USE OF FEMOFLOR-16 TEST TO ASSESS GENITAL BIOCENOSIS IN WOMEN WITH INFLAMMATORY AND PROLIFERATIVE DISEASES OF CERVIX

2016 ◽  
Vol 1 (61) ◽  
pp. 90-95
Author(s):  
Иевлева ◽  
Nadezhda Ievleva ◽  
Пермина ◽  
Natalya Permina ◽  
Ивахнишина ◽  
...  
Keyword(s):  
Genital Tract ◽  
Inflammatory Diseases ◽  
Female Genital Tract ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Healthy Women ◽  
Opportunistic Bacteria ◽  
Obligate Anaerobic ◽  
Atopobium Vaginae

Qualitative and quantitative assessment of microbes making the microbiocenosis of genital tract in women with inflammatory and proliferative diseases of cervix using Femoflor-16 test was the aim of the research. Scrapings of cervix and vaginal fornix in 100 women of reproductive age with cervicitis, vaginitis and in 31 women with cervical pseudoerosion (ectropion) were studied. The control group consisted of 35 relatively healthy women preparing for pregnancy. Cervical and vaginal dysbiosis was found in women with inflammatory diseases of cervix in 37.0% of cases, in women with pseudoerosion in 32.2% of cases. These are 3.3 (р<0.005) and 2.8 (р<0.02) times as much as in the group of healthy women preparing for pregnancy (11.1%). Dysbiosis structure was represented primarily by obligate anaerobic agents such as Gardnerella vaginalis, Atopobium vaginae, Eubacterium spp. in association with other opportunistic bacteria that are clinically most significant microorganisms colonizing female genital tract. Mycoplasma and yeast-like fungi of Candida species were found primarily with anaerobes. Aerobic and mixed dysbiosis were only found in 7% of cases in women with inflammatory diseases. Femoflor-16 test is a readily available, fast, efficient, up-to-date method enabling one to begin with early adequate antibacterial therapy and monitor it.

scholarly journals Peculiarities of microbiological monitoring in the pathological status of the vegina and cervix in women of reproductive age

2018 ◽  
Vol 22 (1) ◽  
pp. 163-167
Author(s):  
N.P. Dzis
Keyword(s):  
Genital Tract ◽  
Inflammatory Diseases ◽  
Hpv Infection ◽  
Reproductive Age ◽  
Vaginal Candidiasis ◽  
Gardnerella Vaginalis ◽  
Women Of Reproductive Age ◽  
E Coli ◽  
Gynecological Examination ◽  
Cervical Cyst

Vulvovaginal and cervical infections are a risk factor of developing purulent inflammatory diseases of the pelvic organs, miscarriage, antenatal and intranatal infections, with frequent pathogens Ureaplasma, Mycoplasma, Gardnerella vaginalis, Bacterioides. Particular importance is given to the mix-infection as a reservoir of a large number of different potentially harmful microorganisms. The purpose of our study was to study the specific composition of microorganisms in women of reproductive age with pathological secretions from the genital tract and to determine the incidence of genitalurea / mycoplasmosis. 54 patients of reproductive age with complaint on itch, heartburn, excessive secretions from the non-slippery genital tract and / or with an unpleasant smell, which were repeated 2 or more times during the year after the treatment, were examined. During gynecological examination, the following diseases were detected in patients: erosion of the cervix in 8 (14.8%) patients, cervicitis in 6 (11.1%), cervical cyst in 2 (3.7%) women, leukoplakia in 3 (5,55%) of the surveyed. According to the results of bacterioscopy, bacterial vaginosis was detected in 4 (7.4%) patients, unspecified bacterial colpitis — 28 (51.8%), vaginal candidiasis — in 17 (31.5%). In cytological screening, CIN I was diagnosed in 9 (16.6%) subjects, CIN II in 4 (7.4%). Analyzing the results of the study conducted by the polymerase chain reaction of HPV-infection was found in 8 (14,8%), ureaplasma — in 14 (25,9%), mycoplasma — in 6 (11,1%) patients. Staphylococcus aureus 105 — 4 (7.4%), Staphylococcus epidermidis 103 — 1 (1.8%), E. coli 105 — 7 (12.9%), Enterococcus, were detected during bacteriological examination of vaginal and cervical extracts. Fecalis 106 — 9 (16.6%), Candida albicans 105 — 6 (11.1%), Clebsiela 106 — 3 (5.5%), Gardnerella vaginalis 107 —11 (20.3%), Streptococcus 106 — 5 (9.2%). Thus, diagnosed high percentage (22.2%) ureaplasmas combination with other types of conditional — pathogenic microorganisms and viruses and cervical pathology (13%), namely the combination of ureaplasma pathogenic microflora was observed in 8 (14.8%) cases of HPV — 6 (11.1%), with mycoplasma, and pathogenic microflora — 5 (9.3%) patients. In general, 35.2% of cases. Assessing the infection of reproductive age women ureaplasma and mycoplasma less with recurrent abnormal discharge from the genital tract after treatment leads to the search and introduction of modern methods of their diagnosis and treatment.

Features of the ovarian reserve of women of reproductive age suffering from autoimmune diseases

GYNECOLOGY ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. 27-30
Author(s):  
Elena N. Andreeva ◽  
Olga R. Grigoryan ◽  
Yulia S. Absatarova ◽  
Irina S. Yarovaya ◽  
Robert K. Mikheev
Keyword(s):  
Type 1 Diabetes ◽  
Thyroid Gland ◽  
Autoimmune Diseases ◽  
Ovarian Reserve ◽  
Reproductive Potential ◽  
Reproductive Age ◽  
Control Group ◽  
Antithyroid Antibodies ◽  
Healthy Women

The reproductive potential of a woman depends on indicators of the ovarian reserve, such as the anti-Muller hormone (AMH) and the number of antral follicles (NAF). Autoimmune diseases have a significant effect on fertility and contribute to the development of premature ovarian failure. Aim.To evaluate the parameters of the ovarian reserve in patients with type 1 diabetes mellitus, carriers of antibodies to the thyroid gland in a state of euthyroidism and compare them with similar parameters in healthy women. Materials and methods.In the first block of the study, the level of AMH, follicle-stimulating hormone, luteinizing hormone, NAF was studied among 224 women with diabetes and 230 healthy women in the control group. In block II, the level of the above hormonal indices was studied in 35 carriers of antithyroid antibodies in the state of euthyroidism and 35 healthy women. Results.In patients with type 1 diabetes, the level of AMH, NAF was statistically significantly lower when compared with the control group. Among carriers of antithyroid antibodies and healthy women, no difference in AMH and NAF was found. Conclusion.The autoimmune processes accompanying diabetes are more influenced by the ovarian reserve indices than autoimmune aggression to the tissues of the thyroid gland.

scholarly journals Vaginal, Cervical and Uterine pH in Women with Normal and Abnormal Vaginal Microbiota

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 90
Author(s):  
Malene Risager Lykke ◽  
Naja Becher ◽  
Thor Haahr ◽  
Ebbe Boedtkjer ◽  
Jørgen Skov Jensen ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Genital Tract ◽  
Quantitative Polymerase Chain Reaction ◽  
Female Genital Tract ◽  
Uterine Cavity ◽  
Vaginal Microbiota ◽  
Reproductive Age ◽  
Ph Gradient ◽  
Increased Risk ◽  
Female Genital

Introduction: Healthy women of reproductive age have a vaginal pH around 4.5, whereas little is known about pH in the upper genital tract. A shift in the vaginal microbiota may result in an elevated pH in the upper genital tract. This might contribute to decreased fertility and increased risk of preterm birth. Therefore, we aimed to measure pH in different compartments of the female genital tract in both nonpregnant and pregnant women, stratifying into a normal and abnormal vaginal microbiota. Material and methods: In this descriptive study, we included 6 nonpregnant, 12 early-pregnant, and 8 term-pregnant women. A pH gradient was recorded with a flexible pH probe. An abnormal vaginal microbiota was diagnosed by a quantitative polymerase chain reaction technique for Atopobium vaginae; Sneathia sanguinegens; Leptotrichia amnionii; bacterial vaginosis-associated bacterium 1, 2, 3, and TM7; and Prevotella spp. among others. Results: In all participants we found the pH gradient in the lower reproductive canal to be most acidic in the lower vagina and most alkaline in the upper uterine cavity. Women with an abnormal vaginal microbiota had an increased pH in the lower vagina compared to the other groups. Conclusions: There is a pronounced pH gradient within the female genital tract. This gradient is not disrupted in women with an abnormal vaginal microbiota.

scholarly journals Acute abdomen in a patient with Mayer-Rokitansky-Kuster-Hauser syndrome

2008 ◽  
Vol 65 (9) ◽  
pp. 706-709 ◽  
Author(s):  
Aleksandra Petric ◽  
Milan Stefanovic ◽  
Predrag Vukomanovic ◽  
Radomir Zivadinovic ◽  
Aleksandra Tubic ◽  
...  
Keyword(s):  
Acute Abdomen ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Careful Examination ◽  
Normal Female ◽  
Vascular Leiomyoma ◽  
Primary Amenorrhoea ◽  
Healthy Women ◽  
Dead End ◽  
Female Genital

Background. Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome is a malformation of female genital tract (incidence 1 in 4000 female newborn children). It appears as a result of a disorder in the development of Millerian cannals. Etiology is unknown. Syndrome MRKH is the most frequent cause of primary amenorrhoea (90%). Patients with MRKH have a normal female phenotype, with normal pubic hairness and thelarche, and female karyotype (46XX) followed by primary amenorrhoea. Hormonal status corresponds to healthy women, where the appearance of ovarian tumors and tumors on rudiment parts of uterus is possible. Case report. We presented a case of acute abdomen in a patient with previously not diagnosed MRKH. The diagnosis was done during the operation. Small pelvis and an abdominal part were filled with torquated tumor lump, where ovaries, oviducts, uterus or something resembling rudiment of uterus were not recognized through careful examination. Furthemore, the patient had a short, dead-end vagina. Tumorectomy was done and hystopathological finding showed the presence of vascular leiomyoma. Conclusion. The diagnosis of complex syndromes, such as MRKH, can, despite modern diagnostics, be absent for non-medical and psycho-social reasons. We can expect ovarian and uterine pathology on hypoplastic structures in these patients, as well as in healthy women. Vascular leiomyoma in the patients with MRKH was not found in the available literature.

scholarly journals The skin microbiota in pregnant women suffering from atopic dermatitis

2016 ◽  
Vol 97 (2) ◽  
pp. 222-229
Author(s):  
A Yu Lonshakova-Medvedeva ◽  
K N Monakhov ◽  
A N Suvorov ◽  
O V Lavrova
Keyword(s):  
Staphylococcus Aureus ◽  
Atopic Dermatitis ◽  
Pregnant Women ◽  
Bacterial Load ◽  
Life Quality ◽  
Reproductive Age ◽  
Control Group ◽  
Skin Microbiota ◽  
Healthy Women ◽  
Basic Care

Aim. To study the skin microbiota of pregnant women suffering from atopic dermatitis.Methods. 53 women of reproductive age suffering from atopic dermatitis (28 pregnant and 25 non-pregnant) were examined. The control group included dermatologically healthy women (25 pregnant and 25 non-pregnant). Prior to treatment initiation and on 15-day of study pathological process spread, the SCORAD index (scoring of atopic dermatitis - atopic dermatitis severity assessment), dermatology life quality index determination were conducted. In addition, microbiological study of material taken from the forehead, elbow bend skin and visually unaltered forearm skin was performed.Results. In women (pregnant and non-pregnant), suffering from atopic dermatitis skin total bacterial load is increased. In all groups, the skin microbiota is presented mainly by staphylococci: in dermatologically healthy people - coagulase-negative, in atopic dermatitis - Staphylococcusя aureus. In atopic dermatitis Staphylococcus aureus is isolated from both lesions and visually unaltered skin. In pregnant women with atopic dermatitis skin bacterial load was higher, Staphylococcus aureus was found more commonly. The skin microbiota in dermatologically healthy women was more diverse in respect of species comparing with patients with atopic dermatitis. Basic care remedies use leads to clinical improvement and a decrease in the skin total bacterial load and Staphylococcus aureus load. Daily use of emollients has no effect on saprophytic microorganisms.Conclusion. In pregnant patients with atopic dermatitis higher skin total bacterial load and higher rate of skin colonization by Staphylococcus aureus are observed.

scholarly journals Mullerian adenosarcoma of the cervix: case report

2016 ◽  
Vol 6 (1) ◽  
pp. 335
Author(s):  
Akinfenwa T. Atanda ◽  
Osondu C. Agu ◽  
Abubakar K. Modu K. Modu
Keyword(s):  
Genital Tract ◽  
Clinical Presentation ◽  
Female Genital Tract ◽  
Reproductive Age ◽  
Age Group ◽  
Rare Tumours ◽  
Histopathological Evaluation ◽  
Optimum Management ◽  
Reproductive Age Group ◽  
Female Genital

Mullerian adenosarcomas are rare tumours that may be found in any part of the female genital tract including cervix. They are more common in the reproductive age group and rare among blacks. When they present in the cervix they may be clinically misdiagnosed as an endocervical polyp and histologically as an adenofibroma. Clinical presentation is mostly as irregular vagina bleeding and as a mass in the vagina. Meticulous histopathological evaluation is crucial for its optimum management.

LOWER GENITAL TRACT INFECTIONS OF REPRODUCTIVE AGE WOMEN AT HUE UNIVERSITY HOSPITAL

2018 ◽  
Vol 8 (5) ◽  
pp. 102-107
Author(s):  
Nhut Nguyen Tien ◽  
Huong Le Lam
Keyword(s):  
Genital Tract ◽  
Parasitic Infection ◽  
Reproductive Age ◽  
University Hospital ◽  
Gardnerella Vaginalis ◽  
Genital Infections ◽  
Reproductive Age Women ◽  
Lower Genital Tract ◽  
Genital Tract Infections ◽  
Tract Infections

Introduction: Lower genital tract infections is one of the most common deseases among women in reproductive age and affects the quality of their lives. Objective: To assess the status of lower genital tract infections in reproductive age women and some factors related to vaginosis. Subjects and methods: Crosssectional study of 130 coming for examination at Hue University Hospital from August 2016 to December 2016. Results: The incidence of lower genital infections was 72.3%, with: vaginitis was 34%, vaginitis was 25.5%, vaginitis and cervical was 40.5%. Fungal infection is 20.2%, Gardnerella vaginalis infection is 33%, Parasitic infection is 0%. There is a signification relation between hygiene habits, inflammatory history and numbers of pregnancies with the rates of lower genital infections. There is not a signification relation between history of used contraceptive with lower genital infections. Conclusion: Lower genital tract infections are high percentage among women in reproductive age. There is a signification relation between hygiene habits, inflammatory history and numbers of pregnancies with the rates of lower genital infections. Key words: Women, reproductive age, lower genital tract infections

ETHIOLOGY OF PURULENT INFLAMMATORY PROCESSES IN THE GENITAL TRACT: SUSPICIONS OF CLINICISTS AND PROBLEMS OF LABORATORY CONFIRMATION

2020 ◽  
Vol 65 (5) ◽  
pp. 328-331
Author(s):  
D. A. Oborin ◽  
N. V. Nikolaeva ◽  
A. P. Godovalov ◽  
T. I. Karpunina
Keyword(s):  
Genital Tract ◽  
Culture Media ◽  
Clinical Manifestations ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Sufficient Information ◽  
Gonococcal Infection ◽  
Men And Women ◽  
Inflammatory Processes ◽  
Study Laboratory

Despite the long history of the study, laboratory diagnosis of gonococcal infection remains a complex task that does not have a clearly regulated effective solution. Aim of investigation was to assess the species diversity of the microbiota of the genital tract of men and women with suspected acute genital gonococcal infection (AGGI) using test systems of Russian manufacturers. A study of samples of the contents of the urethra of 69 men and posterior vaginal fornix fluids of 33 women of reproductive age with characteristic clinical manifestations and a presumptive diagnosis of AGGI was made. Cultivation was carried out using elective culture media with subsequent identification of strains by biochemical properties. Detection of DNA of Neisseria gonorrhoeae, Staphylococcus spp., Streptococcus spp., Enterobacteriaceae, Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. performed by PCR using Vektor-Best and InterLab Service kits (Russia). All patients were divided into groups according to the results of the bacteriological method and PCR. A metagenomic study of 16S ribosomal RNA samples was performed on the Illumina MiSeq platform using the MiSeq Reagent Kits v3 kit (600-Cycle Kit). Statistical analysis of the data was performed using criterion x2. As a result of a laboratory study, the presumptive clinical diagnosis of «AGGI» found its bacteriological confirmation in 35.3% of cases only, among which fragments of the N. gonorrhoeae genome were detected in 63.9% of the samples only. Moreover, a wide variety of microorganisms in the genital tract of both men and women was found in metagenomic analysis. However, this technique does not allow us to assess the viability of the detected bacteria, and the microflora spectrum is excessively wide. In addition, the high level of genetic polymorphism of different strains of N. gonorrhoeae complicates the interpretation of the results. Deciphering the composition of microbiota allows the use of InterLab Service kits. The decoding of the etiology of purulent-inflammatory processes in the genital tract, which presents serious difficulties, is greatly facilitated by the use of Russian kits for molecular genetic analysis, which, in our opinion, provide the necessary and sufficient information for practice.

The Quality of Life in the Women of Reproductive Age as Patients with Active Forms of Tuberculosis

10.12737/3314 ◽  
2014 ◽  
Vol 21 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Камаев ◽  
I. Kamaev ◽  
Русановская ◽  
G. Rusanovskaya ◽  
Шпрыков ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Pulmonary Tuberculosis ◽  
Life Quality ◽  
Reproductive Age ◽  
Control Group ◽  
Main Process ◽  
Women Of Reproductive Age ◽  
Healthy Women ◽  
Tuberculosis Disease

The authors assessed the quality of life of 263 women of reproductive age. The main group consisted of 139 women aged o18-44 as the patients with active pulmonary tuberculosis disease treated in 2011-2012, and the other group of comparison consisted of 124 healthy women of the same age. To assess the life quality the authors used Russian version of the SF-36 which included questions of medico- social nature. It was proved that all the life quality parameters of the women suffering from tuberculosis disease were reduced in comparison with the control group of the healthy women. The only exception was the pain syndrome for which the statistical significance wasn’t reached at all and the physical functioning was assessed at the lowest level. The life quality problem was identified almost in half of the women that proved by the obtained data. Analysis of quality of life of the women established that 25-34 years respondents had the highest reducing of the rates. The analyzed group of patients differed from the low level of the socio- economic independence relatively favorable structure of clinical tuberculosis forms but with a high frequency of tubercle bacilli discharging and detection of lung tissue destructing. Generally, women with pulmonary tuberculosis had numerous risk factors for disease and co-morbidities aggravating the main process. The unfavorable health and social hygienic characteristics of the analyzed group of patients contribute to late making a diagnosis and spreading the infection among the population, including the women of reproductive age. Therefore health care professionals need to make efforts to promote healthy lifestyle and prevention of tuberculosis with this contingent that will allow to prevent new cases of disease and to reduce morbidity. The aim of any disease treatment, including tuberculosis should be considered as acts to improve the life quality of patients in case of positive clinical dynamics. Therefore it is important to guide medical practice for the patients’ psychosomatic status correcting in the early stages of disease that will be able to solve health problems more successfully. Thereby, the authors think it is necessary to organize services; to open offices where patients could get psychosocial correction and rehabilitation, to train the specialists.

scholarly journals Virulence Attributes of Low-Virulence Organisms

1994 ◽  
Vol 2 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Bryan Larsen
Keyword(s):  
Virulence Factors ◽  
Genital Tract ◽  
Pathogenic Bacteria ◽  
Female Genital Tract ◽  
Gardnerella Vaginalis ◽  
Control Mechanisms ◽  
Normal Flora ◽  
Coordinate Expression ◽  
Lower Genital Tract ◽  
Virulence Properties

The vast majority of infections involving female pelvic structures arise from organisms that are members of the normal flora. In addition, exogenous organisms that invade through the lower genital tract must interact with organisms that are part of the host's flora. In contrast to the concept that the normal flora is entirely innocuous, recent research has begun to identify what appear to be virulence attributes among these ordinarily low-virulence organisms. Most of our understanding of virulence has been derived from highly virulent organisms, of which Neisseria gonorrhoeae provides an example of relevance to the female genital tract. A review of the virulence factors of the gonococcus is presented to serve as an example of the variety of virulence properties associated with pathogenic bacteria. Molecular biology has begun to clarify one of the important paradigms of pathogenic bacteriology—that bacteria change their expression of virulence properties in response to their location within a host or to the stage of infection. Thus, infection involves not only the possession of virulence factors, but also the carefully controlled use of those factors. Virulence is often controlled by the coordinate expression of many virulence-associated genes in response to one environmental signal. With regard to low- virulence organisms present in the female lower genital tract, we are beginning to identify some of their virulence attributes. Examples from the work of our laboratory include the hemolysin of Gardnerella vaginalis and an immunosuppressive mycotoxin produced by Candida albicans. Demonstrating the coordinate expression (or other control mechanisms) of virulence factors in these sometimes innocuous and sometimes inimical organisms represents the next frontier in the study of normal vaginal microbiology.

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