Antioxidant, anti-cholinesterase, anti-α-glucosidase and prebiotic properties of beta-glucan extracted from Algerian barley

Beta-glucan, such as barley-derived beta-glucan (BBG), are homopolysaccharides that have attracted attention by their nutritional and therapeutic properties. The aim of this study was to evaluate the antioxidant power of BBG extracted from local Algerian variety of barley (SAIDA 183), and its acetylcholinesterase, alpha glucosidase inhibitory activity as well as its prebiotic potential by fermentation with lactic acid bacteria isolated from camel’s milk, namely lactococcuslactisssplactis (Lc.l.l) and leuconostocmesenteroidesspmesenteroides (Ln.m.m). The results revealed that BBG exhibited low activity against DPPH and ferric-reducing power (IC50 4018.61 ± 656.69 and A0.5 at 359.88 ±63.64 µg/mL respectively), in contrast to other antioxidant tests (ABTS, Beta-carotene and CUPRAC) where BBG demonstrated a moderate activity (IC50 529.91 ±26.37, IC50 161.013±13.322, A0.5 529.79 ± 48.65 µg/mL). The scavenging ability of hydroxyl radical and superoxide radical by BBG with an IC50 at 2268.38±101.57 µg/mL and IC50 345.26± 62.32 µg/mL, respectively, while enzymatic inhibition by BBG exhibited for AChE at IC50 859.164 ±64.46 μg/mL , BChE at IC50 at 725.470 ±30.95 , α-Amylase inhibitory activity at IC50 2986.785 ± 37.046 . The bacterial growth of the two strains used in this study is favorably affected by the use of BBG as the only carbon source, in comparison with glucose as a control. In light of these findings, it can be concluded that BBG have shown moderate antioxidant and enzyme inhibitory activities and can be used as a prebiotic by acting synergistically with probiotics in functional food matrices.

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Bioactive Compounds Isolated from Marine Bacterium Vibrio neocaledonicus and Their Enzyme Inhibitory Activities

Marine Drugs ◽

10.3390/md17070401 ◽

2019 ◽

Vol 17 (7) ◽

pp. 401 ◽

Cited By ~ 5

Author(s):

Isabel Gómez-Betancur ◽

Jianping Zhao ◽

Lin Tan ◽

Chang Chen ◽

Ge Yu ◽

...

Keyword(s):

Inhibitory Activity ◽

Marine Bacterium ◽

Biological Activities ◽

Moderate Activity ◽

Strong Activity ◽

Weak Activity ◽

Bothrops Asper ◽

Inhibitory Activities ◽

Alpha Glucosidase

Marine organisms are recognized as a source of compounds with interesting biological activities. Vibrio neocaledonicus has been reported on for its high effectiveness against corrosion in metals but it has been little studied for its chemical and biological activities. In this study, four compounds were isolated from V. neocaledonicus: indole (1); 1H-indole-3-carboxaldehyde (2); 4-hydroxybenzaldehyde (3) and Cyclo (-Pro-Tyr) (4); using a bioassay-guided method, since in a previous study it was found that the ethyl acetate extract was active on the enzymes acetylcholinesterase (AChE), alpha-glucosidase (AG) and xanthine oxidase (XO). The inhibitory activities of the three compounds against AChE, AG and XO was also evaluated. In addition, the enzymatic inhibitory activity of indole to the toxins from the venom of Bothrops asper was tested. Results showed that indole exhibited strong inhibitory activity to AG (IC50 = 18.65 ± 1.1 μM), to AChE, and XO (51.3% and 44.3% at 50 μg/mL, respectively). 1H-indole-3-carboxaldehyde displayed strong activity to XO (IC50 = 13.36 ± 0.39 μM). 4-hydroxybenzaldehyde showed moderate activity to XO (50.75% at 50 μg/mL) and weak activity to AChE (25.7% at 50 μg/mL). Furthermore, indole showed a significant in vitro inhibition to the coagulant effect induced by 1.0 μg of venom. The findings were supported by molecular docking. This is the first comprehensive report on the chemistry of V. neocaledonicus and the bioactivity of its metabolites.

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Antioxidant, alpha glucosidase and antibacterial evaluation of Syzygium mytifolium (Roxb.) Walp.

Plant Science Today ◽

10.14719/pst.2021.8.2.1113 ◽

2021 ◽

Vol 8 (2) ◽

Author(s):

Mahmoud Abdulrahman

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Ethanolic Extract ◽

Antioxidant Power ◽

Whole Plant ◽

Ferric Reducing Antioxidant Power ◽

High Antioxidant Activity ◽

Antioxidant Supplements ◽

Alpha Glucosidase

Medicinal plants have been the major source of combating and treating infectious diseases and health disorders throughout the ages. To explore new sources of inexpensive and safe antioxidants and antibiotics. The antioxidant and antibacterial potential of different extracts Syzygium mytifolium (Roxb.) Walp. were evaluated through 1,1-Diphenyl-2-Picryl Hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), ?-glucosidase inhibition, disk diffusion and agar well methods. The leaves, bark, root and whole plant of Syzygium mytifolium were extracted with various solvents viz ethanol, methanol and aqueous to give crude extracts, respectively. Among all, the ethanolic extract showed high antioxidant activity in terms of radical scavenging activity, reducing power and alpha-glucosidase inhibition at 1.84 µg/ml, 40.2 mmol/gm and 14.34 µg/ml, respectively. All the tested parts of the plant exhibited moderate to higher range of antibacterial activity against bacterial pathogens with the inhibition zones ranged between 2-27.8 mm. Syzygium mytifolium will serve as a very good source for the development of antioxidant supplements and antibiotics for addressing infectious pathogens and improvement of health status.

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LC-MS analysis, antioxidant and alpha-glucosidase inhibitory activities of Centaurea papposa extracts

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v14i4.41246 ◽

2019 ◽

Vol 14 (4) ◽

pp. 159-165 ◽

Cited By ~ 1

Author(s):

Cheribet Mawahib ◽

Zaabat Nabila ◽

Souilah Nabila ◽

Bensouici Chawki ◽

Akkal Salah

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Inhibitory Activity ◽

Video Clip ◽

Inhibitory Effect ◽

Frap Assay ◽

Antioxidant Power ◽

Ms Analysis ◽

Alpha Glucosidase

This work aimed to ascertain the phenolic compounds and assess the antioxidant capacity and alpha-glucosidase inhibitory activity of Centaurea papposa extracts. Phenolic compounds were appraised using LC-MS technique. Moreover, antioxidant activity was investigated using DPPH, ABTS, CUPRAC and FRAP assays. In vitro alpha-glucosidase inhibitory effect was carried out. LC-MS analysis revealed the presence of 21 compounds among which 13 were phenolic acids, 6 flavonoids, 1 phenolic aldehyde and 1 benzopyrone. The ethyl acetate extract exhibited the highest activity in ferric reducing antioxidant power (FRAP) assay (IC50: 22.9 ± 2.8 µg/mL). Nevertheless the n-butanol extract was the most active in cupric reducing antioxidant capacity assay (IC50: 3.1 ± 0.1 µg/mL). A significant alpha-glucosidase inhibitory activity was displayed by dichloromethane extract (IC50: 227.6 ± 4.4 µg/mL). Video Clip of Methodology: Cupric reduction antioxidant capacity: 1 min 47 sec: Full Screen Alternate

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Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees

Plants ◽

10.3390/plants10061191 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1191

Author(s):

Mahak Majeed ◽

Tanveer Bilal Pirzadah ◽

Manzoor Ahmad Mir ◽

Khalid Rehman Hakeem ◽

Hesham F. Alharby ◽

...

Keyword(s):

Antioxidant Activities ◽

Pearson Correlation ◽

Juglans Regia ◽

Radical Scavenging ◽

Reducing Power ◽

Viscum Album ◽

Total Phenol Content ◽

Leaf Extracts ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte Viscum album L. harvested from three tree species, namely, Populus ciliata L, Ulmus villosa L., and Juglans regia L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host Juglans regia L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in Viscum album L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.

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Formulation And Antioxidant Property Of Bitter Melon Seeds Oil Loaded Into SNEDDS Systems As A Nutraceutical

INDONESIAN JOURNAL OF PHARMACY ◽

10.22146/ijp.1334 ◽

2021 ◽

Author(s):

Lina Winarti ◽

Lusia Oktora Ruma Kumala Sari ◽

Evi Umayah Ulfa ◽

Dwi Ayu Samsuri

Keyword(s):

Antioxidant Activity ◽

Seed Oil ◽

Ph Value ◽

Reducing Power ◽

Antioxidant Property ◽

Bitter Melon ◽

Antioxidant Power ◽

Melon Seed ◽

Melon Seed Oil ◽

Melon Seeds

Bitter melon seeds oil is less soluble in the gastrointestinal tract and has low absorption. Therefore, a self-nanoemulsion dosage form needed to support its absorption and increase its stability. This study aimed to formulate bitter melon seeds oil into a self-nano emulsifying drug delivery system (SNEDDS) and evaluate its antioxidant activity using the Ferric Reducing Antioxidant Power (FRAP) method. The SNEDDS formulation uses bitter melon seed oil as the active ingredient and the oil phase, cremophor RH 40 as a surfactant, and glycerin as a co-surfactant. The results showed that the best SNEDDS formula obtains a ratio of oil: Smix (surfactant mixture) of 1:4. The best formula transmittance was 97.35 ± 0.04% with an emulsification time of 15.69 ± 0.06 seconds, a pH value of 6.87 ± 0.08, and a particle size of 31.8 ± 16.3 nm. Thermodynamic stability and robustness to dilution tests show the preparation is stable and resistant to various dilutions and pH. The antioxidant activity of bitter melon seed oil before and after being formulated into SNEDDS resulted in 62.73% and 50.31% reducing power. This result is not differences significantly. This study concluded that bitter melon seeds oil SNEDDS has good physical characteristics, stability, and no antioxidant activity changes.

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Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

The Scientific World JOURNAL ◽

10.1155/2018/7470384 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Kenza Ammor ◽

Dalila Bousta ◽

Sanae Jennan ◽

Bahia Bennani ◽

Abdellah Chaqroune ◽

...

Keyword(s):

Antibacterial Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Antibacterial Activities ◽

Diffusion Method ◽

Antioxidant Power ◽

Negative Effect ◽

Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

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ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF SELECTED PLANTS USED BY POPULATIONS FROM JURUENA VALLEY, LEGAL AMAZON, BRAZIL

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.17086 ◽

2017 ◽

Vol 9 (5) ◽

pp. 179

Author(s):

Larissa Irene Da Silva ◽

Arunachalam Karuppusamy ◽

Fabio Miyajima ◽

Ivana Maria Povoa Violante ◽

Isanete Geraldini Costa Bieski ◽

...

Keyword(s):

Antioxidant Activities ◽

Shigella Flexneri ◽

Antibacterial Activities ◽

Total Phenolic ◽

Moderate Activity ◽

Cedrela Odorata ◽

Frap Assay ◽

Antioxidant Power ◽

Mato Grosso

Objective: The purpose of this study was to evaluate selected Brazilian plants from Juruena valley region of Mato Grosso, for their in vitro antimicrobial and antioxidant activities.Methods: The powder obtained from different parts of the twenty-six (26) plants were macerated in hydroethanolic solution to obtain the extracts. The hydroethanolic extracts were tested for their in vitro antimicrobial activity by determining the MIC using broth microdilution. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, ferric reducing antioxidant power (FRAP) and nitric oxide (NO) methods were used for the determination of antioxidant activities. Correlation between classes of secondary metabolites and antioxidant activity was assessed.Results: Phanera glabra extract (HEPg) showed broad antibacterial spectrum, presenting the best activity against Klebsiella pneumoniae. Hevea microphylla extract (HEHm) presented a narrow spectrum of antibacterial activity with strong effect against Shigella flexneri. The only plant with broad spectrum antifungal activity was Bertholletia excelsa (HEBe), with moderate activity against strains of Aspergillus and Candida. The following extracts were prominent regarding their activities in the DPPH and FRAP assays-HEBe, Cariniana rubra (HECr) and in the FRAP assay alone, Cedrela odorata (HECo) and HEPg. None of the extracts was active in the NO assay. A significant association was observed between DPPH activity and the total phenolic contents.Conclusion: Our results justified the use of some of the investigated plants in the Brazilian ethnomedicine. The antibacterial activities of these plants are bacteriostatic in nature. These findings support that a number of investigated plants could be a valuable source of new antioxidant and antimicrobial compounds that can potentially deliver novel mechanisms of actions.However, further studies are required.

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ALPHA AMYLASE AND ALPHA GLUCOSIDASE INHIBITION ACTIVITY OF SELECTED EDIBLE SEAWEEDS FROM SOUTH COAST AREA OF INDIA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i6.17684 ◽

2017 ◽

Vol 9 (6) ◽

pp. 64 ◽

Cited By ~ 2

Author(s):

P. Reka ◽

Thahira Banu A. ◽

M. Seethalakshmi

Keyword(s):

Aqueous Extract ◽

Inhibitory Activity ◽

Alpha Amylase ◽

Postprandial Blood Glucose ◽

Inhibition Activity ◽

Gracilaria Edulis ◽

Edible Seaweeds ◽

Daily Diet ◽

Postprandial Blood Glucose Level ◽

Alpha Glucosidase

Objective: The present work was to investigate the alpha amylase and alpha-glucosidase inhibitory activity of the selected edible seaweeds.Methods: The seaweeds namely Acanthophora spicifera, Gracilaria corticata, Gracilaria edulis, Ulva lactuca and Ulva reticulata were selected for this study. Six and eight hours of ethanol and aqueous extract were used for the estimation of alpha amylase using DNS method and alpha-glucosidase inhibition activity.Results: The study reported that the solvent from ethanol and aqueous in eight hours of extraction showed a higher inhibitory activity than six hours of extraction. Maximum of 89.1±0.96 and 79.55±3.08 percent of alpha-amylase and alpha-glucosidase inhibition activity were detected in the eight hours of aqueous extract (0.5 ml) of Ulva reticulata and Gracilaria edulis respectively. All the selected edible seaweeds had significant differences (p<0.05) in alpha amylase and alpha glucosidase inhibition activity between the selected seaweeds with different extracts.Conclusion: It was concluded that all the selected edible seaweeds have the potential to act as a potent inhibitor of the carbohydrate hydrolyzing enzyme. Thus, it was clear from the study that seaweeds incorporated in small amounts in the dishes consumed in the daily diet can bring a control on postprandial blood glucose level.

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Nutritional Composition and .ALPHA.-Glucosidase Inhibitory Activity of Five Chinese Vinegars

Japan Agricultural Research Quarterly JARQ ◽

10.6090/jarq.45.445 ◽

2011 ◽

Vol 45 (4) ◽

pp. 445-456 ◽

Cited By ~ 5

Author(s):

JunFeng FAN ◽

YanYan ZHANG ◽

LinNa ZHOU ◽

ZaiGui LI ◽

BoLin ZHANG ◽

...

Keyword(s):

Inhibitory Activity ◽

Nutritional Composition ◽

Alpha Glucosidase

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Effect of packaging on phenols, flavonoids and antioxidant characteristics of mechanical cabinet dried wild pomegranate (Punica granatum L.) arils

Journal of Applied and Natural Science ◽

10.31018/jans.v13i1.2456 ◽

2021 ◽

Vol 13 (1) ◽

pp. 101-109

Author(s):

Abhimanyu Thakur ◽

NS Thakur ◽

Hamid ◽

Sunakshi Gautam

Keyword(s):

Antioxidant Activity ◽

Reducing Power ◽

Punica Granatum ◽

Total Phenols ◽

Antioxidant Power ◽

Chelating Activity ◽

Metal Chelating ◽

Temperature Conditions ◽

Metal Chelating Activity ◽

Punica Granatum L

Wild pomegranate (Punica granatum L.) fruit arils with high acid content are processed into dried arils (anardana) which is used as an ingredient in various medicinal formulations and culinary preparations. The various phenols and flavonoids are responsible for the antioxidant activity of anardana which signifies its functional benefits. The advanced packaging techniques like vacuum packaging, can retain higher antioxidant activity characteristics during storage. So, the present studies were carried out to compare the effect of packaging on total phenols, flavonoids, DPPH (2, 2-diphenyl-1-picrylhydrazyl) antioxidant activity, metal chelating activity, FRAP (ferric reducing antioxidant power) and reducing power of anardana prepared in mechanical cabinet drier. Anardana was packed in different packaging materials and stored under ambient and refrigerated temperature conditions. The overall effect of storage during 12 months revealed that there was a significant (p< 0.05) decrease in total phenols (180.95 to 161.76 mg GAE/100 g), flavonoids (40.60 to 32.66 mg QuE/100 g), DPPH antioxidant activity (61.23 to 56.13%), metal chelating activity (22.25 to 19.37%), FRAP (34.60 to 31.73 ?M Fe2+/100 g) and reducing power (0.610 to 0.521) which was observed less in anardana packed in ALPV (aluminium laminated pouches with vacuum) followed by ALP (aluminium laminated pouches) and gunny bags. Due to slower rates of degradation of various antioxidant compounds, the changes after 12 months of storage were lower under refrigerated conditions. The total phenols, flavonoids and DPPH antioxidant activity were reduced from 180.95 to 167.24 and 156.28 mg GAE/100 g, 40.60 to 34.95 and 30.37 mg QuE/100 g and 61.23 to 57.99 and 54.27 % in refrigerated and ambient temperature conditions, respectively.

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