scholarly journals Effect of Dietary Fat on the Metabolism of Energy and Nitrogen, Serum Parameters, Rumen Fermentation, and Microbiota in twin Hu Male Lambs

2018 ◽  
Author(s):  
Wenjuan Li ◽  
Hui Tao ◽  
Naifeng Zhang ◽  
Tao Ma ◽  
Kaidong Deng ◽  
...  
Keyword(s):  
Dietary Fat ◽  
High Fat Diet ◽  
Volatile Fatty Acids ◽  
High Throughput Sequencing ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Energy Utilization ◽  
Sequencing Analysis ◽  
Ruminal Fermentation ◽  
High Fat

AbstractBackgroundFat is the main substance that provides energy to animals. However, the use of fat in twin Hu lambs has not been investigated. Thirty pairs of male twin lambs were examined to investigate the effects of dietary fat on the metabolism of energy and nitrogen, ruminal fermentation, and microbial communities. The twins are randomly allotted to two groups (high fat: HF, normal fat: NF). Two diets of equal protein and different fat levels. The metabolism test was made at 50-60 days of age. Nine pairs of twin lambs are slaughtered randomly, and the rumen fluid is collected at 60 days of age.ResultsThe initial body weight (BW) in the HF group did not differ from that of NF group (P > 0.05), but the final BW was tended to higher than that of NF group (0.05 < P < 0.1). The digestive energy (DE), metabolism energy (ME), DE/ME in the HF group tend to be higher than those in the NF group (0.05 < P < 0.1). Ruminal ammonia nitrogen (NH3-N) and the proportion of total volatile fatty acids (TVFA) are higher than that in the NF group (P < 0.05). A high throughput sequencing analysis reveals that there were no differences between the two groups in terms of the richness estimates and diversity indices (P > 0.05). The Proteobacteria and Fibrobacteres phyla were higher than that in NF group (P<0.05).ConclusionsThis study demonstrated that high fat diet before weaning can affect the abundance of several groups of rumen bacteria in rumen, such as significantly increasing phyla Proteobacteria and Fibrobacteres, and genera of Succinivibrio, Alloprevotella, and Saccharofermentans, but significantly decreasing genera of Clostridium IV, Dialister, Roseburia, and Butyrivibrio. And high fat diet improved the performance of lambs at weight gain, energy utilization, and had effect on VFA composition but no effects on serum enzymes and serum hormone.

Sesamin Modulation of Intestinal Microflora in Rats on High-Fat Diet

2020 ◽  
Vol 19 (3) ◽  
pp. 312-316
Author(s):  
Pan Liu ◽  
Yu Deng ◽  
Mengxi Wang ◽  
Lingyan Kong ◽  
Na Xu ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Intestinal Microflora ◽  
High Throughput Sequencing ◽  
Intestinal Flora ◽  
Short Chain Fatty Acids ◽  
Sequencing Analysis ◽  
Anaerobic Culture ◽  
High Fat ◽  
E Coli

The present study aimed to investigate the effects of sesamin on intestinal flora in rats on high-fat diet by in vitro animal fecal anaerobic culture system. Results showed that treatment with sesamin increased the abundance of Lactobacillus acidophilus and inhibited the growth of E. coli. High throughput sequencing analysis showed that treatment with sesamin increased the abundance of probiotics such as Lactobacillus and Flavonifractor, and decreased the abundance of harmful bacteria such as Bacteroides, Enterococcus, Clostridium_XlVa, and Parabacteroides. Moreover, treatment with sesamin increased the concentration of total short chain fatty acids (acetic acid and isovaleric acid). In conclusion, sesamin intervention improved the composition and abundance of intestinal microorganisms of high fat diet rats, which would be beneficial to intestinal health.

Effect of increasing level of spineless cactus (Opuntia ficus indica var. inermis) on intake and digestion by sheep given straw-based diets

1996 ◽  
Vol 62 (2) ◽  
pp. 293-299 ◽  
Author(s):  
H. Ben Salem ◽  
A. Nefzaoui ◽  
H. Abdouli ◽  
E. R. Ørskov
Keyword(s):  
Drinking Water ◽  
Wheat Straw ◽  
Water Consumption ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Cellulolytic Activity ◽  
Ruminal Fermentation ◽  
Opuntia Ficus Indica ◽  
Vitamin Mixture

AbstractThe effects of spineless cactus (Opuntia ficus indica var. inermis) supply on digestion of wheat straw was studied n rumen cannulated sheep. In addition to urea (10 g) and mineral and vitamin mixture (30 g), the animals — received wheat straw alone or supplemented with graded levels of spineless cactus (150, 300, 450 or 600 g dry natter (DM) per head per day). The diets were studied in five successive 25-day periods from February to June 992 in Tunisia. "Water consumption, food intake, digestibility, diurnal variations of ruminal fermentation (pH, mmonia nitrogen, volatile fatty acids, protozoal concentrations in rumen fluid) and cellulolytic activity in the umen were measured.Spineless cactus had high contents of ash (260 g/kg DM) and water (926 g/kg fresh weight) and low neutral-detergent fibre content (185 g NDF per kg DM). Crude protein (CP) content of spineless cactus was 2-5 times reater than that of wheat straw (58 v. 23 g/kg DM, respectively). Drinking water consumption was substantially educed (P < 0·001) as the level of spineless cactus increased. When spineless cactus supply exceeded 300 g DM peray, sheep consumed practically no drinking water. The voluntary intake of straw increased significantly with vineless cactus level. Total diet apparent digesibilities of DM, organic matter and CP tended to increase with mneless cactus supply. Such increase was significant only with the 450 and 600 g levels of cactus. NDF and acidetergent-fibre apparent digestibilities were not affected by spineless cactus supply. Addition of spineless cactus up i 300 g DM significantly increased ruminal ammonia nitrogen (P< 0·001) but there was no additional effect with irther supplementation. When sheep were supplemented with spineless cactus, total rumen volatile fatty acid icreased and acetate: propionate ratio decreased significantly. Moreover, spineless cactus supply increased total wtozoa number and reduced significantly rumen cellulolytic activity measured as DM and NDF disappearance of heat straw from incubated nylon bags.

scholarly journals INFLUENCE OF CORN CONDENSED DISTILLERS SOLUBLES (CCDS) ON RATION DIGESTIBILITY. NITROGEN/ENERGY UTILIZATION AND RUMEN FLUID CHARACTERISTICS OF LACTATING DAIRY COWS

2021 ◽  
Vol 11 (2) ◽  
pp. 161-167
Author(s):  
A. B. I. Udedibie ◽  
L. E. Chase
Keyword(s):  
Dairy Cows ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Energy Utilization ◽  
Rumen Ph ◽  
Lactating Dairy Cows ◽  
Condensed Distillers Solubles ◽  
Balance Trials

Digestion and Balance trials were conducted with 18 lactating dairy cows in their 7-8 weeks of lactation using 7 day total collection to determine nitrogen and energy balance and digestibility of ration nutrients by lactating dairy cows fed three levels (0.8 and 16%) of corn condensed distillers solubles (CCDS). Cows were fed treatment rations for 4 weeks before collection. Following digestion and balance trials, rumen fluid samples were collected esophagially before feeding and 3 hours after feeding to determine the influence of CCDS on rumen pH, ammonia nitrogen and volatile fatty acids. Corn condensed distilers solubles significantly (P<0.05) improved the digestibility of the neutral detergent fiber and ether extract but depressed the digestibility of the ash. Apparent nitrogen retention was significantly (P <0.05) lower for animals on 8% CCDS ration. There were no significant differences in energy utilization among groups. Corn condensed distilers soluble did not significantly affect rumen pH, but depressed rumen ammonia nitrogen. On molar percentage basis, animals on the 8% CCDS ration had significantly (P< 0.05) higher acetate in the rumen 3 hours after feeding. Other VFA remained relatively unaffected by treatments.  

scholarly journals Regulation of plasma leptin in mice: influence of age, high-fat diet, and fasting

1997 ◽  
Vol 273 (1) ◽  
pp. R113-R120 ◽  
Author(s):  
B. Ahren ◽  
S. Mansson ◽  
R. L. Gingerich ◽  
P. J. Havel
Keyword(s):  
Body Weight ◽  
Dietary Fat ◽  
High Fat Diet ◽  
Plasma Insulin ◽  
Plasma Concentrations ◽  
Age Groups ◽  
Fat Content ◽  
High Fat ◽  
Background Strain ◽  
Plasma Leptin

Mechanisms regulating circulating leptin are incompletely understood. We developed a radioimmunoassay for mouse leptin to examine the influence of age, dietary fat content, and fasting on plasma concentrations of leptin in the background strain for the ob/ob mouse, the C57BL/6J mouse. Plasma leptin increased with age [5.3 +/- 0.6 ng/ml at 2 mo (n = 23) vs. 14.2 +/- 1.6 ng/ml at 11 mo (n = 15), P < 0.001]. Across all age groups (2-11 mo, n = 160), log plasma leptin correlated with body weight (r = 0.68, P < 0.0001), plasma insulin (r = 0.38, P < 0.001), and amount of intra-abdominal fat (r = 0.90, P < 0.001), as revealed by magnetic resonance imaging. Plasma leptin was increased by a high-fat diet (58% fat for 10 mo) and reduced by fasting for 48 h. The reduction of plasma leptin was correlated with the reduction of plasma insulin (r = 0.43, P = 0.012) but not with the initial body weight or the change in body weight. Moreover, the reduction in plasma leptin by fasting was impaired by high-fat diet. Thus plasma leptin in C57BL/6J mice 1) increases with age or a high-fat diet; 2) correlates with body weight, fat content, and plasma insulin; and 3) is reduced during fasting by an action inhibited by high-fat diet and related to changes of plasma insulin.

Impact of graded maternal dietary fat content on offspring susceptibility to high‐fat diet in mice

Obesity ◽  
2021 ◽  
Vol 29 (12) ◽  
pp. 2055-2067
Author(s):  
Yi Huang ◽  
Jazmin Osorio Mendoza ◽  
Min Li ◽  
Zengguang Jin ◽  
Baoguo Li ◽  
...  
Keyword(s):  
Dietary Fat ◽  
High Fat Diet ◽  
Fat Content ◽  
High Fat

scholarly journals Concentrate containing calcium salts of fatty acids rich in polyunsaturated fatty acids can change rumen fermentation in grazing goats

2018 ◽  
Vol 39 (6) ◽  
pp. 2621
Author(s):  
Ludmila Couto Gomes ◽  
Claudete Regina Alcalde ◽  
Julio Cesar Damasceno ◽  
Luiz Paulo Rigolon ◽  
Ana Paula Silva Possamai ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Calcium Salts ◽  
Linear Effect ◽  
Concentrate Supplementation ◽  
Ruminal Ph

Feeding goats with calcium salts of fatty acids (CSFA) can supply ruminants with lipids, with minimal effects on ruminal fermentation and fiber digestibility. However, there is a shortage of information on the effect of CSFA on characteristics of rumen fermentation in grassland goats. Thus, the present study aimed to assess the addition of CSFA to concentrate on the parameters of rumen fermentation of grazing goats. Five rumen cannulated goats were distributed in a Latin square 5x5 design (treatments: 0%, 1.5%, 3.0%, 4.5% and 6.0% CSFA. The pH, ammonia N and volatile fatty acids (VFA) content were analyzed in the ruminal fluid at 0, 2, 4, 6 and 8 hours after concentrate supplementation. The pH and ammonia N concentration showed a linear effect with the addition of CSFA. There was no effect observed for the VFA molar concentration after grazing goats were fed with the experimental diet. In conclusion, further research is needed to investigate the addition of CSFA to goat diets because there is evidence that CSFA increases ruminal pH and decreases excess ruminal ammonia without changing the VFA concentration in the rumen fluid.

scholarly journals Impact of Dietary Fat on the Progression of Liver Fibrosis: Lessons from Animal and Cell Studies

2021 ◽  
Vol 22 (19) ◽  
pp. 10303
Author(s):  
Fangping Jia ◽  
Xiao Hu ◽  
Takefumi Kimura ◽  
Naoki Tanaka
Keyword(s):  
Fatty Acid ◽  
Hepatitis C ◽  
Liver Fibrosis ◽  
Dietary Fat ◽  
High Fat Diet ◽  
Core Gene ◽  
High Fat ◽  
Virus Core ◽  
Liver Fibrogenesis ◽  
Isocaloric Diets

Previous studies have revealed that a high-fat diet is one of the key contributors to the progression of liver fibrosis, and increasing studies are devoted to analyzing the different influences of diverse fat sources on the progression of non-alcoholic steatohepatitis. When we treated three types of isocaloric diets that are rich in cholesterol, saturated fatty acid (SFA) and trans fatty acid (TFA) with hepatitis C virus core gene transgenic mice that spontaneously developed hepatic steatosis without apparent fibrosis, TFA and cholesterol-rich diet, but not SFA-rich diet, displayed distinct hepatic fibrosis. This review summarizes the recent advances in animal and cell studies regarding the effects of these three types of fat on liver fibrogenesis.

scholarly journals Using glycerin with chitosan extracted from shrimp residue to enhance rumen fermentation and feed use in native Thai bulls

2021 ◽  
pp. 1158-1164
Author(s):  
Anuthida Seankamsorn ◽  
Anusorn Cherdthong ◽  
Sarong So ◽  
Metha Wanapat
Keyword(s):  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Crude Glycerin ◽  
Ruminal Fermentation ◽  
Design Factor ◽  
Ch4 Production ◽  
High Level

Background and Aim: Crude glycerin is changed to propionate in the rumen, while chitosan can be used as a feed supplement to increase propionic acid concentration and decrease methane (CH4) production. We hypothesized that supplementation with a combination of a high level of crude glycerin with chitosan could have a beneficial effect on ruminal fermentation and mitigate CH4 production. This study aimed to explore the combined effects of crude glycerin and chitosan supplementation on nutrient digestibility, rumen fermentation, and CH4 calculation in native Thai bulls. Materials and Methods: Four 2-year-old native Thai bulls, weighing 150±20 kg, were kept in a 2×2 factorial arrangement in a 4×4 Latin square design. Factor A represented the incorporation of crude glycerin at 10.5% and 21% of the dry matter (DM) of a total mixed ration (TMR), and factor B represented the supplementation of chitosan at 1% and 2% DM of a TMR. Results: Increasing levels of crude glycerin at 21% decreased DM intake by 0.62 kg/day compared with 10.5% crude glycerin (p<0.05), whereas nutrient digestibility did not change (p>0.05). The incorporated crude glycerin and supplemented chitosan levels did not affect the pH, temperature, concentrations of ammonia-nitrogen, microbial population, and blood urea nitrogen (p>0.05). Supplemented chitosan and incorporated crude glycerin did not show any interaction effects on the molar portions and total volatile fatty acids (VFAs), except estimated CH4. Increasing the incorporated crude glycerin levels increased propionate and decreased the ratio of acetate to propionate ratio, whereas levels of butyrate, acetate, and total VFAs were unchanged. The combination of crude glycerin at 21% in the TMR with chitosan at 2% reduced CH4 estimation by 5.08% compared with the other feed treatment. Conclusion: Increasing incorporated crude glycerin levels in a TMR significantly elevated the propionate concentration, whereas combining 21% crude glycerin in the TMR diet with 2% chitosan supplementation could depress CH4 estimation more effectively than adding one of these supplements alone.

The intake, digestibility and utilization of a low-quality roughage by Brahman cross, buffalo, banteng and Shorthorn steers

1979 ◽  
Vol 30 (2) ◽  
pp. 333 ◽  
Author(s):  
JB Moran ◽  
BW Norton ◽  
JV Nolan
Keyword(s):  
Feed Intake ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Lower Proportion ◽  
Energy Utilization ◽  
Residence Times ◽  
Fluid Flow Rate ◽  
Faecal Excretion ◽  
Voluntary Feed Intake ◽  
Nitrogen Phosphorus

The intake, digestibility and utilization of a low quality (0.8% nitrogen) sorghum hay was studied in Brahman cross, buffalo, banteng and Shorthorn steers. Comparisons of diet utilization were made after data were adjusted for differences in liveweight and feed intake. There were no significant differences (P < 0.05) between species in voluntary feed intake, faecal excretion of organic matter, cellulose and energy, and urinary excretion of nitrogen, phosphorus and energy. Utilization of water was least efficient in the buffaloes and most efficient in the banteng. The concentrations of ammonia and volatile fatty acids in the rumens of Brahman crosses, buffaloes and bantengs were similar, but the Brahman crosses had a higher proportion of butyric and a lower proportion of propionic acid than the other two species. The bantengs had a smaller rumen fluid volume and a slower rumen fluid flow rate but all three species had similar mean marker residence times in the rumen. The results from this experiment support the view that there are few differences between cattle species in their ability to digest and utilize a low quality roughage when comparisons are made between animals of similar liveweight and feed intake.

scholarly journals Effects of dietary fat quantity and composition on fasting and postprandial levels of coagulation factor VII and serum choline-containing phospholipids

2003 ◽  
Vol 90 (2) ◽  
pp. 329-336 ◽  
Author(s):  
Anja Schou Lindman ◽  
Hanne Müller ◽  
Ingebjørg Seljeflot ◽  
Hans Prydz ◽  
Marit Veierød ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dietary Fat ◽  
High Fat Diet ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Coagulation Factor ◽  
Factor Vii ◽  
High Fat ◽  
Coagulation Factor Vii ◽  
Low Fat Diet

Dietary fat influences plasma levels of coagulation factor VII (FVII) and serum phospholipids (PL). It is, however, unknown if the fat-mediated changes in FVII are linked to PL. The present study aimed to investigate the effects of dietary fat on fasting and postprandial levels of activated FVII (FVIIa), FVII coagulant activity (FVIIc), FVII protein (FVIIag) and choline-containing PL (PC). In a randomized single-blinded crossover-designed study a high-fat diet (HSAFA), a low-fat diet (LSAFA), both rich in saturated fatty acids, and a high-fat diet rich in unsaturated fatty acids (HUFA) were consumed for 3 weeks. Twenty-five healthy females, in which postprandial responses were studied in a subset of twelve, were included. The HSAFA diet resulted in higher levels of fasting FVIIa and PC compared with the LSAFA and the HUFA diets (all comparisonsP≤0·01). The fasting PC levels after the LSAFA diet were also higher than after the HUFA diet (P<0·001). Postprandial levels of FVIIa and PC were highest on the HSAFA diet and different from LSAFA and HUFA (all comparisonsP≤0·05). Postprandial FVIIa was higher on the HUFA compared with the LSAFA diet (P<0·03), whereas the HUFA diet resulted in lower postprandial levels of PC than the LSAFA diet (P<0·001). Significant correlations between fasting levels of PC and FVIIc were found on all diets, whereas FVIIag was correlated to PC on the HSAFA and HUFA diet. The present results indicate that dietary fat, both quality and quantity, influences fasting and postprandial levels of FVIIa and PC. Although significant associations between fasting FVII and PC levels were found, our results do not support the assumption that postprandial FVII activation is linked to serum PC.

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