Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation

Hoya imperialis (H. imperialis) and H. coronaria (Apocynaceae) are known to have ornamental value due to their beautiful flowers; however, the feasibility of propagating these plants have not been reported despite the wild populations in Brunei Darussalam being highly threatened due to habitat loss and overcollection. Thus, the present study aimed to conduct a preliminary study of the feasibility of two alternative propagation methods, stem cutting and micropropagation, as a potential approach for their ex situ conservation. Hoya stem cuttings were treated with either indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA) (0–2000 mg/L), and then propagated onto a mixture of peat moss and perlite. For micropropagation, Hoya leaf explants were cultured onto Murashige and Skoog (MS) agar media that were supplemented with IBA and/or kinetin (KN) (0–10.0 mg/L). This present study shows that both Hoya species were successfully propagated by stem cutting even without hormone treatment. However, interestingly, in H. imperialis, when compared with control, the mean number of new leaves (6.3 ± 1.0) and the mean relative growth rate (RGR) based on stem diameter (0.004 ± 0.0007 cm cm−1 day−1) significantly increased when treated with 500 mg/L NAA and 2000 mg/L IBA, respectively. Meanwhile, in H. coronaria, significantly higher mean number of roots was achieved by treating with 1000 mg/L NAA (16.6 ± 1.4) or 2000 mg/L IBA (17.5 ± 2.7) compared with control. For micropropagation, callus induction was not promising and could only be observed at specific concentrations of both IBA and KN, with H. imperialis appearing to be more responsive towards these hormones in comparison to H. coronaria. The present study showed that stem cutting appeared more feasible in propagating both Hoya species.

Download Full-text

Shooting of giant reed (Arundo donax L.) stem cuttings in cold greenhouse

International Journal of Plant Biology ◽

10.4081/pb.2016.6294 ◽

2016 ◽

Vol 7 (1) ◽

Cited By ~ 2

Author(s):

Piergiorgio Gherbin ◽

Simone Milan ◽

Giuseppe Mercurio ◽

Antonio Scopa

Keyword(s):

Arundo Donax ◽

Stem Cutting ◽

Giant Reed ◽

Stem Cuttings ◽

Mother Plants ◽

Propagation Methods ◽

One Year ◽

Arundo Donax L

The increasing interest in<em> Arundo donax,</em> a perennial lignocellulosic species only reproducing by propagation, requires the setup of cheap, simple and reliable techniques. Considering these targets, stem cutting offers considerable advantages. The present investigation aimed to compare: i) plants obtained by different propagation methods (by rhizome and micropropagation mother plants); ii) plants obtained by stem cuttings from basal, central and apical parts of the stem; iii) different planting periods (spring, summer, autumn). The obtained results showed that the number of new shoots from stem buds was: i) higher in the spring and lower in the summer planting period; ii) higher from cuttings obtained by micropropagated than rhizome mother plants, both in spring and summer plantings; iii) decreasing passing from the basal to the apical stem portion; iv) partly unexpressed in the autumn planting period; v) lower from one-year stem cuttings as compared to two-year stem cuttings.

Download Full-text

Propagation of Jasminum parkeri: A Critically Endangered Wild Ornamental Woody Shrub from Western Himalaya

Agronomy ◽

10.3390/agronomy11020331 ◽

2021 ◽

Vol 11 (2) ◽

pp. 331

Author(s):

Ujala Kashyap ◽

Anjali Chandel ◽

Diksha Sharma ◽

Sonali Bhardwaj ◽

Bhavya Bhargava

Keyword(s):

Population Decline ◽

Natural Populations ◽

Western Himalaya ◽

Critically Endangered ◽

Ex Situ ◽

Naphthaleneacetic Acid ◽

Western Himalayas ◽

Stem Cuttings ◽

Survival Percentage ◽

Primary Roots

Jasminum parkeri Dunn is a narrowly endemic, critically endangered woody ornamental shrub confined to sub-temperate zone of Western Himalayas, and rediscovered from its type locality after a lapse of about 100 years. In the present study, a propagation technique of J. parkeri, using stem cuttings, was established for the first time through application of auxins, namely, indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA), at varying concentrations ranging from 1000 to 4000 ppm. The highest rooting percentage (98.33% (85.68% ± 4.32)), number of primary roots (36), root length (29.68 cm) and survival percentage (96.67% (83.85% ± 6.16)) were recorded for cuttings treated with 3000 ppm NAA. The phenological comparison between pot plants propagated through seeds and stem cuttings in a naturally ventilated polyhouse revealed a reduction in vegetative and flowering phases in cutting raised plants. Additionally, a noteworthy adaptive behavior of two weeks of early flowering and four weeks of extended flowering (February to October) was observed in plants raised under polyhouse conditions. This method will help in protecting the species from population decline, thereby significantly increasing its potential to be harnessed as an ornamental plant in India. Furthermore, plants grown ex situ will be reintroduced in natural populations.

Download Full-text

Micropropagation of Pelargonium odoratissimum (L.) L’Her. through petioles and leaves

Revista de la Facultad de Agronomía, Universidad del Zulia ◽

10.47280/revfacagron(luz).v38.n2.03 ◽

2021 ◽

Vol 38 (2) ◽

pp. 261-278

Author(s):

Asghar Ebrahimzadeh ◽

Maliheh Fathollahzadeh ◽

Mohammad Bagher Hassanpouraghdam ◽

Mohammad Aazami Mavaloo

Keyword(s):

Peat Moss ◽

Naphthaleneacetic Acid ◽

Stem Cuttings ◽

Culture Methods ◽

Petiole Explants ◽

The Media ◽

The Common ◽

Intact Leaves ◽

Leaf Segments

Pelargonium odoratissimum (L.) L’Her is a hard rooting plant and the common methods of propagation via stem cuttings are not successful with this species. therefore, tissue culture methods have been experienced for the mass-propagation of this high-valued species. Intact leaves, leaf segments and petiole sections derived from nodal explants in vitro were employed for the optimization of P. odoratissimum micropropagation. The treatment combinations used were MS and 1/2 MS media supplemented with 6-benzylaminopurine, BAP (1, 1.5, 2 and 4.5 mg.L-1) and 1-naphthaleneacetic acid, NAA (0.1, 1 and 1.5 mg.L-1). With leaf segments, the lowest browning incidence, the greatest callogenesis and the highest number of shoots were obtained with the media containing 1.5 mg.L-1 BAP and 1 mg.L-1 NAA. Two mg.L-1 BAP + 0.1 mg.L-1 NAA kept the same results for petiole explants. Intact leaves showed the best results for the three mentioned treatments with 1 mg.L-1 BAP + 1 mg.L-1 NAA. 0.2 mg.L-1 NAA caused the highest rooting percentage and the greatest mean data for the number and length of the roots. Rooted plantlets were transferred to the pots containing 1:1 peat-moss and perlite. Acclimatization of the plantlets was followed by 90 % of survival rate in the greenhouse. The protocol employed would be a potent one to present for the extension section.

Download Full-text

In Vitro Propagation, Genetic Assessment, and Medium-Term Conservation of the Coastal Endangered Species Tetraclinis Articulata (Vahl) Masters (Cupressaceae) from Adult Trees

Plants ◽

10.3390/plants11020187 ◽

2022 ◽

Vol 11 (2) ◽

pp. 187

Author(s):

Jorge Juan-Vicedo ◽

Francisco Serrano-Martínez ◽

Miriam Cano-Castillo ◽

José Luis Casas

Keyword(s):

Basal Medium ◽

Ex Situ ◽

Conservation Strategies ◽

Peat Moss ◽

Naphthaleneacetic Acid ◽

Arid Environments ◽

Multiplication Rate ◽

Tetraclinis Articulata ◽

Adult Trees

Tetraclinis articulata (Vahl) Masters is an endangered tree growing in coastal and arid environments that is widely exploited by the timber and resin industry, among other applications. In this context, the use of in vitro techniques is highly encouraged for its propagation. We present a protocol for micropropagation using twigs from adult trees as a source of explants. The Schenk and Hildebrandt basal medium (SH) supplemented with 30 g L−1 sucrose, 6.5 g L−1 plant agar, 4.0 mg L−1 6-benzyladenine (BA), and 0.05 mg L−1 1-naphthaleneacetic acid (NAA) provided the optimum multiplication rate (90.48 ± 9.52 explants with basal shoots and 2.58 ± 0.29 basal shoots per explant). Application of activated charcoal (AC) or ½ Knop solution in a liquid overlay produced significantly longer shoots. Supplementation of solid media with indole-3-butyric acid (IBA) or NAA gave low rooting percentages (<17%). Addition of 0.9 g L−1 AC improved rooting (40%) but rooting performance was optimal (66.7%) after a pulse treatment consisting of 4 h immersion in liquid SH medium without growth regulators, followed by 8 weeks of cultivation. Rooted microplants were successfully acclimatized (93.33%) in a peat moss and vermiculite mixture (1:1 v/v ratio). The genetic stability of the in vitro regenerated plantlets was confirmed using the randomly amplified polymorphic DNA (RAPD) technique. Explant survival and growth remained higher than 90% after 28 weeks of cold storage at both 4 °C and 10 °C. The protocol presented here allows for largescale T. articulata production and could be applied for both ex situ conservation strategies and industrial purposes.

Download Full-text

Winter Stem Cutting Propagation of ‘Dwarf Burford’ Holly without Use of a Conventional Auxin Treatment

HortTechnology ◽

10.21273/hortsci.19.1.130 ◽

2009 ◽

Vol 19 (1) ◽

pp. 130-132 ◽

Cited By ~ 1

Author(s):

Eugene K. Blythe ◽

Jeff L. Sibley

Keyword(s):

Root Length ◽

Stem Cutting ◽

Naphthaleneacetic Acid ◽

Stem Cuttings ◽

Total Root Length ◽

Growth Responses ◽

Cutting Propagation ◽

Auxin Treatment ◽

Primary Roots ◽

High Humidity Environment

‘Dwarf Burford’ holly (Ilex cornuta ‘Dwarf Burford’) is a significant nursery crop and is widely used in landscapes in U.S. Department of Agriculture hardiness zones 7 to 9. Stem cuttings can be rooted at multiple times during the year, provided cutting wood is sufficiently mature, with auxin treatments traditionally used to encourage rooting. This study was conducted to determine if auxin treatment could be eliminated, thus reducing labor and chemical requirements in the cutting propagation process. In three experiments, terminal stem cuttings of ‘Dwarf Burford’ holly were taken in winter, prepared with and without use of a basal quick-dip in an auxin solution, and rooted in a warm, high-humidity environment. Rooting percentages for nontreated cuttings and cuttings treated with 2500 ppm indole-3-butyric acid (IBA) + 1250 ppm 1-naphthaleneacetic acid (NAA) were similar, while treatment of cuttings with 5000 ppm IBA + 2500 ppm NAA resulted in a decrease in rooting percentage. The number of primary roots and total root length were similar among the three treatments, except in one experiment where total root length was greater with auxin-treated cuttings than with nontreated cuttings. Initial shoot growth responses were variable among the three experiments. The treatment of cuttings with auxin was not required for successful rooting and can be eliminated from the process for winter stem cutting propagation of ‘Dwarf Burford’ holly.

Download Full-text

A fast and effective protocol for obtaining genetically diverse stevia (Stevia rebaudiana Bertoni) regenerants through indirect organogenesis

Agronomy Science ◽

10.24326/as.2021.4.4 ◽

2021 ◽

Vol 76 (4) ◽

pp. 47-62

Author(s):

Magdalena Dyduch-Siemińska

Keyword(s):

Callus Tissue ◽

Phenotypic Variability ◽

Leaf Explants ◽

Stevia Rebaudiana ◽

Nodal Segments ◽

Naphthaleneacetic Acid ◽

Indirect Organogenesis ◽

Stevia Rebaudiana Bertoni ◽

One Step ◽

The Mean

Plant regeneration through indirect organogenesis allows obtaining genetic variability that can be used in the creation of new cultivars. The study presents a fast and effective protocol of one-step preparation of stevia (Stevia rebaudiana Bertoni) regenerants. To obtain callus tissue and shoot regeneration, leaves and nodal segments were used as primary explants, which were placed on MS (Murashige and Skoog) medium supplemented with plant growth regulators (PGRs): NAA (1-naphthaleneacetic acid – 2.0 mg·dm–3, BA (6-benzylaminopurine – 4.0 mg·dm–3), 2,4‑D (2,4-dichlorophenoxyacetic – 2.0 mg·dm–3). Callus tissue was formed on both types of explants, however, only those derived from nodal segments were proliferating. An average of 3.92 shoots per explant were obtained from leaf explants on the applied medium after 6 weeks of culture. The analysis of the morphogenetic capacity of the obtained regenerants was carried out on MS medium supplemented with PGRs – kinetin (0.25 mg·dm–3), BA (0.5 mg·dm–3). The evaluation of the mean number of shoots, mean shoot length (cm), and the mean number of nodes per shoot indicated phenotypic variability of regenerants. The use of RAPD (randomly amplified polymorphic DNA) markers confirmed the differences also at the DNA level. The proposed one-step indirect organogenesis regeneration protocol induced somaclonal variation of Stevia rebaudiana Bertoni and the obtained regenerants, after selection, could be used in the breeding of this species.

Download Full-text

Clonal Propagation of Drypetes malabarica (Bedd.) Airy Shaw An Endemic and Endangered Tree of Southern Western Ghats

Indian Journal of Forestry ◽

10.54207/bsmps1000-2015-m72zqa ◽

2015 ◽

Vol 38 (1) ◽

pp. 35-38

Author(s):

P.A. Jose ◽

M. Sumod ◽

Thomas Varghese

Keyword(s):

Large Scale ◽

Management Practices ◽

Clonal Propagation ◽

Natural Populations ◽

Ex Situ ◽

Stem Cuttings ◽

Endangered Tree ◽

Seedling Bank ◽

Long Run ◽

Propagation Methods

The juvenile plants of Drypetes malabarica have resulted 100% rooting success for both stem cuttings and air layering by the application of NAA 3000 and IAA 1000 ppm respectively. The rooted plants were also survived with cent percent success rate. Since the tree is being prone to high degree of fruit predation, seed infestation and overall poor natural regeneration in situ restricts, the soil seed and seedling bank and merit extend clonal propagation of the species. The propagation methods thus developed first time could be adopted for the large scale multiplication, establishing clonal gene banks ex situ which in turn ensures the availability of materials for conservation, evaluation and management practices without affecting natural populations of the species in the long run.

Download Full-text

Effect of Naphthalene Acetic Acid on the Adventitious Rooting in Shoot Cuttings ofAndrographis paniculata(Burm.f.) Wall. ex Nees: An Important Therapeutical Herb

International Journal of Agronomy ◽

10.1155/2016/1617543 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Md. Sanower Hossain ◽

Zannat Urbi

Keyword(s):

Adventitious Rooting ◽

Peat Moss ◽

Naphthalene Acetic Acid ◽

Naphthaleneacetic Acid ◽

Root Induction ◽

Medical Systems ◽

Once Daily ◽

Apical Shoot ◽

Commercial Scale ◽

Rooting Response

Andrographis paniculatais one of the most important therapeutical herbs, widely used in traditional medical systems for the treatment of diverse diseases for thousands of years. This study was carried out to assess the effect of 1-naphthaleneacetic acid (NAA) on adventitious rooting inA. paniculatashoot cuttings. The cuttings were treated with six concentrations of NAA (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mM) by applying soaking method and cuttings without hormone (soaking in distilled water) were considered as control. The cuttings were then inoculated into peat moss in the planting tray and incubated under complete shade for root induction. Water was sprayed on peat moss once daily to moisten it. The results showed that different concentrations of NAA significantly (P≤0.05) affected the rooting characteristics ofA. paniculataand 2.5 mM of NAA was found to be more effective to induce rooting in young apical shoot (YAS) cuttings compared to other concentrations and old apical shoot (OAS). This study also postulates that adventitious rooting response depends on the juvenility of plant material and concentration of growth regulator. This report describes a technique for adventitious rooting inA.paniculata, which could be feasible to use for commercial scale propagation of this plant.

Download Full-text

In vitro shoot regeneration of boldo from leaf explants

Ciência Rural ◽

10.1590/s0103-84782010005000173 ◽

2010 ◽

Vol 40 (10) ◽

pp. 2210-2213

Author(s):

Monalize Salete Mota ◽

Juliana de Magalhães Bandeira ◽

Eugenia Jacira Bolacel Braga ◽

Valmor João Bianchi ◽

José Antonio Peters

Keyword(s):

Shoot Regeneration ◽

Leaf Explants ◽

Shoot Development ◽

High Potential ◽

Naphthaleneacetic Acid ◽

Regeneration System ◽

Bud Induction ◽

Molecular Studies ◽

In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

Download Full-text

Influence of Plant Growth Regulators and Medium Strength on Micropropagation of the Biodiesel Plant, Jatropha curcus

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v26i1.29770 ◽

2016 ◽

Vol 26 (1) ◽

pp. 85-96 ◽

Cited By ~ 1

Author(s):

Mohamed R Rady ◽

El Sayed ◽

M Mervat ◽

Mohamed El Sayed ◽

Usama I Aly

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Jatropha Curcas ◽

Leaf Explants ◽

Multiple Shoots ◽

Peat Moss ◽

Medium Strength ◽

Half Strength

Leaf explants were cultured to evaluate the effect of different auxins and cytokinins and its concentrations; MS salts on micropropagation of the promising biodiesel Jatropha curcas plant under Egyptian conditions. Results showed that shoot initiated on 0.5 mg/l BA + 0.25 mg/l IBA. Multiplication and elongation were found to be the best using 0.5 mg/l BA in combination with 0.05 mg/l IBA. The multiple shoots were cultured on MS or half strength of MS supplemented with different concentrations of IAA and IBA for rooting phase. Half strength of MS containing 1.0 mg/l IAA was the best for rooting of micropropagated shoots. The rooted plantlets were acclimated in sand : peat?moss mixture (1 : 1) successfully.Plant Tissue Cult. & Biotech. 26(1): 85-96, 2016 (June)

Download Full-text