scholarly journals Micropropagation of Pelargonium odoratissimum (L.) L’Her. through petioles and leaves

2021 ◽  
Vol 38 (2) ◽  
pp. 261-278
Author(s):  
Asghar Ebrahimzadeh ◽  
Maliheh Fathollahzadeh ◽  
Mohammad Bagher Hassanpouraghdam ◽  
Mohammad Aazami Mavaloo
Keyword(s):  
Peat Moss ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Culture Methods ◽  
Petiole Explants ◽  
The Media ◽  
The Common ◽  
Intact Leaves ◽  
Leaf Segments

Pelargonium odoratissimum (L.) L’Her is a hard rooting plant and the common methods of propagation via stem cuttings are not successful with this species. therefore, tissue culture methods have been experienced for the mass-propagation of this high-valued species. Intact leaves, leaf segments and petiole sections derived from nodal explants in vitro were employed for the optimization of P. odoratissimum micropropagation. The treatment combinations used were MS and 1/2 MS media supplemented with 6-benzylaminopurine, BAP (1, 1.5, 2 and 4.5 mg.L-1) and 1-naphthaleneacetic acid, NAA (0.1, 1 and 1.5 mg.L-1). With leaf segments, the lowest browning incidence, the greatest callogenesis and the highest number of shoots were obtained with the media containing 1.5 mg.L-1 BAP and 1 mg.L-1 NAA. Two mg.L-1 BAP + 0.1 mg.L-1 NAA kept the same results for petiole explants. Intact leaves showed the best results for the three mentioned treatments with 1 mg.L-1 BAP + 1 mg.L-1 NAA. 0.2 mg.L-1 NAA caused the highest rooting percentage and the greatest mean data for the number and length of the roots. Rooted plantlets were transferred to the pots containing 1:1 peat-moss and perlite. Acclimatization of the plantlets was followed by 90 % of survival rate in the greenhouse. The protocol employed would be a potent one to present for the extension section.

scholarly journals Influence of Glucose Concentration on Colony-Forming Efficiency and Biological Performance of Primary Human Tissue–Derived Progenitor Cells

Cartilage ◽  
2020 ◽  
pp. 194760352090660 ◽  
Author(s):  
Venkata P. Mantripragada ◽  
Ryan Kaplevatsky ◽  
Wes A. Bova ◽  
Cynthia Boehm ◽  
Nancy A. Obuchowski ◽  
...  
Keyword(s):  
Glucose Concentration ◽  
Pairwise Comparison ◽  
Culture Methods ◽  
Glucose Levels ◽  
Colony Forming Efficiency ◽  
Forming Efficiency ◽  
The Media ◽  
Outerbridge Grade ◽  
Biological Performance

Objective Glucose concentrations used in current cell culture methods are a significant departure from physiological glucose levels. The study focuses on comparing the effects of glucose concentrations on primary human progenitors (connective tissue progenitors [CTPs]) used for cartilage repair. Design Cartilage- (Outerbridge grade 1, 2, 3; superficial and deep zone cartilage), infrapatellar fatpad-, synovium-, and periosteum-derived cells were obtained from 63 patients undergoing total knee arthroplasty and cultured simultaneously in fresh chondrogenic media containing 25 mM glucose (HGL) or 5 mM glucose (NGL) for pairwise comparison. Automated ASTM-based quantitative image analysis was used to determine colony-forming efficiency (CFE), effective proliferation rates (EPR), and sulfated-proteoglycan (GAG-ECM) staining of the CTPs across tissue sources. Results HGL resulted in increased cell cultures with CFE = 0 compared with NGL in all tissue sources ( P = 0.049). The CFE in NGL was higher than HGL for superficial cartilage ( P < 0.001), and contrary for synovium-derived CTPs ( P = 0.046) when CFE > 0. EPR of the CTPs did not differ between the media in the 6-day assay time period ( P = 0.082). The GAG-ECM area of the CTPs and their progeny was increased in presence of HGL ( P = 0.027). Conclusion Glucose concentration is critical to progenitor’s physiology and should be taken into account in the setting of protocols for clinical or in vitro cell expansion strategies.

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

scholarly journals In vitro Multiple Shoot Regeneration from Petunia hybrida

2019 ◽  
Vol 7 (10) ◽  
pp. 1554
Author(s):  
Rebaz Rasul Habas ◽  
Musa Turker ◽  
Fethi Ahmet Ozdemir
Keyword(s):  
Petunia Hybrida ◽  
Survival Rates ◽  
Basal Medium ◽  
Multiple Shoot ◽  
Shoot Length ◽  
Peat Moss ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Shoot Number

An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.

scholarly journals High Efficiency Direct Shoot Organogenesis from Leaf Segments ofAerva lanata(L.) Juss. Ex Schult by Using Thidiazuron

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
K. Varutharaju ◽  
C. Soundar Raju ◽  
C. Thilip ◽  
A. Aslam ◽  
A. Shajahan
Keyword(s):  
Shoot Organogenesis ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Scale Production ◽  
Ms Medium ◽  
Leaf Segments ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

An efficient protocol for direct shoot organogenesis has been developed for the medicinal plantAerva lanata(L.) Juss. ex Schult. Regeneration was achieved from leaf segments of 20 days oldin vitroplantlets raised on Murashige and Skoog (MS) medium containing 0.25–2.0 mg L−1thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture incubation, maximum number of shoot organogenesis (23.6 ± 0.16) was obtained on medium containing 1.0 mg L−1TDZ. The shoots were able to producein vitroflowers on medium containing 1.0 mg L−1TDZ in combination with 0.25–0.5 mg L−1  α-naphthaleneacetic acid (NAA). Histological observation showed that the epidermal cells of the leaf explants exhibited continuous cell division led to formation of numerous dome shaped meristematic protrusions and subsequently developed into adventitious shoots. Upon transfer of shootlets to half strength MS medium containing 1.0 mg L−1indole-3-butyric acid (IBA), around 86% of the regenerated shoots formed roots and plantlets. Rooted plants were hardened and successfully established in the soil at the survival rate of 92%. The regeneration protocol developed in this study provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for a large scale production of its medicinally active compounds and genetic transformations for further improvement.

scholarly journals Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation

2021 ◽  
Vol 32 (3) ◽  
pp. 1-23
Author(s):  
Siti Madihah Mohd Don ◽  
Nur Maziyyah Abdul Hamid ◽  
Hussein Taha ◽  
Rahayu Sukmaria Sukri ◽  
Faizah Metali
Keyword(s):  
Leaf Explants ◽  
Hormone Treatment ◽  
Stem Cutting ◽  
Ex Situ ◽  
Peat Moss ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Brunei Darussalam ◽  
The Mean ◽  
Propagation Methods

Hoya imperialis (H. imperialis) and H. coronaria (Apocynaceae) are known to have ornamental value due to their beautiful flowers; however, the feasibility of propagating these plants have not been reported despite the wild populations in Brunei Darussalam being highly threatened due to habitat loss and overcollection. Thus, the present study aimed to conduct a preliminary study of the feasibility of two alternative propagation methods, stem cutting and micropropagation, as a potential approach for their ex situ conservation. Hoya stem cuttings were treated with either indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA) (0–2000 mg/L), and then propagated onto a mixture of peat moss and perlite. For micropropagation, Hoya leaf explants were cultured onto Murashige and Skoog (MS) agar media that were supplemented with IBA and/or kinetin (KN) (0–10.0 mg/L). This present study shows that both Hoya species were successfully propagated by stem cutting even without hormone treatment. However, interestingly, in H. imperialis, when compared with control, the mean number of new leaves (6.3 ± 1.0) and the mean relative growth rate (RGR) based on stem diameter (0.004 ± 0.0007 cm cm−1 day−1) significantly increased when treated with 500 mg/L NAA and 2000 mg/L IBA, respectively. Meanwhile, in H. coronaria, significantly higher mean number of roots was achieved by treating with 1000 mg/L NAA (16.6 ± 1.4) or 2000 mg/L IBA (17.5 ± 2.7) compared with control. For micropropagation, callus induction was not promising and could only be observed at specific concentrations of both IBA and KN, with H. imperialis appearing to be more responsive towards these hormones in comparison to H. coronaria. The present study showed that stem cutting appeared more feasible in propagating both Hoya species.

scholarly journals In Vitro Shoot Regeneration and Polyploid Induction of Rhododendron ‘Fragrantissimum Improved’

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 801-804 ◽  
Author(s):  
Cary J. Hebert ◽  
Darren H. Touchell ◽  
Thomas G. Ranney ◽  
Anthony V. LeBude
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Regenerative Capacity ◽  
Leaf Segments ◽  
Ornamental Traits ◽  
Hybrid Protocols ◽  
Mitotic Inhibitor ◽  
In Vitro Shoot Regeneration

Rhododendron L.‘Fragrantissimum Improved’ is an attractive cultivar with showy, fragrant flowers but has limited potential for breeding because it is a sterile wide hybrid. Protocols for in vitro regeneration and polyploid induction were developed for this cultivar as a means to potentially restore fertility and enhance ornamental traits. Combinations of thidiazuron (TDZ) at 0, 5, 10, 15, or 20 μM and 1-naphthaleneacetic acid (NAA) at 0, 2.5, 5, or 10 μM were used to induce shoot regeneration from leaves. Shoot regeneration was optimized (68% of leaf segments produced shoots) using 8.8 μM TDZ and 10 μM NAA. To induce polyploidy, regenerative callus was treated with 7.5, 15, 30, 60, or 90 μM of the mitotic inhibitor oryzalin for 1, 3, 5, 7, or 14 d in various combinations. Oryzalin significantly affected survival and shoot regenerative capacity. A percentage of homogenous, tetraploid shoots was recovered from treatments of 30 μM oryzalin for 1 (13%) or 3 (13%) days and 7.5 μM oryzalin for 7 (20%) or 14 (7%) days.

In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Biologia ◽  
2014 ◽  
Vol 69 (7) ◽  
Author(s):  
Dilek Tekdal ◽  
Selim Cetiner
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Selective Medium ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Plant Parts ◽  
The Family ◽  
The Media ◽  
Thermopsis Turcica

AbstractThis plant tissue study of micropropagation identifies the selective medium saving for rapid propagation in cultivated Thermopsis turcica, an endangered germplasm of the family Fabaceae. The aim is to obtain the optimum growth medium of T. turcica by enabling the in vitro propagation of this endemic. In this study, the leaves and stems of T. turcica were cultured on a Murashige and Skoog’s medium supplemented with various concentrations (0.5, 1.0 and 2.0 mg L−1 1-Naphthaleneacetic acid) of auxin and (0.2 and 0.5 mg L−1 Zeatin) (1.0 and 2.0 mg L−1 Benzylaminopurine) of cytokinins. Previous research focused on the regeneration from the seed of T. turcica Eber population; we concentrated upon the regeneration of different plant parts (leaf and stem) of T. turcica Aksehir population. In addition, according to the literature on T. turcica that to date the effects of Zeatin on the regeneration has not been performed. The most promising regeneration and growth were obtained from leaf explants cultured on the media with 2.0 mg L−1 1-Naphthaleneacetic acid and 0.5 mg L−1Zeatin (93.3%). The regenerated plantles were rooted on the media containing 2.0 mg L−1 Indole-3-butyric acid. Rooted plantlets were transplanted into potting of sterilized soil. The present study reports on the sufficient in vitro regeneration protocol through organogenesis in T. turcica. The findings presented here have implications for in vitro protection and use of this endemic endangered species in further biotechnological research.

scholarly journals Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

2012 ◽  
Vol 12 ◽  
pp. 1-6 ◽  
Author(s):  
Shambhu P. Dhital ◽  
Hak T. Lim ◽  
Hira K. Manandhar
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Solid Medium ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Petiole Explants ◽  
Number Of Shoots

Response of widely grown potato cv. Superior and newly developed cvs. Gui valley and Bora valley to plant growth regulators (PGRs) for direct plant regeneration from internode, leaf blade and petiole explants were investigated. The explants were cultured on a MS solid medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), zeatin, indole-3-acetic acid (IAA) and gibberellic acid (GA3). Potato cv. Superior, regenerated direct shoot without callus and root formation on MS solid medium supplemented with BAP or zeatin, proliferous roots were produced on NAA or IAA supplemented medium and only some calli were produced on GA3 supplemented medium. The regeneration response varied with different concentrations of PGRs, singly and also in combinations. In the case of combined application of PGRs, the highest shoot regeneration (75.3%) and number of shoot per explant (11.5) and number of roots per explant (7.0) were obtained from the MS solid medium supplemented with zeatin (2 mg l-1), NAA (0.1 mg l-1) and GA3 0.1 mg l-1). Among the three types of explants evaluated, internodes produced the highest number of shoots and roots for both potato cvs. Gui valley and Bora valley, and petiole produced the least number of shoots and roots. The regenerated shoots were rooted in PGRs-free MS solid medium and successfully established under glasshouse condition. Leaf, flower, and tuber morphology were identical to in vitro control and mother plants in the same conditions. This optimized regeneration system can be used for rapid shoot proliferation and also for gene transformation.DOI: http://dx.doi.org/10.3126/njst.v12i0.6471 Nepal Journal of Science and Technology 12 (2011) 1-6 

scholarly journals In Vitro Propagation, Genetic Assessment, and Medium-Term Conservation of the Coastal Endangered Species Tetraclinis Articulata (Vahl) Masters (Cupressaceae) from Adult Trees

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 187
Author(s):  
Jorge Juan-Vicedo ◽  
Francisco Serrano-Martínez ◽  
Miriam Cano-Castillo ◽  
José Luis Casas
Keyword(s):  
Basal Medium ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Peat Moss ◽  
Naphthaleneacetic Acid ◽  
Arid Environments ◽  
Multiplication Rate ◽  
Tetraclinis Articulata ◽  
Adult Trees

Tetraclinis articulata (Vahl) Masters is an endangered tree growing in coastal and arid environments that is widely exploited by the timber and resin industry, among other applications. In this context, the use of in vitro techniques is highly encouraged for its propagation. We present a protocol for micropropagation using twigs from adult trees as a source of explants. The Schenk and Hildebrandt basal medium (SH) supplemented with 30 g L−1 sucrose, 6.5 g L−1 plant agar, 4.0 mg L−1 6-benzyladenine (BA), and 0.05 mg L−1 1-naphthaleneacetic acid (NAA) provided the optimum multiplication rate (90.48 ± 9.52 explants with basal shoots and 2.58 ± 0.29 basal shoots per explant). Application of activated charcoal (AC) or ½ Knop solution in a liquid overlay produced significantly longer shoots. Supplementation of solid media with indole-3-butyric acid (IBA) or NAA gave low rooting percentages (<17%). Addition of 0.9 g L−1 AC improved rooting (40%) but rooting performance was optimal (66.7%) after a pulse treatment consisting of 4 h immersion in liquid SH medium without growth regulators, followed by 8 weeks of cultivation. Rooted microplants were successfully acclimatized (93.33%) in a peat moss and vermiculite mixture (1:1 v/v ratio). The genetic stability of the in vitro regenerated plantlets was confirmed using the randomly amplified polymorphic DNA (RAPD) technique. Explant survival and growth remained higher than 90% after 28 weeks of cold storage at both 4 °C and 10 °C. The protocol presented here allows for largescale T. articulata production and could be applied for both ex situ conservation strategies and industrial purposes.

scholarly journals Growth and Development of Lingonberry Cultivars as Affected by In Vitro and Ex Vitro Culture Methods and Source Propagule

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 891A-891 ◽  
Author(s):  
Samir Debnath*
Keyword(s):  
Growth And Development ◽  
Shoot Proliferation ◽  
Lower Number ◽  
Morphological Development ◽  
Multiplication Rate ◽  
Stem Cuttings ◽  
Ex Vitro ◽  
Culture Methods ◽  
In Vitro Shoot Proliferation

The morphological development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants or by shoot regeneration from excised leaves of micropropagated shoots, was studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. In vitro-derived plants produced more shoots branches and rhizomes in contrast to conventional cuttings which rarely produced rhizomes. Plants propagated from cuttings had a lower number but vigorous shoots and thicker rhizomes than in vitro-derived plants. Source propagule had significant effect on multiplication rate. Another experiment evaluated the effect of indole-3-butyric acid (IBA) application to softwood cuttings on subsequent rooting, shoot development, and rhizome production. Treating cuttings with IBA did not significantly improve rhizome formation and elongation. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favored rhizome production. The advantage of rhizome production of in vitro-derived plants over stem cuttings varied among genotypes.

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