Avaliação antimicrobiana dos extratos vegetais das folhas da espécie Mangífera indica Linnaeus

2021 ◽  
Author(s):  
BIANCA FERREIRA BORGES ◽  
ALANA DA CONCEIçãO BRITO COELHO ◽  
DANIELLE ANDREA PEREIRA COZZANI CAMPOS ◽  
JEOVANA THAYNARA OLIVEIRA MARTINS ◽  
PALOMA SAMPAIO DA COSTA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Mangifera Indica

Desde os tempos mais remotos o ser humano vem fazendo uso de plantas medicinais para cura de seus males espirituais e físicos. Porém, o uso dessas plantas era feito de maneira empírica onde o conhecimento foi sendo construindo de maneira experimental e observada, passando de pais para filhos. Contudo, estudos científicos tornaram seu consumo ainda mais popular e o os tratamentos a base de plantas medicinais vêm se tornando cada vez mais comum. E para que os constituintes químicos de um vegetal possa ser registrado, é necessário a realização de testes que comprovam a ausência ou presença de composto com atividades biológicas benéficas ou maléficas ao homem. O presente trabalho buscou avaliar a atividade antimicrobiana dos extratos vegetais da espécie Mangifera indica Linnaeus, onde foram utilizadas cepas padrão provenientes da American Type Culture Colection (ATCC), sendo bactérias Gram-positivas: Staphylococcus aureus (ATCC 25923) e Streptococcus pyogenes (ATCC 19615); e bactérias Gram-negativas: Escherichia coli (ATCC 25922) e Pseudomonas aeruginosa (ATCC 27853). Apresentou inibição satisfatória para todas as frações referente a cepa Staphylococcus aureus (ATCC 25923), apresentando resultados variáveis para as demais cepas estudadas, onde algumas frações não possuíram carácter inibitório. A variação de diâmetro dos halos de inibição esteve entre 5-30 mm.,

scholarly journals Microorganisms Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria

2019 ◽  
pp. 1-8
Author(s):  
O. G. Palmer ◽  
A. K. Onifade
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Pyogenes ◽  
Bacterial Isolates ◽  
Environmental Surfaces ◽  
Ondo State

Nosocomial infection is a rising problem in developing countries. Microorganisms Isolated from three private and public hospital environmental surfaces in Akure Metropolis, Ondo State, Nigeria were investigated in this study. Bacterial and fungal organisms were isolated and compared among the three hospitals. The study revealed that bacteria were the most predominant microorganisms found in the hospital environmental surfaces than fungi. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolates while fungi include Aspergillus fumigatus, Aspergillus niger and Candida albicans.  Staphylococcus aureus was found to be predominant bacteria but Aspergillus funmigatus was the predominant fungi. The result showed that the microbial loads of the public hospitals were higher than that of the private hospitals. The bacteria load of the male ward was found to be higher than that of the female ward while the fungal loads of each of the hospital environmental surfaces of female were higher than that of the male. The study revealed that bacteria were the most predominant microorganisms found in the hospital environment than fungi. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolates while fungi include Aspergillus fumigatus, Aspergillus niger and Candida albicans.  Staphylococcus aureus was found to be predominant bacteria. All the hospital environmental surfaces were contaminated with one or more microorganisms in the course of the research.

scholarly journals Eficiência de sabonetes comerciais antissépticos contra bactérias patogênicas e análise dos rótulos

2021 ◽  
Vol 10 (2) ◽  
pp. e20310212241
Author(s):  
Jeisa Patricia Leitzke ◽  
Tatiany Alves Nierri ◽  
Jaqueline Hoscheid ◽  
Nathielle Miranda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Pyogenes ◽  
Staphylococcus Epidermidis

Os sabonetes em barra com ação antisséptica são destinados à limpeza de resíduos presentes no corpo, sua utilização tem crescido significativamente em decorrência da preocupação da população com determinadas doenças causadas por contaminação microbiológica. Com isso, têm surgido no mercado diferentes marcas de sabonetes garantindo a eliminação de 99,9% das bactérias. O objetivo deste estudo foi realizar a análise comparativa do pH, atividade antibacteriana e rótulos de 5 diferentes marcas de sabonetes antibacterianos em barra, identificados como A, B, C D e E. Realizou-se a determinação do pH com auxílio de pHmetro digital, e na análise microbiológica utilizou-se a metodologia de disco difusão, com o intuito de observar a presença ou ausência da multiplicação das bactérias Streptococcus pyogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus e Escherichia coli frente aos diferentes sabonetes testados. Durante a análise da rotulagem dos sabonetes foi avaliada a presença do requisito “composição”, exigido pela resolução vigente.  Nos testes realizados constatou-se que o pH de todas as marcas testadas encontraram-se fora dos parâmetros estabelecidos. Na análise microbiológica observou-se que apenas a marca E, demonstrou efeito antisséptico frente às bactérias testadas. Os demais sabonetes apresentaram menor e até mesmo nenhum potencial de inibição do crescimento desses microrganismos. Os produtos analisados neste estudo apresentam em suas formulações duas substâncias ativas, o triclosan e o triclocarban, porém os mesmos podem ser nocivos para a saúde humana e meio ambiente, e induzirem a resistência bacteriana, por isso, alguns países já proibiram o uso destes ativos. Segundo os resultados obtidos é possível concluir que os rótulos presentes das embalagens destas diferentes marcas analisadas estão em desacordo com o estabelecido pelos fabricantes.

Structure of plantaricin locus of Lactobacillus plantarum 8P-A3

2011 ◽  
Vol 2 (4) ◽  
pp. 255-261 ◽  
Author(s):  
A. Tsapieva ◽  
N. Duplik ◽  
A. Suvorov
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Sequence Analysis ◽  
Lactobacillus Plantarum ◽  
Streptococcus Pyogenes ◽  
Antagonistic Activity ◽  
Wide Range ◽  
Encoding Genes

Lactobacillus plantarum 8P-A3 is a strain which is well known on the Russian pharmaceutical market and it is included in several probiotic products. The strain has been widely used since 1973 but the mechanisms of its antibacterial activity were unknown. L. plantarum 8P-A3 expressed high antagonistic activity against the wide range of bacterial pathogens including Streptococcus agalactiae, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae strains. DNA sequence analysis of L. plantarum 8P-A3 genome revealed the presence of a complete plantaricin locus of about 20,000 bp encoding genes of at least two bacteriocins – plantaricins EF and NC8. The plantaricin locus found in L. plantarum 8P-A3 is homologous to the plantaricin cluster in L. plantarum J51.

scholarly journals Time-kill kinetics and antibacterial activity of root extract of Adenodolichos paniculatus (Hua) Hutch & Dalz (Fabaceae)

2021 ◽  
Vol 18 (2) ◽  
pp. 95-102
Author(s):  
Friday I. Kyahar ◽  
Edith A. Onwuliri ◽  
Joseph O. Ehinmidu ◽  
Peters O. Oladosu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Antibacterial Activities ◽  
Root Extract ◽  
Log Reduction ◽  
Zones Of Inhibition ◽  
Time Kill

Medicinal plants have been used in treatment of illness from time immemorial. Adenodolichos paniculatus is a medicinal plant used for traditional remedy of sore throat infections. This study therefore, evaluated the antibacterial activities of the root extracts and time-kill kinetics of the most potent extract. Five extracts, obtained by maceration using n-hexane, chloroform, ethyl acetate, methanol and water sequentially were evaluated for antibacterial activities and time-kill kinetics against Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Chloroform root extract at 100 mg/ml was active against Streptococcus pyogenes, Staphylococcus aureus and Pseudomonas aeruginosa with zones of inhibition 25.00, 21.00 and 14.75 mm respectively but not against Escherichia coli. Minimum inhibitory concentrations were 1.56, 6.25 and 25.00 mg/ml respectively and the minimum bactericidal concentrations were 3.12, 12.50 and 50.00 mg/ml. Complete elimination of S. pyogenes, S. aureus and P. aeruginosa was achieved at concentrations 1.56 mg/ml, 6.25 mg/ml and 25.00 mg/ml within 300, 720 and 960 minutes exposure respectively and at concentrations 3.12 mg/ml, 25.00 mg/ml and 50.00 mg/ml within 180, 300 and 720 minutes exposure respectively. Chloroform root extract has the potential to be used as antibacterial agent and was better than the other solvent extract two-fold. Keywords: Adenodolichos paniculatus; Antibacterial activity; Time-kill kinetics; Percentage reduction, Log reduction

scholarly journals Bacteriological Analysis of Beef Production Chain in Birnin Kebbi Metropolis of Kebbi State, Nigeria

2020 ◽  
pp. 64-76
Author(s):  
Famubo, Joseph A. ◽  
Isiaka, Aishatu ◽  
Abbas, Yusuf B.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Micrococcus Luteus ◽  
Klebsiella Pneumonia ◽  
Proteus Vulgaris ◽  
Production Chain ◽  
Retail Outlets ◽  
Retail Meat

The hygiene status of most abattoirs and retail outlets in Nigeria is very poor, and it contributes to the unacceptable level of bacteria load in beef, and poses a health risk to consumers. This study examined the bacteriological analysis of beef production chain in Birnin Kebbi metropolis. A total of 100 samples of meat, water and surface swab in Main Market, GRA, Badariya, Bayan Kara and Rafin Atiku, Birnin Kebbi Central abattoir and retail meat shops were collected aseptically, processed and analyzed. Meat from retail outlets were mostly contaminated by Staphylococcus aureus (24.4%), followed by Escherichia coli (17.3%), Klebsiella pneumonia (9.8%), Salmonella typhimurium (9.1%), Entrobater aerogenes (8.5%), Streptococcus pyogenes (8.1%), Proteus vulgaris (7.8%), Micrococcus luteus (7.8%) and Pseudomonas aeruginosa (7.2%). In the abattoirs contact surfaces, contamination occurred mostly by Staphylococcus aureus (23.4%), Salmonella typhimurium (18.2%), Escherichia coli (16.9%), Entrobater aerogenes (9.1%), Streptococcus pyogenes (7.8%), Klebsiella pneumonia, Proteus vulgaris, and Pseudomonas aeruginosa (6.5% each) and Micrococcus luteus (5.2%). The findings revealed a high bacterial load in both abattoir and retail meat outlets. In order to safeguard the health of the public against the risks of food borne infections, there is a need to educate and advocate good sanitation and meat handling practices in the abattoir and beef retail outlets.

scholarly journals An Andrographolide from Helichrysum caespitium (DC.) Sond. Ex Harv., (Asteraceae) and Its Antimicrobial, Antiquorum Sensing, and Antibiofilm Potentials

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1224
Author(s):  
Kokoette Bassey ◽  
Patience Mamabolo ◽  
Sekelwa Cosa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Neisseria Gonorrhoeae ◽  
Streptococcus Pyogenes ◽  
Biofilm Formation ◽  
Cell Attachment ◽  
Chloroform Extract ◽  
Drug Candidate ◽  
Klebsiella Pneumonia

Helichrysum caespititium (DC.) Sond. Ex Harv., (Asteraceae) is a medicinal plant indigenous to South Africa. Its non-polar extracts exhibit significant antimicrobial and, in particular, antigonorrheal activity. This study aimed at isolating and purifying the active antigonorrheal compound from its chloroform extract and validating its inhibition potential on quorum sensing (QS) and biofilm formation of multi-drug resistant (MDR) pathogens. Phytochemical investigation of aerial parts of H. caespititium afforded a diterpene lactone (CF6). The effect of CF6 on violacein production and biofilm formation was studied using in vitro quantitative violacein inhibition (Chromobacterium violaceum) and biofilm formation (Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Neisseria gonorrhoeae, and Pseudomonas aeruginosa). The structure of CF6 was characterized using FTIR, NMR, and UPLC-MS data accordingly, as 10-methyl-8-(propan-17-ylidene)naphthalen-9-yl)-11-vinyl-14-hydroxyfuran-16-one. The susceptibility testing of the pathogens against CF6 revealed Neisseria gonorrhoeae was noticeably susceptible with a MIC value of 60 µg/mL, while Streptococcus pyogenes and Staphylococcus aureus showed MIC of 125 µg/mL. All gram-negative pathogens, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa were inhibited at 250 µg/mL. CF6 also inhibited the production of violacein by 51.88% at 250 µg/mL and prevented cell attachment by 40.76–81.18%, with N. gonorrhoeae being highly prohibited from forming biofilm. In conclusion, 10-methyl-8-(propan-17-ylidene)naphthalen-9-yl)-11-vinyl-14-hydroxyfuran-16-one is the first of its kind to be isolated from the non-polar (chloroform) extract of South African Helichrysum caespititium with antigonorrheal, antimicrobial, antiquorum sensing, and antibiofilm properties. The compound may serve as a drug candidate against MDR pathogens.

scholarly journals Antibacterial activity of methanol extracts of the leaves of three medicinal plants against selected bacteria isolated from wounds of lymphoedema patients

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dereje Nigussie ◽  
Gail Davey ◽  
Belete Adefris Legesse ◽  
Abebaw Fekadu ◽  
Eyasu Makonnen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Pyogenes ◽  
Bacterial Species ◽  
Wound Infections ◽  
Methanol Extracts

Abstract Background Patients with lymphoedema are at high risk of getting bacterial and fungal wound infections leading to acute inflammatory episodes associated with cellulitis and erysipelas. In Ethiopia, wound infections are traditionally treated with medicinal plants. Methods Agar well diffusion and colorimetric microdilution methods were used to determine the antibacterial activity of methanol extracts of the three medicinal plants against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shewanella alage, methicillin-resistant S. aureus ATCC®43300TM, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC700603, and Pseudomonas aeruginosa ATCC37853. Results The methanol extract of L. inermis leaves showed high activity against all tested bacterial species, which was comparable to the standard drugs. Similarly, the extracts of A. indica showed activity against all tested species though at higher concentrations, and higher activity was recorded against Streptococcus pyogenes isolates at all concentrations. However, the extract of A. aspera showed the lowest activity against all tested species except Streptococcus pyogenes isolates. The lowest minimum inhibitory concentration (MIC) was recorded with the extract of L. inermis against E. coli isolate and S. aureus ATCC 25923. Conclusion Methanol extracts of L. inermis, A. indica, and A. aspera leaves exhibited antimicrobial activity against selected bacterial isolates involved in wound infections, of which the methanol extracts of L. inermis exhibited the highest activity. The results of the present study support the traditional use of plants against microbial infections, which could potentially be exploited for the treatment of wound infections associated with lymphoedema.

scholarly journals The Immunomodulatory Activity in vitro of NDV ZG1999HDS in Comparison to NDV La Sota

2021 ◽  
Author(s):  
Bratko Filipič ◽  
Lidija Gradišnik ◽  
Adriana Pereyra ◽  
Hrvoje Mazija
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Gram Negative Bacteria ◽  
Acinetobacter Baumanii ◽  
Gram Negative ◽  
Gm Csf ◽  
Gram Positive Bacteria ◽  
Streptococcus Mutants

ABSTRACT. The immunotherapies, as a modern therapeutic approach, get an attention because of theirs’ promise to treat a large number of different medical disorders. Immunomodulation effects of low titres (10 HA/ml) of NDV (Newcastle Disease Virus) ZG1999HDS or La Sota were tested on TLT (Human macrophage cell line) bound to PBMC (Peripheral Blood Mononuclear Cells). During the immunomodulation, the amount of NO, H2O2, lysozym and induced antibacterial activity against Gram - positive bacteria (Staphylococcus aureus, MRSA, Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus mutants) and against Gram - negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Acinetobacter baumanii) were analysed. In addition, the cytokine secretion, IL-1α, IL-2, IL-4, GM-CSF, TNF-α, IFN-α and IFN-α were evaluated. Firstly, the TLT cells are activated through the NDV ZG1999HDS or La Sota binding, followed by the NO “burst” and H2O2 and lysozyme level increase. Secondly, after the binding to the TLT cells and interaction with the PBMCs, the decrease of GM-CSF, and an increase of TNF – α and IFN – γ were found. Simultaneously, the decrease of pro – inflammatory cytokine IFN-α and the differentially increase of IL-1α, IL-2 and IL-4 were recorded. During the induction of the antibacterial response, against Gram - positive bacteria (Staphylococcus aureus, MRSA, Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus mutants) the effect was one third higher with NDV ZG1999HDS compared to La Sota. Antibacterial response against Gram - negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Acinetobacter baumanii) was not so clear. In general, NDV ZG1999HDS or La Sota activated TLT cells, further bound to PBMC; the ZG1999HDS is stronger immunomodulator than La Sota.

scholarly journals МОНИТОРИНГ ВИДОВОГО СОСТАВА И АНТИБИОТИКОРЕЗИСТЕНТНОСТИ ВОЗБУДИТЕЛЕЙ УРОИНФЕКЦИЙ

2020 ◽  
pp. 20
Author(s):  
Е. П. Котелевец
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Streptococcus Pyogenes ◽  
Streptococcus Faecium ◽  
Klebsiella Spp

Цель. Мониторинг структуры и антимикробной резистентности возбудителей уроинфекций для повышения эффективности эмпирической терапии у пациентов Рязанской области. Исследовались штаммы бактерий, выделенные из мочи пациентов в возрасте от 18 до 68 лет с заболеваниями мочевыделительной системы, находящихся на лечении в урологических отделениях многопрофильных больниц г. Рязани в 2018 – 2019гг. Определялись видовая принадлежность выделенной чистой культуры и чувствительность к антибиотикам и антимикробным препаратам. При анализе видового состава возбудителей выявлено преобладание грамотрицательных бактерий (Escherichia coli, Klebsiella spp., Citrobacter spp.). Грамположительная флора была представлена бактериями родов Streptococcus, Enterococcus и Staphylococcus. При ретроспективном анализе антимикробной чувствительности Escherichia coli выявлена эффективность амикацина, цефтриаксона, гентамицина, цефоперазона и цефотаксима (97%, 95%, 92%, 92% и 92% протестированных изолятов соответственно); Streptococcus pyogenes – рифампицина и амоксиклава (96%), ципрофлоксацина и ампициллина (95%); Staphylococcus aureus – рифампицина (88% протестированных изолятов), ванкомицина и фузидина – 87% и 82% соответственно. Наибольшая чувствительность Enterococcus faecalis отмечалась к амоксиклаву – 96%, ципрофлоксацину -91%, ампициллину – 83% протестированных изолятов; Pseudomonas aeruginosa – к полимиксину (86%) и сизомицину (82%). В исследованных образцах доминировали представители семейства Enterobacteriaceae (69,17%) и Streptococcaceae (24,28%); внутри этих семейств – Escherichia coli (47,03%) и Streptococcus faecium (7,8%) соответственно. Наибольшая чувствительность Escherichia coli отмечалась к амикацину (97%), цефтриаксону (95%), к гентамицину, цефоперазону и цефотаксиму – у 92% протестированных изолятов; Streptococcus faecium – к рифампицину и амоксиклаву (96%), ципрофлоксацину и ампициллину (95%). Постоянный мониторинг микробного пейзажа мочи и чувствительности к антибиотикам и антимикробным препаратам может повысить эффективность эмпирической антибактериальной терапии при инфекции мочевыводящих путей.

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