scholarly journals Effect of packaging on phenols, flavonoids and antioxidant characteristics of mechanical cabinet dried wild pomegranate (Punica granatum L.) arils

2021 ◽  
Vol 13 (1) ◽  
pp. 101-109
Author(s):  
Abhimanyu Thakur ◽  
NS Thakur ◽  
Hamid ◽  
Sunakshi Gautam
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Punica Granatum ◽  
Total Phenols ◽  
Antioxidant Power ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Temperature Conditions ◽  
Metal Chelating Activity ◽  
Punica Granatum L

Wild pomegranate (Punica granatum L.) fruit arils with high acid content are processed into dried arils (anardana) which is used as an ingredient in various medicinal formulations and culinary preparations. The various phenols and flavonoids are responsible for the antioxidant activity of anardana which signifies its functional benefits. The advanced packaging techniques like vacuum packaging, can retain higher antioxidant activity characteristics during storage. So, the present studies were carried out to compare the effect of packaging on total phenols, flavonoids, DPPH (2, 2-diphenyl-1-picrylhydrazyl) antioxidant activity, metal chelating activity, FRAP (ferric reducing antioxidant power) and reducing power of anardana prepared in mechanical cabinet drier. Anardana was packed in different packaging materials and stored under ambient and refrigerated temperature conditions. The overall effect of storage during 12 months revealed that there was a significant (p< 0.05) decrease in total phenols (180.95 to 161.76 mg GAE/100 g), flavonoids (40.60 to 32.66 mg QuE/100 g), DPPH antioxidant activity (61.23 to 56.13%), metal chelating activity (22.25 to 19.37%), FRAP (34.60 to 31.73 ?M Fe2+/100 g) and reducing power (0.610 to 0.521) which was observed less in anardana packed in ALPV (aluminium laminated pouches with vacuum) followed by ALP (aluminium laminated pouches) and gunny bags. Due to slower rates of degradation of various antioxidant compounds, the changes after 12 months of storage were lower under refrigerated conditions. The total phenols, flavonoids and DPPH antioxidant activity were reduced  from 180.95 to 167.24 and 156.28 mg GAE/100 g, 40.60 to 34.95 and 30.37 mg QuE/100 g and 61.23 to 57.99 and 54.27 % in refrigerated and ambient temperature conditions, respectively.

scholarly journals Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Talal Sabraoui ◽  
Taleb Khider ◽  
Boubker Nasser ◽  
Rabiaa Eddoha ◽  
Abderrahman Moujahid ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Punica Granatum ◽  
Total Phenolic ◽  
Natural Food ◽  
Pomegranate Peel ◽  
Metal Chelating ◽  
Mediterranean Countries ◽  
Punica Granatum L

Pomegranate (Punica granatum L) is widely cultivated in the Mediterranean countries especially in Morocco. Pomegranate peel and seed contain considerable amounts of phenolic compounds with antioxidant activity. The aim of the present study was to phytochemically characterize the pomegranate peels and seeds obtained from three Moroccan provinces, using UHPLC-DAD. In addition, total phenolic content (TPC), total flavonoid contents (TFC), and metal chelating of pomegranate peel were also evaluated. The results showed that pomegranate peel possesses the highest phenolic (TPC: 224.39 mg GAE/g dw) and flavonoid (TFC: 62.64 mg rutin/g dw) contents. Punicalagin-β and punicalagin-α, are the abundant compounds found in peel: 216.36±9.94 mg/g, 154.94±5.21 mg/g, respectively. Pomegranate peels showed significantly (p<0.05) high antioxidant activity 1-diphenyl-2-picrylhydrazyl (DPPH) EC50: 42.71±0.04 μg/mL, 2.2′-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) EC50: 62.15±0.01 μg/mL), and chelating activity (FRAP 1.85±0.00 mg ascorbic acid equivalents/100 g, Fe2+: 2.52±0.01 μmol EDTA equivalents/g dw) compared to seeds. A positive correlation between antioxidant activity and total phenolic was found. According to achieved results, high antioxidant capacity of pomegranate extracts, especially peel, shed light to further use as natural food preservatives. Pomegranate peel could be used for the fortification of food with fiber by introducing it in dietary, as well as in health applications due to its higher antioxidant capacity.

scholarly journals Synthesis, Antimicrobial, and Antioxidant Activities of N-[(5′-Substituted-2′-phenyl-1H-indol-3′-yl)methylene]-5H-dibenzo[b,f]azepine-5-carbohydrazide Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Anand R. Saundane ◽  
Vijaykumar Tukaram Katkar ◽  
A. Verma Vaijinath
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Systematic Investigation ◽  
Klebsiella Pneumonia ◽  
Antioxidant Power ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Indole Compounds ◽  
Metal Chelating Activity

The main aim of the present study was to synthesize new leads with potential antimicrobial and antioxidant activities. As a part of systematic investigation of synthesis and biological activity, some new indole compounds3a–cand4a–cwere prepared and screened for their antimicrobial and antioxidant activities. The antimicrobial evaluation of newly synthesized compounds was carried out by cup-plate method. Antimicrobial activity results revealed that compound4ashowed promising activity against bacteriaStaphylococcus aureus,Klebsiella pneumonia, andPseudomonas aeruginosaand exhibited maximum inhibition againstAspergillus niger,Aspergillus oryzae,Aspergillus terreus, andAspergillus flavus. The antioxidant activity was performed by three methods, namely, radical scavenging activity (RSA), ferric ions (Fe+3) reducing antioxidant power (FRAP), and metal chelating activity by using Hatano’s, Oyaizu’s, and Dinis' methods, respectively. Compound4ashowed promising RSA, FRAP, and metal chelating activity.

scholarly journals Studies on antioxidant activity of red, white, and black pomegranate (Punica granatum L.) peel extract using DPPH radical scavenging method

2021 ◽  
Vol 5 (2) ◽  
pp. 51-55
Author(s):  
Uswatun Chasanah
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Dpph Radical ◽  
Pomegranate Peel ◽  
Antioxidant Power ◽  
Pomegranate Peel Extract ◽  
Dpph Radical Scavenging ◽  
Punica Granatum L ◽  
Peel Extract

Pomegranate (Punica granatum L.) has high antioxidant activity. In Indonesia, there are red pomegranate, white pomegranate, and black pomegranate. The purpose of this study was to determine the antioxidant activity of red pomegranate peel extract, white pomegranate peel extract, and black pomegranate peel extract. The extracts prepared by ultrasonic maceration in 96% ethanol, then evaporated until thick extract was obtained and its antioxidant activity was determined using the DPPH radical scavenging method. This study showed that all pomegranate peel extract varieties have potent antioxidant activity and the black pomegranate peel extract has the highest antioxidant power.

scholarly journals Structural Insight and in vitro Free Radical Scavenging Capacity of Arabinogalactan Polysaccharides from the Peels of Punica granatum

2021 ◽  
Vol 33 (4) ◽  
pp. 873-880
Author(s):  
Dipan Adhikari ◽  
Tuhin Ghosh
Keyword(s):  
Antioxidant Activity ◽  
Molecular Mass ◽  
Radical Scavenging ◽  
Water Extract ◽  
Punica Granatum ◽  
Antioxidant Power ◽  
Structural Insight ◽  
Ethanol Ethanol ◽  
Punica Granatum L

The juice extracted from Punica granatum L. fruit has been used for ages as an important functional food that can endow with healthcare benefits besides fundamental nutritional food that we intake. Although demonstration of antioxidant activity has been shown here, the target molecule responsible for this phenomenon remains unidentified. In this present work, we report a structural insight and antioxidant activity of its polysaccharide (PF-1), purified from water extract (WE) by precipitation with ethanol, ethanol soluble fraction (PF-2) and acetone extracted fraction (PF-3). The purified polysaccharide (PF-1) contains mainly arabinose, galactose together with lesser amount of rhamnose and glucose residues, and the molecular mass was determined 180 kDa. This fraction consists of T-(1,5)- and (1,3,5)-linked Araf; T-(1,6)- and (1,3,6)-linked Galp alongside (1,2,4)-linked Rhap residues. The glucan is found to be β-(1→3)-linked glucopyranosyl residues with a molecular mass of 9 kDa. The in vitro antioxidant activity of the active fractions was determined by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), hydroxyl radical scavenging and total antioxidant activity (TAA) assays. The arabinogalactan showed the highest potential among the polysaccharides. This work involves an economically viable simple extraction method and reports promising antioxidant capacity. The studies suggest that this polysaccharide from Punica granatum L. fruit could be a natural antioxidant.

Functional and Antioxidant Properties of Angelwing Clam (Phplas orientalis) Hydrolysate Produced using Alcalase

2014 ◽  
Vol 11 (1) ◽  
pp. 29
Author(s):  
Nonnah Ismail ◽  
Juliana Mahmod ◽  
Awatif Khairul Fatihin Mustafa Kamal
Keyword(s):  
Antioxidant Properties ◽  
Enzyme Concentration ◽  
Degree Of Hydrolysis ◽  
Emulsifying Properties ◽  
Hydrolysis Time ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Metal Chelating Activity ◽  
Intermediate Concentration ◽  
As Solubility

In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of0.5 and 3%using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties such as solubility, emulsifying properties and water and oil holding capacity. Degree of hydrolysis (DH), yield, functional and antioxidant properties were influenced by the hydrolysis time and E/S ratio. Higher enzyme concentration (E/S 3%) and longer hydrolysis time increased the DH. Yield was higher at E/S 3% but reduced with hydrolysis time. Longer hydrolysis time produced more soluble hydrolysate and higher metal chelating activity but lower in emulsifying properties and DPPH activity. Higher enzyme concentration resulted in increase only in solubility and metal chelating activity. This study revealed that enzymatic hydrolysis using alcalase should be performed at shorter hydrolysis time using intermediate concentration of enzyme (E/S between 0.5 to 3%) in order to produce angelwing clam hydrolysate with collectively good functional and antioxidant properties. 

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT ACTIVITY AND ACUTE ORAL TOXICITY OF POMEGRANATE (PUNICA GRANATUM L.) FRUIT PEEL EXTRACT

2019 ◽  
pp. 7-14
Author(s):  
Hai Trieu Ly ◽  
Tuan Anh Vo ◽  
Viet Hong Phong Nguyen ◽  
Thi My Sa Pham ◽  
Bich Thao Lam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Punica Granatum ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Fruit Peel ◽  
Total Polyphenol ◽  
Punica Granatum L

Background: The natural antioxidants have an important role in the prevention of many diseases. The aim of study is to investigate phytochemical components, antioxidant activity and acute oral toxicity of Pomegranate (Punica granatum L.) fruit peel (PFP) extract. Materials and methods: Phytochemicals of PFP were determined by qualitative chemical tests, thin layer chromatography, total polyphenol and flavonoid contents. The PFP extract was evaluated for antioxidant activity by DPPH assay and MDA assay. In vivo acute oral toxicity test was conducted using Karber-Behrens method to determine LD50. Results: Results illustrated that PFP mainly contains flavonoids, alkaloids, tannins, triterpenes, saponins, and coumarins. PFP extract exhibited the total polyphenol and flavonoid contents with 189.97 mg gallic acid equivalent/g dry weight and 9.42 mg quercetin equivalent/g dry weight, respectively. The DPPH free radical scavenging and anti-lipid peroxidation activities of PFP extract were expressed with IC50 value of 4.80 μg/mL and 0.38 μg/ mL, sequentially. Simultaneously, the Dmax (the maximum dose administered to mice that no toxicity was observed) of PFP extract was determined to be 21.28 g/kg, equivalent to 35.64 g dried herb. Conclusion: The PFP extract is relatively safe and revealed high antioxidant activity. Key words: Punica granatum L.; polyphenols; flavonoids; gallic acid; quercetin; antioxidant activity; acute oral toxicity

Formulation And Antioxidant Property Of Bitter Melon Seeds Oil Loaded Into SNEDDS Systems As A Nutraceutical

2021 ◽  
Author(s):  
Lina Winarti ◽  
Lusia Oktora Ruma Kumala Sari ◽  
Evi Umayah Ulfa ◽  
Dwi Ayu Samsuri
Keyword(s):  
Antioxidant Activity ◽  
Seed Oil ◽  
Ph Value ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bitter Melon ◽  
Antioxidant Power ◽  
Melon Seed ◽  
Melon Seed Oil ◽  
Melon Seeds

Bitter melon seeds oil is less soluble in the gastrointestinal tract and has low absorption. Therefore, a self-nanoemulsion dosage form needed to support its absorption and increase its stability. This study aimed to formulate bitter melon seeds oil into a self-nano emulsifying drug delivery system (SNEDDS) and evaluate its antioxidant activity using the Ferric Reducing Antioxidant Power (FRAP) method. The SNEDDS formulation uses bitter melon seed oil as the active ingredient and the oil phase, cremophor RH 40 as a surfactant, and glycerin as a co-surfactant. The results showed that the best SNEDDS formula obtains a ratio of oil: Smix (surfactant mixture) of 1:4. The best formula transmittance was 97.35 ± 0.04% with an emulsification time of 15.69 ± 0.06 seconds, a pH value of 6.87 ± 0.08, and a particle size of 31.8 ± 16.3 nm. Thermodynamic stability and robustness to dilution tests show the preparation is stable and resistant to various dilutions and pH. The antioxidant activity of bitter melon seed oil before and after being formulated into SNEDDS resulted in 62.73% and 50.31% reducing power. This result is not differences significantly. This study concluded that bitter melon seeds oil SNEDDS has good physical characteristics, stability, and no antioxidant activity changes.

scholarly journals Comparison of five solvents in the extraction of phenolic anti-oxidants from pomegranate (Punica granatum L.) peel

2019 ◽  
Vol 3 (5) ◽  
pp. 140-147
Author(s):  
Abderrezak Kennas ◽  
Hayat Amellal-Chibane
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Punica Granatum ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
Punica Granatum L ◽  
Extracting Solvent ◽  
High Inhibition

Background: Pomegranate peels are rich in bioactive compounds and could be an alternative natural source such as antioxidants. Aims: The aim of the present study was to assess the abilities of five solvents to extract phenolic antioxidants from pomegranate peels. Methods and Material: Pomegranate peels powder was subjected to extraction and the extraction yield was compared. The total phenolic, flavonoid, condensed and hydrolysable tannins contents were analyzed. The antioxidant activity of the extracts was evaluated by two methods (DPPH* and ABTS•+ scavenging capacities) and results were then compared. Results: Results showed that the mixture methanol/water (50:50) allowed higher extraction yield (37.33±5.3%) than the others solvents (P<0.05). Further, the total phenolic, flavonoid and condensed tannins contents were the highest in mixture water/methanol (50:50) extract. Phenolic antioxidants showed a distinct reducing capacity and a high DPPH* inhibition values were recorded for all extracts with no significant differences (P>0.05) between ethanol and mixture water/methanol (50:50) extracts. All extracts exhibited high inhibition against ABTS•+ but with a considerable variation. Phenolic content and antioxidant activities were well positively correlated with each other. Conclusions: Our findings revealed that the choice of the extracting solvent affects considerably the extraction of phenolic antioxidants from pomegranate peels. Keywords: Pomegranate peels, solvent extraction, antioxidant activity, phenolic antioxidants.

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