine and taking into account the recommendations of leading international organizations for the treatment and prevention of cardiovascular diseases. The drugs used were analyzed in terms of their influence on clinical symptoms, quality of life of patients, the risk of hospitalization due to decompensation of CHF, and mortality rates. The emphasis is made on the possibilities, mechanism of action and further prospects for the use of a new class of drugs in the treatment of CHF, acting at the level of the renin-angiotensin-aldosterone system and the system of neutral endopeptidases - inhibitors of angiotensin-neprilisin receptors.

Resources: Internet Resources for the Independent Medical Evaluator – 2007

2007 ◽  
Vol 12 (4) ◽  
pp. 4-7
Author(s):  
Christopher R. Brigham ◽  
Jenny Walker
Keyword(s):  
Head Trauma ◽  
Clonic Seizure ◽  
Acute Subdural Hematoma ◽  
Orbital Fracture ◽  
Orbital Wall ◽  
First Occurrence ◽  
One Year ◽  
The One ◽  
Neurological Injuries ◽  
Medial Orbital Wall

Abstract Rating patients with head trauma and multiple neurological injuries can be challenging. The AMA Guides to the Evaluation of Permanent Impairment (AMA Guides), Fifth Edition, Section 13.2, Criteria for Rating Impairment Due to Central Nervous System Disorders, outlines the process to rate impairment due to head trauma. This article summarizes the case of a 57-year-old male security guard who presents with headache, decreased sensation on the left cheek, loss of sense of smell, and problems with memory, among other symptoms. One year ago the patient was assaulted while on the job: his Glasgow Coma Score was 14; he had left periorbital ecchymosis and a 2.5 cm laceration over the left eyelid; a small right temporoparietal acute subdural hematoma; left inferior and medial orbital wall fractures; and, four hours after admission to the hospital, he experienced a generalized tonic-clonic seizure. This patient's impairment must include the following components: single seizure, orbital fracture, infraorbital neuropathy, anosmia, headache, and memory complaints. The article shows how the ratable impairments are combined using the Combining Impairment Ratings section. Because this patient has not experienced any seizures since the first occurrence, according to the AMA Guides he is not experiencing the “episodic neurological impairments” required for disability. Complex cases such as the one presented here highlight the need to use the criteria and estimates that are located in several sections of the AMA Guides.

Catheter-directed foam sclerotherapy treatment of saphenous vein incompetence

VASA ◽  
2012 ◽  
Vol 41 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Asciutto ◽  
Lindblad
Keyword(s):  
Saphenous Vein ◽  
Short Term ◽  
Foam Sclerotherapy ◽  
Results Of Treatment ◽  
Ulcer Healing Rate ◽  
Complete Obliteration ◽  
Ceap Classification ◽  
One Year ◽  
The One

Background: The aim of this study is to report the short-term results of catheter-directed foam sclerotherapy (CDFS) in the treatment of axial saphenous vein incompetence. Patients and methods: Data of all patients undergoing CDFS for symptomatic primary incompetence of the great or small saphenous vein were prospectively collected. Treatment results in terms of occlusion rate and patients’ grade of satisfaction were analysed. All successfully treated patients underwent clinical and duplex follow-up examinations one year postoperatively. Results: Between September 2006 and September 2010, 357 limbs (337 patients) were treated with CDFS at our institution. Based on the CEAP classification, 64 were allocated to clinical class C3 , 128 to class C4, 102 to class C5 and 63 to class C6. Of the 188 patients who completed the one year follow up examination, 67 % had a complete and 14 % a near complete obliteration of the treated vessel. An ulcer-healing rate of 54 % was detected. 92 % of the patients were satisfied with the results of treatment. We registered six cases of thrombophlebitis and two cases of venous thromboembolism, all requiring treatment. Conclusions: The short-term results of CDFS in patients with axial vein incompetence are acceptable in terms of occlusion and complications rates.

Regional household poverty and mobility analysis – a transition probability approach

2020 ◽  
Vol 63 (3) ◽  
pp. 286-302
Author(s):  
Damian Mowczan ◽  
Keyword(s):  
Transition Probability ◽  
General Level ◽  
Transition Matrices ◽  
Equivalent Unit ◽  
Central Statistical ◽  
Per Capita ◽  
Probability Matrices ◽  
General Mobility ◽  
One Year ◽  
The One

The main objective of this paper was to estimate and analyse transition-probability matrices for all 16 of Poland’s NUTS-2 level regions (voivodeship level). The analysis is conducted in terms of the transitions among six expenditure classes (per capita and per equivalent unit), focusing on poverty classes. The period of analysis was two years: 2015 and 2016. The basic aim was to identify both those regions in which the probability of staying in poverty was the highest and the general level of mobility among expenditure classes. The study uses a two-year panel sub-sample of unidentified unit data from the Central Statistical Office (CSO), specifically the data concerning household budget surveys. To account for differences in household size and demographic structure, the study used expenditures per capita and expenditures per equivalent unit simultaneously. To estimate the elements of the transition matrices, a classic maximum-likelihood estimator was used. The analysis used Shorrocks’ and Bartholomew’s mobility indices to assess the general mobility level and the Gini index to assess the inequality level. The results show that the one-year probability of staying in the same poverty class varies among regions and is lower for expenditures per equivalent units. The highest probabilities were identified in Podkarpackie (expenditures per capita) and Opolskie (expenditures per equivalent unit), and the lowest probabilities in Kujawsko-Pomorskie (expenditures per capita) and Małopolskie (expenditures per equivalent unit). The highest level of general mobility was noted in Małopolskie, for both categories of expenditures.

From Scarcity to Abundance: Achieving Quality-Assured Mass Higher Education

2011 ◽  
Vol 15 (2) ◽  
Author(s):  
Peter P. Smith
Keyword(s):  
Higher Education ◽  
Knowledge Economy ◽  
Good Practice ◽  
The United States ◽  
The Other ◽  
Secondary System ◽  
Mass Higher Education ◽  
Post Secondary ◽  
One Year ◽  
The One

The United States is in a bind. On the one hand, we need millions of additional citizens with at least one year of successful post-secondary experience to adapt to the knowledge economy. Both the Gates and Lumina Foundations, and our President, have championed this goal in different ways. On the other hand, we have a post-secondary system that is trapped between rising costs and stagnant effectiveness, seemingly unable to respond effectively to this challenge. This paper analyzes several aspects of this problem, describes changes in the society that create the basis for solutions, and offers several examples from Kaplan University of emerging practice that suggests what good practice might look like in a world where quality-assured mass higher education is the norm.

Tennis- und Golfer-Ellenbogen

2015 ◽  
Vol 63 (4) ◽  
Keyword(s):  
Spontaneous Recovery ◽  
Clinical Symptoms ◽  
Lateral Epicondylitis ◽  
High Rate ◽  
Evidence Based ◽  
Physical Methods ◽  
Local Injections ◽  
One Year ◽  
Golfer’S Elbow ◽  
Non Surgical Treatment

“Tennis and golfer’s elbow” are common pathologies due to overload of forearm extensors and flexors, and actually occur mostly outside tennis and golf sports. Several differential diagnoses of medial and lateral epicondylitis have to be excluded as there are a number of other conditions with similar clinical symptoms. The high rate of spontaneous recovery has to be considered in treatment. Evidence based conservative treatment comprises excentric physiotherapy, local injections, and physical methods. Surgery is reserved for patients with persistence of symptoms for more than one year despite non-surgical treatment.

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