ISOLATION AND CHARACTERIZATION OF AMMONIA EXCRETING CYANOBACTERIUM CYLINDROSPERMUM SP. NDOP002

Cylindrospermum sp. NDOP002 was isolated from agricultural fields of Azamgarh, U.P., India. It was characterized by morphological methods. Total chlorophyll a content was 18.5 µg/ mg dry weight. Organism achieved a stationary phase of growth after 15 days of cultivation. Ammonia excretion was monitored from 6 hrs. of culture to 20 days. The ammonia content of culture increased up to 7 days (Maximum amount of 3.23 µg/ ml) and then decreased. Approximately a constant amount of ammonia was maintained from 9th and onwards days of growth. Maximum glutamine synthetase (GS) activity of 8.33 mM ?glutamyl hydroxamate min-1 mg chl-1 was observed on the 5th day of culture and then decreased. Approximately constant GS activity was observed after 9th and onward days of growth. Algalisation experiment showed an increase in growth parameter of rice plant in algalised set. Length of roots was 6.4-6.9 cm in non algalised and 7.2-7.6 cm in algalised rice plants whereas the length of shoots was 5.7-6.1 cm in non- algalised and 9.5-10.1 cm in algalised rice plants. The amount of ammonia in algalised set was 2.1 µg/ ml on the 10th day of the experiment. Experimental findings clearly suggest Cylindrospermum sp. NDOP002 as suitable inocula for algalisation of rice fields of Azamgarh district, U.P., India.

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ISOLATION AND PHYSICOCHEMICAL CHARACTERIZATION OF STARCH FROM TARO BOLOSO-I TUBERS

INDIAN DRUGS ◽

10.53879/id.55.07.11391 ◽

2018 ◽

Vol 55 (07) ◽

pp. 20-27

Author(s):

Tamrat Balcha ◽

◽

Nisha Mary Joseph ◽

Anteneh Belete

Keyword(s):

Agricultural Research ◽

Potato Starch ◽

Physicochemical Characterization ◽

Dry Weight ◽

Weight Basis ◽

Rice Starch ◽

Small Particles ◽

New Variety ◽

Isolation And Characterization

Taro Boloso-I is a new variety of Colocasia esculenta (L. Schott) officially released from Areka Agricultural Research Center, Areka, Ethiopia. Its cultivation out yields a previously reported variety (Godare) by 67%. The study focuses on isolation and characterization of starch from this plant. Isolation of starch was done using saline and sodium hydroxide and characterized for its physicochemical constituents. Its yield on dry weight basis was 83.5 ± 1.6%. The native Taro Boloso-I starch (NTBIS) showed lower amylose to amylopectin ratio (20.7 ± 1.8% to 77.3 ± 2.1%, w/w) and significantly higher onset (68.40˚C), peak (75.46 ˚C) and endset (84.40 ˚C) temperatures of gelatinization than potato starch which has gelatinization range of 62.50–67.53–79.40 ˚C. The shapes of the granules were polyhedral/angular with an A-type polymorphism with a poor flow.The starch is comprised of small particles (mean diameter of 2.45 ± 0.11 μm).Taro Boloso-I starch possesses similarity with cereal starches like the rice starch though it is a tuber starch and moreover, on comparison with the previously reported varieties of Taro, there is difference in its properties.

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Isolation and Characterization of Pectin from Pumpkin (Cucurbita maxima) Waste and Its Food Application

Asian Food Science Journal ◽

10.9734/afsj/2019/v13i230104 ◽

2019 ◽

pp. 1-9

Author(s):

Jayani Sandarani Maddakandage Dona

Keyword(s):

Acid Hydrolysis ◽

Activity Index ◽

Dry Weight ◽

Reaction Conditions ◽

Isolation And Characterization ◽

Significant Difference ◽

Food Applications ◽

Methoxy Content ◽

Water Holding

Pectin was extracted using the method of acid hydrolysis followed by ethanol precipitation at various reaction conditions. Effect of blanching prior to acid hydrolysis, acid types (hydrochloric and citric) and extraction time and temperature combinations were tested to maximize the yield of pectin and they were characterized by assessing physiochemical and functional properties. The feasibility of utilizing extracted pectin in food applications was evaluated. The yield of pectin ranged from 7.30 ± 0.07- 11.04 ±0.01% on a dry weight basis. The results suggested that the highest pectin content is in the fresh pumpkin peel extracted using 0.1 N hydrochloric acid at 80±5℃, 1.25 pH for 1 hour. Water holding capacity, oil holding capacity and emulsifying activity index of pumpkin pectin were 2.5 g/g of pectin (SD 0.46), 1.76 g/g of pectin (SD 0.10) and 0.29 (SD 0.01) respectively. In addition, the degree of esterification, equivalent weight, methoxy content and galacturonic acid content of extracted pectin were 67.64% (SD 0.89%), 978.35 (SD 69.88), 6.55% (SD 0.37%) and 66.46% (SD 1.19%) respectively. Sensory analysis revealed that there is no significant difference in spreadability, surface texture and chewiness of two types of jam prepared using pumpkin and commercial pectin. In conclusion, pumpkin pectin can be categorized as high methoxy pectin.

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Isolation and characterization of a factor from Bipolaris zeicola race 3 that induces susceptibility in rice plants.

Japanese Journal of Phytopathology ◽

10.3186/jjphytopath.56.605 ◽

1990 ◽

Vol 56 (5) ◽

pp. 605-612 ◽

Cited By ~ 1

Author(s):

Jin-zhong XIAO ◽

Shin-ichi NAKATSUKA ◽

Mitsuya TSUDA ◽

Noriyuki DOKE ◽

Syoyo NISHIMURA

Keyword(s):

Rice Plants ◽

Isolation And Characterization ◽

Bipolaris Zeicola

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Isolation and Characterization of Pepsin-Solubilized Collagen from Skin of Argentine Shortfin Squid (Illex argentinus)

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.19 ◽

2011 ◽

Vol 236-238 ◽

pp. 19-26 ◽

Cited By ~ 1

Author(s):

Jun Jie Zhang ◽

Rui Duan ◽

Yi Feng Chen ◽

Xiao Jing Xu

Keyword(s):

Dry Weight ◽

Biochemical Properties ◽

Commercial Fish ◽

Isolation And Characterization ◽

Collagen Denaturation ◽

Sds Page ◽

Skin Collagen ◽

Illex Argentinus ◽

Peptide Maps

Argentine shortfin squid (Illex argentinus) is one of the most important commercial fish in the world. Pepsin-solubilized collagen (PSC) was isolated from squid skin and biochemical properties of PSC were studied. The yields of skin PSC was about 18.5 % (on the dry weight basis). SDS-PAGE patterns showed that the collagen consisted of at least two different polypeptides (α1 and α2 chains). The peptide maps of PSC digested by pepsin were distinct from those of carp skin collagen. Denaturation temperatures, measured by melting point using circular dichroism, was 26.5 °C.The results suggest that squid skin collagen has potential as a possible underutilized resource used in various fields.

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Isolation and Characterization of Azospirillum Strains from Soil and Rice Plants in North Vietnam

Azospirillum VI and Related Microorganisms ◽

10.1007/978-3-642-79906-8_64 ◽

1995 ◽

pp. 559-566 ◽

Cited By ~ 1

Author(s):

Nguyen ngoc Dung ◽

Maria Kraut ◽

Walter Klingmüller

Keyword(s):

Rice Plants ◽

Isolation And Characterization ◽

North Vietnam

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Isolation and characterization of cellulose nanocrystals from jackfruit peel

Scientific Reports ◽

10.1038/s41598-019-53412-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 14

Author(s):

C. Trilokesh ◽

Kiran Babu Uppuluri

Keyword(s):

Cellulose Nanocrystals ◽

Dry Weight ◽

Xrd Analysis ◽

Sodium Chlorite ◽

Nmr Spectrum ◽

Isolation And Characterization ◽

Endothermal Peak ◽

Edible Parts ◽

C Nmr

Abstract In the present work, sustainable nanomaterials, cellulose, and spherical cellulose nanocrystals (SCNCs) were isolated from the non-edible parts of jackfruit (Artocarpus heterophyllus). Of the three different methods tested, sodium chlorite treatment produced the highest yield of cellulose, 20.08 ± 0.05% w/w (dry weight). Peaks observed in CP/MAS 13C NMR spectrum and FTIR frequencies revealed the presence of α-cellulose and absence of other biomass fractions like hemicellulose and lignin. XRD analysis showed a high crystallinity of 83.42%. An appearance of a sharp endothermal peak at 323 °C in DSC and decomposition patterns between 310–420 °C of TGA confirms the presence of cellulose. Further, Sulphuric acid hydrolysis was employed to produce SCNCs and examined by TEM for the morphology and by HPLC for the presence of glucose.

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Isolation and characterization of fungi from deteriorated old manuscripts from Banyumas, collection of Library of Universitas Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/948/1/012031 ◽

2021 ◽

Vol 948 (1) ◽

pp. 012031

Author(s):

W Lintang ◽

T Susetyo-Salim ◽

A Oetari ◽

W Sjamsuridzal

Keyword(s):

Electron Microscopy ◽

Fungal Spores ◽

Carbon Sources ◽

Morphological Characteristics ◽

Fungal Growth ◽

Dry Weight ◽

Isolation And Characterization ◽

Fungal Isolates ◽

Scanning Electron

Abstract Fungi are the main cause of old manuscript deterioration since manuscripts provide carbon source and nutrient for fungal growth. Isolation of fungi from deteriorated old manuscripts from Banyumas was carried out and their morphology, xerophilic, and cellulolytic nature were investigated. Two deteriorated old dluwang manuscripts showed fungal spores, brown spots, and discoloured paper. Based on morphological characteristics, 31 fungal isolates belonged to five genera (Aspergillus Micheli, Cladosporium Link, Curvularia Boedijn, Penicillium Link, Ulocladium Preuss). These genera have been reported from deteriorated old manuscripts from several historical places in Indonesia. Xerophilic character was shown by 90% (28 isolates) as determined by growth in DG18 medium, which indicated the ability to grow in dry substrates such as old manuscripts. Cellulolytic character was shown by 93.5% (29 isolates) as determined by growth in dluwang paper and merang paper, which indicated that the papers were used as carbon sources and substrates. After 30 days-incubation, the dry weight loss of merang paper was 0.28-51.2%. Result from Scanning Electron Microscopy showed that the deterioration of merang paper were caused by the isolates as shown by the presence of fungal structures. These results showed that the fungal isolates were able to deteriorate old manuscripts from Banyumas, Indonesia.

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Characterization of frozen hydrated biological specimens by low-loss EELS

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100132492 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1572-1573

Author(s):

Songquan Sun ◽

Richard D. Leapman

Keyword(s):

Water Content ◽

Direct Method ◽

Internal Standard ◽

Dry Weight ◽

Dark Field ◽

Protein Solutions ◽

Subcellular Compartment ◽

Differential Shrinkage ◽

Electron Energy Loss

Analyses of ultrathin cryosections are generally performed after freeze-drying because the presence of water renders the specimens highly susceptible to radiation damage. The water content of a subcellular compartment is an important quantity that must be known, for example, to convert the dry weight concentrations of ions to the physiologically more relevant molar concentrations. Water content can be determined indirectly from dark-field mass measurements provided that there is no differential shrinkage between compartments and that there exists a suitable internal standard. The potential advantage of a more direct method for measuring water has led us to explore the use of electron energy loss spectroscopy (EELS) for characterizing biological specimens in their frozen hydrated state.We have obtained preliminary EELS measurements from pure amorphous ice and from cryosectioned frozen protein solutions. The specimens were cryotransfered into a VG-HB501 field-emission STEM equipped with a 666 Gatan parallel-detection spectrometer and analyzed at approximately −160 C.

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