Growth and Lipid Production by Desmodesmus subspicatus and Potential of Lipids for Biodiesel Production

2017 ◽  
Vol 4 ◽  
pp. 58-63
Author(s):  
Rajni Chaudhary ◽  
J.I.S Khattar ◽  
D.P Singh
Keyword(s):  
Lipid Production ◽  
Biodiesel Production ◽  
Desmodesmus Subspicatus

Optimization of lipid accumulation in Pleurastrum insigne for biodiesel production

2021 ◽  
Vol 16 (10) ◽  
pp. 144-155
Author(s):  
Van Lal Michael Chhandama ◽  
Belur Kumudini Satyan
Keyword(s):  
Lipid Content ◽  
Lipid Production ◽  
Statistical Design ◽  
High Growth ◽  
High Growth Rate ◽  
Biodiesel Production ◽  
Nitrogen And Phosphorus ◽  
Biodiesel Standards ◽  
High Lipid ◽  
Different Sources

Microalgae emerged as a competent feedstock for biodiesel production because of high growth rate and lipid content. This work focuses on isolation of novel microalgal strain from different sources of water for the production of biodiesel. The isolated microalgae, Pleurastrum insigne possessed high lipid content (~28 % dcw), further optimized to 57.06 % dcw using a statistical design (CCD) under Response Surface Methodology. Lipid production was optimized by nutrient (nitrogen and phosphorus) and pH stress. The different type of fatty acids present in the optimized lipid was also profiled using GCMS. Biodiesel yield was found to be 82.14 % of the total lipid and the fuel properties tested have met IS, ASTM and EN biodiesel standards.

scholarly journals Statistical Approach of Nutrient Optimization for Microalgae Cultivation

2020 ◽  
Vol 141 ◽  
pp. 03009
Author(s):  
Pichayatorn Bunkaew ◽  
Sasithorn Kongruang
Keyword(s):  
Light Intensity ◽  
Cobalt Chloride ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Microalgae Cultivation ◽  
Cultivation Time ◽  
Chlorella Sp ◽  
Chloride Hexahydrate ◽  
Cobalt Chloride Hexahydrate

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

scholarly journals Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Francesca Martani ◽  
Letizia Maestroni ◽  
Mattia Torchio ◽  
Diletta Ami ◽  
Antonino Natalello ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Lipid Content ◽  
Lipid Production ◽  
Batch Process ◽  
Batch Cultivation ◽  
Biodiesel Production ◽  
Cellular Lipid ◽  
Lipomyces Starkeyi ◽  
Yeast Cultures ◽  
Beet Pulp

Abstract Background Lipids from oleaginous yeasts emerged as a sustainable alternative to vegetable oils and animal fat to produce biodiesel, the biodegradable and environmentally friendly counterpart of petro-diesel fuel. To develop economically viable microbial processes, the use of residual feedstocks as growth and production substrates is required. Results In this work we investigated sugar beet pulp (SBP) and molasses, the main residues of sugar beet processing, as sustainable substrates for the growth and lipid accumulation by the oleaginous yeast Lipomyces starkeyi. We observed that in hydrolysed SBP the yeast cultures reached a limited biomass, cellular lipid content, lipid production and yield (2.5 g/L, 19.2%, 0.5 g/L and 0.08 g/g, respectively). To increase the initial sugar availability, cells were grown in SBP blended with molasses. Under batch cultivation, the cellular lipid content was more than doubled (47.2%) in the presence of 6% molasses. Under pulsed-feeding cultivation, final biomass, cellular lipid content, lipid production and lipid yield were further improved, reaching respectively 20.5 g/L, 49.2%, 9.7 g/L and 0.178 g/g. Finally, we observed that SBP can be used instead of ammonium sulphate to fulfil yeasts nitrogen requirement in molasses-based media for microbial oil production. Conclusions This study demonstrates for the first time that SBP and molasses can be blended to create a feedstock for the sustainable production of lipids by L. starkeyi. The data obtained pave the way to further improve lipid production by designing a fed-batch process in bioreactor. Graphical abstract

scholarly journals Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

Energies ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3648
Author(s):  
Savienne M. F. E. Zorn ◽  
Cristiano E. R. Reis ◽  
Messias B. Silva ◽  
Bo Hu ◽  
Heizir F. De Castro
Keyword(s):  
Lipid Accumulation ◽  
Unsaturated Fatty Acids ◽  
Lipid Production ◽  
Synthetic Medium ◽  
Fungal Spores ◽  
Dry Weight ◽  
Biodiesel Production ◽  
Biomass Composition ◽  
Fungal Mycelium ◽  
Fourfold Increase

This study aims to evaluate the potential of consortium biomass formation between Mucor circinelloides, an oleaginous filamentous fungal species, and Chlorella vulgaris, in order to promote a straightforward approach to harvest microalgal cells and to evaluate the lipid production in the consortium system. A synthetic medium with glucose (2 g·L−1) and mineral nutrients essential for both fungi and algae was selected. Four different inoculation strategies were assessed, considering the effect of simultaneous vs. separate development of fungal spores and algae cells, and the presence of a supporting matrix aiming at the higher recovery of algae cell rates. The results were evaluated in terms of consortium biomass composition, demonstrating that the strategy using a mature fungal mycelium with a higher algae count may provide biomass samples with up to 79% of their dry weight as algae, still promoting recovery rates greater than 97%. The findings demonstrate a synergistic effect on the lipid accumulation by the fungal strain, at around a fourfold increase when compared to the axenic control, with values in the range of 23% of dry biomass weight. Furthermore, the fatty acid profile from the samples presents a balance between saturated and unsaturated fatty acids that is likely to present an adequate balance for applications such as biodiesel production.

scholarly journals Exogenous l-proline improved Rhodosporidium toruloides lipid production on crude glycerol

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Rasool Kamal ◽  
Yuxue Liu ◽  
Qiang Li ◽  
Qitian Huang ◽  
Qian Wang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Lipid Content ◽  
Crude Glycerol ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Rhodosporidium Toruloides ◽  
Biodiesel Production ◽  
Lipid Yield ◽  
Stress Agent ◽  
Glycerol Consumption

Abstract Background Crude glycerol as a promising feedstock for microbial lipid production contains several impurities that make it toxic stress inducer at high amount. Under stress conditions, microorganisms can accumulate l-proline as a safeguard. Herein, l-proline was assessed as an anti-stress agent in crude glycerol media. Results Crude glycerol was converted to microbial lipids by the oleaginous yeast Rhodosporidium toruloides CGMCC 2.1389 in a two-staged culture mode. The media was supplied with exogenous l-proline to improve lipid production efficiency in high crude glycerol stress. An optimal amount of 0.5 g/L l-proline increased lipid titer and lipid yield by 34% and 28%, respectively. The lipid titer of 12.2 g/L and lipid content of 64.5% with a highest lipid yield of 0.26 g/g were achieved with l-proline addition, which were far higher than those of the control, i.e., lipid titer of 9.1 g/L, lipid content of 58% and lipid yield of 0.21 g/g. Similarly, l-proline also improved cell growth and glycerol consumption. Moreover, fatty acid compositional profiles of the lipid products was found suitable as a potential feedstock for biodiesel production. Conclusion Our study suggested that exogenous l-proline improved cell growth and lipid production on crude glycerol by R. toruloides. The fact that higher lipid yield as well as glycerol consumption indicated that l-proline might act as a potential anti-stress agent for the oleaginous yeast strain.

Application of p-coumaric acid for extraordinary lipid production in Tetradesmus obliquus: A sustainable approach towards enhanced biodiesel production

2020 ◽  
Vol 157 ◽  
pp. 368-376 ◽  
Author(s):  
Sivakumar Esakkimuthu ◽  
Venkatesan Krishnamurthy ◽  
Shuang Wang ◽  
Xun Hu ◽  
Swaminathan K ◽  
...  
Keyword(s):  
Lipid Production ◽  
Biodiesel Production ◽  
Coumaric Acid ◽  
Tetradesmus Obliquus

scholarly journals Genetic engineering of microalgae lipid biosynthesis for sustainable biodiesel production

2021 ◽  
Vol 11 (3) ◽  
pp. 072-077
Author(s):  
Siti Zulaiha
Keyword(s):  
Genetic Engineering ◽  
Metabolic Pathways ◽  
Alternative Energy ◽  
Fossil Fuels ◽  
Lipid Production ◽  
High Growth ◽  
Lipid Biosynthesis ◽  
Production Costs ◽  
Biodiesel Production ◽  
Promising Alternative

Biofuel is one of the most promising alternative energy sources for reducing human reliance on fossil fuels. Microalgae has recently emerged as the most promising biofuel source. However, biofuels from microalgae are still not feasible to replace fossil fuels because of their high production costs, therefore, it is necessary to pick microalgae species with high growth rates and lipid content. Overexpression of lipid biosynthesis enzymes and inhibition of competitive metabolic pathways are two genetic engineering strategies that can be developed to assess microalgae lipid production. Malate and multienzyme enzymes (GPAT, LPAAT and DGAT) can be overexpressed in microalgae to boost lipid production. The strategy of blocking competitive metabolic pathways can be carried out through suppression of starch metabolism and lipid catabolism. The strategy of blocking competitive metabolic pathways has been carried out in several microalgae and is effective for enhancing lipid biosynthesis. Several mutations that block both the starch metabolic and lipid catabolic pathways can result in increased levels of microalgal lipid accumulation.

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