scholarly journals INFLUENCE OF CORN CONDENSED DISTILLERS SOLUBLES (CCDS) ON RATION DIGESTIBILITY. NITROGEN/ENERGY UTILIZATION AND RUMEN FLUID CHARACTERISTICS OF LACTATING DAIRY COWS

2021 ◽  
Vol 11 (2) ◽  
pp. 161-167
Author(s):  
A. B. I. Udedibie ◽  
L. E. Chase
Keyword(s):  
Dairy Cows ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Energy Utilization ◽  
Rumen Ph ◽  
Lactating Dairy Cows ◽  
Condensed Distillers Solubles ◽  
Balance Trials

Digestion and Balance trials were conducted with 18 lactating dairy cows in their 7-8 weeks of lactation using 7 day total collection to determine nitrogen and energy balance and digestibility of ration nutrients by lactating dairy cows fed three levels (0.8 and 16%) of corn condensed distillers solubles (CCDS). Cows were fed treatment rations for 4 weeks before collection. Following digestion and balance trials, rumen fluid samples were collected esophagially before feeding and 3 hours after feeding to determine the influence of CCDS on rumen pH, ammonia nitrogen and volatile fatty acids. Corn condensed distilers solubles significantly (P<0.05) improved the digestibility of the neutral detergent fiber and ether extract but depressed the digestibility of the ash. Apparent nitrogen retention was significantly (P <0.05) lower for animals on 8% CCDS ration. There were no significant differences in energy utilization among groups. Corn condensed distilers soluble did not significantly affect rumen pH, but depressed rumen ammonia nitrogen. On molar percentage basis, animals on the 8% CCDS ration had significantly (P< 0.05) higher acetate in the rumen 3 hours after feeding. Other VFA remained relatively unaffected by treatments.  

scholarly journals Alfalfa Protein Concentrate as a Less Ruminally Degradable Protein Source for High-Producing Dairy Cows

1997 ◽  
Vol 2 ◽  
pp. 31
Author(s):  
C.D. Lu ◽  
NA. Jorgensen
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
Protein Concentrate ◽  
Dietary Nitrogen

Ten multiparous Holstein cows were used in a 105-day continuous feeding trial to compare soybean meal (SBM) and alfalfa protein concentrate (APC) as supplemental protein sources for high-producing dairy cows. Dairy cows with an average milk production of 35.2 kg/day were paired and randomly assigned to one of the treatments. A double reversal design was employed With 35 days per period. The comparison between treatments was made during the last 21 days of each period. Experimental diets, containing 60% corn silage and 10% alfalfa hay, were formulated to be isonitrogenous and isoenergetic (crude protein. 15.6%; net energy tor lactation, 1.60 Mcal/kg DM). Fifty percent of dietary nitrogen was provided by test proteins. Animals were fed four times daily. Chromie oxide boluses, 50 g/day were used to estimate digestion coefficients. Ruminal protein degradability in viva (%) and in situ(%)were 56.8, 68.3 and 47.3. 35.3 for SBM and APC, respectively. Dry matter intake (kg/day) was 19.1 and 18.6 for cows fed SBM and APC. respectively. Rummal parameters cows fed SBM vs APC were, pH. 6.6 vs 6.7, ammonia nitrogen(mg/dI) 10.8 vs 6.2(p less than 0.01); total volatile fatty acids (mM), 86.3 VS 84.6(P LESS THAN 0.05), acetate (%) , 50.8 vs 64.9 (p less than 0.01); propionate dry matter , 65.3 vs 61.8; crude protein . 67.6 vs 61.1 (P less than 0.05 ) neutral detergent fiber , 51.1 vs 52.5 ; acid detergent fiber, 47.7 vs 49.0 . A slightly higher (P more than 0.05 ) amount of milk was produced by cows fed APC(28.6+_ 3.5 vs 28.2+- 3.8 kg/day). Each additional gramof undergradable intake protein provided by APC continued to a 5- gram increase in 4% fat-corrected milk production.

scholarly journals Effect of Dietary Fat on the Metabolism of Energy and Nitrogen, Serum Parameters, Rumen Fermentation, and Microbiota in twin Hu Male Lambs

2018 ◽  
Author(s):  
Wenjuan Li ◽  
Hui Tao ◽  
Naifeng Zhang ◽  
Tao Ma ◽  
Kaidong Deng ◽  
...  
Keyword(s):  
Dietary Fat ◽  
High Fat Diet ◽  
Volatile Fatty Acids ◽  
High Throughput Sequencing ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Energy Utilization ◽  
Sequencing Analysis ◽  
Ruminal Fermentation ◽  
High Fat

AbstractBackgroundFat is the main substance that provides energy to animals. However, the use of fat in twin Hu lambs has not been investigated. Thirty pairs of male twin lambs were examined to investigate the effects of dietary fat on the metabolism of energy and nitrogen, ruminal fermentation, and microbial communities. The twins are randomly allotted to two groups (high fat: HF, normal fat: NF). Two diets of equal protein and different fat levels. The metabolism test was made at 50-60 days of age. Nine pairs of twin lambs are slaughtered randomly, and the rumen fluid is collected at 60 days of age.ResultsThe initial body weight (BW) in the HF group did not differ from that of NF group (P > 0.05), but the final BW was tended to higher than that of NF group (0.05 < P < 0.1). The digestive energy (DE), metabolism energy (ME), DE/ME in the HF group tend to be higher than those in the NF group (0.05 < P < 0.1). Ruminal ammonia nitrogen (NH3-N) and the proportion of total volatile fatty acids (TVFA) are higher than that in the NF group (P < 0.05). A high throughput sequencing analysis reveals that there were no differences between the two groups in terms of the richness estimates and diversity indices (P > 0.05). The Proteobacteria and Fibrobacteres phyla were higher than that in NF group (P<0.05).ConclusionsThis study demonstrated that high fat diet before weaning can affect the abundance of several groups of rumen bacteria in rumen, such as significantly increasing phyla Proteobacteria and Fibrobacteres, and genera of Succinivibrio, Alloprevotella, and Saccharofermentans, but significantly decreasing genera of Clostridium IV, Dialister, Roseburia, and Butyrivibrio. And high fat diet improved the performance of lambs at weight gain, energy utilization, and had effect on VFA composition but no effects on serum enzymes and serum hormone.

Substitution and production responses when lactating dairy cows graze a white clover pasture supplemented with maize silage

1996 ◽  
Vol 36 (7) ◽  
pp. 771 ◽  
Author(s):  
CR Stockdale
Keyword(s):  
Dairy Cows ◽  
White Clover ◽  
Milk Fat ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Maize Silage ◽  
Lactating Dairy Cows ◽  
Clover Pasture ◽  
Friesian Cows

Friesian cows (16) in late lactation grazed pure white clover (Trifolium repens cv. Haifa) swards for 32 days in autumn and were supplemented with maize (Zea mays) silage. Four groups of 4 cows were offered either 19 or 39 kg dry matter (DM)/cow.day of white clover with either 0 or 4.4 kg DM/cow.day of maize silage. All cows were individually fed maize silage and grazed the pasture as individual groups according to treatment. When maize silage was fed, total intake increased, this occurring to a greater degree at the low pasture allowance. The level of substitution of silage for pasture was 0.14 and 0.40 kg DM reduction in pasture intake per kg DM of maize silage eaten at the low and high allowances, respectively. At the low pasture allowance, milk yields were 10.1 and 13.7 kg/cow. day when 0 and 4.4 kg DM/cow.day of maize silage were fed, respectively, and were 15.5 and 15.9 kg/cow.day at the high pasture allowance. Liveweight and body condition increased as plane of nutrition increased but there were no effects of feeding on milk fat or protein contents. Feeding maize silage had little effect on any rumen or faecal variable although there was generally less ammonia nitrogen in rumen fluid when cows were supplemented with maize silage.

scholarly journals Dandelion (Taraxacum mongolicum Hand.-Mazz.) Supplementation-Enhanced Rumen Fermentation through the Interaction between Ruminal Microbiome and Metabolome

2020 ◽  
Vol 9 (1) ◽  
pp. 83
Author(s):  
Yan Li ◽  
Mei Lv ◽  
Jiaqi Wang ◽  
Zhonghong Tian ◽  
Bo Yu ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Rumen Fluid ◽  
Basal Diet ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Pentose Phosphate ◽  
Metabolome Analysis ◽  
Bacterial Phyla ◽  
Ruminal Microorganisms ◽  
Lactating Dairy Cows

This study investigated the effects of dandelion on the ruminal metabolome and microbiome in lactating dairy cows. A total of 12 mid-lactation dairy cows were selected and randomly classified into two groups, supplementing dandelion with 0 (CON) and 200 g/d per cow (DAN) above basal diet, respectively. Rumen fluid samples were collected in the last week of the trial for microbiome and metabolome analysis. The results showed that supplementation of DAN increased the concentrations of ammonia nitrogen, acetate, and butyrate significantly. The rumen bacterial community was significantly changed in the DAN group, with Bacterioidetes, Firmicutes, and Proteobacteria being the main ruminal bacterial phyla. The abundance of Ruminococcaceae_NK4A214_group, UCG_005, and Christensenellaceae_R_7_group were relatively higher, whereas that of Erysipelotrichaceae_UCG_002 and Dialister were lower in the DAN than those in the CON. Metabolomics analysis showed that the content of d-glucose, serotonin, ribulose-5-phosphate, and d-glycerate were higher in the DAN group. These metabolites were enriched in the starch and sucrose metabolism, pentose phosphate pathway, tryptophan metabolism, and glycerolipid metabolism. The ribulose-5-phosphate and d-glycerate were correlated with Ruminococcaceae_NK4A214_group, UCG_005, and Christensenellaceae_R-7_group positively. This study demonstrated that the supplementation of dandelion impacts the ruminal microorganisms and metabolites in a way that rumen fermentation was enhanced in lactating dairy cows.

The effect of feeding maize silage 1 h or 9 h before the herbage meal on dry matter intake, milk production, nitrogen partitioning and rumen function of lactating dairy cows

2016 ◽  
Vol 56 (12) ◽  
pp. 2004 ◽  
Author(s):  
O. Al-Marashdeh ◽  
P. Gregorini ◽  
S. L. Greenwood ◽  
G. Edwards
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Body Condition Score ◽  
Nitrogen Partitioning ◽  
Maize Silage ◽  
Rumen Ph ◽  
Lactating Dairy Cows ◽  
Condition Score

The study was conducted to evaluate the effect of the timing of feeding maize silage before a short herbage meal on dry matter (DM) intake, milk production, nitrogen (N) partitioning and rumen function of lactating dairy cows. Ten lactating, pregnant, Friesian × Jersey rumen-fistulated dairy cows aged 4–5 years were blocked in pairs by milksolids (MS; sum of protein and fat) production (1.73 ± 0.097 kg MS/cow.day; mean ± s.d.), bodyweight (519 ± 24.7 kg), days in milk (163 ± 12.5), and body condition score (3.9 ± 0.21). Cows within pairs were randomly allocated to one of two treatments: (1) five cows were offered one-third of their estimated total DM intake (5 kg/cow.day) as maize silage after the afternoon milking at 1500 hours, ~1 h before herbage was provided (1BH); (2) five cows were offered one-third of their estimated total DM intake as maize silage after morning milking at 0700 hours, ~9 h before herbage was provided (9BH). All cows were offered two-thirds of their total estimated DM intake (11 kg/cow.day) over a period of 4 h from 1600 hours to 2000 hours as freshly cut perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens) in an indoor system. Cows were adapted to diets for 9 days (Days 1–9), followed by 5 days of measurement (Days 10–14). Maize silage DM intake did not differ between treatments averaging 5.1 kg DM/cow.day. Herbage DM intake was greater (P = 0.02) for 9BH (11.2 kg DM/cow.day) than 1BH (10.4 kg DM/cow.day). Milk yield (P = 0.001) and MS production (P = 0.08) were greater for 9BH than 1BH (18.8 vs 15.4 kg/cow.day and 1.63 vs 1.48 kg MS/cow.day, respectively). There was no effect of time of supplementation on N partitioned to milk, faeces, or urine. The purine derivatives to creatinine ratio, as an indication of microbial protein production, was greater (P = 0.04) for 9BH than 1BH. Daily mean rumen pH and the concentration of total rumen volatile fatty acids, acetate, butyrate, and ammonia N were not affected by treatment. However, the peak concentration of ruminal ammonia N was 27% lower (P < 0.05) for 9BH than 1BH. The concentration of non-esterified fatty acid (P = 0.03) and beta-hydroxy butyric acid (P = 0.01) were greater for 1BH than 9BH (0.14 vs 0.09 and 0.81 vs 0.71 mmol/L, respectively). These results indicate that under herbage feed restriction, altering the time of supplementation relative to the herbage meal can increase herbage DM intake and subsequent milk production.

scholarly journals Managing Ammonia Emissions from Dairy Cows by Amending Slurry with Alum or Zeolite or by Diet Modification

2001 ◽  
Vol 1 ◽  
pp. 860-865 ◽  
Author(s):  
John J. Meisinger ◽  
Alan M. Lefcourt ◽  
Jo Ann S. Van Kessel ◽  
Victor Wilkerson
Keyword(s):  
Dairy Cows ◽  
Nitrogen Sources ◽  
Neutral Detergent Fiber ◽  
Wind Tunnels ◽  
Conservation Strategies ◽  
Nh3 Volatilization ◽  
Available N ◽  
Lactating Dairy Cows ◽  
Potential Contributor ◽  
On Farm

Animal agriculture is a significant source of atmospheric ammonia. Ammonia (NH3) volatilization represents a loss of plant available N to the farmer and a potential contributor to eutrophication in low-nitrogen input ecosystems. This research evaluated on-farm slurry treatments of alum or zeolite and compared three diets for lactating dairy cows in their effectiveness to reduce NH3 emissions. NH3 emissions were compared using a group of mobile wind tunnels. The addition of 2.5% alum or 6.25% zeolite to barn-stored dairy slurry reduced NH3 volatilization by 60% and 55%, respectively, compared to untreated slurry. The alum conserved NH3 by acidifying the slurry to below pH 5, while the zeolite conserved ammonia by lowering the solution-phase nitrogen through cation exchange. The use of alum or zeolite also reduced soluble phosphorus in the slurry. NH3 loss from fresh manure collected from lactating dairy cows was not affected by three diets containing the same level of crude protein but differing in forage source (orchardgrass silage vs. alfalfa silage) or neutral detergent fiber (NDF) content (30% vs. 35% NDF). NH3 losses from the freshly excreted manures occurred very rapidly and included the urea component plus some unidentified labile organic nitrogen sources. NH3 conservation strategies for fresh manures will have to be active within the first few hours after excretion in order to be most effective. The use of alum or zeolites as an on-farm amendment to dairy slurry offers the potential for significantly reducing NH3 emissions.

Intraruminal administration ofMegasphaera elsdeniimodulated rumen fermentation profile in mid-lactation dairy cows

2011 ◽  
Vol 79 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Qendrim Zebeli ◽  
Sarah J Terrill ◽  
Alberto Mazzolari ◽  
Suzanna M Dunn ◽  
Wen Z Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Plasma Concentrations ◽  
Rumen Fermentation ◽  
Holstein Cows ◽  
Plasma Metabolites ◽  
Fermentation Profile

This study evaluated the effects of intraruminal administration ofMegasphaera elsdeniion ruminal fermentation patterns, the profile of plasma metabolites, and milk yield and composition of mid-lactation dairy cows. Eight primiparous, ruminally cannulated Holstein cows were arranged in a paired 2×2 crossover design. Cows were randomly assigned to one of two treatments: 1) intraruminal inoculation of 35 ml suspension per day ofM. elsdeniiATCC 25940 (MEGA), containing 108cfu/ml of bacteria, dissolved in 35 ml of saline (0·15m), or 2) carrier alone (35 ml saline; CTR). Both postprandial and preprandial rumen volatile fatty acids (VFA) and plasma metabolite measurements were analysed. Postprandial VFA patterns were affected the most, with butyrate (P<0·01) and valerate (P<0·01) proportions increasing, and acetate (P<0·01), isobutyrate (P=0·05) and isovalerate (P<0·01) decreasing in MEGA cows. Preprandial data measured at various days showed that MEGA dosage tended to increase the molar proportion of propionate (P=0·09) and lower the acetate to propionate ratio (P=0·07) in the rumen fluid. There was no effect of treatment on rumen pH and on the concentration of lactate in the rumen as well as on selected preprandial plasma metabolites. Postprandial plasma concentrations of cholesterol tended to increase (P=0·07) in MEGA cows compared with CTR. Concentrations of non-esterified fatty acids (NEFA) in the plasma were lower in MEGA cows after the morning feeding (P<0·01). Sampling hour also affected plasma NEFA in this study. Plasma β-hydroxybutyrate (BHBA) were not affected by the treatment (P>0·05); however, after the morning feeding BHBA concentration was increased in both groups of cows. Dry matter intake and milk yield and composition were not affected by treatment. In conclusion, results indicate thatM. elsdeniihas the potential to modulate the rumen fermentation profile in mid-lactation Holstein cows, but these effects were only slightly reflected in changes in plasma metabolites and milk composition.

The intake, digestibility and utilization of a low-quality roughage by Brahman cross, buffalo, banteng and Shorthorn steers

1979 ◽  
Vol 30 (2) ◽  
pp. 333 ◽  
Author(s):  
JB Moran ◽  
BW Norton ◽  
JV Nolan
Keyword(s):  
Feed Intake ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Lower Proportion ◽  
Energy Utilization ◽  
Residence Times ◽  
Fluid Flow Rate ◽  
Faecal Excretion ◽  
Voluntary Feed Intake ◽  
Nitrogen Phosphorus

The intake, digestibility and utilization of a low quality (0.8% nitrogen) sorghum hay was studied in Brahman cross, buffalo, banteng and Shorthorn steers. Comparisons of diet utilization were made after data were adjusted for differences in liveweight and feed intake. There were no significant differences (P < 0.05) between species in voluntary feed intake, faecal excretion of organic matter, cellulose and energy, and urinary excretion of nitrogen, phosphorus and energy. Utilization of water was least efficient in the buffaloes and most efficient in the banteng. The concentrations of ammonia and volatile fatty acids in the rumens of Brahman crosses, buffaloes and bantengs were similar, but the Brahman crosses had a higher proportion of butyric and a lower proportion of propionic acid than the other two species. The bantengs had a smaller rumen fluid volume and a slower rumen fluid flow rate but all three species had similar mean marker residence times in the rumen. The results from this experiment support the view that there are few differences between cattle species in their ability to digest and utilize a low quality roughage when comparisons are made between animals of similar liveweight and feed intake.

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