scholarly journals SELECTION OF AUTOCHThONOUS sACCHAROMYCES AND NON-sACCHAROMYCES YEASTS STRAINS ACCORDING TO THEIR EXTRACELLULAR ENZIMATIC ACTIVITY

2020 ◽  
Vol 1 (1) ◽  
pp. 119-124
Author(s):  
Elena Brȋnduşe ◽  
Marian Ion ◽  
Cǎtǎlina Loredana Nedelcu ◽  
Lidia Fȋciu ◽  
Adrian Pantazi
Keyword(s):  
Protease Activity ◽  
Extracellular Enzymes ◽  
Secondary Compounds ◽  
Wine Production ◽  
Selective Media ◽  
Yeast Strains ◽  
Intense Activity ◽  
Saccharomyces Yeasts ◽  
Selection Of

The aim of the present study was to investigate the production of extracellular enzymes in a number of twenty six autochtonous Saccharomyces and nonSaccharomyces strains selected in Dealu Mare region for wine production. The strains were screened for the production of extracellular b-glucosidase, esterase, pectinase and protease activity by inoculation the yeast strains onto selective media. All Saccharomyces tested strains showed at least two enzymatic activities while nonSaccharomyces strains showed activity at least for one enzyme. The weakest activity was recorded in case of β-glucosidase. Most of the tested strains exhibit more or less intense activity for polygalacturonase/pectinase and protease. This study put into evidence the potential of autochtonous and especially of non-Saccharomyces strains as source of production of secondary compounds which can play an important role in improving the quality of wines.

scholarly journals Identification and selection of non-Saccharomyces strains isolate from brazilian apple must

2018 ◽  
Vol 48 (5) ◽  
Author(s):  
Giovana Arruda Moura Pietrowski ◽  
Juliana Vitória Messias Bittencourt ◽  
Luciana Rocha Brandão ◽  
Carlos Augusto Rosa ◽  
Aline Alberti ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Pichia Anomala ◽  
Pcr Analysis ◽  
Hanseniaspora Uvarum ◽  
Candida Oleophila ◽  
Aroma Production ◽  
Fuji Apple ◽  
Saccharomyces Yeasts ◽  
Selection Of

ABSTRACT: This study aimed to know the biodiversity of non-Saccharomyces yeasts in Brazilian apples must with potential to improve of the aromatic quality of ciders. The strains were isolated from thirty-five (35) Gala and Fuji apple musts from different locations from south region of Brazil. Forty-five (45) strains were isolate and identified by PCR analysis. Results indicated ten (10) species: Candida oleophila, Candida parapsilosis, Candida tropicalis, Clavispora lusitaniae, Hanseniaspora guilliermondii, Hanseniaspora uvarum, Lodderomyces elongisporus, Pichia anomala, Pichia fermentans and Rhodotorula mucilaginosa. The genus Rhodotorula sp., Lodderomyces sp. and Clavispora sp. constituted 71.2% of the strains identified. The following strains, C. oleophila, R. mucilaginosa, P. fermentans, H. uvarum and H. guilliermondii were selected in qualitative tests due the fruity aroma production by trained team in the aromatic assessment of cider.

scholarly journals Exploitation of technological variability among wild non-Saccharomyces yeasts to select mixed starters for the production of low alcohol wines

2019 ◽  
Vol 15 ◽  
pp. 02031 ◽  
Author(s):  
A. Grazia ◽  
A. Pietrafesa ◽  
A. Capece ◽  
R. Pietrafesa ◽  
G. Siesto ◽  
...  
Keyword(s):  
Sugar Content ◽  
Negative Influence ◽  
Wine Quality ◽  
Alcohol Content ◽  
Grape Must ◽  
Promising Strategy ◽  
Yeast Strains ◽  
Low Alcohol ◽  
Saccharomyces Yeasts

Increase of the sugar content in grape must, and consequently, alcohol levels in wine are some of the principal problems affecting the winemaking industry. High alcohol content can compromise wine quality, creating sensory imbalances, as well as decreasing the perception of some flavors. The technological approaches proposed at this aim, although allowing achievement of the purpose, can determine negative influence on quality of wine. A promising strategy is based on the use of specific microorganisms, such as selected yeast strains, mainly non-Saccharomyces, able to convert grape must sugars towards secondary metabolites rather than ethanol. This study aims at screening of wild non-Saccharomyces strains in order to identify those suitable for the use in mixed starter for the production of wine with reduced alcohol content and, at the same time, with improved aromatic characteristics.

INFLUENCE OF THE TYPE OF YEAST ON QUALITY OF GRAPE WINE MATERIAL

2018 ◽  
Author(s):  
П.А. ЧАЛДАЕВ ◽  
А.Г. КАШАЕВ ◽  
А.Е. ЛЕУЧЕВ ◽  
С.С. МАЛЫШКИН
Keyword(s):  
Wine Yeast ◽  
Volatile Components ◽  
Yeast Strains ◽  
Grape Wine ◽  
Fusel Oils ◽  
Key Indicators ◽  
And Storage ◽  
Selection Of ◽  
Direct Distillation

Рассмотрена возможность повышения качества виноматериалов с помощью подбора штамма винных дрожжей. Исследовано 12 штаммов дрожжей различных производителей и ценовых категорий. Виноградное сусло получали из белого винограда технического сорта Цитронный Магарача, выращенного в Самарской области. Качество винограда соответствовало требованиям ГОСТ 31782–2012. Переработку винограда вели в полупромышленных условиях по «белому» способу. Дозировка дрожжей составляла 0,2 г/л. Брожение сусла и хранение полученных виноматериалов осуществляли при температуре 14–15°С. Показатели качества полученных сухих столовых виноматериалов удовлетворяли требованиям ГОСТ 32030–2013 по всем основным показателям. Из виноматериалов путем прямой перегонки получены винные дистилляты и подвергнуты газохроматографическому анализу. По содержанию летучих компонентов – метанола, сивушных масел, сложных эфиров, ацетальдегида, бензальдегида, кетонов все образцы соответствовали требованиям ГОСТ 31493–2012. Установлено, что по скорости сбраживания, флокулирующей способности и продуцированию летучих компонентов наилучшим для выбранного сорта винограда является штамм дрожжей Vitilevure DV10 (Danstar Ferment AG, Дания). Possibility of improvement of quality of wine materials by means of selection of a necessary strain of wine yeast is considered. 12 yeast strains of different producers and price categories were investigated. The grape mash was received from white grapes of a technical grade Tsitronnyy Magaracha who is grown up in the Samara region. The quality of grapes conformed to requirements of GOST 31782–2012. Processing of grapes was conducted in semi-industrial conditions on a white way. The dosage of yeast was 0,2 g/l. Fermentation of a mash and storage of the received wine materials carried out at a temperature of 14–15°C. Indicators of quality of the received dry table wine materials met requirements of GOST 32030–2013 for all key indicators of quality. From wine materials by direct distillation wine distillates was obtained and subjected to gas chromatographic analysis. According to the content of volatile components-methanol, fusel oils, esters, acetaldehyde, benzaldehyde, ketones, all samples met the requirements of GOST 31493–2012. It was found that the rate of fermentation, flocculating ability and production of volatile components is best for the selected grape variety strain Vitilevure DV10 (Danstar Ferment AG, Denmark).

scholarly journals The effect of yeast strains on the formation of aroma complex of base wines from 'Tsitronnyi Magaracha' grape variety

2021 ◽  
pp. 66-71
Author(s):  
Максим Юрьевич Шаламитский ◽  
Татьяна Николаевна Танащук ◽  
Виктор Афанасьевич Загоруйко ◽  
София Николаевна Червяк
Keyword(s):  
Yeast Species ◽  
Chemical Compounds ◽  
Higher Alcohols ◽  
Grape Variety ◽  
Organoleptic Evaluation ◽  
Wine Production ◽  
Terpene Alcohols ◽  
Yeast Strains ◽  
Grape Varieties ◽  
Selection Of

Одним из важных современных направлений виноделия является получение высококачественных вин с узнаваемой сортовой индивидуальностью. В данном аспекте наиболее привлекательными для потребителя являются вина из мускатных сортов винограда. Pабота посвящена изучению влияния штаммов дрожжей рода Saccharomyces на формирование сортового аромата виноматериалов из винограда сорта Цитронный Магарача и отбору перспективных штаммов для их производства. Объектами исследования являлись виноматериалы, приготовленные с использованием 20 штаммов дрожжей вида S. cerevisiae. В результате хроматографического анализа было идентифицировано 17 компонентов, относящихся к различным группам химических соединений: высшим спиртам, сложным эфирам, терпеновым спиртам. Исследуемые штаммы способствовали накоплению в виноматериалах терпеновых спиртов в концентрации от 0,46 до 1,51 мг/л, высших спиртов - от 153,86 до 263,89 мг/л, сложных эфиров - от 3,96 до 19,09 мг/л. По результатам органолептической оценки опытных образцов для производства вин из винограда сорта Цитронный Магарача рекомендованы два коллекционных штамма Алиготе М (I-76) и Мускат 4 (Р) (I-637) из КМВ «Магарач». Использование данных штаммов позволило получить виноматериалы с ярким развитым ароматом цветочно-фруктового направления с проявлением тонов цитрусовых и розы. Отмечено, что штамм Алиготе М (I-76) способствовал обогащению виноматериалов терпеновыми спиртами и сложными эфирами, штамм Мускат 4 (Р) (I-637) - сложными эфирами. One of the important modern trends in winemaking is the production of high quality wines with recognizable varietal identity. In this aspect, the most attractive for consumers are the wines from muscat grape varieties. The work is devoted to the study of the effect of yeast strains of the Saccharomyces genus on the formation of varietal aroma of base wines from grapes of 'Tsitronnyi Magaracha' variety and selection of promising strains for their production. The objects of the study were base wines prepared using 20 strains of S. cerevisiae yeast species. As a result of chromatographic analysis, 17 components of various groups of chemical compounds were identified: higher alcohols, esters, terpene alcohols. The studied strains contributed to the accumulation of terpene alcohols in concentration from 0.46 to 1.51 mg/l, higher alcohols - from 153.86 to 263.89 mg/l, esters - from 3.96 to 19.09 mg/l. According to the results of organoleptic evaluation of experimental samples for wine production from grapes of 'Tsitronnyi Magaracha' variety, two strains 'Aligote M' (I-76) and 'Muscat 4' (P) (I-637) from the Magarach Collection of Microorganisms of Winemaking are recommended. The use of these strains have made it possible to obtain base wines with advanced rich aroma of a floral-fruity direction with demonstration of citrus and rose hues. It was noted that the strain 'Aligote M' (I-76) contributed to the enrichment of base wines with terpene alcohols and esters, the strain 'Muscat 4' (P) (I-637) - with esters.

Autolytic capacity and foam analysis as additional criteria for the selection of yeast strains for sparkling wine production

2001 ◽  
Vol 18 (2) ◽  
pp. 183-191 ◽  
Author(s):  
Adolfo Martı́nez-Rodrı́guez ◽  
Alfonso V. Carrascosa ◽  
José M. Barcenilla ◽  
M. Angeles Pozo-Bayón ◽  
M. Carmen Polo
Keyword(s):  
Sparkling Wine ◽  
Wine Production ◽  
Yeast Strains ◽  
Selection Of

scholarly journals Wine Saccharomyces Yeasts for Beer Fermentation

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 290
Author(s):  
Vanesa Postigo ◽  
Margarita García ◽  
Juan Mariano Cabellos ◽  
Teresa Arroyo
Keyword(s):  
Higher Alcohols ◽  
Beer Fermentation ◽  
Commercial Strain ◽  
Yeast Strains ◽  
Consumer Study ◽  
Apparent Attenuation ◽  
Melatonin Production ◽  
Craft Beers ◽  
Saccharomyces Yeasts ◽  
Selection Of

Multiple studies in recent years have shown the potential of Saccharomyces wild yeasts to produce craft beers with new flavour profiles and other desirable properties. Yeasts isolated from food (wine, bread, kombucha…) have shown potential promise for application in brewing. The aim of this study is to evaluate the ability of 141 Saccharomyces yeast strains isolated from the Madrilenian agriculture (from grapes, must, wine, vineyard, and cellars) to produce a novel ale beer. Fermentation activity of the strains was compared against the commercial strain Saccharomyces cerevisiae Safale S-04. In addition to the other aspects such as melatonin production, thirty-three volatile compounds belonging to higher alcohols, esters, aldehydes/cetones, acids, lactones and phenolic groups, were analysed by GC for selection of the strains. Ten strains were finally chosen, among which the most relevant was the strain G 520 showing a higher production of esters, higher alcohols and acids compared with S-04. The apparent attenuation for this strain was lower than commercial strain, which translates into more residual sugars. Furthermore, G 520 was more capable of producing significantly higher amounts of melatonin studied by HPLC, as well as showing a higher antioxidant capacity. Consumer study showed that G 520 strain could be used to produce a potential beer that has a place in the current market.

scholarly journals Contribution of Non-Saccharomyces Yeasts to Wine Freshness. A Review

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 34 ◽  
Author(s):  
Antonio Morata ◽  
Carlos Escott ◽  
María Bañuelos ◽  
Iris Loira ◽  
Juan del Fresno ◽  
...  
Keyword(s):  
Emerging Technologies ◽  
Yeast Strains ◽  
The Right ◽  
Saccharomyces Yeasts ◽  
Selection Of

Freshness, although it is a concept difficult to define in wines, can be understood as a combination of different circumstances. Organolepticwise, bluish red, floral and fruity, more acidic and full-bodied wines, are perceived as younger and fresher by consumers. In traditional winemaking processes, these attributes are hard to boost if no other technology or biotechnology is involved. In this regard, the right selection of yeast strains plays an important role in meeting these parameters and obtaining wines with fresher profiles. Another approach in getting fresh wines is through the use of novel non-thermal technologies during winemaking. Herein, the contributions of non-Saccharomyces yeasts and emerging technologies to these parameters are reviewed and discussed.

scholarly journals KEBERADAAN SALMONELLA SP. PADA SUSU OLAHAN ASAL KEDAI SUSU DI SEKITAR PERMUKIMAN MAHASISWA INSTITUT PERTANIAN BOGOR

2020 ◽  
Vol 8 (1) ◽  
pp. 34-42
Author(s):  
Ulfa Apriliana Annisa ◽  
Mirnawati B Sudarwanto ◽  
Susi Soviana ◽  
Herwin Pisestyani
Keyword(s):  
Healthy Food ◽  
National Standard ◽  
Serological Tests ◽  
Selective Media ◽  
Settlement Area ◽  
Milk Samples ◽  
Negative Results ◽  
Selection Of

Nowadays, milk is one of the favorite drinks for Indonesian people. This relates to the awareness of society about healthy food is increasing. At the same time, there are many milk retail that are appearing. The purpose of this study was to detect the presence and level of contamination of Salmonella sp. in processed milk from milk retail around the students settlement area of ​​IPB University. In Indonesia the standard for identifying Salmonella sp. refer to Indonesian National Standard 2897: 2008. This method consists of 5 stages: pre-enrichment, enrichment, selective media planting, confirmation based on biochemical and serological tests. Milk samples in this study came from 14 milk retail in the students settlement area of the IPB University. The milk samples were taken are plain milk (milk that has not added flavor (flavorings), sugar, and ice). The selection of milk retail is taken within a radius of 2 km from the midpoint of the IPB campus in the Dramaga and Cilibende areas. Based on the results of this study, it was found that all milk samples examined showed negative results which means no Salmonella sp. contamination was found. From this study it can be concluded that the quality of processed milk sold in milk retail around the student settlement area of IPB University is good.

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