Uji Aktivitas Antikolesterol Partisi N-Heksana, Metanol Dan Ekstrak Etanol Kulit Jeruk Nipis (Citrus Aurantiifolia) Secara In Vitro

AbstractCholesterol is an important sterol in human body tissue which belongs to the lipid group but cannot be hydrolyzed. Cholesterol has various uses in the body such as forming steroid hormones in the hormones estrogen and progesterone. However, if cholesterol levels in the blood are too high, it can cause blockage of blood flow which can lead to atherosclerosis. Lime contains secondary metabolites that function to reduce the increase in cholesterol levels in the blood. The purpose of this study was to determine the anticholesterol activity and to determine the EC₅₀ value of partition n-hexane, methanol, ethanol extract of lime peel (Citrus aurantiifolia) in vitro. Analysis of cholesterol activity is known by measuring cholesterol levels in vitro using Lieberman Burchard reagent. The analytical method used UV-Vis spectrophotometry at a wavelength of 665.0 nm with a series of test sample concentrations of 150 µg/ml; 300 µg/ml; 450 µg/ml; 600 µg/ml and 750 µg/ml. The research data shows that the decrease in cholesterol levels is directly proportional to the increase in the concentration in the sample. The EC₅₀ value of the n-hexane partition was 448.76 µg/ml; methanol partition as much as 448.98 µg/ml and ethanol extract as much as 450.18 µg/ml.Keywords: Anticholesterol; in vitro; lime peel; partition. AbstrakKolesterol merupakan sterol yang penting dalam jaringan tubuh manusia yang termasuk pada golongan lipid tetapi tidak dapat terhidrolisis. Kolesterol memiliki berbagai kegunaan dalam tubuh seperti pembentuk hormon-hormon steroid pada hormon esterogen dan progrsteron. Namun, jika kadar kolesterol dalam darah terlalu tinggi maka dapat menyebabkan penyumbatan aliran darah yang dapat mengakibatkan penyakit Aterosklerosis. Jeruk nipis memiliki kandungan metabolit sekunder yang berfungsi untuk mengurangi kenaikan kadar kolesterol dalam darah. Tujuan pada penelitian ini yaitu mengetahui aktivitas antikolesterol dan mengetahui nilai EC₅₀ dari partisi n-heksana, metanol, ekstrak etanol kulit jeruk nipis (Citrus aurantiifolia) secara in vitro. Analisis aktivitas kolesterol diketahui dengan mengukur kadar kolesterol secara in vitro menggunakan pereaksi Lieberman Burchard. Metode analisis menggunakan spektrofotometri UV-Vis pada panjang gelombang 665,0 nm dengan seri konsentrasi sampel uji 150 µg/ml; 300 µg/ml; 450 µg/ml; 600 µg/ml dan 750 µg/ml. Data penelitian menunjukkan penurunan kadar kolesterol berbanding lurus dengan peningkatan konsentrasi pada sampel. Nilai EC₅₀ partisi n-heksana sebanyak 448,76 µg/ml; partisi metanol sebanyak 448,98 µg/ml dan ekstrak etanol sebanyak 450,18 µg/ml. Kata kunci: Antikolesterol; in vitro; kulit jeruk nipis; partisi.

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Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering

Jurnal Kimia Sains dan Aplikasi ◽

10.14710/jksa.21.2.54-58 ◽

2018 ◽

Vol 21 (2) ◽

pp. 54-58 ◽

Cited By ~ 2

Author(s):

Devina Ingrid Anggraini ◽

Lily Fathrah Nabillah

Keyword(s):

Leaf Extract ◽

Ethanol Extract ◽

The Body ◽

Flavonoid Content ◽

Cholesterol Lowering ◽

Cholesterol Levels ◽

Method Of Extraction ◽

Lowering Activity ◽

Solvent Analysis

Cholesterol is a natural substance with physical characteristic similar to fat but has a steroidal group. The body requires cholesterol in normal amount; however, it will harm the body in excess amount. High cholesterol levels in the blood are dangerous because of the precipitation of cholesterol and other fatty substances resulting in atherosclerosis. Suji leaf (Dracaena angustifolia Roxb.) used as a natural dye has a high flavonoid content that is inferred to have cholesterol-lowering activity. This study aims to test the in vitro activity of suji leaf (Dracaena angustifolia Roxb.) extract in decreasing cholesterol level with various concentrations and to find the effective concentration (EC50). The method of extraction used was remaceration method with 70% ethanol solvent. Analysis of cholesterol-lowering activity was done by Lieberman-Burchard method by making variation of ethanol extract 400 ppm, 500 ppm, 600 ppm, 700 ppm, and 800 ppm. The results showed the percentage of cholesterol-lowering activity by 33.62%, 36.15%, 46.61%, 56.39% and 64.05% respectively. Value of EC50 activity of suji leaf extract is 632.50 ppm.

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Disposal of Uranium and Thorium Entering the Human Digestive System before Reaching the Blood by Adsorption on Simethicone and Forming a Non-absorbable Compound: A Full in vitro Analysis

Indian Journal of Pharmaceutical Education and Research ◽

10.5530/ijper.55.1.1 ◽

2021 ◽

pp. 1-7

Author(s):

Hamed Ibrahim Mira ◽

Nashat Mohamed Alanwar Abdalaty

Keyword(s):

Total Concentration ◽

Reference Method ◽

The Body ◽

Compound A ◽

Icp Ms ◽

Human Digestive System ◽

Vitro Analysis ◽

Safe Dose ◽

In Vitro Analysis

Introduction: This method was applied to form a non-absorbable complex with uranium and thorium swallowed with contaminated dust or other sources to get rid of them before absorption into the blood and discarded outside the body. A non-GIT absorbable simethicone was used in this method for the adsorption of uranium (VI) and thorium (IV) from a solution similar to the gastrointestinal tract pH medium. Materials and Methods: Accurately measured 9 mg of pure standard uranyl nitrate and simethicone emulsion in concentration range (100-300 mg) at 37±1°C for 20 min in pH ranges like the medium of the stomach and intestine then take the supernatant after centrifugation to make sure of decreasing of the concentration of uranium and thorium ions by ICP Mass Spectroscopy (ICP-MS). Results and Discussion: The data recorded for different total uranium and thorium concentrations reveal convergent behavior. Increasing simethicone concentration inside the safe dose shifts the sorption of uranium and thorium to 100 % of the total concentration, calculated t and F values were compared with the reference method and the proposed method was equally precise and accurate. Conclusion: The data indicate that the method can successfully be applied for the complete adsorption of uranium and thorium on simethicone from GIT before reaching the blood.

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Suppression of Inflammatory Mediators by the Ethanol Extract of Crotalaria verrucosa L. leaf – in vivo and in vitro Analysis

European Journal of Medicinal Plants ◽

10.9734/ejmp/2016/21894 ◽

2016 ◽

Vol 11 (3) ◽

pp. 1-7

Author(s):

Kashfia Nawrin ◽

Mohammad Billah ◽

Mohammed Jabed ◽

Mohammad Khalil ◽

Md. Uddin ◽

...

Keyword(s):

Inflammatory Mediators ◽

Ethanol Extract ◽

Vitro Analysis ◽

In Vitro Analysis

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The native structure of the eukaryotic ribosome

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075890 ◽

1983 ◽

Vol 41 ◽

pp. 432-433

Author(s):

R.A. Milligan ◽

P.N.T. Unwin

Keyword(s):

Ribosomal Proteins ◽

Crystal Form ◽

Native Structure ◽

X Ray Diffraction ◽

Eukaryotic Ribosome ◽

Vitro Analysis ◽

In Vitro Analysis ◽

Resolution Structure ◽

Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

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1163: Radial Dilation of Ureteral Balloons: A Comparative in-Vitro Analysis

The Journal of Urology ◽

10.1016/s0022-5347(18)35308-4 ◽

2005 ◽

Vol 173 (4S) ◽

pp. 315-316

Author(s):

Kari Hendlin ◽

Brynn Lund ◽

Manoj Monga

Keyword(s):

Vitro Analysis ◽

In Vitro Analysis

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An In Vitro Analysis of Ligament Reconstruction or Extension Osteotomy on Trapeziometacarpal Joint Stability and Contact Area

Yearbook of Hand and Upper Limb Surgery ◽

10.1016/s1551-7977(08)70357-1 ◽

2007 ◽

Vol 2007 ◽

pp. 214-215 ◽

Cited By ~ 1

Author(s):

B. Wilhelmi

Keyword(s):

Contact Area ◽

Ligament Reconstruction ◽

Joint Stability ◽

Trapeziometacarpal Joint ◽

Vitro Analysis ◽

In Vitro Analysis

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Role of the 807 C/T Polymorphism of the α2 Gene in Platelet GP Ia Collagen Receptor Expression and Function

Thrombosis and Haemostasis ◽

10.1055/s-0037-1614605 ◽

1999 ◽

Vol 81 (06) ◽

pp. 951-956 ◽

Cited By ~ 59

Author(s):

J. Corral ◽

R. González-Conejero ◽

J. Rivera ◽

F. Ortuño ◽

P. Aparicio ◽

...

Keyword(s):

Venous Thrombosis ◽

Cell Types ◽

Receptor Expression ◽

Case Control Studies ◽

Platelet Surface ◽

Vitro Analysis ◽

And Function ◽

In Vitro Analysis

SummaryThe variability of the platelet GP Ia/IIa density has been associated with the 807 C/T polymorphism (Phe 224) of the GP Ia gene in American Caucasian population. We have investigated the genotype and allelic frequencies of this polymorphism in Spanish Caucasians. The T allele was found in 35% of the 284 blood donors analyzed. We confirmed in 159 healthy subjects a significant association between the 807 C/T polymorphism and the platelet GP Ia density. The T allele correlated with high number of GP Ia molecules on platelet surface. In addition, we observed a similar association of this polymorphism with the expression of this protein in other blood cell types. The platelet responsiveness to collagen was determined by “in vitro” analysis of the platelet activation and aggregation response. We found no significant differences in these functional platelet parameters according to the 807 C/T genotype. Finally, results from 3 case/control studies involving 302 consecutive patients (101 with coronary heart disease, 104 with cerebrovascular disease and 97 with deep venous thrombosis) determined that the 807 C/T polymorphism of the GP Ia gene does not represent a risk factor for arterial or venous thrombosis.

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