A heteropolysaccharide from aqueous extract of an edible mushroom, Pleurotus ostreatus cultivar: structural and biological studies

Edible mushrooms aside being taken as foods, are used in ethnomedicine in the management of various ailments notably tumours and related ailments whose pathophysiology are linked to oxidative stress. This study investigated the anti-mitotic, and antibacterial activities of the aqueous extract of the edible mushroom Pleurotus ostreatus based on ethno-medicine.The Allium cepa anti-mitotic assay model was used for anti-proliferative investigation of the defatted aqueous ethanol extract at concentration range of: 10.00 – 0.08 ng/mL following a two-fold serial dilution approach. Methotrexate (0.25 ng/mL) and portable water were used as reference standard for positive control and negative control respectively. The student t-test was used for statistical analysis (p < 0.05). Antibacterial susceptibility evaluation against clinical isolates of selected pathogenic organisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aereus, and Bacillus subtilis was done using the Agar dilution method at 1000 and 10 µg/mL. The aqueous extract showed a dose and time dependent anti-mitotic activity with the three higher doses: 10.00, 5.00 and 2.50 ng/mL exhibiting complete inhibition of mitosis which was comparable to the reference drug methotrexate (0.25 ng/mL) after 96 hours incubation period. Although the aqueous extract was not bacteriocidal at the test concentration, a dose dependent bacteriostatic effect against E. coli, and B. subtilis was observed. The observed anti-mitotic activity of this mushroom validates its ethnomedicinal use in the treatment of tumours and related diseases.

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Growth and yield impact of oyster mushroom Pleurotus ostreatus (Jacq P. Kumm) cultivated on different agricultural wastes

Journal of Bioscience and Agriculture Research ◽

10.18801/jbar.270121.272 ◽

2021 ◽

Vol 27 (01) ◽

pp. 2225-2233

Author(s):

M. O. Agba ◽

◽

A. A. Markson ◽

J. O. Oni ◽

G. A. Bassey ◽

...

Keyword(s):

Pleurotus Ostreatus ◽

Growth Parameters ◽

Total Yield ◽

Fruit Body ◽

Food Product ◽

Dry Weight ◽

Edible Mushroom ◽

Growth And Yield ◽

Biological Efficiency ◽

Essential Food

Pleurotus ostreatus, an edible mushroom is an essential food product. Recognised as one of the cheapest sources of proteins, their high nutritional and anti-nutritional properties are of immense importance to humans. The present study evaluated the effect of sawdust and dried plantain leaves on the growth and yield of Pleurotus ostreatus. Sawdust and dried plantain leaves were composted into seven substrates; as single substrates and at different combinations. The composted substrates include sawdust 100 % (M0), Dry plantain leaves 100% (M1), Dry plantain leaves + sawdust at the ratio of 1:4 (M2), 2:3 (M3), 3:2 (M4), 1:1 (M5) and 4:1(M6). Growth parameters of interest assessed flush include mycelia running rate (MRR), area of pileus, length of the stipe, the girth of the stipe, fresh weight and dry weight, number of fruit body, total yield and biological efficiency. At the end of the three weeks spawn running period, M0 was overall best in supporting mycelia running rate with a mean MRR of 16.00 cm. M1 substrates produced mushroom with longer and bigger stipes (7.17 cm) at the first flush. A higher number of fruit bodies (82.66), total yield (130.35 g), and biological efficiency (43.45 g) were all seen in M0 substrates. Sawdust at 100 % (M0) proved to be the best substrate for the cultivation of Pleurotus ostreatus.

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Antinociceptive effect of Pleurotus ostreatus (Oyster Mushroom) Aqueous Extracts in Rattus norvegicus (Albino Rats)

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v20i2.31 ◽

1970 ◽

Vol 20 (2) ◽

pp. 483-487

Author(s):

LF Zitte ◽

RS Konya

Keyword(s):

Aqueous Extract ◽

Rattus Norvegicus ◽

Pleurotus Ostreatus ◽

Hot Water ◽

Oyster Mushroom ◽

Albino Rats ◽

Thermal Stimulus ◽

Aqueous Extracts ◽

Standard Drug ◽

Nociceptive Responses

Antinociceptive is reducing sensitivity to painful stimuli for the individual. The objective of this study was to evaluate the antinociceptive potentials of graded doses of Pleurotus ostreatus (Oyster Mushroom) aqueous extract in Rattus norvegicus (Albino Rats) and its chemical pattern by comparing it with a standard drug and a control using the hot water based flick tail test. Thirty five adult rats of both sexes were used for the experiment which, were divided into five groups of seven rats per group. Group one was used as the control (with 1ml normal saline), while groups two, three and four were treated with 100, 200 and 400 mg/kg of Pleurotus ostreatus aqueous extracts and group five treated with 15 mg/kg Aspirin (a standard drug) as positive control. Hot water at 550C was used to determine the nociceptive responses of the animals to detect anti-nociceptive effects of Pleurotus ostreatus extracts as compared to the control in hot water inflicted pain. The results suggested that Pleurotus ostreatus aqueous extract exhibits antinociceptive properties against thermal stimulus at 550C. The diversity of individual animals’ pain tolerance threshold when immersed in hot water was also observed during the experiment. However, the extract indicated a high degree of anti-nociceptive effect at 30, 60, 90 and 120 minutes post treatment period, with a progressively longer threshold time for pain sensitivity. It was also found that at 90 minute period, the control portrayed a relatively short response time.KEY WORDS: Anti-nociceptive, threshold pain, Pleurotus ostreatus, hot water test flick tail.

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Enhancement of The Edible Mushroom Pleurotus Ostreatus Yield via A New Liquid Fermentation Tank

Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016) ◽

10.2991/bep-16.2017.11 ◽

2017 ◽

Author(s):

Xiaoyu DUAN ◽

Yu HU ◽

Yuxian YOU ◽

Qianqian TANG ◽

Yiwen LI ◽

...

Keyword(s):

Pleurotus Ostreatus ◽

Edible Mushroom ◽

Liquid Fermentation ◽

Fermentation Tank

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Comparative Study of Pleurotus ostreatus Mushroom Grown on Modified PAN Nanofiber Mats

Nanomaterials ◽

10.3390/nano9030475 ◽

2019 ◽

Vol 9 (3) ◽

pp. 475 ◽

Cited By ~ 9

Author(s):

Lilia Sabantina ◽

Franziska Kinzel ◽

Thomas Hauser ◽

Astrid Többer ◽

Michaela Klöcker ◽

...

Keyword(s):

Comparative Study ◽

Growth Rates ◽

Pleurotus Ostreatus ◽

Edible Mushroom ◽

Fungal Mycelium ◽

Specific Substrate ◽

Biotechnological Applications ◽

Mushroom Species ◽

Nanofiber Mats ◽

Pan Nanofiber

Pleurotus ostreatus is a well-known edible mushroom species which shows fast growth. The fungus can be used for medical, nutritional, filter, or packaging purposes. In this study, cultivation experiments were carried out with Pleurotus ostreatus growing on polyacrylonitrile (PAN) nanofiber mats in the presence of saccharose and Lutrol F68. The aim of this study was to find out whether modified PAN nanofiber mats are well suited for the growth of fungal mycelium, to increase growth rates and to affect mycelium fiber morphologies. Our results show that Pleurotus ostreatus mycelium grows on nanofiber mats in different morphologies, depending on the specific substrate, and can be used to produce a composite from fungal mycelium and nanofiber mats for biomedical and biotechnological applications.

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