Newcastle disease virus antibodies in apparently healthy commercial layers in Abeokuta, Ogun state, Nigeria

2020 ◽  
Vol 45 (3) ◽  
Author(s):  
F. O. Olufemi ◽  
O. Olatunji ◽  
E. O. Omoshaba
Keyword(s):  
Local Government ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Inhibition Assay ◽  
Ogun State ◽  
Poultry Birds ◽  
Significant Difference ◽  
Commercial Poultry ◽  
Apparently Healthy

Several authors have worked on Newcastle Disease (ND) with respect to the incidence, prevalence and epizootiology of the disease and the antibody status in non-vaccinated birds in Nigeria. However, current information on the antibodies of the Newcastle Disease virus in apparently healthy commercial poultry birds with known vaccination records in Abeokuta Metropolis is scanty. This study was conducted, using Heamagglutination Inhibition assay (HI) technique, to detect Newcastle Disease virus (NDV) antibodies in routinely vaccinated commercial poultry birds in the 3 Local Government Areas (LGAs) of Abeokuta that form the Abeokuta metropolis. Haemagglutinating NDV antibodies were detected in the apparently healthy layers thus indicating a widely circulating NDV in areas. A potency test of the vaccines used on the farms was determined using Heamagglutination test and their values ranged between 2 and 2 . The sero-prevalence of NDVantibodies in the 3 LGAs showed there was no significant difference (p >0.05). Of the 120 sera tested, 82 (68.33%) had detectable NDV antibodies but only 81.67% had HI protective titre of > 2 and 18.33% had low seroconversionwith titre 2 or less.

scholarly journals Serological Survey of Newcastle Disease in Free Ranging Local Chickens in the Federal Capital Territory, Abuja, Nigeria

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Samuel Akawu Anzaku ◽  
Jariath Umoh Umoh ◽  
Paul Ayuba Abdu ◽  
Junaidu Kabir ◽  
Akawu Bala
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Serological Survey ◽  
History Of ◽  
Commercial Poultry ◽  
Free Ranging ◽  
Local Chickens ◽  
Apparently Healthy

A serological survey was carried out in four area councils (Abuja Municipal, Kuje, Gwagwalada, and Kwali) of the Federal Capital Territory (Abuja) to determine the prevalence of antibodies to Newcastle disease virus in local chickens using haemagglutination inhibition (HI) tests. In each area council, one hundred sera samples were collected from apparently healthy local chickens with no history of vaccination. Abuja Municipal, Kuje, Gwagwalada, and Kwali area councils had prevalence of 37, 44, 79, and 68%, respectively. The overall prevalence of antibody to Newcastle disease in the four area councils was 57%. This study shows that Newcastle disease virus is circulating in local chickens in the study area, and this may pose a serious threat to the commercial poultry industry within these four area councils of the Federal Capital Territory (Abuja) where this study was carried out.

scholarly journals Molecular epidemiology of Newcastle disease virus isolates from vaccinated commercial poultry farms in non-epidemic areas of Japan

2013 ◽  
Vol 10 (1) ◽  
pp. 330 ◽  
Author(s):  
Dennis Umali ◽  
Hiroshi Ito ◽  
Terumasa Suzuki ◽  
Kazutoshi Shirota ◽  
Hiromitsu Katoh ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Molecular Epidemiology ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Poultry Farms ◽  
Commercial Poultry ◽  
Virus Isolates

scholarly journals Environmental Air Sampling to Detect Exotic Newcastle Disease Virus in Two California Commercial Poultry Flocks

2005 ◽  
Vol 17 (2) ◽  
pp. 198-200 ◽  
Author(s):  
Sharon K. Hietala ◽  
Pamela J. Hullinger ◽  
Beate M. Crossley ◽  
Hailu Kinde ◽  
Alex A. Ardans
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Effective Means ◽  
Air Sampling ◽  
Cost Effective ◽  
Poultry House ◽  
Air Sampler ◽  
Commercial Poultry ◽  
Environmental Air

The 2002–2003 Exotic Newcastle Disease (END) outbreak in Southern California poultry provided an opportunity to evaluate environmental air sampling as an efficient and cost-effective means of sampling flocks for detection of a circulating virus. Exotic Newcastle Disease virus was detected by real-time reverse transcriptase PCR from air samples collected using a wetted-wall cyclone-style air sampler placed within 2 m of birds in 2 commercial flocks suspected of being naturally exposed to END virus during the outbreak. Exotic Newcastle Disease virus was detected after 2 hours of air sampling the poultry-house environments of the 2 naturally infected flocks.

scholarly journals Complete Genome Sequence of a Genotype XVII Newcastle Disease Virus, Isolated from an Apparently Healthy Domestic Duck in Nigeria

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ismaila Shittu ◽  
Poonam Sharma ◽  
Tony M. Joannis ◽  
Jeremy D. Volkening ◽  
Georgina N. Odaibo ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Protein Gene ◽  
Domestic Duck ◽  
Apparently Healthy

The first complete genome sequence of a strain of Newcastle disease virus (NDV) of genotype XVII is described here. A velogenic strain (duck/Nigeria/903/KUDU-113/1992) was isolated from an apparently healthy free-roaming domestic duck sampled in Kuru, Nigeria, in 1992. Phylogenetic analysis of the fusion protein gene and complete genome classified the isolate as a member of NDV class II, genotype XVII.

scholarly journals Preparation and bioactivity of anti-Newcastle disease virus-phosphoprotein cytoplasmic transduction peptide antibody

2020 ◽  
Author(s):  
Benqiang Li ◽  
Man Wang ◽  
Jianguo Zhu
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Protein Function ◽  
Cell Protection ◽  
Test Analysis ◽  
Hiv Tat ◽  
Significant Difference ◽  
Macromolecular Protein ◽  
And Control

Abstract On the basis of cell-penetrating peptide’s character that it can penetrate cytomembrane and transfer macromolecular protein to cytoplasm so to play biological function, we took the experiments. The fuse penetrating peptide our experiment adoptted is HIV-TAT derived fragment-CTP512, with good transmember effect and distinct cytoplasm-position. In this chapter, the research of transmembrane character was processed first. According to the tests on trans- member protein with different concentrations, the best trans-member concentration is 3µM. Afterwards, we found that the location of trans-member antibody is overlapping with phosphoprotein using indirect immunofluorescence test analysis. According to MTT test, there is no significant difference between CTP fusion protein and control on cell proliferation and viability. TCID50 test was used to detect the protective effect of trans-member antibody on cell. Result showed that trans- member antibody has significant cell protection effect compared to the control in the order: ZL.103>ZL.17>Control. Fluorogenic quantitative PCR result showed that trans- member antibody can disturb the duplication and transcription of Newcastle disease virus. This results not only paved a good way to research the transport of disease related protein, but also provide a splendid tool on protein function research.

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