scholarly journals Polymorphisms in coding and non-coding regions of rabbit (Oryctolagus cuniculus) myogenin (MyoG) gene

2021 ◽  
Vol 29 (2) ◽  
pp. 69
Author(s):  
Łukasz Migdał ◽  
Sylwia Pałka
Keyword(s):  
Association Analysis ◽  
Growth Traits ◽  
Silent Mutation ◽  
Upstream Sequence ◽  
Nucleotide Polymorphisms ◽  
Weaning Weight ◽  
Conserved Sequence ◽  
Longissimus Lumborum ◽  
Exon 1 ◽  
L Value

In animal breeding, selection based on growth is very often used, as this trait affects the profitability of animal production. Identification of  polymorphisms within the genes affecting the growth process seems to be very important. Therefore, we decided to analyse rabbit myogenin (<em>MyoG</em> gene) for potential polymorphic sites and their association with growth and carcass traits in Termond White (TER), Belgian Giant Grey (BGG) and crossbred New Zealand White×Belgian Giant Grey (NZW×BGG) rabbits. We found three single nucleotide polymorphisms (SNPs) – in 5’ upstream sequence g.68679476 C&gt;T, in exon 1 – silent mutation g.68680096 T&gt;C and g.68680097 G&gt;A resulting in change of GTG triplet (valine) into ATG triplet (methionine). Association analysis showed that GG genotype weaning weight was statistically higher compared to GA in TER population (<em>P</em>=0.005), and that the hind parts for GG genotypes were heavier compared to those of GA (<em>P</em>=0.024), but association analysis of dissectible parts showed this was caused by higher bone weight (<em>P</em>=0.015). For g.68679476 C&gt;T in NZW×BGG population, the CC genotypes for fore (678±35) and hind part (615±29) weights were heavier compared to CT (588±16 and 549±13, respectively); moreover, association analysis of dissectible parts showed that weight of dissectible meat in hind part. Unfortunately, we did not find similar associations for other analysed breeds. For g.68679476 C&gt;T in NZWxBGG musculus longissimus lumborum pH leg after 24 h chilling (pH24L) were statistically lower for CC genotypes compared to CT (<em>P</em>=0.027). For g.68680097 G&gt;A in Termond White population L* value on the hind leg after 24 h chilling (L*24H) was higher for GA genotypes compared to GG (<em>P</em>=0.03), while for g.68679476 C&gt;T for musculus longissimus lumborum L* value after 24 h (L*24L) CC genotypes had higher value compared to CT (<em>P</em>=0.016) in BGG population. Moreover, in BGG population CT genotypes had higher weaning weight compared to CC (<em>P</em>=0.018). Our results show that SNPs within the <em>MyoG </em>gene may influence growth traits in some rabbit breeds, but the evolutionary conserved sequence may not be favourable for changes within coding sequences. For a better understanding thereof, additional analysis is required.

Assessment of polymorphisms in mysostatin gene and their allele substitution effects showed weak association with growth traits in Iranian Markhoz goats

2016 ◽  
Vol 155 (3) ◽  
pp. 519-526 ◽  
Author(s):  
K. KHANI ◽  
A. ABDOLMOHAMMADI ◽  
S. FOROUTANIFAR ◽  
A. ZEBARJADI
Keyword(s):  
Growth Traits ◽  
Average Daily Gain ◽  
Substitution Effects ◽  
Potential Candidate ◽  
Nucleotide Polymorphisms ◽  
Economic Traits ◽  
Gene Effects ◽  
Mstn Gene ◽  
Goat Population ◽  
Exon 1

SUMMARYPolymorphisms in the myostatin (MSTN) gene were detected in 150 female Iranian Markhoz goats. Two 573 base pairs (bp) and 475 bp fragments of the MSTN gene, which contains a deletion 5 bp indel (206 TTTTA/), in the region of exon 1 encoding the 5′ untranslated region (UTR) of the MSTN transcript, and two single-nucleotide polymorphisms (SNPs) of substitution (339T/A, 169T/G) in exon 1 and 3 regions, respectively, were amplified. The polymerase chain reaction (PCR) products were digested separately using restriction enzyme endonuclease DraI, HinIII and HindIII. The digestion results indicated AA and AB genotypes in the region of exon 1 encoding the 5′UTR of the MSTN transcript, AA, AT and TT genotypes in exon 1 and TT, TG and GG genotypes in exon 3. The SNPs loci were in Hardy–Weinberg disequilibrium but the deletion locus showed equilibrium in the Markhoz goat population. Evaluation of associations between the polymorphisms with the studied growth traits showed that the AA and GG genotypes of exons 1 and 3 have a significant positive effect on weight at 6 months of age (W6) and average daily gain (ADG) traits, but genotypes in the region of exon 1 encoding the 5′UTR of the MSTN transcript did not have any significant effect on the studied growth traits. The statistical analyses showed a positive and significant effect of the 339A allele (exon 1) for W6 and negative and significant effect of the 169G allele (exon 3) for ADG trait. Therefore, these results suggest that the MSTN gene could be a potential candidate gene that affects ADG and W6 traits in goats. More studies are needed to simultaneously consider variants of this region in a larger population to better understand MSTN gene effects on the economic traits in goat.

scholarly journals Function of the Porcine TRPC1 Gene in Myogenesis and Muscle Growth

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 147
Author(s):  
Yu Fu ◽  
Peng Shang ◽  
Bo Zhang ◽  
Xiaolong Tian ◽  
Ruixue Nie ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Growth Traits ◽  
Expression Profiles ◽  
Muscle Growth ◽  
Receptor Potential ◽  
Transient Receptor Potential Channel ◽  
Nucleotide Polymorphisms ◽  
Pig Breeds ◽  
Muscle Tissues ◽  
Transient Receptor

In animals, muscle growth is a quantitative trait controlled by multiple genes. Previously, we showed that the transient receptor potential channel 1 (TRPC1) gene was differentially expressed in muscle tissues between pig breeds with divergent growth traits base on RNA-seq. Here, we characterized TRPC1 expression profiles in different tissues and pig breeds and showed that TRPC1 was highly expressed in the muscle. We found two single nucleotide polymorphisms (SNPs) (C-1763T and C-1604T) in TRPC1 that could affect the promoter region activity and regulate pig growth rate. Functionally, we used RNAi and overexpression to illustrate that TRPC1 promotes myoblast proliferation, migration, differentiation, fusion, and muscle hypertrophy while inhibiting muscle degradation. These processes may be mediated by the activation of Wnt signaling pathways. Altogether, our results revealed that TRPC1 might promote muscle growth and development and plays a key role in Wnt-mediated myogenesis.

Variability of the IGF2 locus in the Suino Nero Lucano pig population and its effects on meat quality

2018 ◽  
Vol 58 (11) ◽  
pp. 1976 ◽  
Author(s):  
Amalia Simonetti ◽  
Andrea Rando ◽  
Paola Di Gregorio ◽  
Carmelisa Valluzzi ◽  
Annamaria Perna ◽  
...  
Keyword(s):  
Meat Quality ◽  
Shear Force ◽  
Quality Characteristics ◽  
Porcine Insulin ◽  
Drip Loss ◽  
Fat Percentage ◽  
Promoter Regions ◽  
Igf2 Gene ◽  
Longissimus Lumborum ◽  
L Value

The aim of this study was to analyse the polymorphisms in the two promoter regions, P1 and P2, of the porcine Insulin-like Growth Factor 2 (IGF2) gene and to investigate the effect of IGF2 genotypes on meat quality traits in the Italian autochthonous Suino Nero Lucano pig. Three polymorphic sites were analysed and only two of the eight potential haplotypes were observed in the Suino Nero Lucano pig population: A haplotype (–366A – –225G – –182C), and B haplotype (–366G – –225C – –182T). Muscle mass and meat quality characteristics were analysed in 30 castrated pigs (10 for each of the three IGF2 genotypes: A/A, A/B, and B/B). According to the results, B/B animals, at the same carcass weight, showed the highest Longissimus lumborum and Psoas weight (P < 0.05), whereas A/A animals showed a higher intramuscular fat percentage and lower Warner–Bratzler shear force, drip loss, and polyunsaturated fatty acids content. Meat from B/B animals showed also a higher L* value and myoglobin and deoxymyoglobin percentage compared with meat from A/A ones (P < 0.05).

scholarly journals Evaluación fenotípica y genética para características de crecimiento en la raza criolla colombiana Costeño con Cuernos

2007 ◽  
Vol 7 (2) ◽  
pp. 12 ◽  
Author(s):  
Rodrigo Alfredo Martínez ◽  
Juan Esteban Pérez ◽  
Teófilo Herazo
Keyword(s):  
Birth Weight ◽  
Variance Components ◽  
Fixed Effects ◽  
Genetic Parameters ◽  
Growth Traits ◽  
Phenotypic Correlation ◽  
Phenotypic Variance ◽  
Direct Effects ◽  
Weaning Weight ◽  
Number Of Births

<p>Se establecieron componentes de varianza, así como parámetros fenotípicos y genéticos, respecto de las variables ‘peso al nacimiento’, ‘peso al destete’ (ajustado a los 270 días) y ‘peso a los 480 días’ en un hato del ganado criollo colombiano Costeño con Cuernos. Se analizaron 2.281 registros de pesos al nacer, 1.722 de pesos al destete  y 1.086 de pesos ajustados a los 480 días utilizando la metodología de máxima verosimilitud restringida (DFREML). También se ajustó un modelo animal que incluyó efectos genéticos directos, maternos y de ambiente permanente, asumiendo como efectos fijos el año de nacimiento, el sexo del ternero y el número de partos de la madre; finalmente, se estimaron los parámetros genéticos ‘heredabilidad’, ‘repetibilildad’ y se establecieron correlaciones genéticas y fenotípicas. Se reportan bajas estimaciones de heredabilidad de los efectos directos, que varían entre 0,17 ± 0,001 y 0,21 ± 0,074 para los pesos al nacer y al destete, respectivamente; así mismo, fue baja la heredabilidad de los efectos genéticos maternos con relación al peso al nacimiento, aunque estos estimados aumentaron respecto de los pesos al nacer y al destete. Las correlaciones entre efectos directos y maternos fueron negativas, pero el mayor valor se encontró para el peso al nacimiento (-0,89). La contribución del ambiente permanente como proporción de la varianza fenotípica total fue baja y disminuyó a medida que aumentó la edad del animal.</p><p> </p><p><strong>Genetic and phenotypic evaluation to characterize growth traits of the native Colombian breed Costeño con Cuernos</strong></p><p>For a herd of native Colombian breed of cattle -Costeño con Cuernos (CCC)- estimates of variance components for phenotypic and genetic parameters were obtained for birth weight, weight at weaning (adjusted to 270 days) and weight at 480 days. Using the restricted maximum likelihood (REMI) methodology, 2281 birth weight records (PN), 1722 weaning weight records and 1086 weight records adjusted to 480 days were analyze by fitting a model which included direct and maternal genetics effects as well as permanent environmental effects, assuming that fixed effects were year of birth weight, calf gender and the mother number of births. The genetic parameters for heritability, repeatability, genetic and phenotypic correlation were estimated and genotypic and phenotypic correlation was established. Heritability estimates for direct effects are low and range from 0.17 ± 0.001 and 0.21 ± 0.074 for birth and weaning weight respectively; while estimates for maternal genetics effects were also low for PN, they were higher for weaning weight and weight at 480 days. There was a negative correlation between direct and maternal effects, and the higher value was for PN (-0.89). The contribution of the variable permanent environment measured as the contribution of the phenotypic variance was low and diminished as animal age increased.</p>

scholarly journals Prevalence, Patterns, and Genetic Association Analysis of Modic Vertebral Endplate Changes

2017 ◽  
Vol 11 (4) ◽  
pp. 594-600 ◽  
Author(s):  
Rishi Mugesh Kanna ◽  
Rajasekaran Shanmuganathan ◽  
Veera Ranjani Rajagopalan ◽  
Senthil Natesan ◽  
Raveendran Muthuraja ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Genetic Association ◽  
Association Analysis ◽  
Disc Degeneration ◽  
Lumbar Disc ◽  
Nucleotide Polymorphisms ◽  
Lumbar Disc Degeneration ◽  
Vertebral Endplate ◽  
Genetic Association Analysis ◽  
Study Population

<sec><title>Study Design</title><p>A prospective genetic association study.</p></sec><sec><title>Purpose</title><p>The etiology of Modic changes (MCs) is unclear. Recently, the role of genetic factors in the etiology of MCs has been evaluated. However, studies with a larger patient subset are lacking, and candidate genes involved in other disc degeneration phenotypes have not been evaluated. We studied the prevalence of MCs and genetic association of 41 candidate genes in a large Indian cohort.</p></sec><sec><title>Overview of Literature</title><p>MCs are vertebral endplate signal changes predominantly observed in the lumbar spine. A significant association between MCs and lumbar disc degeneration and nonspecific low back pain has been described, with the etiopathogenesis implicating various mechanical, infective, and biochemical factors.</p></sec><sec><title>Methods</title><p>We studied 809 patients using 1.5-T magnetic resonance imaging to determine the prevalence, patterns, distribution, and type of lumbar MCs. Genetic association analysis of 71 single nucleotide polymorphisms (SNPs) of 41 candidate genes was performed based on the presence or absence of MCs. SNPs were genotyped using the Sequenome platform, and an association test was performed using PLINK software.</p></sec><sec><title>Results</title><p>The mean age of the study population (n=809) was 36.7±10.8 years. Based on the presence of MCs, the cohort was divided into 702 controls and 107 cases (prevalence, 13%). MCs were more commonly present in the lower (149/251, 59.4%) than in the upper (102/251, 40.6%) endplates. L4–5 endplates were the most commonly affected levels (30.7%). Type 2 MCs were the most commonly observed pattern (n=206, 82%). The rs2228570 SNP of VDR (<italic>p</italic>=0.02) and rs17099008 SNP of MMP20 (<italic>p</italic>=0.03) were significantly associated with MCs.</p></sec><sec><title>Conclusions</title><p>Genetic polymorphisms of SNPs of VDR and MMP20 were significantly associated with MCs. Understanding the etiopathogenetic mechanisms of MCs is important for planning preventive and therapeutic strategies.</p></sec>

WEIGHTED AVERAGES OF SIRE PROGENY TESTS FOR GROWTH TRAITS IN RECORD OF PERFORMANCE BEEF HERDS

1981 ◽  
Vol 61 (1) ◽  
pp. 35-44 ◽  
Author(s):  
L. R. SCHAEFFER ◽  
J. A. ERIKSSON ◽  
J. W. WILTON
Keyword(s):  
Growth Traits ◽  
Monitoring Program ◽  
Weighted Average ◽  
Test Program ◽  
Weaning Weight ◽  
Declining Enrollment ◽  
Beef Herds ◽  
Progeny Tests ◽  
Annual Trends ◽  
Genetic Trends

Growth records of 281 744 calves born from 1971 to 1978 were used to calculate annual phenotypic and genetic trends for weaning weight and yearling weight. Genetic trends were computed as the weighted average of sire-transmitting abilities obtained from the Record of Performance National Beef Sire Monitoring Program. There were no significant differences between the genetic trends of all calves vs. calves which made a yearling weight for either weaning weight or yearling weight. Annual trends were positive for Angus, Hereford, and Shorthorn breeds, and were negative for Charolais, Limousin, Maine-Anjou and Simmental breeds. The interpretation of annual trends was complicated by the declining enrollment of herds on the test program which could have artificially altered the averages.

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