scholarly journals EVALUASI HASIL INSEMINASI BUATAN DI BALAI PEMBIBITAN TERNAK UNGGUL DAN HIJAUAN PAKAN TERNAK INDRAPURI

2020 ◽  
Vol 3 (2) ◽  
pp. 73-78
Author(s):  
Aidilof, Sri Rahayu, Supriadi
Keyword(s):  
Conception Rate ◽  
Chi Square

Peternakan sapi lokal Aceh  rata-rata masih berskala kecil dan bersifat tradisional menyebabkan  produktivitas  ternak rendah. Salah satu cara untuk meningkatkan produktivitas adalah dengan memperbaiki kinerja reproduksi. Inseminasi Buatan merupakan salah satu upaya dalam meningkatkan produktivitas sapi Aceh. Penelitian ini dilaksanakan pada  bulan Januari sampai dengan Maret 2019 dengan lokasi penelitian  di Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak Indrapuri (BPTU-HPT Indrapuri).Penelitian ini bertujuan untuk mengetahui tingkat keberhasilan Insemiansi Buatan (IB) berdasarkan Service Per Conception (S/C) dan Conception Rate (CR). Data yang digunakan  adalah data kuantitatif  yang diperoleh  dari petugas dan catatan rekording dengan metode observasi dan wawancara kepada petugas dan pejabat yang  membidangi inseminasi buatan.Materi yang digunakan adalah sapi aceh yang di-IB pada tahun 2017 sebanyak 19 ekor dan 2018 sebanyak 22 ekor dengan pejantan yang berasal dari sapi aceh.Berdasarkan hasil analisa menunjukkan bahwa S/C sapi aceh di BPTU-HPT Indrapuri yaitu 1,12 dan CR 89,47% untuk tahun 2017. Dan untuk tahun 2018 diperoleh nilai S/C 1,16 dan CR 86,36%. Hasil perhitungan Chi-Square trhadap S/C antara tahun 2017 dan 2018 tidak menunjukkan perbedaan yang nyata (P 0,05). Sedangkan hasil uji Z terhadap CR sapi aceh untuk tahun 2017 dan 2018 juga tidak menunjukkan perbedaan yang nyata  antara tahun 2017 dan 2018.Hasil inseminasi buatan pada BPTU-HPT Indrapuri ini sangat baik bila dibandingkan dengan rata-rata normal S/C yaitu 1,6 – 2,0 dan CR yaitu 60-70 persen.Kata kunci : Inseminasi Buatan, Service Per Conception, Conception Rate

23 EFFECTS OF THE NUMBER OF SERVICES FERTILITY IN DAIRY COWS

2011 ◽  
Vol 23 (1) ◽  
pp. 118 ◽  
Author(s):  
M. Yamaguchi ◽  
M. Tanisawa ◽  
H. Koyama ◽  
S. Takahashi ◽  
O. Dochi
Keyword(s):  
Dairy Cows ◽  
Conception Rate ◽  
Low Fertility ◽  
Chi Square ◽  
The Third ◽  
Total Percentage ◽  
Conception Rates ◽  
Service Conception ◽  
Chi Square Test ◽  
Rectal Palpation

In recent years, reproductive performance of dairy cows has been declining worldwide, especially among cows of high genetic merit for milk production. The cause of the low fertility may considerably vary across countries and is probably multifactorial. This problem remains unsolved. Further, the first-service conception rate of dairy cows has remarkably decreased worldwide. The number of services required for conception has increased due to the low fertility in dairy cows. However, there are few reports about the relationship between conception rates and the number of services in the current dairy cows. The objective of this study was to investigate whether the number of services affects the conception rates of dairy cows. Data concerning the conception rates was obtained for 8386 Holstein cows from 40 commercial dairy herds in eastern Hokkaido, Japan, from 2006 to 2009. The diagnosis of pregnancy was confirmed by rectal palpation between 30 and 45 days after insemination. The average interval between calving and the first-service was 87.3 days. The average milk yield was 8500 kg. Number of conducting services was from the first to the seventh service. The conception rate was analysed using chi-square test. The results are presented in Table1. The conception rate from the first to the seventh service was 40.2 to 54.0%. The conception rate at the first service was significantly low (40.2%); however, the conception rates did not significantly differ after the second-service. A similar tendency was observed during each year. Moreover, the total percentage of conceptions from the first to the third service was 83.8%. The average number of services per conception was 2.2. The results of this study indicate that the first service yielded the lowest conception rates. The conception rates after the second service did not significantly differ. Moreover, ∼84% of the dairy cows became pregnant between the first service and the third service. However, this result shows that ∼16% of the dairy cows in the herds were repeat breeding. In order to improve fertility, it is necessary to study the factors that affect the first-service conception rates of dairy cows. Moreover, to improve the conception rate of dairy cows, it is important to elucidate the cause of these problems. Table 1.The number and percentage of dairy cows conceiving at each of the services for consecutive services

153 EFFECT OF DIFFERENT HOLDING AND TRANSPORT MEDIA ON CONCEPTION RATES FOLLOWING TRANSFER OF IN VIVO AND IN VITRO FERTILIZATION-DERIVED BOVINE EMBRYOS

2011 ◽  
Vol 23 (1) ◽  
pp. 180
Author(s):  
C. A. Zanenga ◽  
C. M. Martins ◽  
N. C. Rodovalho ◽  
F. Aidar ◽  
J. F. Hasler ◽  
...  
Keyword(s):  
Animal Health ◽  
Conception Rate ◽  
Bovine Embryos ◽  
Chi Square ◽  
Mato Grosso ◽  
Conception Rates ◽  
Chi Square Test ◽  
Donor Cows

Two experiments were conducted to compare conception rates following embryo transfer (ET) of bovine embryos held and transported in Syngro® holding medium (Bioniche, Belleville, Ontario, Canada) with other 2 holding media: Emcare® (ICPbio, Auckland, New Zealand) for in vivo-derived embryos and HEPES-buffered synthetic oviduct fluid (H-SOF) for IVF-derived embryos. The first trial was performed in the period from October through December 2006 at the Curitiba farm in Poços de Caldas, Minas Gerais, Brazil. A total of 140 in vivo-derived embryos were produced from 20 Nelore donor cows and transferred fresh at the same farm. After each donor recovery, embryos were equally separated per stage (morula or blastocyst) and classification (grades 1, 2, and 3) into 2 Petri dishes, each containing either Syngro or Emcare. The embryos were held for an average of 3 h after recovery, loaded into 0.25-mL straws, and transferred fresh into recipients heifers, which were all previously synchronized with the same hormonal protocol treatment and presented a corpus luteum on the day of transference. Conception rate was checked at approximately 60 days of conception by rectal palpation. The chi-square test was used for statistical analysis. The conception rate of embryos maintained in Syngro was significantly higher than those in Emcare: 64.2% (43/67) v. 47.9% (35/73; P < 0.05). A second experiment was performed between September and December 2008 at Embriza Biotechnology Laboratory, Campo Grande, Mato Grosso do Sul, Brazil. A total of 1689 IVF-derived embryos (stage = 7, quality = 1), produced from Nelore donor cows, were randomly assigned to be held and transported in either Syngro (769) or H-SOF transport medium (920). Transportation time ranged from 1 to 9 h, and the recipient farms ranged from 100 to 1200 km in distance from the Embriza Laboratory. Crossbred recipient heifers (Bos taurus × Bos indicus) were synchronized with prostaglandin or vaginal progesterone device protocols. Pregnancy diagnosis was performed by ultrasonography approximately 60 days after ET. Statistical comparisons were performed using the chi-square test. Conception rates resulting from embryos transported in Syngro (45.1%, 347/769) and in H-SOF (42.0%, 386/920) were not different (P = 0.19). Financial support from Embriza Biotecnology, Tecnopec LTDA, and Bioniche Animal Health

98 INFLUENCE OF INJECTION ALTRENOGEST IN UTERINE TONE AND EMBRYONIC VESICLE GROWTH IN RECIPIENT MARES

2017 ◽  
Vol 29 (1) ◽  
pp. 157
Author(s):  
V. H. Vallejo Aristizabal ◽  
H. D. Mogollón Garcia ◽  
J. A. Dell'aqua Junior
Keyword(s):  
Statistical Analysis ◽  
Corpus Luteum ◽  
Standard Error ◽  
Winter Season ◽  
Conception Rate ◽  
Chi Square ◽  
Recipient Group ◽  
The Mean ◽  
Made In

The objective of this study was to compare the influence of injectable altrenogest on uterine tone (UT) and embryonic vesicle growth (EVG) in non-cyclic recipient mares in anestrus and transition with cyclic mares. This study was conducted in Brazil in the winter season (June to September) between 2011 and 2014. One-hundred fifty-five embryo transfers (ET) were made in crossbreed mares from 4 to 10 years of age, between 350 and 450 kg of weight. The recipient mares were distributed into 3 groups: G1: anestrus (n = 67), mares with follicles less than 15 mm and absence of corpus luteum; G2: transition (n = 14), mares with follicles between 15 and 20 mm and absence of corpus luteum; and G3: cyclic (n = 74), mares with follicles larger than 20 mm and presence of corpus luteum. On Day 0 (day of donor mare ovulation), recipient mares in G1 and G2 received a single IM dose of 17β-oestradiol (10 mg). Recipients were examined by ultrasound on Days 1 and 2 to evaluate the presence of uterine oedema. On Day 2, once oedema was confirmed, recipients were given 180 mg of altrenogest IM, and altrenogest treatment was repeated every 8 days until Day 90 of gestation. The G3 recipient mares were not treated with 17β-oestradiol and altrenogest. For mares in G1 and G2, ET was performed 6 days after the initiation of altrenogest treatment. For recipients in G3, ET was performed between Days 6 and 8. Uterine tone was evaluated before ET on a scale of 0 to 3 (tonus absent 0, and tonus intense 3). Conception rate was determined by ultrasound at Day 15. The diameter of the EVG was calculated by measuring the length and width of the embryonic vesicle on Days 15, 20, 25, 30, 35, 40, and 45 using ultrasonography. Analysis of the variables UT and EVG was performed with Proc Mixed. Results are represented as ± standard error of the mean. Statistical analysis for conception rate was made by chi-square distribution adjusted with the Fisher’s test when it was required. All data were analysed with the SAS statistical package. There was no difference (P > 0.05) in UT between recipient groups (G1: 2.6 ± 0.04; G2: 2.6 ± 0.09; G3: 2.5 ± 0.04). In addition, there was no effect of recipient group nor an interaction between recipient group and day of gestation for EVG (P > 0.05). However, conception rate was greater (P < 0.05) in G2 (11/14 = 78%) and G3 (44/74 = 59%) compared with G1 (26/67 = 39%). Results of the present study demonstrate the effectiveness of injectable altrenogest for use in managing anestrus and transition mares for ET.

scholarly journals Pengaruh Deposisi Semen Terhadap Keberhasilan Inseminasi Buatan (IB) Sapi Peranakan Ongole

2017 ◽  
Vol 15 (2) ◽  
pp. 49
Author(s):  
Nilawati Widjaja ◽  
Tedi Akhdiat ◽  
Devi Purwasih
Keyword(s):  
Artificial Insemination ◽  
Research Data ◽  
Conception Rate ◽  
Chi Square ◽  
Non Parametric

<p><em>The research was aimed to analyze the effect of semen deposition in position of cervix utery, corpus utery, and cornua utery on the success of artificial insemination (AI) of Peranakan Ongole Catlle as well as to determine appropriate semen deposition which is able to yield the highest pregnancy in Peranakan Ongole Cattle. Data was analyzed with chi square Kruskal Wallis method within three kind of treatments, consisted of semen deposition on cervix utery (D1), semen deposition on corpus utery (D2), semen deposition on cornua utery (D3). Each treatment was replicated eight times. Conception rate and service per conception were observed in this research. Data were analyzed with non-parametric statistic. The results showed that CR value for D1, D2, and D3 were 12.5, 37.5, and 87.5%, respectively, and S/C values were 8.00, 2.60, and 1.14, respectively. This study concluded that semen deposition affected the success of artificial insemination (AI) in Peranakan Ongole cattle.  Semen deposition on cornua utery yielded the highest level of pregnancy in Peranakan Ongole cattle.</em></p>

368 CONCEPTION RATE AT FIRST ARTIFICIAL INSEMINATION WITH SEXED SEMEN OR CONVENTIONAL SEMEN IN CROSSBRED DAIRY COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 340
Author(s):  
B. T. Gerhardt ◽  
L. D. P. Sinedino ◽  
A. P. Dourado ◽  
P. A. M. Alves ◽  
L. A. G. Nogueira
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Genetic Selection ◽  
Conception Rate ◽  
Control Group ◽  
Chi Square ◽  
Fertility Reduction ◽  
Estrus Detection ◽  
Sexed Semen ◽  
Experimental Group

The use of sexed semen in dairy cattle almost eliminates the birth of unwanted male calves and can lead to increased milk production by means of genetic selection exerted on the females. However, AI with sexed semen still presents several obstacles to overcome. Holstein-Zebu cows are responsible for 80% of the milk produced in Brazil. These crossbred animals are used extensively throughout the country because their physiologic and morphologic characteristics are compatible with milk production in the tropics which results in an economic performance advantage. The objective of this study is to evaluate the conception rates with sexed semen, comparing it to the use of non-sorted semen in crossbred dairy cows. In addition, two insemination times had been studied to determine the best timing for AI with the sexed semen. Both studies were carried out in the Experimental Station Seropedica of Pesagro-RJ, located in the state of Rio de Janeiro, Brazil. The experiment was done in 2008/2009 (January to April). In the first Trial 56 Holstein-Zebu females, composed of 1/2 to 7/8 Holstein genetics, were inseminated 12 h after estrus detection with non-sorted semen (control group n = 29, 14 heifers and 15 cows) or with sexed semen (experimental group n = 27, 13 heifers and 14 cows). The semen utilized was from the same bull and thawing was performed intravaginally. In the second trial, 55 females were inseminated only with sexed semen from the same bull, with thawing in water at 35°C, at two different times: 12 h after estrus observation (control group n = 27, 12 heifers, 4 primiparous, and 11 multiparous) and 15 h after estrus observation (experimental group n = 28, 12 heifers, 5 primiparous, and 11 multiparous). The pregnancy diagnoses were done by rectal palpation and transrectal CAT scan at Day 45. Statistical analyses had been done using Chi-square. The conception rate in the first trial was of 17.2% (5/29) for sexed semen and 53.8% (14/26) for non-sorted semen (P < 0.05). In the second trial, the conception rate was of 37.0% (10/27) for insemination with sexed semen, 12 h after estrus observation and 50.0% (14/28) for the females inseminated 15 h after estrus observation (P > 0.05). It is possible that one of the causes of fertility reduction with sexed semen is that sexed-sperm remain viable for a shorter time after the insemination. Inseminations with sexed semen done 15 h after estrus detection, which can be more synchronized with the time of ovulation, and when accompanied by closely controlling the thawing process, show results similar to those obtained with conventional semen.

136 IMPACT OF SELECTION SYSTEM BY KINETICS ON THE EARLY EMBRYONIC DEVELOPMENT IN BOVINE OVUM PICKUP-IVF EMBRYOS

2017 ◽  
Vol 29 (1) ◽  
pp. 176
Author(s):  
M. Takayama ◽  
M. Moriyoshi ◽  
O. Dochi ◽  
K. Imai
Keyword(s):  
Embryo Transfer ◽  
Calf Serum ◽  
Cumulus Cells ◽  
Conception Rate ◽  
Culture Dish ◽  
Fresh Embryo Transfer ◽  
Chi Square ◽  
Predicting Factors

Recently, in vitro-produced (IVP) embryos have been increasingly produced using ovum pickup (OPU) and IVF in cows worldwide. However, the conception rate of IVP embryos is lower than that of in vivo-derived embryos. This study was conducted to determine the proportion of embryos that led to a high conception rate when the embryos were selected according to the 4 predicting factors. A total of 30 Holstein and 20 Japanese Black cows were used, and 81 OPU-IVF sessions were performed from October 2014 to May 2016. The collected cumulus-oocyte complexes (COC) were cultured for 22 h. Capacitated sperm (at a final concentration of 5 × 106 spermatozoa/mL) were incubated with COC for 6 h. After insemination, presumptive zygotes were separated from cumulus cells and sperm by pipetting. Then, the presumptive zygotes were cultured for 9 days in CR1aa supplemented with 5% calf serum by using a micro-well culture dish (Dai Nippon Printing, Tokyo, Japan). The kinetics of embryo development was observed at 27, 31, and 55 h post-insemination (hpi). The 4 factors used to select embryos were as follows: (1) time at which first cleavage occurred (less than 27 hpi, or less than 31 hpi, in case any of the zygotes did not cleave at 27 hpi in each culture dish); (2) 2 blastomeres after first cleavage at 31 hpi; (3) absence of fragments after first cleavage at 31 hpi; and (4) 8 or more blastomeres at 55 hpi. The number of blastocysts was analysed at 7, 8, and 9 days post IVF. Additionally, the number of produced embryos that could be used for embryo transfer (ET) was determined. The data were analysed using the chi-square test. The total numbers of blastocysts and produced embryos were 615 and 503, respectively. The numbers of blastocysts and produced embryos selected using the combination of factors 1 to 4 were 200 (32.5%) and 169 (27.5%), respectively. The numbers of blastocysts and produced embryos selected using factor 1 were 397 (64.6%) and 340 (67.6%), using factor 2 were 445 (71.3%) and 378 (75.1%), using factor 3 were 364 (81.8%) and 307 (81.2%), and using factor 4 were 374 (60.8%) and 308 (61.2%), respectively. The numbers of blastocysts and produced embryos that were rejected using a combination of factors 1 to 4 were 123 (27.5%) and 90 (17.9%), respectively. The conception rate of fresh embryo transfer was 46.6% (n = 73). We found that the conception rate of the embryos selected using factors 1 to 4 was significantly (P < 0.05) higher than that of embryos without one factor or more [60.0% (n = 35) v. 29.4% (n = 34)]. These results show the applicability and efficiency of the 4 factors for producing embryos with a high competence for conception.

15 Effects of Timed Artificial Insemination Protocol Using Prostglandin F2α and Estradiol Benzoate on Conception Rate in High-Producing Dairy Cows

2018 ◽  
Vol 30 (1) ◽  
pp. 147
Author(s):  
M. Yamaguchi ◽  
M. Takayama ◽  
H. López
Keyword(s):  
Reproductive Performance ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Conception Rate ◽  
Holstein Cows ◽  
Dairy Herds ◽  
Chi Square ◽  
Parity Number ◽  
Chi Square Test ◽  
Synchronization Protocol

For several years, milk production per Holstein cow has been increasing while reproductive performance has declined worldwide. Timed AI (TAI) protocols with synchronization of oestrus and ovulation are tools for the improvement of reproductive performance in high producing dairy herds. In protocols for synchronization of oestrus and ovulation, oestradiol benzoate (EB) has been used to increase the number of animals that show oestrous behaviour, synchronize ovulation, and improve conception rate (CR). The objective of this study was to compare a simple oestrus synchronization and TAI protocol using prostaglandin F2α (PG) and EB with a more complicated TAI protocol or oestrus detection and AI on CR in high-producing Holstein cows. The study was conducted from 2014 to 2016, using 1,036 Holstein cows from 3 commercial dairy herds in central Hokkaido, Japan. The average parity number was 2.6 ± 1.5, and days open averaged 152.2 ± 82.0 days. The average milk yield (305 days) was 11,348 ± 1,619 kg. Oestrus synchronization of cows was achieved using PG followed by EB and TAI (PG was given to cows with a cutoff for CL diameter of >20 mm by ultrasonography; EB (1 mg) was administered 24 h later and AI was carried out 24 to 48 h after EB injection), or by Heatsynch + CIDR [gonadotropin-releasing hormone (GnRH) injection and CIDR insertion at random stages of the oestrous cycle, CIDR removal, and PG injection 7 days later, EB injected 24 h after CIDR removal, and TAI 24 to 32 h after EB injection]. Cows observed in natural oestrus were used as controls. All cows were inseminated after the onset of standing heat or removal of the tail chalk (Paintstik®, LA-CO Industries, Elk Grove Village, IL, USA). Pregnancy diagnoses were performed using ultrasonography between 30 and 45 days after AI. The CR were compared using chi-square test. The CR (no. of pregnant cows/no. of total cows in the treatment group) of the natural oestrus group, PG + EB, and Heatsynch + CIDR cows were 39.2% (302/771), 38.7% (48/124), and 31.2% (44/141), respectively. Overall CR for the 3 groups was 38.0%. There were no significant differences in CR among the 2 groups (P > 0.07). These results show that a simple oestrus synchronization protocol with injection of PG followed by EB can be used effectively for high-producing dairy herds, yielding a satisfactory CR similar to that obtained with the Heatsynch + CIDR protocol or natural oestrus.

EFFECTS OF COLD STRESS AND REPEAT MATING ON REPRODUCTIVE PERFORMANCE OF SWINE

1972 ◽  
Vol 52 (2) ◽  
pp. 309-316 ◽  
Author(s):  
E. E. SWIERSTRA ◽  
G. W. RAHNEFELD
Keyword(s):  
Cold Stress ◽  
Litter Size ◽  
Reproductive Performance ◽  
Minimum Temperature ◽  
Conception Rate ◽  
Research Station ◽  
Chi Square ◽  
Ambient Temperatures ◽  
Temperature Ranges ◽  
Single Mating

The effects of cold stress, as measured by minimum ambient temperatures at day(s) of mating, on conception rate (CR), and litter size were studied in purebred Yorkshire and Lacombe swine populations maintained at the Canada Agriculture Research Station, Brandon, Manitoba (49°50′N, 99°57′W). One hundred and sixty-nine Yorkshire and 160 Lacombe boars were used to hand mate 406 Yorkshire gilts (505 estruses) and 460 Lacombe gilts (558 estruses). All gilts were bred on the 1st day of standing estrus, and gilts still in estrus the next day were mated a second time with the same boar. All matings were carried out in December and January over an 8-year period. Gilts and boars were housed separately in outside pens with wooden cabins as shelter. The data were grouped on the basis of minimum temperature on day(s) of mating into the following arbitrarily chosen temperature ranges: < −25, −25 to −18, −17 to −10 and > −10 C. The CR for gilts mated once were 81, 73, 58, and 56%, respectively, for the four temperature ranges. Corresponding values for the gilts mated more than once were 88, 81, 73, and 65%. Chi-square analyses indicated that CR were dependent (P < 0.01) on ambient temperatures. Repeat mating per estrus increased CR of the Yorkshire gilts by 12.5% (77.9 vs. 65.4%) and of the Lacombe gilts by 11.3% (80.4 vs. 69.1%) as compared with single mating. In both breeds, repeat mating increased litter size by 0.3 piglets (9.2 vs. 8.9, P = 0.10) over single matings. Litter size was not influenced by cold stress at time of mating.

scholarly journals EFFECT OF THE ADMINISTRATION OF SERUM AND RECOMBINANT EQUINE CHORIONIC GONADOTROPIN ON OVARIAN DYNAMICS AND CONCEPTION RATE IN ANESTRUS PRIMIPAROUS BRANGUS COWS

SPERMOVA ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 60-66
Author(s):  
Daniel Scandolo ◽  
◽  
Alejandra Cuatrin ◽  
Virginia Mazzuca ◽  
Mariano Finello ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Conception Rate ◽  
Ovulation Rate ◽  
Chi Square ◽  
Ovulatory Follicle ◽  
Chi Square Test ◽  
Follicular Size ◽  
Equine Chorionic Gonadotropin ◽  
And Control ◽  
Ovarian Dynamics

The objective was to determine the size of the dominant ovulatory follicle, the ovulation time and the conception rate of cows in anestrus treated with serum equine chorionic gonadotropin (eCG), recombinant chorionic gonadotropin and untreated controls. 57 anestrus primiparous Brangus cows were used with 70 ± 26 days of calving and a body condition of 2.50 ± 0.15. They were synchronized with a FTAI protocol based on estrogen and progesterone devices (DI). Upon removal of DI, 3 groups were made according to the type of eCG applied: serum eCG received 400 IU im of equine Chorionic Gonadotropin, recombinant eCG received 140 IU of recombinant Equine Chorionic Gonadotrophin and Control did without treatment. Follicular size and time of ovulation (normal or abnormal) was determined at DI removal, during FTAI and 7 days later. An ANOVA was performed to determine the effect of the treatment on ovarian dynamics and a chi-square test and correspondence analysis to establish associations. In normal ovulation cows at the FTAI, 94.4% of the serum eCG, 55.5% of the recombinant eCG and 71.4% of the Control cows presented a dominant ovulatory follicle (DOF), which in those treated with serum eCG, it was 1.4 mm greater in relation to the Control (P = 0.0073). The ovulation rate, in normal and abnormal ovulation cows, was 94.4% for serum eCG, and for recombinant eCG and 66.6% for Controls. A significant association was detected between ovulation time and the treated groups (P = 0.0042). Normal ovulation was 38.9% higher in cows with serum eCG in relation to those treated with recombinant eCG and 50.8% in comparison with Control. A significant relationship was observed between the conception rate and the groups treated with the different Gonadotropins (P = 0.0574), being 66.7% in serum eCG, 50% in recombinant eCG and 28.6% in the Control. The use of eCG, either serum or recombinant, in anestrus primiparous cows, stimulates the development of DOF at FTAI, increases ovulation rate and improves conception in relation to untreated cows.

11 Conception rate of high-producing dairy cows at first service using prostaglandin F2α and oestradiol benzoate

2019 ◽  
Vol 31 (1) ◽  
pp. 131
Author(s):  
M. Yamaguchi ◽  
M. Takayama ◽  
H. López ◽  
O. Dochi
Keyword(s):  
New York ◽  
Dairy Cows ◽  
Corpus Luteum ◽  
Animal Health ◽  
Prostaglandin F2α ◽  
Conception Rate ◽  
Chi Square ◽  
Average Interval ◽  
Oestradiol Benzoate ◽  
Days Open

We have shown that a simplified oestrus synchronization protocol using prostaglandin F2α (PG) and oestradiol benzoate (EB) resulted in a satisfactory conception rate, similar to that obtained in high-producing dairy cows with natural oestrus (Yamaguchi et al. 2018 Reprod. Fertil. Dev. 30, 147 abstr). However, we found no significant effect of PG+EB protocols on the first-service conception rates (CR) of dairy cows. This study investigated the effect of the first-service CR by using PG+EB protocols in the high-producing dairy cows that had not exhibited oestrus expression after calving. Data concerning the first-service CR were obtained for 481 Holstein cows from 4 commercial dairy herds in Hokkaido and Ibaraki, Japan, from 2014 to 2017. The average number of parity, average interval between calving and the first service, and average milk yield (at 305 days) of the cows were 2.2±1.3, 89.7±26.1 days, and 10314±1994kg, respectively. We carried out the PG+EB protocols on cows not showing evident standing heat from Days 50 to 150 postpartum regardless of the presence of a corpus luteum in the ovary. The statuses of ovaries of cows were confirmed using ultrasonography before starting the treatment. Prostaglandin F2α (25 mg; Lutalyse, Pfizer, New York, NY, USA) was injected into cows with a &gt;20mm cutoff for functional corpus luteum diameter at a random stage of oestrus, and EB (1 mg; Ovahormon®, Aska Animal Health Co., Tokyo, Japan) was injected 24h later, and AI was carried out 24 to 48h after EB injection. Cows with a natural oestrus service from Days 50 to 150 postpartum were used as control. All the cows were inseminated after the onset of standing heat or removal of the tail chalk. Pregnancy diagnoses were performed using ultrasonography 30 to 45 days after AI. The CR and intervals of partum to conception were analysed using a chi-square test and ANOVA. The first-service CR (i.e. the number of pregnant cows/total number of cows in the treatment group) of the natural oestrus (n=330) and PG+EB (n=151) groups were 42.7 and 41.1%, respectively, and there were no significant differences between these 2 groups (P=0.7). The average interval between calving and the first service of the PG+EB groups was significantly longer than that of the natural oestrus groups (86.4±1.5 days v. 96.9±1.9 days; P&lt;0.01). The average days open of the natural oestrus and PG+EB groups were 88.5±3.2 days and 98.1±2.3 days, respectively (P&lt;0.05). These results confirmed that the first-service CR in the PG+EB and natural oestrus groups of high-producing dairy cows were similar. We suggest that conducting PG+EB protocols was effective in the high-producing dairy cows that had not exhibited oestrus expression after calving. Moreover, using PG+EB protocols could prevent the further increase in the number of days open.

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