scholarly journals Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR.

2010 ◽  
Vol 15 (1) ◽  
Author(s):  
  Rangoju P.K. ◽  
Kumar S. ◽  
Kolte A.P. ◽  
Gulyani R. ◽  
Singh V.K.
Keyword(s):  
Genetic Variability ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Pcr

scholarly journals Genetic variability of Pantaneiro horse using RAPD-PCR markers

2007 ◽  
Vol 36 (4) ◽  
pp. 799-806 ◽  
Author(s):  
Andréa Alves do Egito ◽  
Beatriz Helena Fuck ◽  
Concepta McManus ◽  
Samuel Rezende Paiva ◽  
Maria do Socorro Maués Albuquerque ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Jaccard Coefficient ◽  
Mato Grosso ◽  
Rapd Pcr ◽  
Close Relationship ◽  
Polymerase Chain ◽  
Genic Diversity ◽  
Mato Grosso Do Sul

Blood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic bands were generated. The analysis of molecular variance (AMOVA) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75.47%). Analysis of the genetic variability between pairs of populations presented higher estimates for the five Pantaneiro populations with the Arabian breed, while lowest estimates were presented by pairs formed among the Pantaneiro populations with the Mangalarga Marchador. Highest genic diversity was shown by the Pantaneiro (0.3396), which also showed highest genetic distance with the Arabian and lowest with Mangalarga Marchador breed. UPGMA dendrogram showed distinct differences between naturalized (Pantaneiro and Mangalarga Marchador) and exotic (Arabian and Thoroughbred) breeds. In the dendrogram generated by UPGMA method, the similarity matrix generated by the Jaccard coefficient showed distinction between the naturalised breeds, Pantaneiro and Mangalarga Marchador, and the exotic breeds, Árab and English Thoroughbred. Results suggest that the Pantaneiro presents a higher genetic variability than the other studied breeds and has a close relationship with the Mangalarga Marchador.

Genetic variability estimated with RAPD-PCR markers in two populations of Heterodera glycines Ichinohe, 1952 (Nematoda: Heteroderidae) from Argentina

Nematology ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Paola Lax ◽  
Juan Rondan Dueñas ◽  
Cristina Gardenal ◽  
Marcelo Doucet
Keyword(s):  
Genetic Variability ◽  
Management Practices ◽  
Heterodera Glycines ◽  
Control Strategies ◽  
Random Amplified Polymorphic Dna ◽  
Allele Frequencies ◽  
Economic Losses ◽  
Genetic Population ◽  
Rapd Pcr ◽  
Two Populations

Abstract Knowledge of genetic variability within and between phytophagous nematode populations is important for the selection of suitable control strategies. The nematode Heterodera glycines is the most important pathogen of soybean in many producer countries, causing significant economic losses. The levels of variability were analysed in two populations of the nematode from Argentina, using Random Amplified Polymorphic DNA markers in order to evaluate the genetic population structure of this species. DNA was extracted from single females of race 1 and race 3 populations. Five primers were selected and 50 consistent fragments were considered for further analysis. Populations studied showed high levels of genetic diversity. Most of the total variation detected in allele frequencies was attributed to variability among individuals of the same population (83%) rather than inter-population differences (17%). None of the populations demonstrated exclusive bands. However, 68% of fragments showed significant differences in their allele frequencies. This study revealed an important degree of genetic differentiation between both populations, probably as a consequence of limited gene flow between them or because each population was under different management practices at its site of origin. Results herein represent the first analysis of genetic structure in H. glycines populations using RAPD-PCR technique.

scholarly journals Random Amplified Polymorphic DNA (RAPD) Analysis to Determine the Genetic Variability among Virulent and Less Virulent Isolates of Fusarium moniliforme, Fusarium oxysporum and Fusarium solani Isolated from Infected Cotton Seedlings

2016 ◽  
Vol 4 (3) ◽  
pp. 137-145
Author(s):  
Ahmed Abdelmagid ◽  
Abdel-Moneim Amein ◽  
Mohamed Hassan ◽  
Hamdy E. Hares
Keyword(s):  
Genetic Variability ◽  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Random Amplified Polymorphic Dna ◽  
Pathogenicity Test ◽  
Test Results ◽  
Fusarium Spp ◽  
Rapd Pcr ◽  
Highly Virulent ◽  
Cotton Cultivar

Root-rot of cotton (Gossypium spp.) is one of the most important diseases in Upper Egypt. Isolation has been done from diseased cotton roots and seeds which were collected from 11 counties in Assiut province, Egypt. Identification procedures of the isolated fungi confirmed that the isolated fungi were for Fusarium solani, F. moniliforme and F. oxysporum and Rhizoctonia solani. Thirty six isolates of Fusarium spp. and 10 isolates of Rhizoctonia solani were tested for their pathogenicity on both Giza 80 and Giza 83 cotton seedlings to verify their virulence on seedlings. The pathogenicity test results have grouped the Fusarium spp. isolates into three groups; highly virulent that caused 91-100% mortalities; moderately virulent that caused 81-90% mortalities and low virulent that caused lower than 81.0%  mortalities. Data also shows that, in general Giza 80 cotton cultivar was more susceptible for infection with Fusarium spp. when compared with Giza 83 cotton cultivar. In case of Rhizoctonia solani, data revealed that the infection percentage was significantly affected by isolates while cotton cultivars had no significant influence on infection. Four 10-mer primers (1:6-d, 2:6-d, 4:6-d and 5:6-d) were used in RAPD-PCR to determine the genetic variability between six isolates, one virulent and one less virulent, of F. moniliforme, F. oxysporum and F. solani. Our results showed that the primer 2:6-d clearly separated F. moniliforme, F. oxysporum and F. solani and proved to be quite powerful in distinguishing the three different species and isolates of Fusarium spp.

scholarly journals Oenological Potential of Autochthonous Saccharomyces cerevisiae Yeast Strains from the Greek Varieties of Agiorgitiko and Moschofilero

Beverages ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 27
Author(s):  
Dimitrios Kontogiannatos ◽  
Vicky Troianou ◽  
Maria Dimopoulou ◽  
Polydefkis Hatzopoulos ◽  
Yorgos Kotseridis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alcoholic Fermentation ◽  
Ethanol Tolerance ◽  
Random Amplified Polymorphic Dna ◽  
Yeast Strains ◽  
Rapd Pcr ◽  
Autochthonous Yeast ◽  
Polymerase Chain ◽  
Grape Varieties ◽  
H2s Production

Nemea and Mantinia are famous wine regions in Greece known for two indigenous grape varieties, Agiorgitiko and Moschofilero, which produce high quality PDO wines. In the present study, indigenous Saccharomyces cerevisiae yeast strains were isolated and identified from spontaneous alcoholic fermentation of Agiorgitiko and Moschofilero musts in order to evaluate their oenological potential. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) recovered the presence of five distinct profiles from a total of 430 yeast isolates. The five obtained strains were evaluated at microvinifications trials and tested for basic oenological and biochemical parameters including sulphur dioxide and ethanol tolerance as well as H2S production in sterile grape must. The selected autochthonous yeast strains named, Soi2 (Agiorgitiko wine) and L2M (Moschofilero wine), were evaluated also in industrial (4000L) fermentations to assess their sensorial and oenological characteristics. The volatile compounds of the produced wines were determined by GC-FID. Our results demonstrated the feasibility of using Soi2 and L2M strains in industrial fermentations for Agiorgitiko and Moschofilero grape musts, respectively.

Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 201-210 ◽  
Author(s):  
F. N. Wachira ◽  
R. Waugh ◽  
W. Powell ◽  
C. A. Hackett
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Southeast Asia ◽  
Camellia Sinensis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Systematic Assessment ◽  
Tree Crop ◽  
Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

scholarly journals Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

2016 ◽  
Vol 97 (6) ◽  
pp. 1307-1315 ◽  
Author(s):  
Elangovan Dilipan ◽  
Jutta Papenbrock ◽  
Thirunavakkarasu Thangaradjou
Keyword(s):  
Genetic Variability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Seagrass Species ◽  
Complex Species ◽  
Marine Angiosperms ◽  
Different Populations ◽  
High Degree ◽  
Identification Tool

In India 14 seagrass species can be found with monospecific genera (Enhalus, ThalassiaandSyringodium),Cymodoceawith two species andHalophilaandHalodulerepresented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of theHalophilaandHalodulecomplex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.

scholarly journals The use of random amplified polymorphic DNA to evaluate the genetic variability of Ponkan mandarin (Citrus reticulata Blanco) accessions

2000 ◽  
Vol 23 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Helvécio Della Coletta Filho ◽  
Marcos Antonio Machado ◽  
M. Luiza P.N. Targon ◽  
Jorgino Pompeu Jr.
Keyword(s):  
Genetic Variability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Point Mutations ◽  
The Other ◽  
Citrus Reticulata ◽  
Random Primers ◽  
Citrus Reticulata Blanco ◽  
Ponkan Mandarin

RAPD analysis of 19 Ponkan mandarin accessions was performed using 25 random primers. Of 112 amplification products selected, only 32 were polymorphic across five accessions. The absence of genetic variability among the other 14 accessions suggested that they were either clonal propagations with different local names, or that they had undetectable genetic variability, such as point mutations which cannot be detected by RAPD.

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