scholarly journals Alfalfa Protein Concentrate as a Less Ruminally Degradable Protein Source for High-Producing Dairy Cows

Author(s):  
C.D. Lu ◽  
NA. Jorgensen

Ten multiparous Holstein cows were used in a 105-day continuous feeding trial to compare soybean meal (SBM) and alfalfa protein concentrate (APC) as supplemental protein sources for high-producing dairy cows. Dairy cows with an average milk production of 35.2 kg/day were paired and randomly assigned to one of the treatments. A double reversal design was employed With 35 days per period. The comparison between treatments was made during the last 21 days of each period. Experimental diets, containing 60% corn silage and 10% alfalfa hay, were formulated to be isonitrogenous and isoenergetic (crude protein. 15.6%; net energy tor lactation, 1.60 Mcal/kg DM). Fifty percent of dietary nitrogen was provided by test proteins. Animals were fed four times daily. Chromie oxide boluses, 50 g/day were used to estimate digestion coefficients. Ruminal protein degradability in viva (%) and in situ(%)were 56.8, 68.3 and 47.3. 35.3 for SBM and APC, respectively. Dry matter intake (kg/day) was 19.1 and 18.6 for cows fed SBM and APC. respectively. Rummal parameters cows fed SBM vs APC were, pH. 6.6 vs 6.7, ammonia nitrogen(mg/dI) 10.8 vs 6.2(p less than 0.01); total volatile fatty acids (mM), 86.3 VS 84.6(P LESS THAN 0.05), acetate (%) , 50.8 vs 64.9 (p less than 0.01); propionate dry matter , 65.3 vs 61.8; crude protein . 67.6 vs 61.1 (P less than 0.05 ) neutral detergent fiber , 51.1 vs 52.5 ; acid detergent fiber, 47.7 vs 49.0 . A slightly higher (P more than 0.05 ) amount of milk was produced by cows fed APC(28.6+_ 3.5 vs 28.2+- 3.8 kg/day). Each additional gramof undergradable intake protein provided by APC continued to a 5- gram increase in 4% fat-corrected milk production.

Krmiva ◽  
2020 ◽  
Vol 61 (1) ◽  
pp. 3-10
Author(s):  
Worku Alamayehu ◽  
Hedvig Fébel ◽  
Szilvia Orosz ◽  
Tamȧs Tóth ◽  
György Bȧzȧr ◽  
...  

The interest in new alternative forages in Europe has increased in recent years. The nutritional composition and fermentation characteristics during different stages of ensiling were studied with Italian ryegrass (Lolium multiflorum Lam.) and winter cereal mixtures. The trial was carried out on a large-scale farm Galgamenti Agricultural Limited Company, Tura, Hungary. Two different forage mixes were studied: Mixture A (three types of Italian ryegrass 40% + two types of triticale 20%+ two types of oats 20%+ wheat 15%+ barley 5%) and Mixture B (three types of Italian ryegrass 55% + two types of winter oats 45%). Experimental field was 30.600 m 2 by the treatments, respectively. The two different forage mixes were sown on 11th September 2017 (Mixture A: 75 kg seed/ha; Mixture B: 75 kg seed/ha) with depth of 2-5 cm. Plant protection treatment was not applied during the growing season. Cutting was carried out in heading stage of triticale by hand at 10 cm stubble height. The fresh Mixture A (dry matter 189 g/kg; crude protein: 161 g/kg DM; NDF: 485 g/kg DM) and the fresh Mixture B (dry matter 195 g/kg; crude protein: 159 g/kg DM; NDF: 519 g/kg DM) were wilted to 28-32% DM (24h) without any movement on the windrow. The wilted forage was picked up by hand and chopped by a forage harvester (John Deere 7300) on concrete surface with theoretical chop length of 9 mm (weight: 800 kg). Wilted and chopped material of 510 g were packed by hand into a glass jars (0.00072 m3 volume, n=5, total no. of minisilos = 15). Five laboratory silos per mixtures were opened on 7, 14 and 90 days after ensiling. Dry matter (DM), crude protein (CP), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), ether extract (EE), ash, and total sugar of all treatments were determined. Additionally, pH and the concentration of ammonia-N, volatile fatty acids were measured in the ensiled mixtures. At the end of 90 days of ensiling in both mixture silages, there were significant differences (p<0.05) in all nutrient contents except for ADF in Mixture A and CP, ash and CF in Mixture B which was not affected by fermentation duration. Ensiling caused a significant decrease in pH (p<0.05), due to the production of lactic acid and succeeded to achieve lactic acid type fermentation. Values for ammonia-N, ethanol and acetic acid, butyric acid were all low. These results indicated that the fermentation quality of Italian ryegrass and winter cereal silages underwent rapid fermentation and were well-preserved.


1999 ◽  
Vol 39 (8) ◽  
pp. 923 ◽  
Author(s):  
P. J. Moate ◽  
D. E. Dalley ◽  
J. R. Roche ◽  
C. Grainger

Summary. The effect of herbage allowance (20, 30, 40, 50, 60 and 70 kg DM/cow. day) on the consumption of nutrients from herbage and milk production by cows in early lactation, was examined. The experiment was conducted on rainfed perennial ryegrass pastures in September and October 1997 in south-eastern Victoria, Australia. The herbage on offer comprised 64% perennial ryegrass, 21% other grasses, 1% white clover, 5% weeds and 9% dead material on a dry matter (DM) basis. The average pregrazing herbage height was 13 cm, at an estimated pregrazing herbage mass of 3.6 t DM/ha. The herbage on offer was of high quality containing 11.6 MJ metabolisable energy/kg DM, 202 g crude protein/kg DM and 525 g neutral detergent fibre/kg DM. Concentrations of calcium, magnesium, sodium, potassium, phosphorus, sulfur and chloride were 4.4, 2.2, 4.4, 31.2, 3.5, 2.7 and 11.4 g/kg DM, respectively. As daily herbage allowance per cow increased, dry matter intake increased curvilinearly (P<0.01) from 11.2 to 18.7 kg DM/cow. day. This was associated with a decrease in utilisation of herbage from 54 to 26% and an increase in milk production from 25.9 to 29.1 kg/cow. day. The cows on all treatments grazed for less than 8.7 h/day. The increase in intake was achieved by an increase in the rate of herbage intake from 1.5 to 2.2 kg DM/h for herbage allowances of 20 and 70 kg/cow.day, respectively. Irrespective of herbage allowance, cows selected a diet that was approximately 10% higher in in vitro dry matter digestibility and 30% higher in crude protein than that in the herbage on offer. The neutral detergent fibre content of the herbage selected was lower (P<0.05) than that on offer. The herbage consumed contained more (P<0.05) magnesium, potassium and sulfur, the same amount of calcium and phosphorus and less (P<0.05) sodium and chloride than the herbage on offer. For rainfed perennial pastures in spring, herbage allowance is an important factor in determining voluntary feed intake and production of dairy cows. To achieve 30 L from herbage, without supplementation, high herbage allowances are required. The increase in herbage intake, with increasing herbage allowance, resulted from an increase in rate of dry matter intake and not an increase in grazing time. No relationship was evident between herbage allowance and the selection differentials for in vitro dry matter digestibility, crude protein and neutral detergent fibre. Selection differentials for rainfed perennial pastures in spring are similar to those reported for irrigated perennial pastures in northern Victoria in spring and autumn. When determining nutrient requirements it is important to consider the interaction between herbage intake and nutrient concentration in the herbage.


2021 ◽  
Vol 42 (2) ◽  
pp. 795-808
Author(s):  
Ernestina Ribeiro Santos Neta ◽  
◽  
Daiany Iris Gomes ◽  
Luis Rennan Sampaio Oliveira ◽  
Rafael Mezzomo ◽  
...  

This study evaluated the use of babassu cake as a replacement for soybean meal and pineapple byproduct silage as roughage in the diets of feedlot sheep. Ten rumen-fistulated sheep were used and distributed in an experimental design consisting of two 5 x 5 Latin squares. Babassu cake replaced soybean meal at four concentration levels (0, 26.66, 53.33, and 80%), and pineapple byproduct silage was used as roughage. A treatment was also evaluated in which elephant grass silage was used as roughage and corn and soybean meal were used as the concentrate. No effect of babassu cake as a replacement for soybean meal on dry matter intake, neutral detergent fiber, or crude protein was observed. Higher babassu cake replacement levels in the diets resulted in linear reductions in the digestibility of the dry matter, neutral detergent fiber, organic matter, nonfiber carbohydrates and total carbohydrates. For the digestibility of the crude protein, no effect was observed after the addition of babassu cake. The replacement levels of the babassu cake had no effect on the intake and retention of the daily nitrogen, ammonia nitrogen and ruminal pH. Babassu cake could replace soybean meal at concentrations of up to 80% when the roughage source was pineapple byproduct silage. Compared with elephant grass silage, pineapple byproduct silage improves nutrient intake and digestibility and nitrogen intake, retention, and absorption without compromising the ruminal pH or ammonia nitrogen of feedlot sheep.


2011 ◽  
Vol 27 (4) ◽  
pp. 1563-1569
Author(s):  
J. Markovic ◽  
R. Strbanovic ◽  
D. Terzic ◽  
R. Stanisavljevic ◽  
D. Djokic ◽  
...  

The objective of this study was to investigate the changes that take place in nutrient values of red clover (Trifolium pratense L.) cv K-27 at different stages of growth in the second and the third cut. The samples from different stages of growth: mid-bud stage, around 60% flowering and full flowering were investigated for crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), digestible dry matter (DDM), dry matter intake (DMI), relative feed value (RFV), total digestible nutrients (TDN), net energy-lactation (NEl), net energy-maintenance (NEm) and net energy-gain (NEg) content. Higher content of crude protein was found in the third cut (262.1 g kg-1 of DM) than in the second cut (260.8 g kg-1 of DM) in the first stage of development. TDN, DDM, DMI, RFV, NEl, NEm and NEg were calculated according to the appropriate equations adapted from common formulas for forages. The stage of plant development has a significant influence on the chemical composition and the relative feed value of red clover. The achieved results show that adequate maturity stage for cutting might be at mid-bloom stage, when crude protein content, NEl, NEm and NEg content are high.


2014 ◽  
Vol 66 (5) ◽  
pp. 1487-1494
Author(s):  
D.C. Abreu ◽  
R.P. Lana ◽  
A.S. Oliveira ◽  
F.A. Barbosa ◽  
F.L. Andrade ◽  
...  

The effect of using conventional urea (CU) or slow release urea (SRU) was evaluated by replacing soybean meal (SBM) in concentrated supplements in levels of 2, 4 or 6% (dry matter basis) on productive performance of crossbred Holstein x Zebu lactating dairy cows (499±61kg body weight and 167 days of lactation) grazing on elephant grass (11.5% CP and 60% NDF) under rotational grazing during the rainy season. A supplement control (no urea) was used containing SBM as a protein source. A total of 21 cows were distributed an incomplete randomized block design with three periods of 21 days each (14 days of adaptation and seven days of collection). The animals entered the paddocks with a pasture height of 110-120cm and left when the grass reached the height of 40-50cm. The concentrated isonitrogenous supplements (24% crude protein, dry matter basis) were provided in the amount of 3.2kg/cow/day (fed basis). There was no effect (P>0.05) on source of crude protein (SBM vs source NPN), source NPN, level of NPN, interaction between source NPN and level of NPN on milk production (10.0kg/day), fat milk production corrected to 3.5% (10.7kg/day), levels of fat (4.01%), protein (3.66%), lactose (4.16%), total solids (12.86%) and non-fat solids (8.60%) in milk. The replacement of CU by SRU does not promote improvement in the productive performance of crossbred dairy cows grazing on elephant grass during the rainy season. Urea (CU or SRU) can be included in up to 6% of the DM concentrated supplements, replacing SBM, without affecting the productive performance of crossbred cows (Holstein x Zebu) in pasture during the rainy season.


2011 ◽  
Vol 78 (4) ◽  
pp. 391-395 ◽  
Author(s):  
Cristiano Côrtes ◽  
Daniele da Silva-Kazama ◽  
Ricardo Kazama ◽  
Chaouki Benchaar ◽  
Lucia M Zeoula ◽  
...  

Flaxseed hull, a co-product obtained from flax processing, is a rich source of n−3 fatty acids but there is little information on digestibility of its nutrients by dairy cows. Four rumen-cannulated multiparous Holstein cows averaging 665±21 kg of body weight and 190±5 d in milk at the beginning of the experiment were assigned to a 4×4 Latin square design with four 28-d experimental periods to determine the effects of feeding monensin and flaxseed hulls on total tract apparent digestibility of nutrients and fatty acids. The four treatments were: (1) diet CO: control with neither flaxseed hulls nor monensin added; (2) diet FH containing 19·8 g flaxseed hulls/100 g dry matter (DM); (3) diet MO with 16 mg monensin/kg DM; (4) diet HM containing 19·8 g flaxseed hulls/100 g DM and 16 mg monensin/kg DM. Diets provided similar amounts of protein and net energy of lactation. Digestibility of crude protein was higher for diets containing flaxseed hulls and for diets supplemented with monensin. Flaxseed hulls supplementation decreased digestibility of acid and neutral detergent fibre. Significantly higher digestibility of ether extract and individual fatty acids was observed for treatments with flaxseed hulls compared with treatments without flaxseed hulls. A combination of flaxseed hulls and monensin did not result in better fatty acid digestibility than when feeding only flaxseed hulls.


2010 ◽  
Vol 39 (12) ◽  
pp. 2611-2616 ◽  
Author(s):  
Anderson de Moura Zanine ◽  
Edson Mauro Santos ◽  
João Ricardo Rebouças Dórea ◽  
Paulo Alfredo de Santana Dantas ◽  
Thiago Carvalho da Silva ◽  
...  

The objective of this experiment was to evaluate the effects of adding cassava scrapings on gas and effluent losses, dry matter recovery, pH, contents of N-NH3, organic acids and volatile fatty acids and the bromatological composition of elephant grass silages. It was used a randomized complete design, with four levels of cassava scrapings (0, 7, 15 or 30% natural matter) each one with four replications per level. The grass was cut at 50 days of regrowth and ensiled in 15-L silos, equipped with a Bunsen valve to allow gas outflow. The gas losses decreased quadratically with the addition of cassava scrapings, whereas effluent losses decreased linearly. Dry matter recovery increased quadratically with the addition of cassava scrapings. Dry matter (DM) concentration increased but crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose (HEM) decreased linearly with the addition of cassava scrapings. The pH value and lactic acid concentration increased quadratically with the addition of cassava scrapings. Contents of N-NH3 and butyric acid decreased quadratically with the addition of cassava scrapings, whereas acetic acid content decreased linearly. Addition of cassava scrapings reduced gas and effluent losses and improved the fermentation profile of elephant grass silages and the level of 7% already ensures this improvement.


1999 ◽  
Vol 39 (6) ◽  
pp. 645 ◽  
Author(s):  
W. J. Wales ◽  
P. T. Doyle ◽  
D. W. Dellow

Summary The nylon bag technique was used to estimate the degradability of the dry matter (DM) and crude protein (CP) of herbage samples and supplementary feeds from recent grazing experiments with dairy cows conducted in Victoria. The cumulative disappearance data for CP were used to calculate the effective rumen degradability of CP (ERDP, g/kg DM) for these feeds at rumen outflow rates of 0.02, 0.05 or 0.08/h. Crude protein was more extensively degraded than DM in all herbage samples collected before grazing, or in those samples taken to represent the herbage consumed by grazing cows. The ERDP of spring pastures (excluding the dead material and postgrazed samples) was generally in the range 103–197 g/kg DM, at an outflow rate of 0.02/h and in the range 74–142 g/kg DM, at an outflow rate of 0.08/h. For summer pastures, the ERDP was generally in the range 80–128 g/kg DM, at an outflow rate of 0.02/h and in the range 58–108 g/kg DM, at an outflow rate of 0.08/h. For autumn pastures, ERDP ranged from 108 to 170 g/kg DM, at an outflow rate of 0.02/h and from 84 to 140 g/kg DM, at an outflow rate of 0.08/h. There were only 2 herbage samples collected during the winter, which had ERDP of 237 and 249 g/kg DM, at an outflow rate of 0.02/h and 213 and 222 g/kg DM, at an outflow rate of 0.08/h. Values for ERDP tended to be higher for these winter samples compared with spring samples. In contrast, autumn samples tended to have lower ERDP than spring samples. It is shown that metabolisable protein is unlikely to limit milk production of cows consuming 17 kg herbage DM/cow. day and producing up to 30 L milk/day, when milk production is determined from metabolisable energy supply. The excesses in metabolisable protein ranged from 0.48 to 1.21 kg/cow. day from a subset of spring herbage samples cut to represent that selected by cows and from 0.14 to 0.23 kg/cow. day from summer herbage samples. The degradability characteristics of the herbage samples were similar to some estimates for temperate herbages in the United Kingdom and from limited data from Australia and should form a useful basis on which to assess whether additional metabolisable protein is required as supplements for dairy cows grazing pastures in Victoria. There was a positive (P<0.01) relationship between CP content and ERDP (R2 = 0.93), across herbage samples indicating it could be possible to estimate ERDP for herbage samples that have been assessed for CP content. There was also a significant (P<0.01) positive relationship between the effective degradability of DM of herbage and ERDP (R2 = 0.77). The relationships between in vitro dry matter digestibility or neutral detergent fibre and ERDP were also significant (P<0.01), but accounted for even less of the variability. The ERDP in cereal grain-based supplements ranged from 87 to 111 g/kg DM, at a outflow rate from the rumen of 0.02/h, compared with 70–92 g/kg DM, at an outflow rate of 0.08/h. The compounded supplements containing varying levels of cereal grain, lupins and cottonseed meal, had effective degradabilities of CP that generally declined as the content of lupins decreased and the content of cottonseed meal increased. However, ERDP increased to 152 g/kg DM because of the increasing CP content with increasing cottonseed meal. These results are compared with previously published estimates of effective degradability of CP for supplementary feeds.


1984 ◽  
Vol 64 (2) ◽  
pp. 435-442 ◽  
Author(s):  
P. L. BURGESS ◽  
J. W. G. NICHOLSON

Total mixed rations containing three levels of crude protein (10, 13 and 16%) were fed ad libitum to a total of 48 cows in midlactation in two experiments. Rations were composed of 60% wilted timothy silage and 40% concentrates on a dry matter basis. Supplemental protein was supplied as soybean meal. Daily nitrogen intake per cow averaged 263, 377 and 510 g in exp. 1, and 305, 435 and 509 g in exp. 2 for the three rations, respectively. Cows fed rations containing 10% crude protein consumed less (P < 0.05) total dry matter and lost more (P < 0.01) body weight than cows fed rations with either 13 or 16% crude protein. Milk production was not affected by protein level in exp. 1 but in exp. 2, 10% crude protein decreased (P < 0.01) actual milk and 4% fat corrected milk yields. Milk fat and protein percentages tended to increase with increasing dietary protein level. Rumen acetate tended to decline while propionate increased (P < 0.05) at higher crude protein levels. Rumen ammonia nitrogen and plasma urea nitrogen levels were closely related to nitrogen intake. Key words: Dairy cows, grass silage, protein, solubility


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