STUDY OF METHYLENE BLUE INTERACTION WITH SYNTHETIC POLYNUCLEOTIDE POLY(rA)-POLY(rU)

2020 ◽  
Vol 54 (2 (252)) ◽  
pp. 112-117
Author(s):  
M.A. Parsadanyan ◽  
M.A. Shahinyan ◽  
A.P. Antonyan

A study of the interaction of methylene blue (MB) with poly(rA)-poly(rU) by the method of fluorescence spectroscopy has been carried out. The data obtained revealed that MB, being a DNA-specific ligand, can bind to double-stranded regions of RNA. In this regard, as in the case of DNA, semi-intercalation was the most preferable mechanism for the binding of this ligand to poly(rA)-poly(rU). On the other hand, non-linear curves of dependence of F0/F on concentration of polynucleotide might result from two binding modes, the second of which was probably of an electrostatic nature. Proceeding from the data obtained, the value of KSV was revealed to be almost an order of magnitude less than for DNA, which may indicate that RNA is a less preferable target for MB.

1994 ◽  
Vol 59 (6) ◽  
pp. 1439-1450 ◽  
Author(s):  
Miroslava Žertová ◽  
Jiřina Slaninová ◽  
Zdenko Procházka

An analysis of the uterotonic potencies of all analogs having substituted L- or D-tyrosine or -phenylalanine in position 2 and L-arginine, D-arginine or D-homoarginine in position 8 was made. The series of analogs already published was completed by the solid phase synthesis of ten new analogs having L- or D-Phe, L- or D-Phe(2-Et), L- or D-Phe(2,4,6-triMe) or D-Tyr(Me) in position 2 and either L- or D-arginine in position 8. All newly synthesized analogs were found to be uterotonic inhibitors. Deamination increases both the agonistic and antagonistic potency. In the case of phenylalanine analogs the change of configuration from L to D in position 2 enhances the uterotonic inhibition for more than 1 order of magnitude. The L to D change in position 8 enhances the inhibitory potency negligibly. Prolongation of the side chain of the D-basic amino acid in position 8 seems to decrease slightly the inhibitory potency if there is L-substituted amino acid in position 2. On the other hand there is a tendency to the increase of the inhibitory potency if there is D-substituted amino acid in position 2.


Author(s):  
Diego Liberati

In many fields of research, as well as in everyday life, it often turns out that one has to face a huge amount of data, without an immediate grasp of an underlying simple structure, often existing. A typical example is the growing field of bio-informatics, where new technologies, like the so-called Micro-arrays, provide thousands of gene expressions data on a single cell in a simple and fast integrated way. On the other hand, the everyday consumer is involved in a process not so different from a logical point of view, when the data associated to his fidelity badge contribute to the large data base of many customers, whose underlying consuming trends are of interest to the distribution market. After collecting so many variables (say gene expressions, or goods) for so many records (say patients, or customers), possibly with the help of wrapping or warehousing approaches, in order to mediate among different repositories, the problem arise of reconstructing a synthetic mathematical model capturing the most important relations between variables. To this purpose, two critical problems must be solved: 1 To select the most salient variables, in order to reduce the dimensionality of the problem, thus simplifying the understanding of the solution 2 To extract underlying rules implying conjunctions and/or disjunctions between such variables, in order to have a first idea of their even non linear relations, as a first step to design a representative model, whose variables will be the selected ones When the candidate variables are selected, a mathematical model of the dynamics of the underlying generating framework is still to be produced. A first hypothesis of linearity may be investigated, usually being only a very rough approximation when the values of the variables are not close to the functioning point around which the linear approximation is computed. On the other hand, to build a non linear model is far from being easy: the structure of the non linearity needs to be a priori known, which is not usually the case. A typical approach consists in exploiting a priori knowledge to define a tentative structure, and then to refine and modify it on the training subset of data, finally retaining the structure that best fits a cross-validation on the testing subset of data. The problem is even more complex when the collected data exhibit hybrid dynamics, i.e. their evolution in time is a sequence of smooth behaviors and abrupt changes.


1997 ◽  
Vol 482 ◽  
Author(s):  
K. P. Koron ◽  
A. Wysmolek ◽  
J. M. Baranowskil ◽  
K. Pakul ◽  
J. P. Bergman ◽  
...  

AbstractPhotoluminescence connected with excitons and their phonon replicas in undoped homoepitaxial MOCVD grown GaN layers have been studied in the temperature range 2 - 100 K. It is shown that the coupling between LO phonons and neutral acceptor bound excitons (ABE) is much stronger than the coupling between LO phonons and neutral donor bound excitons (DBE). In spite that emission due the DBE no-phonon is one order of magnitude stronger than the ABE one, the predominant feature of the first LO phonon replica of the excitonic structures is related to the ABE. It is argued that this fact is connected with delocalization of the acceptor wavefunction in the k-space which leads to a higher number of interacting LO phonons in the first replica. On the other hand, the second LO phonon replica of the excitonic structures is predominantly connected with the DBE. In the case of two LO phonons interacting with bound excitons the k - conservation has not so direct influence. In addition, the temperature dependence of LO phonon replicas and their kinetics in ps regime are also reported.


Author(s):  
A. BURRIEZA ◽  
E. MUÑOZ-VELASCO ◽  
M. OJEDA-ACIEGO

We introduce the syntax, semantics, and an axiom system for a PDL-based extension of the logic for order of magnitude qualitative reasoning, developed in order to deal with the concept of qualitative velocity, which together with qualitative distance and orientation, are important notions in order to represent spatial reasoning for moving objects, such as robots. The main advantages of using a PDL-based approach are, on the one hand, all the well-known advantages of using logic in AI, and, on the other hand, the possibility of constructing complex relations from simpler ones, the flexibility for using different levels of granularity, its possible extension by adding other spatial components, and the use of a language close to programming languages.


1988 ◽  
Vol 106 (5) ◽  
pp. 1539-1543 ◽  
Author(s):  
T Kohno ◽  
T Shimmen

Pollen tubes show active cytoplasmic streaming. We isolated organelles from pollen tubes and tested their ability to slide along actin bundles in characean cell models. Here, we show that sliding of organelles was ATP-dependent and that motility was lost after N-ethylmaleimide or heat treatment of organelles. On the other hand, cytoplasmic streaming in pollen tube was inhibited by either N-ethylmaleimide or heat treatment. These results strongly indicate that cytoplasmic streaming in pollen tubes is supported by the "actomyosin"-ATP system. The velocity of organelle movement along characean actin bundles was much higher than that of the native streaming in pollen tubes. We suggested that pollen tube "myosin" has a capacity to move at a velocity of the same order of magnitude as that of characean myosin. Moreover, the motility was high at Ca2+ concentrations lower than 0.18 microM (pCa 6.8) but was inhibited at concentration higher than 4.5 microM (pCa 5.4). In conclusion, cytoplasmic streaming in pollen tubes is suggested to be regulated by Ca2+ through "myosin" inactivation.


1984 ◽  
Vol 30 (9) ◽  
pp. 1133-1136 ◽  
Author(s):  
D. Mayrand ◽  
G. Bourgeau ◽  
D. Grenier ◽  
J.-M. Lacroix

Bacteroides endodontalis, a newly described asaccharolytic black-pigmented Bacteroides, along with the other two recognized species of this group (B. gingivalis and B. asaccharolyticus) were studied for their susceptibility to various dyes and inhibitory agents and for some of their enzymatic activities to facilitate differentiating between them. Bacteroides endodontalis resembles B. asaccharolyticus physiologically except for the fact that the former cannot grow on media containing methylene blue, neutral red, or 3% sodium choride, whereas B. asaccharolyticus can. On the other hand, B. endodontalis and B. gingivalis can grow on a medium containing Congo red while B. asaccharolyticus cannot.


2017 ◽  
Vol 19 (36) ◽  
pp. 157
Author(s):  
Lise Bernhardt

This article addresses the concepts of coherence and cohesion in hypertext documents on the Internet. Textual coherence is usually associated with a single, continuous line through the text but the question is whether this also applies to hypertext documents since hypertexts are non-linear. Cohesion, on the other hand, is the grammatical connectedness of utterances and here the question is whether it is at all possible to talk about cohesion in hypertexts since many consider the modules which form the hypertexts as grammatically independent of other modules. Following an intial discussion of these problems I present the kinds of coherence and cohesion phenomena which exist in hypertexts documents.


2009 ◽  
Vol 24 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Koviljka Stankovic ◽  
Milos Vujisic ◽  
Edin Dolicanin

The wide-spread use of semiconductor and gas-filled diodes for non-linear over-voltage protection results in a variety of possible working conditions. It is therefore essential to have a thorough insight into their reliability in exploitation environments which imply exposure to ionizing radiation. The aim of this paper is to investigate the influence of irradiation on over-voltage diode characteristics by exposing the diodes to californium-252 combined neutron/gamma radiation field. The irradiation of semiconductor over-voltage diodes causes severe degradation of their protection characteristics. On the other hand, gas-filled over-voltage diodes exhibit a temporal improvement of performance. The results are presented with the accompanying theoretical interpretations of the observed changes in over-voltage diode behaviour, based on the interaction of radiation with materials constituting the diodes.


2021 ◽  
Vol 70 (2) ◽  
pp. 90-98
Author(s):  
Abigaël Alkema

Let l(u)⊃ |G|. A central problem in higher non-linear graph theoryis the construction of projective numbers. We show that Recent developments in axiomatic set theory [6] have raised the questionof whetherEis not dominated byl. On the other hand, the work in [6, 24] did not consider the hyper-real case.


2020 ◽  
Author(s):  
Laura Palacios-Peña ◽  
Philip Stier ◽  
Raquel Lorente-Plazas ◽  
Pedro Jiménez-Guerrero

Abstract. The impact of aerosol-radiation and aerosol-clouds interactions on the radiative forcing is subject to large uncertainties. This is caused by the limited understanding of aerosol optical properties and the role of aerosols as cloud condensation/ice nuclei (CCN/IN). On the other hand, aerosol optical properties and vertical distribution are highly related and their uncertainties come from different processes. This work attempts to quantify the sensitivity of aerosol optical properties (i.e. aerosol optical depth; AOD) and their vertical distribution (using the extinction coefficient, backscatter coefficient, and concentrations species profiles) to key processes. In order to achieve this objective sensitivity tests have been carried out, using the WRF-Chem regional fully coupled model by modifying the dry deposition, sub-grid convective transport, relative humidity and wet scavenging. The 2010 Russian heatwave/wildfire episode has been selected as case study. Results indicate that AOD is sensitive to these key processes in the following order of importance: 1) modification of relative humidity, causing AOD differences up to 0.6; 2) modification of vertical convection transport with AOD differences around  0.4; and 3) the dry deposition with AOD differences up to −0.35 and 0.3. Moreover, these AOD changes exhibit a non-linear response. Both, an increase and a decrease in the RH result in higher AOD values. On the other hand, both, the increase and offset of the sub-grid convective transport lead to a reduction in the AOD over the fire area. In addition, a similar non-linear response is found when reducing the dry deposition velocity; in particular, for the accumulation mode where the concentration of several species increases (while a decrease might be expected). These non-linear responses are highly dependent on the equilibrium of the thermodynamics system sulphate-nitrate-SOA (secondary organic aerosol). In this sense, small changes in the concentration of one species can strongly affect others, finally affecting aerosol optical properties. Changes in this equilibrium could come from modifications in relative humidity, dry deposition or vertical convective transport. By itself, dry deposition also presents a high uncertainty influencing the AOD representation.


Sign in / Sign up

Export Citation Format

Share Document