In vitro propagation of a rare and endangered species, Echinosophora koreensis Nakai, by axillary bud culture

AbstractGentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.

Download Full-text

Optimization of in vitro cultivation conditions for rare and endangered species of Berberis iliensis and Berberis karkaralensis

BULLETIN of the L N Gumilyov Eurasian National University BIOSCIENCE Series ◽

10.32523/2616-7034-2020-132-3-43-56 ◽

2020 ◽

Vol 132 (3) ◽

pp. 43-56

Author(s):

V.K. Karimova ◽

◽

B.N. Baktybai ◽

G.K. Magzumova ◽

ZH. T. Sartbaev ◽

...

Keyword(s):

Endangered Species ◽

Nutrient Medium ◽

Anthropogenic Activities ◽

In Vitro Cultivation ◽

Cultivation Conditions ◽

Rare And Endangered Species ◽

Optimization Study ◽

Rare And Endangered ◽

Living Organisms

Today, many living organisms are negatively affected by climate change and anthropogenic activities, which leads to a decrease in their numbers. One of these rare and endangered plant species is the Ili barberry (Berberis iliensis) and the Karkaraly barberry (Berberis karkaralensis). This work is devoted to the optimization study of the cultivation conditions for a rare and endangered species of Ili barberry and Karkaralinsky barberry in vitro. To obtain sterile and viable explants, the sterilizing agent was a solution of 0.5% «Domestos» (7 min). For the regeneration of the main shoot of the barberry, the optimal nutrient medium is Murashige and Skoogwith the addition of 6-benzylaminopurine- 0.5 mg/l, gibberellic acid- 1.0 mg/l, indole-3-butyric acid -0.01 mg/l, where regeneration was 80% for the Ili barberry, barberry karkaralinsky - 70%. For the multiplication of Berberis iliensis microshoots, the Quoirin & Lepoivre culture medium with the addition of 0.75 mg/l - benzylaminopurine is optimal; the number of microshoots formed was 3.6 per explant. Root formation is one of the most difficult stages in micropropagation. For the rooting of microshoots of Karkaralinsky barberry, a nutrient medium of ½ Murashige and Skoog was used with the addition of indolylbutyric acid -1.5 mg/l.

Download Full-text

Peculiarities of morphogenesis of the endangered species of wilow (Salix spp.) in vitro

Biologija ◽

10.6001/biologija.v65i1.3986 ◽

2019 ◽

Vol 65 (1) ◽

Cited By ~ 1

Author(s):

Oksana Chornobrov ◽

Svitlana Bilous ◽

Oleksandr Chornobrov ◽

Maria Manko

Keyword(s):

Endangered Species ◽

Culture Medium ◽

Rare And Endangered Species ◽

Stepwise Method ◽

The Status ◽

Mass Reproduction ◽

Rare And Endangered ◽

Data Book ◽

Salix Spp

Conservation and reproduction of rare genotypes of Salix L. species, in particular the blunt-leaved willow (Salix retusa L.) and Jacquin’s Willow (Salix alpina Scop.) that are listed in the Red Data Book of Ukraine in the status of rare and endangered species, is one of the urgent tasks of the present. The aim of research was to develop methods of introduction of S. retusa and S. alpina into in vitro culture for their mass reproduction and conservation. The plant material was cultivated on a culture medium prescribed by MS, WPM, and DKW with the addition of growth regulators according to the conventional method. Effective sterilization (over 80%) of explants of S. retusa and S. alpina was achieved by applying a stepwise method, which consisted of consistently maintaining them in solutions 0.1% HgCl2 and 1.0% AgNO3 for 5–6 min. Significant results in the regeneration of explants by activating the growth of available meristems in vitro were observed on MS with the addition of 0.25–0.5 mg/l 6-(Furfurylaminopurine, kinetin) and 2 g/l activated carbon. Our further research will serve as a base for developing microclonal propagation of S. retusa and S. alpina for their conservation and reproduction in vitro.

Download Full-text

Effect of different light sources and ventilation on in vitro shoot growth and rooting of a rare and endangered species, Tsuru-rindo(Tripterospermum japonicum)

Journal of Plant Biotechnology ◽

10.5010/jpb.2008.35.3.215 ◽

2008 ◽

Vol 35 (3) ◽

pp. 215-221 ◽

Cited By ~ 5

Author(s):

Heung-Kyu Moon ◽

So-Young Park

Keyword(s):

Endangered Species ◽

Shoot Growth ◽

Light Sources ◽

Rare And Endangered Species ◽

Rare And Endangered

Download Full-text

ИЗОГЕНЕЗ ЕКСПЛАНТІВ SILENE HYPANICA KLOKOV ТА ЇХ АДАПТАЦІЯ ДО УМОВ EX VITRO

Scientific Issue Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: Biology ◽

10.25128/2078-2357.20.1-2.6 ◽

2020 ◽

Vol 79 (1-2) ◽

pp. 48-53

Author(s):

L. L. Dzhus ◽

L. A. Koldar

Keyword(s):

Endangered Species ◽

Red List ◽

Ex Vitro ◽

Active Process ◽

Berne Convention ◽

Nutrient Media ◽

Important Place ◽

Rare And Endangered Species ◽

Rare And Endangered

Among the conservation measures related to the preservation of plant diversity, an important place belongs to the creation and preservation of rare and endangered species in the crop conditions. Thus, the species Silene hypanica Klokov is under threat of extinction. This relict and endemic species is listed in the Red book of Ukraine, the European Red list, and 6th Resolution of the Berne Convention. Prospects for the conservation of this species are related to the study of its viability and require the development of effective methods of reproduction, in particular in vitro crop. The article presents the results of studies of one of the breeding stages in vitro – rhizogenesis of S. hypanica esplants and its dependence on the concentrations of β-indolyl butyric acid in nutrient media (0,1; 0,5; 1,0; 1,5; 2,0 mg / l), followed by adaptation of regenerating plants to ex vitro conditions. The study showed that the active process of rhizogenesis occurred due to the addition of β-IMC to the nutrient medium at a concentration of 0.5 mg/l and contributed to the production of 86.4 % of rooted regenerating plants. Transplanting rooted plants into peat disks and then growing them in containers contributed to the successful adaptation of rooted plants.

Download Full-text

Genetic diversity and genetic structure of the rare and endangered species, Primula ranunculoides

Biodiversity Science ◽

10.3724/sp.j.1003.2013.09098 ◽

2014 ◽

Vol 21 (5) ◽

pp. 601-609

Author(s):

Wang Deyun ◽

Peng Jie ◽

Chen Yajing ◽

Lü Guosheng ◽

Zhang Xiaoping ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Endangered Species ◽

Rare And Endangered Species ◽

Rare And Endangered

Download Full-text

Geoecological analysis of the Russian Federation regions representation in the state program “Environmental Protection”

Geodesy and Cartography ◽

10.22389/0016-7126-2020-957-3-47-53 ◽

2020 ◽

Vol 957 (3) ◽

pp. 47-53

Author(s):

E.A. Kravets

Keyword(s):

Endangered Species ◽

Environmental Protection ◽

Russian Federation ◽

The State ◽

Volga River ◽

Unequal Distribution ◽

State Program ◽

Rare And Endangered Species ◽

Rare And Endangered ◽

The Russian Federation

The author offers mapping and geoecological analysis of the Russian Federation regions presence in the state program “Environmental Protection”. The unequal distribution of the program’s targets and activities in different regions is revealed. A considerable number of relevant environmental problems for several mentioned regions have not been reflected in the program. It is important to increase the area of specially protected natural areas for a significant number of subjects of the Russian Federation. The status “part of the territory occupied by specially protected natural territories of Federal value in the total area of the subject of the Russian Federation” is recommended to be assigned all regions of Russia. Identification and elimination of objects of accumulated environmental damage that threat to the Volga river is relevant, at least for all the regions in which the Volga flows. Not all regions with a high level of air pollution and/or large masses of air pollutants have the indicator “reduction of total emissions for the reporting year”. It is necessary to increase the Program of measures for the protection of rare and endangered species of plants and to expand the list of regions in which it is planned to protect rare and endangered species of animals.

Download Full-text

Research on a Biodiversity Conservation Value Assessment Method Based on Habitat Suitability of Species: A Case Study in Gansu Province, China

Sustainability ◽

10.3390/su13063007 ◽

2021 ◽

Vol 13 (6) ◽

pp. 3007

Author(s):

Xiaojiong Zhao ◽

Jian Wang ◽

Junde Su ◽

Wei Sun ◽

Haoxian Meng

Keyword(s):

Ecosystem Services ◽

Endangered Species ◽

Biodiversity Conservation ◽

Gansu Province ◽

Distribution Area ◽

Suitable Habitat ◽

Conservation Value ◽

Value Assessment ◽

Rare And Endangered Species ◽

Rare And Endangered

Quantitative assessment and evaluation of ecological parameters and biodiversity conservation are prime concerns for long-term conservation of rare and endangered species and their associated habitats in any ecological region. In this study, Gansu Province, a biodiversity hotspot, was chosen as the research area. We predicted the distribution patterns of suitable habitats for rare and endangered species. The replacement cost method was adopted to calculate the conservation value of rare and endangered species. The suitable habitat distribution area of rare and endangered wild animals reached 351,607.76 km2 (without overlapping area), while that of plants reached 72,988.12 km2 (without overlapping area). The conservation value of rare and endangered wildlife is US $1670.00 million. The high-value areas are mostly concentrated in the south and north of Gansu Province. The conservation value of rare and endangered wild plants is US $56,920.00 million. The high-value areas are mostly concentrated south of Gansu Province. The conservation value is US $58,590.00 million a year, and its distribution trend is gradually decreasing from northeast to southwest, with the highest in the forest area south of Gansu Province, followed by the Qilian Mountain area in the north. These results are of great significance for future improvement of the evaluation index system of ecosystem services and the development of ecosystem services and management strategies.

Download Full-text