LAPORAN KASUS : PENANGANAN OBSTRUKSI USUS PADA ANJING DI BALI VETERINARY CLINIC

A local breed dog was brought to the veterinary clinic with signs of vomiting and decreased appetite. Physical examination revealed a pale mucosa of the eye and nose, 2 seconds of CRT, abdomen distention and stiffness. A haematology examination indicated WBC drop into 4.9, lymphocyte into 5.4, MCV to into 55.42 and Plate Distribusi Wide 8.1. The X-ray test showed the presence of an unknown mass around the intestine. Therefore, the dog was then diagnosed with intestinal obstruction. A laparotomy exploration with an enterotomy technique was performed to treat the condition. The surgery procedure found a mass of stone-like faeces that also consist of a mix of grass. The stone-like faeces were then removed from the intestine. Post-surgery monitoring was performed routinely and the dog received Cefotaxime Sodium (Claforan®) 22 mg/kg, Odansentron HCl (Zofran®) 0.2 mg/kg, Sucralfate (Carafate®) 0.8 gram, Lactulose (Cephulac®) 25 mL, Dexamethasone (Dexasone®) 1 mg/kg, Diphenhydramine HCl (Benadryl®) 3mg/kg, along with antiseptic treatment for the surgical incision. 3 days after the operation the dog began to show signs of recovery where the dog appeared to start moving and showed the desire to eat food. This condition can be prevented by not giving the dog food that bones. Lastly, the dog should be only released in a safe and knowledgeable environment.

STUDI KASUS : FIBROSARCOMA PADA ANJING POMERANIAN MIX

Fibrosarcoma is a malignant neoplasm of fibroblast that commonly found in middle-aged or older dogs. Breed and sex do not influence the incidence of fibrosarcoma. A 10 years old grey female mix Pomeranian with a bodyweight of 9.2 kg showed a semi-solid mass, with meat-like consistency that felt integrated with the tissue underneath. A mass with a diameter of ± 5 cm had been found in the left thigh near the anus. The other two unusual masses with smaller size (diameter ±1 cm) were also found at lateral sinister near extremities cranial of the body. A serial of diagnostic check-ups, such as physical and clinical check-up, USG, haematology, cytology, and histopathology tests was run to examine the dog condition. The USG result showed hypoechoic masses with slightly anechoic appearances. The haematology analysis showed a decrease in lymphocyte, MCV and hematocrit, meanwhile the granulocyte, MCHC and MHC showed an increasing trend. The cytology test revealed a fat-like vacuolization. Furthermore, the histology examination indicating the presence of large hyperchromic oval cells. The histopathology examination also found fibroblast cells that suspected as tumour cells with mitotic and infiltrated oval cores. Thus, the dog was diagnosed with fibrosarcoma and the prognosis was dubious. The surgery was done to remove the tumour masses.

AKTIVITAS LARVA Culex sp TERHADAP EKSTRAK SERAI DAPUR (Cymbopogon citratus) DI KECAMATAN MAULAFA KOTA KUPANG

Dengue hemorrhagic fever (DBD) is still a health problem in Kota Kupang.DBD controls have been continuously performed to reduce the population of Culex sp. However, the modern larvicide has caused an environmental problem, toxic to species non-target and found to be resistance. The present study aimed to investigate the larvicidal activity of Cymbopogon citratuson Culex sp. The extract of C. citratus was used for larvicidal activity at concentration of 0%; 0.2%; 0.4%; 0.6%; 0.8% and 1%. The mortality rate was calculated after 2, 4, 12 and 24 hours. The results showed that the mortality of larvae was 5.3% (0%); 45.3% (0.2%); 70.6% 0.4%; 96% (0.6%); 100% (0.8% and 1%). The present investigation suggests the possible use of C. citratus as an ideal ecofriendly, larvicidal agent for the control of Culex sp.

Profil Eosinofil Sapi Sumba Ongole yang Diberikan Pakan Tepung Kelor (Moringa oleifera Lam.)

Moringa oleifera Lam contains protein compounds, vitamin A, minerals, essential amino acids, antioxidants, and flavonoids, and has an impact on the hematological profile. Based on the evaluated phytochemical, The aim of this study was to identify the eosinophil image of Sumba Onggle Cattle fed with moringa. The research was started from March to September 2017. The research was carried out was in the Integrated Dry Land Laboratory, Nusa Cendana University. and Regional Health Laboratory of East Nusa Tenggara Province. The stages of the research were started with the selection of the sample of the studied cattle (12 adult Sumba Ongole Cattle), the adaptation period, and the duration of treatment of moringa on the cattle for 50 days. The research sample chosen was. The results of the research data showed that the value of eosinophil in the first treatment from 0.32 x 103 / µl to 0.47 x 103 / µl. In contrast to treatment 2 which has a range of eosinophils at a value of 0.04 x 103 / µl to 0.69 x 103 / µl. However, the eosinophil value at the end of the treatment was in the normal range in cattle (0.1 x 103 / µl-1,2x 103 / µl). Eosinophil value data on day 50 showed that eosinophil values tended to increase and in the normal range of eosinophils. These results indicate that the cows fed with moringa showed an eosinophil profile that remained in the normal range.

Pengaruh Pakan terhadap Status Fisiologi Kambing Kacang (Capra aegragus hircus) dengan Pola Pemeliharaan Insentif di Daerah Lahan Kering

The aim of this research was to determine the ratio between the provision of Moringa to the physiological status (body temperature) of female goats. This research was conducted in the dryland field laboratory of the Nusa Cendana University, using 16 adult female goats, with a range of 6-7 months and body weight ranging from 10-12 kg. Goats were divided into 2 groups, namely groups using ammoniated grass and concentrate formulations combined with Moringa and without Moringa. The results showed that the body temperature of kacang goats that placed in individual cages with incentive maintenance patterns with cage adaptation for approximately 45 days, with a feeding pattern with no moringa concentrate and with moringa that given in the morning ranged from 38.8-39.0˚C and 38.7-39.2˚C. While in the afternoon, ranging from 38.7-39.2˚C and 38.7-39.0˚C. The conclusion is this body temperature was still in the normal range of body temperature for young female kacang goats.

Efek Pemberian Kelor (Moringa oleifera Lam.) pada Pakan terhadap Gambaran Hemoglobin Sapi Ongole

The Miracle Tree has been known as a plant that contains complete nutrition which has anti-anaemia properties. This study aims to study the effect of adding Moringa to feed on the image of blood haemoglobin in cattle, especially Sumba Ongole cattle. The study was conducted in 2017, at the Integrated Dryland Laboratory of the University of Nusa Cendana and the Health Laboratory of the East Nusa Tenggara Province Health Service. The sample in this study were 12 Sumba Ongole cattle aged 2-3 years with a bodyweight of 210.5 kg- 249 kg. Treatments on cattle were divided into 4 groups. The results of the study showed that the haemoglobin level on day 0 of each treatment was still within normal limits. On days 25 and 50, each treatment was in the normal range of haemoglobin levels. Based on the results of the study, it was concluded that statistically giving Moringa flour to the feed had a significant effect on the haemoglobin levels of Ongole cattle.

Amplifikasi DNA Kandidat Gen Kuda Pacu Sumba

The Sumba horse is one of the local horses in Indonesia which is known as racing horse Several candidate genes are known to influence the outward characteristics of the Sumba racehorse, which play main role in the development of the horse's muscles from embryo to adulthood. This research aims to identify candidate genes for the Sumba racehorse in stallion and mares. Blood samples from 5 stallions and 5 mares were collected and analyzed. The method used in this research was by using polymerase chain reaction (PCR), electrophoresis and DNA sequencing. The results of DNA amplification fragments at a temperature of 600c showed a fragment size of 463 bp. A total of 10 samples were sequenced on the PCR machine. The forward primer was 5'-TATTCTTCTTGGGAGGGAGGACTACT-3 'and reverse primer was 5'-GCAAGTAATTAGCACAAAAATTTGAATG-3'. The obtained data was analyzed using the Basic Local Alignment Sealing Tool (BLAST). Result of this study could be used as an initial identification of candidate genes for racing activity in stallion and mares that can complement the selection of racing horses.

PENGARUH PEMBERIAN EKSTRAK DAUN KELOR (Moringa oleifera Lamk) TERHADAP KUALITAS MIKROBIOLOGI DAN ORGANOLEPTIK DAGING SAPI

Beef is one type of meat that is quite popular with almost all Indonesian people, especially in East Nusa Tenggara (NTT). Storage of beef at room temperature and open space can accelerate the occurrence of decay in meat. This is because the complete nutritional content and high water content in meat can be a good medium for the growth of pathogenic bacteria or spoilage bacteria. One of the efforts that can be done to prevent meat rot is to do a natural preservative method by utilizing plant parts that contain antimicrobial compounds. Moringa leaves are one part of the plant which is known to have antimicrobial compounds. This study aims to determine the effect of Moringa oleifera Lamk leaf extract on the microbiological and organoleptic quality of beef. This research is an experimental laboratory research. A total of 48 thigh beef (Biceps femoris) beef samples were tested in this study using a completely randomized factorial pattern design, namely concentration factors of 0% (K0), 5% (K1), 10% (K2), and 15% (K3) extract Moringa leaf and long storage factor at room temperature are 0 hours, 6 hours, 12 hours, and 18 hours and repeated three times. The parameters tested were color quality, texture, aroma, initial decay test, pH test, and total plate count (TPC) test. The results showed changes in the color, aroma, and texture of the meat. The Eber test shows the K3 group can last up to 18 hours. There was a very significant difference between the concentration of Moringa leaf extract on the length of storage of meat and the pH value of meat (P<0,01). Moringa leaf extract concentration factors and meat storage duration significantly influence the TPC value (P<0,01). The TPC value in the K3 group is below the SNI contamination limit for storage room temperature less than 18 hours.

PENGARUH INFUSA DAUN KELOR (Moringa oleifera Lamk) TERHADAP PERTUMBUHAN MIKROBIOLOGI DAN ORGANOLEPTIK DAGING BABI GILING SEGAR

Moringa oleifera Lamk is a shrub with a height of 7-11 m and thrives from the lowlands to an altitude of 700 m above sea level. Moringa can grow in tropical and subtropical areas on all types of soil and is resistant to dry spells for 6 months. Its high nutritional value, properties and benefits have earned Moringa the nickname Mother's Best friend and the Miracle Tree. In addition, moringa plants also have benefits as antioxidants and antimicrobials so that they can be used as preservatives. This study aims to determine the benefits of adding Moringa oleifera Lamk leaf infusion to the quality of pork minced meat.This research is an experimental laboratory research. The samples used in this study were 48 samples of ground thigh pork (biceps femoris), and this study used a fully randomized design factor pattern.The quality parameters of the meat samples examined are color, the smell, texture, pH, Postma test and Total Plate Count (TPC). The results showed that the addition of moring leaf infusion changed color, aroma an d eczema. The Postma test shows that the K3 group can last up to 6 hours. The TPC value in the K3 group is below the SNI contamination limit for 6 hours.

EFFECT OF LACTIC ACID PALM LACTIC BACTERIA ON SILAGE QUALITY

Kualitas silase dipengaruhi berbagai faktor, di antaranya bakteri asam laktat sebagai starter dalam proses fermentasi. Tujuan penelitian ini adalah untuk mengetahui pengaruh isolat bakteri asam laktat dari nira lontar dapat digunakan sebagai starter kualitas silase. Metode yang digunakan dalam penelitian ini adalah pembuatan probiotik, pembuatan silase dan pengujian kualitas silase. Penelitian ini dilakukan dalam 5 perlakuan yang diulang sebanyak 3 kali dan menggunakan bahan utama pembuatan silase yaitu seluruh tanaman jagung. Data yang diperoleh dianalisis secara deskriptif dan disajikan dalam bentuk gambar. Hasil penelitian menunjukkan bahwa silase berwarna hijau kecokelatan, memiliki aroma segar, adanya kerusakan silase kurang dari 1%, dan terjadi penurunan derajat keasaman (pH) pada perlakuan. Berdasarkan hasil penelitian dapat disimpulkan bakteri asam laktat dari nira lontar yang digunakan sebagai starter memberikan efek yang baik terhadap kualitas silase jagung.