scholarly journals Prevalence of cryptococcal meningitis in a tertiary care centre.

2020 ◽  
Vol 7 (3) ◽  
pp. 15-21
Author(s):  
Ashish Bajaj ◽  
Bibhati Mishra ◽  
Poonam Loomba ◽  
Archana Thakur ◽  
Abha Sharma ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cryptococcal Meningitis ◽  
Tertiary Care ◽  
Cns Infection ◽  
Fungal Culture ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Cryptococcal Antigen ◽  
India Ink ◽  
Appropriate Treatment

Background: Cryptococcal meningitis has emerged as an important opportunistic central nervous system (CNS) infection in Human Immunodeficiency Virus (HIV) positive patients. It is associated with a high mortality rate. Hence early diagnosis is necessary to start appropriate treatment. Cryptococcosis is generally found in association with acquired immunodeficiency syndrome (AIDS) although it has been reported to cause disease in HIV-seronegative patients also. Objective:Prevalence of Cryptococcal meningitis in a tertiary care centre. Material & Methods: A total of 93 Cerebrospinal fluid samples from suspected cases of fungal meningitis were received in the microbiology department of GB Pant Hospital(GIPMER) from January to June 2018. Samples were subjected to direct microscopy- wet mount, India ink preparation and Gram stain, Cryptococcal antigen detection(Latex agglutination), and Fungal culture. In vitro susceptibility of Cryptococcus isolates to Fluconazole, Voriconazole, 5- Flucytosine and Amphotericin B was performed using standard broth microdilution method. Results: Out of 93 CSF samples, 6 were positive for India ink preparation and showed gram positive budding yeast cells by gram staining. All 6 samples were positive for the cryptococcal antigen test and fungal culture. Five were identified as C. neoformans and one C. gatti. All strains were susceptible to Amphotericin B. Four patients were HIV reactive and succumbed to the diseaseduring treatment. Two patients were found to be coinfected with Hepatitis B virus. Ashish Bajaj1, Bibhabati Mishra2, Poonam S Loomba2, Archana Thakur3, Abha Sharma4, Prachala G Rathod1, Madhusmita Das1, Ashna Bhasin1 1Senior Resident, 2Director Professor, 3Director Professor & Head, 4Assistant Professor, Department of Microbiology, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research (GIPMER), New Delhi, India ABSTRACT Cryptococcal meningitis has emerged as an important opportunistic central nervous system (CNS) infection in immunosuppressed patients.It is associated with a high mortality rate(>30%) in immunosuppressed patients especially those infected with HIV, hence early diagnosis is necessary to start appropriate treatment. Prevalence of cryptococcal infection is increasing in developing nations including India as per some recent studies.(1) Conclusion: Index of suspicion of Cryptococcus infection as a possible cause of meningitis must be considered in chronic meningitis cases. Microscopy (India ink preparation) may be used as a cheap and rapid diagnostic tool.

scholarly journals Prevalence of cryptococcal meningitis at a tertiary care centre in Western India (1996–2005)

2006 ◽  
Vol 55 (9) ◽  
pp. 1301-1302 ◽  
Author(s):  
Meena G. Satpute ◽  
Nilima V. Telang ◽  
Geetanjali M. Litake ◽  
Krishna B. Niphadkar ◽  
Suresh G. Joshi
Keyword(s):  
Cryptococcal Meningitis ◽  
Tertiary Care ◽  
Western India ◽  
Care Centre ◽  
Tertiary Care Centre

scholarly journals Intraoperative Squash Smear in Central Nervous System Tumors and Its Correlation with Histopathology: 1 Year Study at a Tertiary Care Centre

2017 ◽  
Vol 08 (02) ◽  
pp. 221-224 ◽  
Author(s):  
Arpita Jindal ◽  
Himanshi Diwan ◽  
Kanwalpreet Kaur ◽  
V. D. Sinha
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Partial Correlation ◽  
Tertiary Care ◽  
Central Nervous System Tumors ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Reliable Technique ◽  
Hematoxylin And Eosin ◽  
Time Of Operation

ABSTRACT Background: Intraoperative squash smear cytology is a simple and reliable technique for rapid intraoperative diagnosis of neurosurgical specimens. The study was designed to assess the accuracy of intraoperative squash smear in the diagnosis of central nervous system (CNS) space-occupying lesions. Materials and Methods: One hundred and fifty cases of CNS space-occupying lesion were studied in a period of 15 months. Smears were prepared from the biopsy sample obtained at the time of operation and were stained with rapid hematoxylin and eosin method. Results: One hundred and forty-one cases showed complete correlation with histopathology, two cases showed partial correlation, and seven cases were discrepant. The overall diagnostic accuracy was found to be 94%. Conclusion: Intraoperative squash smear thus provides diagnosis with fair accuracy in brain tumors and is of great value in intraoperative consultation.

A CLINICOPATHOLOGICAL STUDY ON DISSEMINATED HISTOPLASMOSIS WITH SPECIAL REFERENCE TO ATYPICAL CASES IN A TERTIARY CARE CENTRE -IN EASTERN INDIA.

2021 ◽  
pp. 48-51
Author(s):  
Anuradha De (Pati) ◽  
Roopsa Chakraborty ◽  
Bibhuti Saha
Keyword(s):  
Histoplasma Capsulatum ◽  
Histopathological Examination ◽  
Tertiary Care ◽  
Cd4 Count ◽  
Yeast Cells ◽  
Steady Increase ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Disseminated Histoplasmosis ◽  
India Ink

INTRODUCTION: Untreated Histoplasmosis is fatal for all, immunocompetent or immunocompromised. Histoplasmosis has seen a steady increase in incidence from a single recorded case in Kolkata, India in 1954 to 388 cases from 2004-17 and 161 from 2017-till date all over India. Yet, it has been consistently relegated to a niche of opportunistic infections without a proper diagnostic protocol. To study the clinical OBJECTIVES: features and natural history of disseminated histoplasmosis(DH) and analysis of cases with atypical clinical presentation of Histoplasmosis simulating malignancy in search of a consolidated and efcient diagnostic algorithm in a tertiary care centre. MATERIALS AND METHODS: The retrospective institution-based study was conducted in the Department of Pathology, CSTM from February 2011 to July 2019 in collaboration of Dept. of Tropical Medicine on specimens collected from 33 cases of Skin biopsy, Lymph node biopsy and FNAC aspirate from enlarged lymphnodes, slit skin smear followed by BM Aspirate analysis done in cases of disseminated histoplasmosis, Bone Marrow Biopsy was done in selected cases. Histoplasma capsulatum like yeast cells were detected with Leishman Giemsa stained slit skin smears in 9 cases and in FNA smears in 13cases and they were PAS positive. Diagnosis were conrmed in Histopathological examination along with special stain for fungus in all cases. Radiological investigations (CXR, USG, CT), Routine investigations (Haemogram, LFT, RFT, Electrolytes) along with CD4 count in ART clinic and microbiological tests like Gram stain of SSS, India Ink, KOH preparation, Cryptococcal Ag testing, urine C/S) were corroborated with. RESULTS: All 33 cases FNAC (13),slit skin smear(9) and HPE on rest of the specimen show small, oval yeast cells on LG stain. Biopsy showed histiocytic phagocytosis of yeast cells with foamy macrophages and PMN inltration with NO granulomas in seroposive cases. Gomori Methenamine Silver stain of BM biopsy showed black intracellular yeast consistent with . PAS stained FNAC smears Histoplasma capsulatum showed yeast cells with chromatin dot and surrounding halos with no kinetoplasts. Mycological cultures were in agreement. Here, in most cases, CD4 count and Disseminated Histoplasmosis ndings were not in accordance. DH CONCLUSION: is not uncommon in India and is being increasing day by day and it should be considered in the diagnosis of patients with prolonged fever, weight loss, oropharyngeal ulcers, hepatosplenomegaly, lymphadenopathy and adrenal enlargement. Correct diagnosis by well designed analytical studies utilising appropriate diagnostic modalities in an immunocompromised patient even with minimum suspicion of opportunistic infection, a consolidation of selective investigations like FNAC, HPE and relevant microbiological tests into a diagnostic design for maximum utilization of nances, resources and time, all of which can help to understand the epidemiology of this neglected disease for accurate treatment leading to a favourable outcome.

scholarly journals Mycology of Chronic Suppurative Otitis Media at Tertiary Care Centre of Nepal

2021 ◽  
Author(s):  
Ajay Kumar Chaurasiya ◽  
Rabindra Bhakta Pradhananga ◽  
Dipendra Kumar Mandal ◽  
Manoj Mahato ◽  
Niranjan Prasad Sah ◽  
...  
Keyword(s):  
Otitis Media ◽  
Potassium Hydroxide ◽  
Tertiary Care ◽  
Neck Surgery ◽  
Chronic Suppurative Otitis Media ◽  
Fungal Culture ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Suppurative Otitis Media ◽  
Positive Growth

Abstract ObjectiveThis study was designed to find out the fungal aetiological agents in chronic suppurative otitis media (CSOM) patients attending tertiary care centre of Nepal. ResultTotal 123 samples of 117 patients, outdoor as well as indoor from Department of ENT and Head and Neck Surgery (HNS) TUTH, Maharajgunj, Kathmandu those specimens were processed and among them, 23(18.7%) was found potassium hydroxide (KOH) mount positive whereas positive growth was in 27 specimens. The prevalence rate of fungus was 21.95 percent in which the main pathogen was Aspergillus species (51.8%), followed by Candida species (14.8 %). Keywords: CSOM, KOH, Fungal culture, Aspergillus

scholarly journals Prevalance and distribution of candidia species from diabetic foot ulcer in tertiary care centre, Jamnagar, Gujarat

2021 ◽  
Vol 11 (2) ◽  
pp. 231-235
Author(s):  
Binita J Aring ◽  
Pushpa R Kateshiya ◽  
Dipali Maganbhai Gavali
Keyword(s):  
Diabetic Foot ◽  
Fungal Pathogens ◽  
Tertiary Care ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Fungal Culture ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Early Recovery ◽  
Wound Discharge

Diabetes Mellitus is a chronic disease which may cause diabetic foot ulcer, which is a major cause of morbidity and mortality, it may also lead to foot amputation due to gangrene, and may cause cellulitis, abscess etc.To study prevalence of candidiasis in diabetic foot ulcer in a tertiary care centre, Jamnagar.32(10.66%) isolates that were recovered from wound discharge samples (300 samples tested) from November 2017 to September 2018. All isolates were visualized under direct microscopy, cultured, & sugar assimilation tests were performed.Amongst 300 samples 32(10.66%) were positive for fungal culture, in which major isolates was C. albicans (50%), C. tropicalis (18.75%), C. dubliniensis (9.37%), C.krusei (9.37%), C. glabrata (6.25%), C. parapsilosis (6.25%).This study shows that in Diabetic foot ulcer most common fungal pathogens were C. Albicans, C. tropicalis, C. dubliniensis, etc. Early identification of organism can help in early treatment and early recovery.

Sign in / Sign up

Export Citation Format

Share Document