Obtaining extracts rich in antioxidant polysaccharides from the edible mushroom Pleurotus ostreatus using binary system with hot water and supercritical CO2

2020 ◽  
Vol 330 ◽  
pp. 127173 ◽  
Author(s):  
Jhonatas Rodrigues Barbosa ◽  
Maurício Madson S. Freitas ◽  
Luã Caldas Oliveira ◽  
Luiza Helena S. Martins ◽  
Andryo Orfi Almada-Vilhena ◽  
...  
Keyword(s):  
Binary System ◽  
Pleurotus Ostreatus ◽  
Supercritical Co2 ◽  
Edible Mushroom ◽  
Hot Water

A heteropolysaccharide from aqueous extract of an edible mushroom, Pleurotus ostreatus cultivar: structural and biological studies

2011 ◽  
Vol 346 (2) ◽  
pp. 366-372 ◽  
Author(s):  
Kankan K. Maity ◽  
Sukesh Patra ◽  
Biswajit Dey ◽  
Sanjoy K. Bhunia ◽  
Soumitra Mandal ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Pleurotus Ostreatus ◽  
Edible Mushroom ◽  
Biological Studies

Growth and yield impact of oyster mushroom Pleurotus ostreatus (Jacq P. Kumm) cultivated on different agricultural wastes

2021 ◽  
Vol 27 (01) ◽  
pp. 2225-2233
Author(s):  
M. O. Agba ◽  
◽  
A. A. Markson ◽  
J. O. Oni ◽  
G. A. Bassey ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Growth Parameters ◽  
Total Yield ◽  
Fruit Body ◽  
Food Product ◽  
Dry Weight ◽  
Edible Mushroom ◽  
Growth And Yield ◽  
Biological Efficiency ◽  
Essential Food

Pleurotus ostreatus, an edible mushroom is an essential food product. Recognised as one of the cheapest sources of proteins, their high nutritional and anti-nutritional properties are of immense importance to humans. The present study evaluated the effect of sawdust and dried plantain leaves on the growth and yield of Pleurotus ostreatus. Sawdust and dried plantain leaves were composted into seven substrates; as single substrates and at different combinations. The composted substrates include sawdust 100 % (M0), Dry plantain leaves 100% (M1), Dry plantain leaves + sawdust at the ratio of 1:4 (M2), 2:3 (M3), 3:2 (M4), 1:1 (M5) and 4:1(M6). Growth parameters of interest assessed flush include mycelia running rate (MRR), area of pileus, length of the stipe, the girth of the stipe, fresh weight and dry weight, number of fruit body, total yield and biological efficiency. At the end of the three weeks spawn running period, M0 was overall best in supporting mycelia running rate with a mean MRR of 16.00 cm. M1 substrates produced mushroom with longer and bigger stipes (7.17 cm) at the first flush. A higher number of fruit bodies (82.66), total yield (130.35 g), and biological efficiency (43.45 g) were all seen in M0 substrates. Sawdust at 100 % (M0) proved to be the best substrate for the cultivation of Pleurotus ostreatus.

scholarly journals Effect of Different substrate Disinfection Methods on the Production of Pleurotus ostreatus

2016 ◽  
Vol 4 (4) ◽  
pp. 52 ◽  
Author(s):  
Funda Atila
Keyword(s):  
Rural Areas ◽  
Pleurotus Ostreatus ◽  
Hot Water ◽  
Biological Efficiency ◽  
Disinfection Methods ◽  
Chemical Disinfection

AbstractThe aim of this paper is to estimate which of disinfection method is more effective in case of higher yield and fewer cost in cultivation of Pleurotus ostreatus in rural areas.  For this purpose, four different treatments; scalping in hot water (60, 80 and 100°C), and chemical disinfection (1% formaldehyde) were compared with sterilization by autoclave. It has been noticed that method of disinfection affects mycelium growing duration(day), yield(g/kg), biological efficiency (BE%), avarage weight of fruitbody(g), diameter of pileus(cm), stipe lenght(cm) and width(cm).  P. ostreatus took significantly much time to colonise the substrate (19.9 days) disinfected by chemical disinfection compared with others. Chemical disinfection (271.28 g/kg and 91.96%) and scalding hot water at 80oC (263.42 g/kg and 90.83%) had highest productivity in terms of yield and BE, respectively, while scalding hot water at 60oC (136.08 g/kg and 46.92%) had least productivity. The largest diameter of fruitbody was obtained by scalding in hot water at 80oC, while the longest stipes were harvested in disinfected substrate by chemicals.  In conclusion, cheaper and more easily applecable scalping in hot water 80°C and chemical disinfection may be use for the disinfection of growing substrates instead of sterilization with autoclave in rural areas.Keywords: mushroom growing substrate, disinfection methods, autoclaved, scalding, chemical disinfection, Pleurotus ostreatus

scholarly journals Biological Properties of Silver Nanoparticles from β (1-3) Glucan Isolated from the Edible Mushroom Pleurotus florida

2020 ◽  
Vol 10 (2) ◽  
pp. 2096-2106
Keyword(s):  
Silver Nanoparticles ◽  
Drug Development ◽  
Hydroxyl Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Edible Mushroom ◽  
Hot Water ◽  
Dependent Manner ◽  
Pleurotus Florida ◽  
Radical Scavenging Assay

Pleurotus florida of the genus Pleurotus is a delicious edible mushroom with high therapeutic potential. Silver nanoparticles (AgNPs) are gaining a lot of importance nowadays because of their wide industrial and biomedical applications. Capping of AgNPs with polysaccharides, the most potent mushroom derived metabolites, is promising towards natural drug development for free radical-induced ailments. In the present investigation, silver nanoparticles were synthesized using β-glucan isolated from P. florida, and their antioxidant properties were studied. Polysaccharide (PS) isolation was carried out by hot water extraction, alcohol precipitation, deproteinization, and dialysis. Preliminary characterization of PS by Anthrone, Lowry’s method, Paper Chromatography, and FT-IR showed that PS is protein-bound β-(1-3) - Glucan. Synthesis of Silver nanoparticle from PS (PS-AgNPs) was confirmed by UV spectroscopy. Antioxidant properties of PS and PS-AgNPs were evaluated by Total antioxidant capacity assay, DPPH assay, Total reducing power, and Hydroxyl radical scavenging assay. Both PS and PS-AgNPs showed profound antioxidant activity in a dose-dependent manner, and PS-AgNPs was more active than PS. The highest activity was shown in Hydroxyl radical scavenging assay in which PS and PS-AgNPs showed 81.8% and 89.4% activity, respectively, at the highest tested dose. The present study revealed the possible use of P. florida in antioxidant drug development.

scholarly journals Antinociceptive effect of Pleurotus ostreatus (Oyster Mushroom) Aqueous Extracts in Rattus norvegicus (Albino Rats)

1970 ◽  
Vol 20 (2) ◽  
pp. 483-487
Author(s):  
LF Zitte ◽  
RS Konya
Keyword(s):  
Aqueous Extract ◽  
Rattus Norvegicus ◽  
Pleurotus Ostreatus ◽  
Hot Water ◽  
Oyster Mushroom ◽  
Albino Rats ◽  
Thermal Stimulus ◽  
Aqueous Extracts ◽  
Standard Drug ◽  
Nociceptive Responses

Antinociceptive is reducing sensitivity to painful stimuli for the individual. The objective of this study was to evaluate the antinociceptive potentials of graded doses of Pleurotus ostreatus (Oyster Mushroom) aqueous extract in Rattus norvegicus (Albino Rats) and its chemical pattern by comparing it with a standard drug and a control using the hot water based flick tail test. Thirty five adult rats of both sexes were used for the experiment which, were divided into five groups of seven rats per group. Group one was used as the control (with 1ml normal saline), while groups two, three and four were treated with 100, 200 and 400 mg/kg of Pleurotus ostreatus aqueous extracts and group five treated with 15 mg/kg Aspirin (a standard drug) as positive control. Hot water at 550C was used to determine the nociceptive responses of the animals to detect anti-nociceptive effects of Pleurotus ostreatus extracts as compared to the control in hot water inflicted pain. The results suggested that Pleurotus ostreatus aqueous extract exhibits antinociceptive properties against thermal stimulus at 550C. The diversity of individual animals’ pain tolerance threshold when immersed in hot water was also observed during the experiment. However, the extract indicated a high degree of anti-nociceptive effect at 30, 60, 90 and 120 minutes post treatment period, with a progressively longer threshold time for pain sensitivity. It was also found that at 90 minute period, the control portrayed a relatively short response time.KEY WORDS: Anti-nociceptive, threshold pain, Pleurotus ostreatus, hot water test flick tail.

scholarly journals Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2740 ◽  
Author(s):  
Ekaterina Baeva ◽  
Roman Bleha ◽  
Ekaterina Lavrova ◽  
Leonid Sushytskyi ◽  
Jana Čopíková ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Pleurotus Ostreatus ◽  
Cold Water ◽  
Water Soluble ◽  
Hot Water ◽  
Biologically Active ◽  
Alkaline Extract ◽  
A Cell ◽  
Oyster Mushrooms

Oyster mushrooms are an interesting source of biologically active glucans and other polysaccharides. This work is devoted to the isolation and structural characterization of polysaccharides from basidiocarps of the cultivated oyster mushroom, Pleurotus ostreatus. Five polysaccharidic fractions were obtained by subsequent extraction with cold water, hot water and two subsequent extractions with 1 m sodium hydroxide. Branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan predominated in cold- and hot-water-soluble fractions, respectively. Alternatively, these polysaccharides were obtained by only hot water extraction and subsequent two-stage chromatographic separation. The alkali-soluble parts originating from the first alkali extraction were then fractionated by dissolution in dimethyl sulfoxide (DMSO). The polysaccharide insoluble in DMSO was identified as linear (1→3)-α-d-glucan, while branched (1→3)(1→6)-β-d-glucans were found to be soluble in DMSO. The second alkaline extract contained the mentioned branched β-d-glucan together with some proteins. Finally, the alkali insoluble part was a cell wall complex of chitin and β-d-glucans.

scholarly journals Comparative Study of Pleurotus ostreatus Mushroom Grown on Modified PAN Nanofiber Mats

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 475 ◽  
Author(s):  
Lilia Sabantina ◽  
Franziska Kinzel ◽  
Thomas Hauser ◽  
Astrid Többer ◽  
Michaela Klöcker ◽  
...  
Keyword(s):  
Comparative Study ◽  
Growth Rates ◽  
Pleurotus Ostreatus ◽  
Edible Mushroom ◽  
Fungal Mycelium ◽  
Specific Substrate ◽  
Biotechnological Applications ◽  
Mushroom Species ◽  
Nanofiber Mats ◽  
Pan Nanofiber

Pleurotus ostreatus is a well-known edible mushroom species which shows fast growth. The fungus can be used for medical, nutritional, filter, or packaging purposes. In this study, cultivation experiments were carried out with Pleurotus ostreatus growing on polyacrylonitrile (PAN) nanofiber mats in the presence of saccharose and Lutrol F68. The aim of this study was to find out whether modified PAN nanofiber mats are well suited for the growth of fungal mycelium, to increase growth rates and to affect mycelium fiber morphologies. Our results show that Pleurotus ostreatus mycelium grows on nanofiber mats in different morphologies, depending on the specific substrate, and can be used to produce a composite from fungal mycelium and nanofiber mats for biomedical and biotechnological applications.

scholarly journals Antioxidant Capacity and the Correlation with Major Phenolic Compounds, Anthocyanin, and Tocopherol Content in Various Extracts from the Wild EdibleBoletus edulisMushroom

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Emanuel Vamanu ◽  
Sultana Nita
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Edible Mushroom ◽  
Tocopherol Content ◽  
Hot Water ◽  
Antioxidant Compounds ◽  
Methanolic Extracts ◽  
Freeze Dried

Boletus edulisis a wild edible mushroom habitually consumed by rural populations. Ethanolic and methanolic extracts was obtained in cold and hot water from dried fruit bodies. The antioxidant activity of freeze-dried extracts fromB. eduliswere investigated using free radicals scavenging activity, reducing power, metal chelating effect, inhibition of lipid peroxidation, and the identification of antioxidant compounds. The levels of different compounds with antioxidant properties were higher in alcoholic extracts compared with aqueous extracts. Rosmarinic acid was the major phenolic compound, it being identified in a concentration between7±0.23and56±0.15 mg/100 g extract. A positive correlation between the content of total phenols, flavonoids, anthocyanins, and tocopherols, and the antioxidant capacity of the extracts was determined. The results showed that the ethanolic extract of Romanian wild mushroomB. edulisrepresents a natural source of functional compounds.

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