scholarly journals Gene Therapy in Periodontal Tissue Engineering

2016 ◽  
Vol 8 (1) ◽  
pp. 46-48
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Gene Delivery ◽  
Periodontal Regeneration ◽  
Periodontal Tissue ◽  
Delivery Method ◽  
Molecular Events ◽  
Artificial Extracellular Matrix ◽  
Periodontal Healing ◽  
Primary Strategy

ABSTRACT An elaborate system of signaling molecules regulates the cellular and molecular events of periodontal healing, the primary strategy for which is functional periodontal compartment regeneration and replication of components of the natural cellular microenvironment by providing an artificial extracellular matrix and by delivering growth factors. A new, so-called gene delivery method works by converting cells into protein- producing factories, thereby bypassing the dilemma. Gene therapy can channel the cellular signals in a controlled and very systematic manner, to provide encoded proteins at every stage of tissue regeneration. The aim of this review is to highlight the applications of gene delivery and tissue engineering in periodontal regeneration. How to cite this article Lakhani N, Vandana KL. Gene Therapy in Periodontal Tissue Engineering. CODS J Dent 2016;8(1):46-48.

scholarly journals Gene Therapy of Bone Morphogenetic Protein for Periodontal Tissue Engineering

2003 ◽  
Vol 74 (2) ◽  
pp. 202-213 ◽  
Author(s):  
Q-M. Jin ◽  
O. Anusaksathien ◽  
S.A. Webb ◽  
R.B. Rutherford ◽  
W.V. Giannobile
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Morphogenetic Protein ◽  
Periodontal Tissue ◽  
Morphogenetic Protein

scholarly journals Platelet-Derived Growth Factor (PDGF) Gene Delivery for Application in Periodontal Tissue Engineering

2001 ◽  
Vol 72 (6) ◽  
pp. 815-823 ◽  
Author(s):  
William V. Giannobile ◽  
Caroline S. Lee ◽  
Matthew P. Tomala ◽  
Kristina M. Tejeda ◽  
Zhimin Zhu
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Gene Delivery ◽  
Platelet Derived Growth Factor ◽  
Periodontal Tissue

Gene Delivery for Periodontal Tissue Engineering: Current Knowledge – Future Possibilities

2009 ◽  
Vol 9 (4) ◽  
pp. 248-266 ◽  
Author(s):  
Fa-Ming Chen ◽  
Zhi-Wei Ma ◽  
Qin-Tao Wang ◽  
Zhi-Fen Wu
Keyword(s):  
Tissue Engineering ◽  
Gene Delivery ◽  
Current Knowledge ◽  
Periodontal Tissue

Physiological features of periodontal regeneration and approaches for periodontal tissue engineering utilizing periodontal ligament cells

2007 ◽  
Vol 103 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Bruno Braga Benatti ◽  
Karina Gonzales Silvério ◽  
Márcio Zaffalon Casati ◽  
Enílson Antônio Sallum ◽  
Francisco Humberto Nociti
Keyword(s):  
Tissue Engineering ◽  
Periodontal Ligament ◽  
Periodontal Regeneration ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue

Periodontal Tissue Engineering with a Multiphasic Construct and Cell Sheets

2019 ◽  
Vol 98 (6) ◽  
pp. 673-681 ◽  
Author(s):  
C. Vaquette ◽  
S. Saifzadeh ◽  
A. Farag ◽  
D.W. Hutmacher ◽  
S. Ivanovski
Keyword(s):  
Tissue Engineering ◽  
Periodontal Ligament ◽  
Cell Sheet ◽  
Periodontal Regeneration ◽  
Surrounding Tissue ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Micro Computed Tomography ◽  
Cell Sheets ◽  
Tissue Integration

This study reports on scaffold-based periodontal tissue engineering in a large preclinical animal model. A biphasic scaffold consisting of bone and periodontal ligament compartments manufactured by melt and solution electrospinning, respectively, was used for the delivery of in vitro matured cell sheets from 3 sources: gingival cells (GCs), bone marrow–derived mesenchymal stromal cells (Bm-MSCs), and periodontal ligament cells (PDLCs). The construct featured a 3-dimensional fibrous bone compartment with macroscopic pore size, while the periodontal compartment consisted of a flexible porous membrane for cell sheet delivery. The regenerative performance of the constructs was radiographically and histologically assessed in surgically created periodontal defects in sheep following 5 and 10 wk of healing. Histologic observation demonstrated that the constructs maintained their shape and volume throughout the entirety of the in vivo study and were well integrated with the surrounding tissue. There was also excellent tissue integration between the bone and periodontal ligament compartments as well as the tooth root interface, enabling the attachment of periodontal ligament fibers into newly formed cementum and bone. Bone coverage along the root surface increased between weeks 5 and 10 in the Bm-MSC and PDLC groups. At week 10, the micro–computed tomography results showed that the PDLC group had greater bone fill as compared with the empty scaffold, while the GC group had less bone than the 3 other groups (control, Bm-MSC, and PDLC). Periodontal regeneration, as measured by histologically verified new bone and cementum formation with obliquely inserted periodontal ligament fibers, increased between 5 and 10 wk for the empty, Bm-MSC, and PDLC groups, while the GC group was inferior to the Bm-MSC and PDLC groups at 10 wk. This study demonstrates that periodontal regeneration can be achieved via the utilization of a multiphasic construct, with Bm-MSCs and PDLCs obtaining superior results as compared with GC-derived cell sheets.

scholarly journals Periodontal Destruction and Regeneration in Experimental Models: Combined Research Approaches

2020 ◽  
Vol 5 (5) ◽  
pp. 28-34
Author(s):  
Olena J. Kordiyak ◽  
Keyword(s):  
Tissue Engineering ◽  
Animal Models ◽  
Periodontal Disease ◽  
Periodontal Ligament ◽  
Periodontal Regeneration ◽  
Sufficient Evidence ◽  
Computer Assisted ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Computed Tomography Scanning

Chronic periodontitis is a common dental disease, resulting in destruction of gingival tissue, periodontal ligament, cementum, alveolar bone and, consequently- teeth loss in the adult population. Experimental animal models have enabled the study of periodontal disease pathogenesis and are used to test new therapeutic approaches for treating the disease The purpose of this review study was to draw the evidence from animal models, required for future assessment of destructional and regenerative processes in periodontal tissues. Material and methods: a rat experimental periodontitis models of ligature, streptozotocin, and immune complexes induced periodontitis, periodontal defect, altered functional loading, stress exposures and surgically created chronic acid reflux esophagitis models. Histomorphomorphological/-metrical, immunohisto (-cyto)chemical and histopathological analysis, micro-computed tomography, scanning and transmission electron microscopy, polarizing light and confocal microscopy, spectrophotometry, radiographic and biomechanical analysis, descriptive histology and computer-assisted image analysis. Results and discussion. Scaling and root planing may not always be effective in preventing periodontal disease progression, and, moreover, with currently available therapies, full regeneration of lost periodontal tissues after periodontitis cannot be achieved. However, in 70.5% of the results of experimental studies reported, irrespective of the defect type and animal model used, beneficial outcome for periodontal regeneration after periodontal ligament stem cell implantation, including new bone, new cementum and new connective tissue formation, was recorded. Therefore, platelet-rich fibrin combined with rat periodontal ligament stem cells provides a useful instrument for periodontal tissue engineering. Conclusion. There is sufficient evidence from preclinical animal studies suggesting that periodontal tissue engineering would provide a valuable tool for periodontal regeneration. Further elaboration of the developed in preclinical studies experimental techniques should justify progress to clinical studies and subsequent medical application

Future of Periodontal Regeneration

2010 ◽  
Vol 4 (Spl) ◽  
pp. 38-47 ◽  
Author(s):  
Ranjan Malhotra ◽  
Anoop Kapoor ◽  
Vishakha Grover ◽  
Nitin Verma ◽  
Jasjit Kaur Sahota
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Rna Interference ◽  
Human Genetics ◽  
Current Knowledge ◽  
Periodontal Regeneration ◽  
Therapeutic Approaches ◽  
Polypeptide Growth Factors ◽  
Complicating Factors ◽  
Therapeutic Measures

ABSTRACT The management of periodontal defects has been an ongoing challenge in clinical periodontics. In the recent past, attention has been focused more on regenerative and reconstructive therapies i.e. bone grafts, guided tissue regeneration, root conditioning, polypeptide growth factors, rather than on respective therapies. These therapeutic measures are shown to be limited in the predictability of healing and regenerative response in the modern clinical practice because oral environment presents several complicating factors that border regeneration. The 21st century appears to represent a time in history when there is a convergence between clinical dentistry and medicine, human genetics, developmental and molecular biology, biotechnology, bioengineering, and bioinformatics, resulting in the emergence of novel regenerative therapeutic approaches viz. tissue engineering, gene therapy and RNA interference. The focus of this review paper is to furnish and update the current knowledge of periodontal tissue engineering, gene therapy and RNA interference i.e. the future of periodontal regeneration.

scholarly journals Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

2020 ◽  
Vol 11 ◽  
pp. 508-532 ◽  
Author(s):  
Varsha Sharma ◽  
Anandhakumar Sundaramurthy
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Gene Therapy ◽  
Gene Delivery ◽  
Driving Forces ◽  
Capsule Formation ◽  
Potential Applications ◽  
Weak Polyelectrolytes ◽  
External Stimuli ◽  
Selection Of

Multilayer capsules have been of great interest for scientists and medical communities in multidisciplinary fields of research, such as drug delivery, sensing, biomedicine, theranostics and gene therapy. The most essential attributes of a drug delivery system are considered to be multi-functionality and stimuli responsiveness against a range of external and internal stimuli. Apart from the highly explored strong polyelectrolytes, weak polyelectrolytes offer great versatility with a highly controllable architecture, unique stimuli responsiveness and easy tuning of the properties for intracellular delivery of cargo. This review describes the progress in the preparation, functionalization and applications of capsules made of weak polyelectrolytes or their combination with biopolymers. The selection of a sacrificial template for capsule formation, the driving forces involved, the encapsulation of a variety of cargo and release based on different internal and external stimuli have also been addressed. We describe recent perspectives and obstacles of weak polyelectrolyte/biopolymer systems in applications such as therapeutics, biosensing, bioimaging, bioreactors, vaccination, tissue engineering and gene delivery. This review gives an emerging outlook on the advantages and unique responsiveness of weak polyelectrolyte based systems that can enable their widespread use in potential applications.

Study of a new nano-hydroxyapatite/basic fibroblast growth factor composite promoting periodontal tissue regeneration

2020 ◽  
Vol 10 (11) ◽  
pp. 1802-1807
Author(s):  
Haiying Wang ◽  
Yanmin Wu ◽  
Zhengyu Yao ◽  
Cong Wang
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Fibroblast Growth ◽  
Blank Group ◽  
Tissue Scaffold

Tissue engineering technology provides a new method for periodontal regeneration. Finding or preparing a suitable scaffold is the key to periodontal tissue engineering. Here, we use nano-hydroxyapatite-modified collagen biomimetic material (nHAC) as the packaging material, and carry fibroblast growth factor (bFGF) for the regeneration and repair of periodontal tissue. Due to its low cytotoxicity and high biocompatibility, nHAC shows unique advantages in the construction of periodontal tissue scaffolds. The nHAC periodontal tissue scaffold material has a dense and porous three-dimensional network structure, has a high loading rate of bFGF, and can firmly lock human periodontal ligament cells (HPDLCs), which is easy for cell growth and attachment. In vivo experiments have shown that, in artificial animal periodontal tissue models, the nHAC-loaded bFGF periodontal scaffold covered by Geistlich Bio-Gide (GBG) membrane is better than the simple GBG membrane and the blank group, the nHAC/bFGF-GBG composite membrane It is beneficial to promote the growth of new alveolar bone and cement formation, and realize the regeneration of periodontal tissue.

scholarly journals Gene Delivery Therapeutics in the Treatment of Periodontitis and Peri-Implantitis: A State of the Art Review

2019 ◽  
Vol 20 (14) ◽  
pp. 3551 ◽  
Author(s):  
Funda Goker ◽  
Lena Larsson ◽  
Massimo Del Fabbro ◽  
Farah Asa’ad
Keyword(s):  
Gene Therapy ◽  
Regenerative Medicine ◽  
Periodontal Disease ◽  
Viral Vectors ◽  
Periodontal Regeneration ◽  
Normal Function ◽  
Periodontal Tissue ◽  
Chronic Inflammatory Condition

Background: Periodontal disease is a chronic inflammatory condition that affects supporting tissues around teeth, resulting in periodontal tissue breakdown. If left untreated, periodontal disease could have serious consequences; this condition is in fact considered as the primary cause of tooth loss. Being highly prevalent among adults, periodontal disease treatment is receiving increased attention from researchers and clinicians. When this condition occurs around dental implants, the disease is termed peri-implantitis. Periodontal regeneration aims at restoring the destroyed attachment apparatus, in order to improve tooth stability and thus reduce disease progression and subsequent periodontal tissue breakdown. Although many biomaterials have been developed to promote periodontal regeneration, they still have their own set of disadvantages. As a result, regenerative medicine has been employed in the periodontal field, not only to overcome the drawbacks of the conventional biomaterials but also to ensure more predictable regenerative outcomes with minimal complications. Regenerative medicine is considered a part of the research field called tissue engineering/regenerative medicine (TE/RM), a translational field combining cell therapy, biomaterial, biomedical engineering and genetics all with the aim to replace and restore tissues or organs to their normal function using in vitro models for in vivo regeneration. In a tissue, cells are responding to different micro-environmental cues and signaling molecules, these biological factors influence cell differentiation, migration and cell responses. A central part of TE/RM therapy is introducing drugs, genetic materials or proteins to induce specific cellular responses in the cells at the site of tissue repair in order to enhance and improve tissue regeneration. In this review, we present the state of art of gene therapy in the applications of periodontal tissue and peri-implant regeneration. Purpose: We aim herein to review the currently available methods for gene therapy, which include the utilization of viral/non-viral vectors and how they might serve as therapeutic potentials in regenerative medicine for periodontal and peri-implant tissues.

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