scholarly journals Simultaneous Use of Factor XIII and Fibrin Degradation Products in Diagnosing Early Cases of NEC and Neonatal SEPSIS

2021 ◽  
Vol 2 (4) ◽  
pp. 1-10
Author(s):  
Mohammad Abdelmonaem Sharaf ◽  
Heba Ezzat Hashem ◽  
Wafaa O. Ahmed
Keyword(s):  
Neonatal Sepsis ◽  
Factor Xiii ◽  
Degradation Products ◽  
Laboratory Data ◽  
Sepsis Group ◽  
Laboratory Evaluation ◽  
Preterm Neonates ◽  
Fibrin Degradation Products ◽  
Plasma Factor

Purpose: The study examined the use of factor XIII and fibrin degradation products in diagnosing early cases of NEC and neonatal sepsis. Methods: Sixty neonates were divided into two groups. 30 preterm neonates suspected with early NEC Diagnosis of NEC was confirmed by modified Bell’s score and 30 preterm neonates with symptoms of neonatal sepsis; where sepsis was confirmed by blood culture and CRP. Laboratory evaluation of FDPs and plasma factor XIII was done for all the patients. The study was carried out in a tertiary NICU of the pediatric department, Ain Shams University Hospital. All enrolled neonates had a matched mean birth weight and gestational age. They were either moderate preterms >32 weeks, but <34 weeks, and late preterms >34 weeks, but <37 weeks). Results: The results indicate a correlation between FDPs and the laboratory data of group B, and it was found out that FDPs were negatively correlated with TLC, Plate-lets, and CRP, reflecting FDPs increase with bone marrow suppression and progression of sepsis. Factor XIII was significantly lower in the group with NEC as compared to the group of sepsis (p<0.001), while FDPs level was significantly higher in the group with sepsis (p<. 0.001). The correlation between the clinical stages of NEC BELL's score and the level of Initial factor XIII level revealed that the factor level is negatively correlated with stage I of BELL's score. The follow-up revealed that there was no correlation between BELL's score and the level of follow-up factor XIII. On follow-up, the current study demonstrated that TLC, CRP, FDPS, PTT were significantly increased in the sepsis group with p values of 0.021,, 0.001, 0.001 and 0.01. The current study found significantly higher partial thromboplastin time (PTT) in the group with sepsis Conclusion: Factor XIII level can predict early cases of NEC and can differentiate it from neo-natal sepsis.

scholarly journals cfDNA and DNases: New Biomarkers of Sepsis in Preterm Neonates—A Pilot Study

Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 192
Author(s):  
Moritz Lenz ◽  
Thomas Maiberger ◽  
Lina Armbrust ◽  
Antonia Kiwit ◽  
Axel Von der Wense ◽  
...  
Keyword(s):  
Preterm Infants ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Late Onset ◽  
Dnase I ◽  
Sepsis Group ◽  
Preterm Neonates ◽  
Control Group ◽  
Suspected Sepsis ◽  
Epidemiological Characteristics

Introduction: An early and accurate diagnosis of early onset neonatal sepsis (EONS) and late onset neonatal sepsis (LONS) is essential to improve the outcome of this devastating conditions. Especially, preterm infants are at risk. Reliable biomarkers are rare, clinical decision-making depends on clinical appearance and multiple laboratory findings. Markers of NET formation and NET turnover might improve diagnostic precision. Aim of this study was to evaluate the diagnostic value of NETs in sepsis diagnosis in neonatal preterm infants. Methods: Plasma samples of neonatal preterm infants with suspected sepsis were collected. Blood samples were assayed for markers of NET formation and NET turnover: cfDNA, DNase1, nucleosome, NE, and H3Cit. All clinical findings, values of laboratory markers, and epidemiological characteristics were collected retrospectively. Two subpopulations were created to divide EONS from LONS. EMA sepsis criteria for neonatal sepsis were used to generate a sepsis group (EMA positive) and a control group (EMA negative). Results: A total of 31 preterm neonates with suspected sepsis were included. Out of these, nine patients met the criteria for sepsis according to EMA. Regarding early onset neonatal sepsis (3 EONS vs. 10 controls), cfDNA, DNase I, nucleosome, and CRP were elevated significantly. H3Cit and NE did not show any significant elevations. In the late onset sepsis collective (6 LONS vs. 12 controls), cfDNA, DNase I, and CRP differed significantly compared to control group.

Determination Of The Type Of Fibrinogen/Fibrin Degradation Products In The Preoperative Evaluation Of Pelvic Masses

1981 ◽  
Author(s):  
L Kaplan-Ikonicoff ◽  
A M Engel ◽  
F R Roisman ◽  
A Demattei
Keyword(s):  
Degradation Products ◽  
Preoperative Evaluation ◽  
Fibrin Degradation Products ◽  
Pelvic Masses ◽  
Postoperative Diagnosis ◽  
Potential Benefits ◽  
Single Method ◽  
Age Range

It is known that no single method for the determination of fibrinogen/fibrin degradation products (FDP) indicates their nature which is important to establish the state of activation of the fibrinolytic system. Therefore we applied two simple and reproducible laboratory methods to study eleven preoperative patients with pelvic masses: 1) the latex fibrin polymerization inhibition test (Finkelstein test)and 2) the staphylococcal cumpling test. The normal values obtained were 0-3 polymerization inhibition units (PIU) and 2.5-4 μg fibrinogen equivalents per milliliter, respectively. Seven clinically healthy females of the same age range served as controls.Out of seven cases with confirmed postoperative diagnosis of ovarian carcinoma, five (71%) had positive FDP by both methods with average values of 12.2 ± 5.9 PIU and 10.4 ± 4.6 μg/ml. The ratio between PlU/ml and μg/ml was determined and the range found was 0.53-1.75 which is indicative of the presence of FDP of the “late” type. The values are in agreement with previous reports of the incidence of late FDP in other malignancies. All the negative FDP, except two, corresponded to benign ovarian cyst, although there was a case of haemorrhagic cyst with positive FDP.The presence of “late” FDP indicates an activated fibrinolytic process and could constitute a cautionary sign for the postoperative care of the patient. We consider that the application of two methods for FDP permits to detect an increased amount and also the type of the circulating FDP. The data presented here clearly warrant detailed investigation of the potential benefits of knowing the nature of FDP in monitoring the postoperative follow up of patients with pelvic masses.

Elastase Mediated Fibrinolysis in Acute Promyelocytic Leukemia

2000 ◽  
Vol 83 (06) ◽  
pp. 906-908 ◽  
Author(s):  
Erik-Jan Oudijk ◽  
H. Nieuwenhuis ◽  
Rogier Bos ◽  
Rob Fijnheer
Keyword(s):  
Acute Promyelocytic Leukemia ◽  
Degradation Products ◽  
Promyelocytic Leukemia ◽  
Sepsis Group ◽  
Minor Role ◽  
Hemorrhagic Diathesis ◽  
Elastase Activity ◽  
Fibrin Degradation Products ◽  
A Minor

SummaryThe bleeding syndrome of acute promyelocytic leukemia (APL) is complex and consists of disseminated intravascular coagulation (DIC) and hyperfibrinolysis. Elastase, derived from malignant promyelocytes, is believed to mediate the fibrinogeno- and fibrinolysis by aspecific proteolysis. In this study we measured the role of elastase in fifteen patients with APL by using an assay for elastase degraded fibrin(ogen) and the results were compared with those obtained in patients with sepsis induced DIC.High levels of elastase were observed in sepsis and APL. The levels of fibrinogen and fibrin degradation products were significantly higher in APL patients compared to patients with sepsis induced DIC. Nevertheless, the level of elastase degraded fibrin(ogen) was higher in the sepsis group (635.3 ng/ml, compared to 144.3 ng/ml in APL; p <0.0001). So, the enormous increase in fibrin and fibrinogen degradation products in APL cannot be explained by elastase activity. This study suggests a minor role for elastase mediated proteolysis in the hemorrhagic diathesis in APL patients.

scholarly journals A Case of Bilateral Retinal Hemorrhage with Cotton Wool Spot Following Viper Bite

2015 ◽  
Vol 14 (3) ◽  
pp. 297-298
Author(s):  
Subhasis Jana ◽  
Saumen Kumar Chaudhuri ◽  
Purban Ganguly ◽  
Sumi Ghorai
Keyword(s):  
Degradation Products ◽  
Medical Science ◽  
Retinal Hemorrhage ◽  
Fundus Examination ◽  
Cotton Wool ◽  
Retinal Hemorrhages ◽  
Cotton Wool Spots ◽  
Fibrin Degradation Products ◽  
History Of

A 38 years male, admitted in the department of Internal Medicine with a history of snake bite in his left lower leg during agriculture work. He was treated with anti snake venom after admission. He had history of dimness of vision in both eyes. On examination, swelling and subcutaneous hemorrhage was noted in his left leg. His best corrected visual acuity (BCVA) was 6/60 in the both eyes. Fundus examination with 90 D lens and Indirect Ophthalmoscopy of both eyes showed retinal hemorrhage with cotton wool spots. Blood tests revealed increased titers of D-dimer and fibrin degradation products. The patient was followed up regularly at 2 weeks interval and BCVA and Fundus examination was carried out. At the end of 10 weeks, retinal hemorrhages had significantly cleared in both eyes with improvement of vision in both eyes (BCVA of right eye at last follow-up 6/6 and left eye 6/9).Bangladesh Journal of Medical Science Vol.14(3) 2015 p.297-298

Fibrinogen/Fibrin Degradation Products and Factor XIII

1974 ◽  
Vol 51 (6) ◽  
pp. 321-330 ◽  
Author(s):  
K. Miloszewski ◽  
M.J. Sheltawy ◽  
M.S. Losowsky
Keyword(s):  
Factor Xiii ◽  
Degradation Products ◽  
Fibrin Degradation Products

The Measurement of Fibrinolysis in the Rat

1971 ◽  
Vol 26 (02) ◽  
pp. 295-310 ◽  
Author(s):  
M. J Gallimore ◽  
H. M Tyler ◽  
J. T. B Shaw
Keyword(s):  
Whole Blood ◽  
Fibrinolytic Activity ◽  
Factor Xiii ◽  
Degradation Products ◽  
Blood Clot ◽  
Clot Lysis ◽  
Plate Assay ◽  
Fibrin Degradation Products ◽  
Fibrin Plate ◽  
Dilute Blood

SummaryMethods are described for measuring fibrinolytic activity in the rat. These include dilute blood clot lysis, euglobulin clot lysis, a fibrin plate assay with euglobulin solutions, and an accelerated whole blood clot lysis technique in which limited fibrinolysis is induced with urokinase. In addition, methods have been worked out for the estimation of four plasma components closely involved in fibrinolysis: fibrinogen, factor XIII, plasma inhibitors and fibrin degradation products. The sensitivity and validity of the assays were tested by studying their capacity to detect changes resulting from the administration of eledoisin, Neohydrin and turpentine.

A New Type of Congenital Dysfibrinogenemia (Fibrinogen Tokyo) with Defective Stabilization of Fibrin Polymers

1975 ◽  
Author(s):  
T. Samori ◽  
M. Yatabe ◽  
M. Ukita ◽  
M. Fujimaki ◽  
K. Fukutake
Keyword(s):  
Factor Xiii ◽  
Fibrinogen Level ◽  
Degradation Products ◽  
Immunological Methods ◽  
Thrombin Time ◽  
Normal Range ◽  
Plasma Factor ◽  
New Type ◽  
Abnormal Pattern ◽  
Assay Methods

A new type of congenital dysfibrinogenemia characterized by abnormal stabilization of fibrin polymers under the normal concentration of plasma factor XIII and normal thrombin time has been discovered in Tokyo.The molecular abnormality of this abnormal fibrinogen are shown by an anodal immunoelectrophoretic mobility and an abnormal pattern of D fragment in fibrinogen degradation products by plasmin digest on Immunoelectrophoresis and crossimmunoelectrophoresis.However, the plasma fibrinogen level of this case measures always in the normal range, when immunological methods or thrombin dependent methods are used, and the decreased level of plasma factor XIII is indicated by the use of assay methods based on clotlysis.

Thrombolysis with Streptokinase in Rabbits Dose Response, Fibrin-clot Specificity and Laboratory Evaluation of Fibrinolytic Effect

1974 ◽  
Vol 31 (02) ◽  
pp. 265-272
Author(s):  
Andrzej Nowak ◽  
Victor Gurewich
Keyword(s):  
Animal Model ◽  
Thrombolytic Therapy ◽  
Dose Response ◽  
Degradation Products ◽  
Fibrin Clot ◽  
Laboratory Evaluation ◽  
Clot Lysis ◽  
Excellent Correlation ◽  
Fibrin Degradation Products ◽  
High Degree

SummaryAn animal model was used in which venous thromboemboli of reproducible size could be formed. The effects of SK 500–50,000 units per hour were evaluated and compared to those of saline. Proportionately the greatest clot lysis occurred with the lowest doses of SK. In contrast to thrombolysis, significant fibrinogenolysis did not occur in any of the animals indicating a high degree of specificity of SK-induced activator for rabbit fibrin. An excellent correlation between the concentration of fibrin degradation products (fdp) measured by the serial dilution protamine sulfate test and the extent of clot lysis was found suggesting this to be a useful method for monitoring the effects of thrombolytic therapy. A method for the laboratory distinction of fdp from fibrin monomer was demonstrated.

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